Clinical and Experimental Allergy最新文献

{"title":"Investigation of the Pathomechanism of Chronic Cough Using an In Vitro Approach","authors":"Umesh Singh, Jonathan A. Bernstein","doi":"10.1111/cea.14628","DOIUrl":"10.1111/cea.14628","url":null,"abstract":"<p>Previous ATP inhalation studies and clinical trials have demonstrated an anti-tussive effect of P2X3 antagonists, supporting the mechanism of purinergic P2X3 receptor activation leading to unexplained chronic cough (UCC). However, the role of TRP receptors in UCC is currently unclear. In this study, an interaction between P2X3 receptors on airway bronchial epithelial cells (BECs) and TRPA1 channels on upper airway nerves was explored in vitro as a potential mechanism for UCC (Figure 1A) [<span>1-3</span>]. It was hypothesised that irritants activating TRP channels can result in ATP release from airway nerves resulting in P2X3 receptor activation on adjacent airway epithelial cells [<span>3-6</span>]. To investigate the role of TRPA1 channel activation of P2X3 receptors, an indirect in vitro cell model was developed to demonstrate whether activation of TRPA1 channels expressed on neuronal cells result in ATP release that subsequently activates P2X3 receptors on adjacent BECs.</p><p>Dorsal root ganglion cells (DRGN, ND8/34 cell line, Sigma), known to express TRPA1 were used as a surrogate for airway neuronal cells [<span>7</span>]. Functional assays were performed on DRGNs to quantify TRPA1 activation using the TRPA1 specific agonist (JT010) by observing a change in fluorescence measured by [Ca<sup>2+</sup>]i of 10% or greater from baseline under confocal microscopy. The DRGNs cultured on poly-Lysine coated plates and loaded with FLUO4, were preincubated in the presence or absence of the TRPA1 antagonist HC-030031 (EMD Milipore) and then treated with a TRPA1-specific agonist (JT010 100 nM, Tocris) (<i>n</i> = 3 experiments). Culture media from the DRGNs cell wells were collected after stimulation and assayed for ATP. ATP release in response to JT010-induced TRPA1 activation and its suppression by the TRPA1-antagonist, HC-030031, were quantified from the cell supernatant on a luminometer using the ATP Bioluminescence Assay Kit HS II (Roche). Additional information about study methods are available in the following repository (DOI 10.5281/zenodo.14244289).</p><p>Human bronchoepithelial cells (BEAS2B, ATCC), were used as a surrogate for airway epithelium. The effect of P2X3-receptor antagonist, MAF-454, in preventing activation of P2X3 receptors were determined by treating cultured BEAS2B cells with ATP disodium 4 μM (Sigma) stabilised with KOH in the presence and absence of MAF-454 preincubation. Differential gene expression (DEGs) in these samples versus untreated controls were determined using TaqMan rat inflammation array (Thermo Fisher). Pathway analysis, and upstream regulator analysis of the DEGs in the ATP-treated samples, compared to the MAF-454 preincubated ATP-treated samples were performed using the Ingenuity Pathway Analysis platform (Qiagen). As this was an in vitro study and did not require patient serum or patient data, no IRB was required.</p><p>Descriptive statistics and t-tests were performed to analyse differences in the average","PeriodicalId":10207,"journal":{"name":"Clinical and Experimental Allergy","volume":"55 3","pages":"247-249"},"PeriodicalIF":6.3,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cea.14628","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “T cells and eosinophils in bronchial smooth muscle cell death in asthma”","authors":"","doi":"10.1111/cea.14627","DOIUrl":"10.1111/cea.14627","url":null,"abstract":"<p>\u0000 <span>K. Solarewicz-Madejek</span>, <span>T. M. Basinski</span>, <span>R. Crameri</span>, <span>M. Akdis</span>, <span>A. Akkaya</span>, <span>K. Blaser</span>, <span>K. F. Rabe</span>, <span>C. A. Akdis</span> &amp; <span>M. Jutel</span>, “ <span>T cells and eosinophils in bronchial smooth muscle cell death in asthma</span>”, <i>Clinical &amp; Experimental Allergy</i> <span>39</span>, no. <span>6</span> (<span>2009</span>): <span>845</span>–<span>855</span>, https://doi.org/10.1111/j.1365-2222.2009.03244.x\u0000 </p><p>The authors apologize for all the inconveniences and confirm that all the experimental results and corresponding conclusions remain unaffected.</p><p>The corrected Figure 4 and Figure 5 are shown as follows.