Clinical and Experimental Dental Research最新文献

{"title":"Interkingdom Endodontic Biofilm Supernatant Induces a Biphasic Inflammatory and Metabolic Transcriptional Response in Dental Pulp Stem Cells In Vitro","authors":"Saeed S. Alqahtani,&nbsp;Sumaya Abusrewil,&nbsp;Othman Baradwan,&nbsp;Om Alkhir Alshanta,&nbsp;Ryan Kean,&nbsp;William McLean,&nbsp;Jason L. Brown,&nbsp;Christopher J. Nile","doi":"10.1002/cre2.70417","DOIUrl":"10.1002/cre2.70417","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To investigate how soluble byproducts derived from a four-species endodontic biofilm model impact the viability, transcriptomic profile, and inflammatory response of human dental pulp stem cells (DPSCs).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A sterile-filtered supernatant was extracted from an established interkingdom endodontic biofilm model comprising <i>Streptococcus gordonii</i>, <i>Fusobacterium nucleatum</i>, <i>Porphyromonas gingivalis,</i> and <i>Candida albicans</i>. DPSCs were exposed to the microbial biofilm supernatant (BSN) for 4 and 24 h. Cellular responses were evaluated via MTT, CCK-8, LDH assays, and Annexin V/PI staining. Transcriptomic sequencing was performed to assess gene expression dynamics, with GO and KEGG pathway enrichment analyses. IL6 and IL8 expression was validated by qPCR and ELISA. Data were analyzed using t-tests/ANOVA and RNA-seq differential expression using DESeq. 2 with FDR adjustment.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>BSN significantly suppressed DPSC metabolic activity without inducing apoptosis or necrosis. RNA-seq revealed 723 significantly differentially expressed genes at 4 h and 1667 at 24 h. Early responses were dominated by upregulation of inflammatory mediators, with enrichment of TNF, NF-κB, and JAK-STAT signaling pathways. At 24 h, the expression profile shifted toward redox regulation and metabolic suppression, including downregulation of glycolytic and purine metabolism pathways. IL6 and IL8 expression was markedly increased at both transcript and protein levels.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Soluble factors produced by a biofilm model representative of deep caries and carious pulp exposures induce a time-dependent transcriptional response in DPSCs. This response is characterized by a biphasic pattern of early immune activation followed by later transcriptional metabolic adaptation. These findings highlight the capacity of soluble biofilm-derived products associated with deep caries to modulate DPSC immune–metabolic signaling. They further emphasize the importance of vital pulp therapy strategies that not only target microorganisms but also account for their secreted byproducts.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13380816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148497203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the Workflows in Dynamic and Robotic Computer-Assisted Implant Surgery","authors":"Adria Jorba-Garcia,&nbsp;Shengchi Fan,&nbsp;Feng Wang,&nbsp;James Chow,&nbsp;Atiphan Pimkhaokham,&nbsp;Nikos Mattheos,&nbsp;Alessandro Pozzi","doi":"10.1002/cre2.70419","DOIUrl":"https://doi.org/10.1002/cre2.70419","url":null,"abstract":"<p>Computer-assisted implant surgery (CAIS) represents a comprehensive digital workflow integrating prosthetically driven planning with various execution modalities. Among these, dynamic (d-CAIS) and robotic (r-CAIS) systems constitute two distinct yet interrelated approaches, sharing core technological principles as well as operative differences. This white paper aims to provide a structured overview of the fundamental concepts and workflows underpinning d- and r-CAIS, focusing on their shared components, procedural steps, and system-specific characteristics. Both approaches rely on accurate digital treatment planning, spatial positioning systems, registration protocols, and real-time feedback mechanisms to guide implant placement. However, while d-CAIS enables operator-controlled freehand execution under real-time navigation, r-CAIS introduces robotic assistance or task autonomy, integrating mechanical actuation into the surgical workflow. Rather than providing exhaustive technical instructions, this work aims to establish a conceptual framework to support clinicians in understanding and evaluating emerging CAIS technologies and their role in contemporary implant dentistry. Furthermore, it discusses current advantages, limitations, and challenges related to clinical implementation, cost, and training requirements.