Eurasian Journal of Applied Biotechnology最新文献_第7页

Distribution and genetic features of the causative agent of Alaria alata in wild carnivores of the steppe and forest-steppe zones of Kazakhstan 哈萨克斯坦草原和森林草原带野生食肉动物阿拉木耳致病菌的分布和遗传特征

Eurasian Journal of Applied Biotechnology Pub Date : 2022-07-09 DOI: 10.11134/btp.2.2022.6

A. Smagulova, R. Uakhit, L. Lider, А.S. Lyalchenko, V. Kiyan

{"title":"Distribution and genetic features of the causative agent of Alaria alata in wild carnivores of the steppe and forest-steppe zones of Kazakhstan","authors":"A. Smagulova, R. Uakhit, L. Lider, А.S. Lyalchenko, V. Kiyan","doi":"10.11134/btp.2.2022.6","DOIUrl":"https://doi.org/10.11134/btp.2.2022.6","url":null,"abstract":"This article presents data on the study of the Alaria spp. pathogen isolated from wild carnivores (corsacs, foxes, wolves) caught in the territory of Karaganda, Kostanay, Akmola, and East Kazakhstan regions in the period from 2019 to 2022. As a result of the autopsy of wild animals, a collection of Alaria spp. was collected, the intensity of invasion varied from 1 to 1489 samples/animal, and the prevalence of invasion ranged from 10 to 36.11%. The primary taxonomic affiliation of the pathogen was carried out using determinants. Genetic identification was carried out by amplification of a section of the internal transcribed spacer 2 (ITS2) marker gene. The resulting 557 bp amplicons. sequenced using the Sanger method. The nucleotide sequences were deposited in the international GenBank database under accession numbers: from the fox - OM630451, ON248128, ON248130, from the corsac - OM630460, ON248043, ON248044, ON248045, from the wolf - ON358106. Bioinformatics analysis to build a phylogeny showed that the samples of Alaria alata studied by us were combined into a separate cluster by identity, which ranged from 90-98%.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132069355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High affinity of recombinant DJ-1 (PARK7) protein to Ni-NTA 重组DJ-1 (PARK7)蛋白对Ni-NTA的高亲和力

Eurasian Journal of Applied Biotechnology Pub Date : 2022-07-09 DOI: 10.11134/btp.2.2022.5

A. Mussakhmetov, D. Utepbergenov, B. Khassenov

引用次数: 0

ISOLATION AND CHARACTERISTICS OF BACTERIOPHAGES SANITARY-INDICATIVE MICROORGANISMS 卫生指示微生物噬菌体的分离与特性研究

Eurasian Journal of Applied Biotechnology Pub Date : 2022-07-09 DOI: 10.11134/btp.2.2022.4

M.A. Monarchovich, O.A. Galaburda, A. Arashkova, N. Sverchkova, E. Kolomiets

引用次数: 0

Genetic variation of soybean collection based on microsatellite DNA markers related to plant height 基于株高相关微卫星DNA标记的大豆种质资源遗传变异研究

Eurasian Journal of Applied Biotechnology Pub Date : 2022-07-09 DOI: 10.11134/btp.2.2022.1