</p>","PeriodicalId":10207,"journal":{"name":"Clinical and Experimental Allergy","volume":"55 3","pages":"281-283"},"PeriodicalIF":6.3,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cea.14627","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations Between Self-Reported Dietary Intake and Atopic Dermatitis Risk in Young Adults From Singapore and Malaysia.","authors":"Jun Jie Lim, Kavita Reginald, Yee-How Say, Mei Hui Liu, Fook Tim Chew","doi":"10.1111/cea.14629","DOIUrl":"https://doi.org/10.1111/cea.14629","url":null,"abstract":"<p><p>This cross-sequential study found that frequent intake of high-fat and high-protein foods was associated with higher odds of atopic dermatitis (AD). However, occasional intake across all three macronutrients significantly lowered AD odds, suggesting that moderation-not strict avoidance-may benefit AD management in allergic populations.</p>","PeriodicalId":10207,"journal":{"name":"Clinical and Experimental Allergy","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food Allergy and Mental Health in Children and Adolescents—The Role of Shared Familial Environment","authors":"Hanna Karim,&nbsp;Cecilia Lundholm,&nbsp;Tong Gong,&nbsp;Bronwyn Brew,&nbsp;Michael Silverman,&nbsp;Catarina Almqvist","doi":"10.1111/cea.14619","DOIUrl":"10.1111/cea.14619","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Evidence suggests a link between food allergy and poor mental health, however, this may be explained by shared genetic and environmental factors. We aimed to investigate the association between food allergy of different severity and mental health in children, and the role of familial factors.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This population-based, longitudinal cohort study is based on the Child and Adolescent Twin Study in Sweden with questionnaire data reported by parents and/or children. Food allergy ‘ever’ and doctor's diagnosis were reported at age 9–12 years, and ≥ 1 recent dispensation of adrenaline was used as a marker for current severe food allergy. Outcomes were identified using validated questionnaires for anxiety; Screen for Child Anxiety Related Disorders (SCARED); Strength and Difficulties Questionnaire (SDQ) and depression; Short Mood and Feelings Questionnaire (SMFQ), Center for Epidemiological Studies Depression Scale (CES-D) and Diagnostic and Statistical manual of Mental Disorders (DSM-IV-MDE) and reported at 9–12, 15 and 18 years of age. Multivariate linear and logistic modelling was applied to the whole cohort and a co-twin control approach to remove confounding by familial factors.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In total, 3039 (8.9%) children had a parent-reported food allergy. Among these, 1292 (43.5%) had non-severe food allergy without diagnosis, 1490 (49%) had non-severe food allergy with diagnosis and 257 (8.5%) had severe food allergy. Compared to children with no food allergy, non-severe food allergy with diagnosis by 9–12 years was associated with parent-reported anxiety/depression; SCARED (adjOR 2.10, 95% CI 1.48–2.98), SMFQ (adjOR 1.92, 95% CI 1.19–3.10) at 9–12 years and SDQ (adj<i>β</i> 0.2, 95% CI 0.0–0.4) at 15 years. All other associations were null including for those with severe food allergy. All positive estimates in the full cohort were attenuated using co-twin controls.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Evidence associating paediatric food allergy severity and poor mental health was weak, and positive associations observed were likely due to familial confounding.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10207,"journal":{"name":"Clinical and Experimental Allergy","volume":"55 2","pages":"175-186"},"PeriodicalIF":6.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cea.14619","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advocacy in Action: International Patient Group Improves Hereditary Angioedema Diagnosis and Care Across the Asia-Pacific.","authors":"Jane C Y Wong, Cheryl C W Tsui, Kristie C W Lao, Jovilia Abong, Adli Ali, Dharmagat Bhattarai, Michihiro Hide, Ankur Jindal, Anthony Jordan, Hye-Ryun Kang, Constance H Katelaris, Narissara Suratannon, Sze-Chin Tan, Yong-Hao Lim, Deborah Corcoran, Fiona Wardman, Henrik Balle Boysen, Anthony J Castaldo, Philip H Li","doi":"10.1111/cea.14623","DOIUrl":"https://doi.org/10.1111/cea.14623","url":null,"abstract":"<p><p>This study demonstrates that patient advocacy groups significantly enhance medication availability and improve diagnosis of hereditary angioedema (HAE), particularly in emerging economies within the Asia-Pacific region. This study supports integrating patient advocacy group involvement into management guidelines, emphasising their role in improving access to diagnostics and treatment for HAE.