</p>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70419","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148467177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial Effect of Iodine-Based Paste With Silver Nanoparticles Against Enterococcus faecalis: An In Vitro Study","authors":"Carbajal-Bastida Sarasuadi,&nbsp;Robles-Bermeo Norma Leticia,&nbsp;Guadarrama-Reyes Saraí Carmina,&nbsp;Morales-Luckie Raúl Alberto,&nbsp;María Guadalupe González-Pedroza,&nbsp;Bermeo-Escalona Josué Roberto,&nbsp;Carlo Eduardo Medina Solís","doi":"10.1002/cre2.70410","DOIUrl":"https://doi.org/10.1002/cre2.70410","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Pulpectomy is one of the most common treatments for the rehabilitation and conservation of primary dentition. This procedure requires thorough disinfection of the root canals to eliminate the bacterial load prior to obturation. However, the anatomical complexity of primary root canals limits the complete eradication of certain bacteria, such as <i>Enterococcus faecalis</i> (<i>E. faecalis</i>). Although iodine-based pastes, such as Vitapex, are considered the gold standard for primary root canal fillings due to their excellent clinical outcomes, the extent of their antibacterial efficacy remains unclear.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To evaluate the antibacterial efficacy of an iodine-based paste incorporated with silver nanoparticles against <i>E. faecalis</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Design</h3>\u0000 \u0000 <p>This experimental study employed a comparative, cross-sectional, analytical, and prospective design.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Silver nanoparticles (AgNPs) were synthesized and chemically characterized prior to incorporation into an iodoform paste (Vitapex). The antibacterial efficacy was subsequently evaluated across three distinct groups: Group 1 (Vitapex), Group 2 (AgNPs), and Group 3 (Vitapex with AgNPs). Experiments were performed in triplicate using the <i>E. faecalis</i> ATCC 29212 strain to measure the zones of inhibition for each group.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Significant statistical differences were observed (<i>p</i> = 0.003) in the inhibition zones against <i>E. faecalis</i>. The antibacterial activity of the <b>Vitapex+AgNPs</b> combination (SD 13.93 ± 0.83) was significantly higher than that of <b>Vitapex</b> (SD 10.26 ± 0.88) and <b>AgNPs</b> (SD 11.15 ± 2.40) alone.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our results demonstrate that both Vitapex and AgNPs exhibit antibacterial activity against <i>E. faecalis</i>; furthermore, their combined application shows a superior synergistic effect.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70410","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148467281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concordance of a Structured Orthopantomogram Reporting Tool Between Dental Practitioners, and Compared to Routine Reports Within a Regional and Remote Service","authors":"Stevie Dilley,&nbsp;Lynlee Tatnell,&nbsp;Paul Monsour,&nbsp;Jacob O'Gorman,&nbsp;Megan J. Hobbs","doi":"10.1002/cre2.70366","DOIUrl":"https://doi.org/10.1002/cre2.70366","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Variability in interpreting orthopantomograms can have greater impact on regional and remote patients who experience poorer oral health, have reduced service access and incur greater costs, if referred to out-of-community services. Structured orthopantomogram reporting and specialist advice pathways may support more consistent image interpretation and clinical decision-making. This study evaluated the agreement of a structured orthopantomogram reporting tool between dentists and compared the information recorded using the tool with routine clinical reports.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Study Design</h3>\u0000 \u0000 <p>Pilot inter-rater concordance study.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>The inter-rater and inter-method agreement relating to image quality, radiographic features, and the need for specialist review were assessed across 50 orthopantomogra.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The structured tool was completed, on average, in 10.25 min (SD = 8.62), and yielded almost perfect agreement between the two dentists (<i>κ</i>(95%CI) = 0.89 [0.86–0.92]). Agreement across radiographic features ranged from substantial to perfect agreement, with lower agreement observed for whether a specialist referral was needed. Inter-method agreement between the structured tool and routine reports was fair (<i>κ</i>(95%CI) = 0.33 [0.29–0.37]), likely reflecting the greater level of detail prompted by the structured tool rather than differences in the quality of routine reporting practices. Half of the orthopantomograms were reviewed by a specialist, who determined only 6% warranted a referral.