T. Podzorova, A. Zatybekov, S. Didorenko, S. Abugalieva

{"title":"Genetic variation of soybean collection based on microsatellite DNA markers related to plant height","authors":"T. Podzorova, A. Zatybekov, S. Didorenko, S. Abugalieva","doi":"10.11134/btp.2.2022.1","DOIUrl":"https://doi.org/10.11134/btp.2.2022.1","url":null,"abstract":"Soybean is a relatively new crop in Kazakhstan, and the development of new competitive cultivars with high productivity and quality is an important direction in the local breeding program. Previously, one of the major approaches for the development of new cultivars was breeding projects based on the evaluation of key morphological traits, including flowering time and plant height (PH). However, with the development of soybean genetics new molecular tools have emerged, including the development of DNA microsatellites, or simple sequence repeats (SSRs). In this study, a collection of hundred accessions grown in the southeast of Kazakhstan in 2020-2021 was assessed using eight agronomic traits, including plant height (PH), the height of lowest pod, number of lateral branches, number of fertile nodes, number of seeds per plant, thousand seeds weight, yield per square meter (YP2), and maturity group. The assessment of field trials showed a wide range of variability in agronomic traits and suggested that PH is significantly correlated with all studied traits, including YP2. The collection was genotyped using eight SSRs associated with PH. The results suggested that three SSRs (Satt244, Satt288, and Satt371) were the most polymorphic, and five out of eight SSRs were associated with six important agronomic traits, including flowering time, PH, and yield per square meters. Obtained results can be efficiently used in soybean breeding based on a marker-assisted selection approach.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129410834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA barcoding of rare and endangered plant species of the Republic of Kazakhstan 哈萨克斯坦共和国稀有和濒危植物物种的DNA条形码

Eurasian Journal of Applied Biotechnology Pub Date : 2022-07-09 DOI: 10.11134/btp.2.2022.3

A. Sarsen, M. Saginova, S. Aktayeva, Z. Akishev, A. Kiribayeva, S. Manabayeva, A. Mussakhmetov, A. Kakimzhanova, O. Khapilina, I. Akhmetollayev, B. Khassenov

引用次数: 0

INFLUENCE OF CONSUMPTION OF PROBIOTIC DAIRY YOGURT ON GUT MICROBIOME STRUCTURE 食用益生菌乳酸奶对肠道菌群结构的影响

Eurasian Journal of Applied Biotechnology Pub Date : 2022-07-09 DOI: 10.11134/btp.2.2022.2

Zh. Pernebek, D. Babenko, D. Tarzhanova, I. Uyzbaeva, Zh. Khassenbekova, S. Kozhakhmetova, A. Kushugulova, S. Kozhakhmetov

引用次数: 0

DISTRIBUTION OF ERWINIA AMYLOVORA ON WILD AND CULTURALLY GROWN APPLE TREES MALUS SPP. FOR THE PERIOD OF 2021 2021年野生和栽培苹果树上淀粉状欧文菌的分布

Eurasian Journal of Applied Biotechnology Pub Date : 2022-07-09 DOI: 10.11134/btp.2.2022.9

N. Malakhova, Y. Skiba, B. Tezekbayeva, A. Khasseyn, K. Dmitriyeva, V.N. Romashkin, G. Zharmukhamedova, Zh. K. Jumanova, E. Maltseva

{"title":"DISTRIBUTION OF ERWINIA AMYLOVORA ON WILD AND CULTURALLY GROWN APPLE TREES MALUS SPP. FOR THE PERIOD OF 2021","authors":"N. Malakhova, Y. Skiba, B. Tezekbayeva, A. Khasseyn, K. Dmitriyeva, V.N. Romashkin, G. Zharmukhamedova, Zh. K. Jumanova, E. Maltseva","doi":"10.11134/btp.2.2022.9","DOIUrl":"https://doi.org/10.11134/btp.2.2022.9","url":null,"abstract":"Fire blight of pome fruit cultures is a dangerous quarantine disease of pome and ornamental plants of Rosaceae, caused by a Gram-negative bacterium Erwinia amylovora. For the study of the fire blight distribution and timely establishment of the threat of endemic apple species Malus sieversii infection a phytopathological study of wild fruit forests and cultural plantations of apple (Malus spp.) was carried out in Almaty region. 263 samples were collected from wild plants without visible symptoms and cultivated plants with symptoms of a fire blight - brown and necrotic young shoots that have a characteristic \"shepherd's crook\" shape, blackened leaves and ovaries remaining on the affected shoots, and samples from trees with manifestations of the disease had previously been noted were collected as well. Screening of all received samples was carried out based on PCR analysis and revealed 23 positive results.\u0000Phytopathological observations and the results of PCR analysis allowed identification of the infection’s source and distribution, as well as assessment of the current state of the studied areas of wild fruit forests and adjacent cultural apple plantations in Almaty region for the presence of fire blight’s infection agent.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122056207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COMPOSING BACTERIAL CONSORTIUM AS A BASIS OF PROBIOTIC FEED ADDITIVE FOR CARP FISH SPECIES AND EVALUATION OF ITS PROPHYLACTING 组成菌群作为鲤鱼益生菌饲料添加剂的基础及其预防作用评价