</p>","PeriodicalId":10207,"journal":{"name":"Clinical and Experimental Allergy","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Scoping Review of Cost Questionnaires Aimed at Measuring the Household Financial Burden of Food Allergy.","authors":"Zoe Harbottle, Michael A Golding, Ayel Luis R Batac, Andrew T Fong, Mê-Linh Lê, Elissa M Abrams, Edmond S Chan, Moshe Ben-Shoshan, Peter S Hsu, Jodi A Shroba, Juho E Kivistö, Matthew J Greenhawt, Gregory Mason, Mika J Mäkelä, Antonella Muraro, Staffan Ahlstedt, Jennifer L P Protudjer","doi":"10.1111/cea.14622","DOIUrl":"https://doi.org/10.1111/cea.14622","url":null,"abstract":"","PeriodicalId":10207,"journal":{"name":"Clinical and Experimental Allergy","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142945706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How to Recognise Mastocytosis?","authors":"Clive Grattan,&nbsp;Sigurd Broesby-Olsen,&nbsp;Knut Brockow","doi":"10.1111/cea.14608","DOIUrl":"10.1111/cea.14608","url":null,"abstract":"","PeriodicalId":10207,"journal":{"name":"Clinical and Experimental Allergy","volume":"55 1","pages":"14-18"},"PeriodicalIF":6.3,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fatal Food Anaphylaxis in Children: A Statutory Review in England","authors":"Sylvia Stoianova,&nbsp;Vibha Sharma,&nbsp;Robert J. Boyle","doi":"10.1111/cea.14614","DOIUrl":"10.1111/cea.14614","url":null,"abstract":"&lt;p&gt;Fatal anaphylaxis is a very rare, unpredictable tragedy. For children and young people, most fatal anaphylaxis is caused by food allergy, and thus, carers of children and young people with food allergy may become preoccupied about the possibility of sudden, unexpected fatal anaphylaxis. Other unexpected causes of death such as severe acute asthma are less rare than fatal anaphylaxis, but the rapidity of fatal food anaphylaxis and its propensity to affect adolescents and young adults generate continued concern and societal interest.&lt;/p&gt;&lt;p&gt;The burden of avoiding known food allergens falls largely on people living with allergies and their families. Thus, an exposure leading to a fatal anaphylactic reaction may inspire fear and guilt in families and also in other carers, caterers and health professionals. Most anaphylactic reactions to food self-revert, with or without medical intervention [&lt;span&gt;1&lt;/span&gt;]. In the past 30 years, several society-wide changes have been made to try to prevent fatal food anaphylaxis. In many countries such as the UK, these include widespread provision of adrenaline autoinjectors, new food allergen labelling laws, increased food allergy diagnostics and improved emergency service awareness of anaphylaxis and its management [&lt;span&gt;2, 3&lt;/span&gt;]. Yet, fatal food anaphylaxis remains as common as it was 30 years ago, suggesting we need to go back a step and learn more about the condition [&lt;span&gt;4&lt;/span&gt;]. Fatal food anaphylaxis is difficult to study prospectively, because it is very rare, unpredictable and usually occurs in the community. It is therefore important that we learn as much as we can from each tragic occurrence, and the recent statutory review of childhood fatal anaphylaxis and asthma provides an opportunity to do this (Figure 1, Table 1).&lt;/p&gt;&lt;p&gt;England is one of very few countries with a national, statutory, multi-professional review of all child deaths [&lt;span&gt;5&lt;/span&gt;]. Since 2019, the National Child Mortality Database (NCMD) collates and analyses information about child deaths. For all deaths under age 18, a comprehensive summary of the circumstances of death and background information from professionals is collated. A final record summarises conclusions of a multi-agency panel documenting contributory, modifiable factors and learning. The latest NCMD thematic report analysed child deaths in England because of asthma or anaphylaxis. It identified key findings and made recommendations for policy, practice and research [&lt;span&gt;6&lt;/span&gt;].