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Structured reporting supports more consistent interpretation of orthopantomograms. A formalised specialist advice pathway may reduce unnecessary referrals to out-of-community services, and support dentists' confidence and consistency when completing orthopantomogram reports.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70366","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148467279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Air Polishing and Ultrasonics on Veneer Margins in Vitro: An Optical Coherence Tomography Pilot Study","authors":"Julia Moeller,&nbsp;Tobias Meissner,&nbsp;Florian Fuchs,&nbsp;Andreas Koenig,&nbsp;Sebastian Hahnel,&nbsp;Laura Antonia Mayer,&nbsp;Lena Unterschuetz,&nbsp;Nadia Oberueck,&nbsp;Rainer Haak,&nbsp;Dirk Ziebolz,&nbsp;Ellen Schulz-Kornas","doi":"10.1002/cre2.70407","DOIUrl":"https://doi.org/10.1002/cre2.70407","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>In vitro evaluation of substance loss at the restoration margin of veneers was conducted as a function of prophylaxis procedures, air polishing, or ultrasonic instrumentation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Forty-eight extracted premolars were restored with CAD/CAM materials after veneer preparation (<i>t</i>₀): lithium disilicate, zirconia-reinforced lithium silicate ceramics, and resin-based composite. Five cycles of prophylaxis treatment (air polishing or ultrasonic scaling) and thermocycling (<i>t</i>_E) were conducted. The substance loss of the luting material at the restoration margin was evaluated regarding width, depth, and the veneer inclination angle using optical coherence tomography and contour analysis. Group differences were determined using two-factor analysis of variance, Tukey tests, and linear regression models.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Both prophylaxis procedures lead to substance loss at the restoration margin. The material choice had a significant effect on width and depth at the veneer margin (<i>p</i> &lt; 0.002), this was not observed for the prophylaxis procedures (<i>p</i> ≥ 0.445). Air polishing resulted in the largest reduction in width and depth in zirconia-reinforced lithium silicate. A cross-group influence of inclination angle on substance loss was not detected.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Both prophylactic procedures cause substance loss at the margins of the investigated CAD/CAM ceramic as well as composite veneers in the area of adhesive bonding.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Clinical Significance</h3>\u0000 \u0000 <p>The choice of luting and veneer material seems to have a more significant influence on the extent of substance loss than the type of instrumentation of the prophylaxis procedure.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70407","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148467280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding Oral Hygiene and Periodontal Health in Space Using a Bedrest Analog Model for Weightlessness (NASA Sensorimotor Countermeasures Study)","authors":"Sonja Henny Maria Derman,&nbsp;Laura de Boni,&nbsp;Edwin Mulder,&nbsp;Stefan Möstl,&nbsp;Julia Jagel,&nbsp;Teresa Kruse,&nbsp;Anna Greta Barbe","doi":"10.1002/cre2.70412","DOIUrl":"https://doi.org/10.1002/cre2.70412","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>To investigate longitudinal alterations in oral hygiene, gingival inflammation, and salivary secretion during a 60-day head-down tilt bed rest (HDTBR) protocol as a validated ground-based analog of microgravity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Twenty-four healthy volunteers participated in a 60-day, 6° HDTBR study conducted under strictly controlled conditions. Oral health assessment safety measures were performed at baseline, mid-head-down tilt (HDT), end-HDT, and post-recovery.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Plaque accumulation and gingival inflammation increased, with increases from baseline to end-HDT in the Quigley-Hein Plaque Index (1.8 ± 0.4 to 2.3 ± 0.4; <i>p</i> &lt; 0.01), Gingival Index (0.8 ± 0.4 to 1.2 ± 0.2; <i>p</i> &lt; 0.001), and bleeding on probing (BoP) (18.4 ± 11.6% to 35.1 ± 16.6%; <i>p</i> &lt; 0.001). BoP partially recovered post-HDT, while QHI and GI remained elevated compared with baseline. Periodontal Screening Index scores shifted toward higher grades during HDT without evidence of destructive periodontal breakdown. The unstimulated salivary flow rate remained unchanged throughout.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Prolonged gravitational unloading induces a plaque-driven deterioration of gingival health in systemically healthy individuals, likely related to impaired mechanical plaque control and microcirculatory changes rather than salivary dysfunction. Gingival tissues appear to be sensitive biomarkers of adaptation to unloading conditions, supporting the need for optimized oral hygiene and adjunctive preventive strategies during future long-duration space missions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Trial Registration</h3>\u0000 \u0000 <p>German Clinical Trial Registry (DRKS00033882, registration date 03-15-2024, https://www.drks.de/search/de/trial/DRKS00033882/details).</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70412","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148467284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Initial Cytotoxicity of Additively and Subtractively Manufactured Resin-Based Materials for Interim Fixed Dental Prostheses: An In Vitro Study","authors":"José M. Alegre,&nbsp;Jesús Peláez,&nbsp;Aránzazu Sánchez,&nbsp;Blanca Herrera,&nbsp;Seyed Ali Mosaddad,&nbsp;Pedro Diaz,&nbsp;María J. Suárez","doi":"10.1002/cre2.70413","DOIUrl":"https://doi.org/10.1002/cre2.70413","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To compare the initial cytotoxic response of additively manufactured (AM) and subtractively manufactured (SM) resin-based materials for interim fixed dental prostheses (FDPs) under indirect and direct in vitro exposure conditions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>A total of 216 standardized resin discs were fabricated from AM resins (Group P, <i>n</i> = 108) and SM polymethyl methacrylate (PMMA)-based resins (Group M, <i>n</i> = 108). L929 mouse fibroblasts were used as the biological model, with a negative control group (Group C, <i>n</i> = 36). After 24 h of cell attachment, cell viability was assessed after 72 h under two conditions: indirect exposure to resin eluates and direct contact with resin discs. Viability was quantified by crystal violet staining. Data were analyzed using Kruskal–Wallis tests followed by Dunn's pairwise post hoc comparisons (<i>α</i> = 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In the indirect assay, Group M showed 87% cell survival relative to the control, whereas Group P showed 65%; in the direct assay, values were 89% and 48%, respectively. Significant differences were observed among pooled groups in both assays (<i>p</i> &lt; 0.001). Based on ISO 10993-5 thresholds, Group P was classified as slightly cytotoxic in the indirect assay and moderately cytotoxic in the direct assay, whereas Group M remained within the non-cytotoxic to slightly cytotoxic range. Group P showed significantly lower cell viability than Group M in both assays (adjusted <i>p</i> &lt; 0.001), while no statistically significant differences were detected within each manufacturing category (adjusted <i>p</i> &gt; 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>At 72 h, the tested AM resins showed significantly lower cell viability than the tested SM PMMA-based resins under the present in vitro conditions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Clinical Significance</h3>\u0000 \u0000 <p>The lower cell viability observed for the tested 3D-printed interim materials under short-term in vitro conditions highlights the need for caution when extrapolating 72-h cell-viability findings to clinical performance and supports further standardized long-term in vitro and in vivo investigations.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70413","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148467282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Implant Type on Clinical and Radiologic Outcomes: A Cross-Sectional Private Practice-Based Analysis","authors":"Cristian Scognamiglio,&nbsp;Gülce Çakmak,&nbsp;Martin Schimmel,&nbsp;Alessandro Perucchi,&nbsp;Samir Abou-Ayash","doi":"10.1002/cre2.70411","DOIUrl":"https://doi.org/10.1002/cre2.70411","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To compare tissue-level (TL) and bone-level X (BLX) implants regarding peri-implant bone level alterations, survival, and complication rates over a 3-years.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>In this retrospective, non-interventional clinical study, patients that had received either TL or BLX implants (both: Straumann AG), with at least one clinical follow-up (minimum 6 months), were included. Bone-level alterations and bone peaks were evaluated at baseline (implant placement) and at follow-ups from dental radiographs. In addition, clinical follow-ups included the recording of the presence of keratinized mucosa and the registration of technical complications (e.g., chipping fractures). For statistics, descriptive analyses, <i>t</i>-tests, and a random-effects linear regression analysis were applied.