&lt;/p&gt;&lt;p&gt;The report documents 54 child deaths from asthma and 19 from anaphylaxis in a 4-year period from 1 April 2019 to 31 March 2023. Many (54%) of the children who died from asthma also had a food allergy. However, the cause of death in these cases was thought to be asthma rather than food anaphylaxis. Fatal anaphylaxis was triggered by food allergy (&lt;i&gt;n&lt;/i&gt; = 18) and in one case by anaesthesia, a rare cause of fatal anaphylaxis in childhood [&lt;span&gt;7&lt;/span&gt;]. Just over half of ","PeriodicalId":10207,"journal":{"name":"Clinical and Experimental Allergy","volume":"55 1","pages":"4-7"},"PeriodicalIF":6.3,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cea.14614","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142945710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of MicroRNAs in Allergy and Basophil Activation Test for IgE-Mediated Drug Allergy","authors":"Mohamed H. Shamji,&nbsp;Robert J. Boyle","doi":"10.1111/cea.14616","DOIUrl":"10.1111/cea.14616","url":null,"abstract":"&lt;p&gt;In this month's editorial, the Editors of the journal have highlighted two fascinating studies that are included in this issue. The first article provides evidence that miR-107 is involved in the allergic response to house dust mites (HDM) in children with asthma [&lt;span&gt;1&lt;/span&gt;]. Allergic asthma (AA) is a prevalent phenotype of asthma that presents atopic sensitisations in asthma patients exposed to allergens [&lt;span&gt;2, 3&lt;/span&gt;]. Some, but not all, studies have suggested a possible increase in AA over time [&lt;span&gt;4&lt;/span&gt;]. HDM are a significant trigger for AA in many regions, with HDM sensitisation potentially leading to severe, and in some instances, life-threatening asthma symptoms. In the study by Kim et al. [&lt;span&gt;1&lt;/span&gt;], the relationship between microRNAs (miRNAs) and HDM sensitisation in children with asthma was investigated. The researchers examined serum samples from 1126 children in the Genetics of Asthma in Costa Rica Study (GACRS) and also replicated their findings in the Childhood Asthma Management Program (CAMP). Initially, the study revealed that 17 miRNAs were differentially expressed between HDM-sensitised and non-sensitised children in the GACRS group. Of the 17, miR-642a, let-7c-5p and miR-107 showed the strongest association with HDM sensitisation. Moreover, the CAMP cohort successfully replicated the elevated expression of miR-107 in HDM-sensitised children. Additional mediation analysis also presented significant effects of miR-107 on eosinophil count and total IgE after HDM sensitisation. These findings suggest new insights into the molecular mechanisms underlying HDM sensitisation in paediatric asthma and emphasise the potential of miRNAs, particularly, miR-107, which can be studied further for application as biomarkers or therapeutic targets for AA (Figure 1).&lt;/p&gt;&lt;p&gt;This issue's second editor's choice article reports on the optimisation of the basophil activation assay for identifying IgE-mediated drug allergies [&lt;span&gt;5&lt;/span&gt;]. Diagnosing drug allergies is a complex and nuanced process that presents several challenges. Allergic reactions can manifest with a broad spectrum of clinical symptoms, ranging from mild rashes to life-threatening anaphylaxis, making it difficult to establish consistent diagnostic criteria. Reactions may occur immediately or be delayed, sometimes appearing days after exposure [&lt;span&gt;6&lt;/span&gt;]. This variability complicates the temporal connection between drug intake and allergic symptoms. Additionally, patients may experience allergic reactions to drug molecules with similar structures, further complicating the identification of the exact trigger, especially in cases involving structurally similar drugs. Unlike testing for environmental allergens, there are limited standardised and clinically validated laboratory tests available for many drug allergies. The reliability and availability of tests like the Basophil Activation Test (BAT) can vary for different drugs [&lt;span&gt;7, 8&lt;/span&gt;]. While o","PeriodicalId":10207,"journal":{"name":"Clinical and Experimental Allergy","volume":"55 1","pages":"11-13"},"PeriodicalIF":6.3,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cea.14616","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Featured Cover","authors":"Shican Zhou,&nbsp;Ju Lai,&nbsp;Na Che,&nbsp;Kai Fan,&nbsp;Chuanliang Zhao,&nbsp;Bojin Long,&nbsp;Chunyan Yao,&nbsp;Yu Zeng,&nbsp;Shaoqing Yu","doi":"10.1111/cea.14621","DOIUrl":"https://doi.org/10.1111/cea.14621","url":null,"abstract":"<p>The cover image is based on the article <i>Emerging Role of SAMSN1⁺Mast Cells: Insights From Mendelian Randomisation and Transcriptomic Analyses on Chronic Sinusitis and Obesity</i> by Shaoqing Yu et al.,\u0000https://doi.org/10.1111/cea.14529.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":10207,"journal":{"name":"Clinical and Experimental Allergy","volume":"55 1","pages":"i"},"PeriodicalIF":6.3,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cea.14621","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143112862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}