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 146 implants (73 TL and 73 BLX) were included. TL implants showed a survival rate of 100%, whereas BLX implants showed a survival rate of 98% after 3 years. TL implants showed significant mesial bone gain (0.08 mm, <i>p</i> = 0.018) between 2- and 3-year follow-ups, with a notable difference (0.21 mm, <i>p</i> = 0.041) compared to BLX implants. At distal bone levels, TL implants showed initial bone loss (0.12 mm, <i>p</i> = 0.020) at 6 months but gained bone (0.07 mm, <i>p</i> &lt; 0.001) between 2 and 3 years, whereas BLX implants showed significant distal bone loss (0.15 mm, <i>p</i> = 0.038) over 3 years. The number of complications was higher in BLX implants. BLX implants with a band of keratinized mucosa &gt; 2 mm showed smaller bone level alterations.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Both TL and BLX implants performed similarly in terms of clinical and radiological outcomes. However, at the mesial aspect, bone level alterations in TL implants were smaller than in BLX implants, and complications were less frequent. Keratinized mucosa seems especially beneficial in BLX implants. As implant selection was based on clinical indication, the two groups differed in baseline characteristics, and this should be considered when interpreting the between-group comparisons.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70411","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148467283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Porphyromonas gingivalis Infection on PGC-1α in Skeletal Muscle After Endurance Training In Vivo","authors":"Kairi Hayashi,&nbsp;Yasuo Takeuchi,&nbsp;Hiroaki Kobayashi,&nbsp;Gen Tanabe,&nbsp;Hiroshi Churei,&nbsp;Toshiaki Ueno,&nbsp;Kenji Fueki","doi":"10.1002/cre2.70415","DOIUrl":"https://doi.org/10.1002/cre2.70415","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>It is well known that <i>Porphyromonas gingivalis</i>, a major pathogen in periodontitis, causes systemic diseases. This study investigated the expression of PGC-1α (the most important factor in muscle mitochondria synthesis) following endurance training to clarify the relationship between <i>P. gingivalis</i> infection and muscle endurance capacity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Twenty 8-week-old Wistar rats were randomly divided into two groups. One group was intraperitoneally administered sonicated <i>P. gingivalis</i> (<i>P. g</i>-group), and the other group was administered saline (Control-group). Three weeks after administration, gastrocnemius muscles and blood from both groups were collected 24 h after treadmill exercise. The expressions of PGC-1α and TNF-α in gastrocnemius muscle were measured, along with serum antibody titers for IgG, in response to <i>P. gingivalis</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Serum IgG antibody titers and TNF-α expression in skeletal muscle were significantly higher in the <i>P. g</i>-group than in the Control-group. PGC-1α expression was significantly lower in the <i>P. g</i>-group than in the Control-group. A correlation was observed between PGC-1α and TNF-α expression.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our result suggests that the increase in endurance following training may be suppressed by <i>P. gingivalis</i> infection.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70415","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148467285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness in Remineralizing White Spot Lesions in Primary Teeth With Varnishes: A Systematic Review","authors":"Carolina Caleza-Jiménez,&nbsp;María Biedma-Perea,&nbsp;David Ribas-Pérez,&nbsp;Maria José Barra-Soto,&nbsp;Marcela Arenas-González","doi":"10.1002/cre2.70403","DOIUrl":"10.1002/cre2.70403","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This systematic review aims to assess the effectiveness of varnishes for the remineralization of white spot lesions in primary teeth.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A database search was conducted in MEDLINE and the Cochrane Library to screen for articles relevant to the topic of the review. Risks of bias in randomized studies were assessed using the ROB2 tool and GRADE evidence levels.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Seven studies met all inclusion criteria and were included. Differences in varnish composition and characteristics can affect the anticaries properties. Bioactive additives had a statistically significant reduction in ICDAS scores.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Intensive application of remineralizing varnishes demonstrated a significant effect on the remineralization and control of carious lesion activity. New-generation fluoride appear to be more effective than conventional sodium fluoride varnishes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70403","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148410862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}