Eurasian Journal of Applied Biotechnology Pub Date : 2022-06-22 DOI: 10.11134/btp.1.2022.7

K. Kantor, I. Proskurnina, A. Arashkova, E. Kolomiets, Z. Koshak, N. Gadlevskaya, E. Rybkina

引用次数: 0

Production of polyclonal antibodies against the DJ-1 protein associated with Parkinson's disease pd -1相关蛋白多克隆抗体的制备

Eurasian Journal of Applied Biotechnology Pub Date : 2022-06-03 DOI: 10.11134/btp.1.2022.5

A. Mussakhmetov, K. Baltin, B. Khassenov

{"title":"Production of polyclonal antibodies against the DJ-1 protein associated with Parkinson's disease","authors":"A. Mussakhmetov, K. Baltin, B. Khassenov","doi":"10.11134/btp.1.2022.5","DOIUrl":"https://doi.org/10.11134/btp.1.2022.5","url":null,"abstract":"Parkinson's disease is a slowly progressive chronic neurodegenerative disease with a global prevalence estimated at 0.3% and rising sharply with age to over 3% in people over 80 years of age. Estimates of the incidence of Parkinson's disease worldwide range from 5 to 35 new cases per 100,000 people per year. The disorder is defined by the presence of classic motor signs, including the characteristic presence of bradykinesia in all patients, tremor in most, and rigidity. One of the proteins associated with Parkinson's disease is DJ-1, also known as Parkinson disease protein 7 (PARK7). Studies have shown that DJ-1 is an antioxidant protein that plays a key role in counteracting oxidative stress in dopamine neurons. In the framework of the studies carried out in Escherichia coli BL21(DE3)-RIPL strain, the recombinant rDJ-1 protein was obtained in an amount of 3.5 mg. By immunizing a rabbit, polyclonal anti-rDJ-1 antibodies were isolated, which, in enzyme immunoassay and Western blotting, showed high sensitivity to the DJ-1 protein at a dilution of up to 1: 512,000. The resulting polyclonal antibodies showed the required informativeness when studying the expression of DJ-1 in the cell lines HEK293 (wild type) and HEK293park- (with knocked-out park gene). The resulting antibodies can be used in studies to assess the expression level of DJ-1 in immunological tests to detect and quantify the target protein: flow cytometry, ELISA, Western blotting, immunofluorescence, immunohistochemistry, immunoelectrophoresis.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"460 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132361232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

SPECIES COMPOSITION OF WOLF (CANIS LUPUS) HELMINTH FAUNA IN KAZAKHSTAN 哈萨克斯坦狼(canis lupus)蠕虫动物群的种类组成

Eurasian Journal of Applied Biotechnology Pub Date : 2022-06-03 DOI: 10.11134/btp.1.2022.6

R. Uakhit, A. Smagulova, L. Lider, S. Leont'ev, A. Berber, V. Kiyan

{"title":"SPECIES COMPOSITION OF WOLF (CANIS LUPUS) HELMINTH FAUNA IN KAZAKHSTAN","authors":"R. Uakhit, A. Smagulova, L. Lider, S. Leont'ev, A. Berber, V. Kiyan","doi":"10.11134/btp.1.2022.6","DOIUrl":"https://doi.org/10.11134/btp.1.2022.6","url":null,"abstract":"The article presents data on the study of the species composition of wolf helminth fauna in the period from 2019 to 2021. A total of 39 wolves (Canis lupus) were examined, and their parasite fauna was assessed. The study identified the following types of helminths Echinococcus granulosus, Taenia krabbei, Dypilidium spp., Mesocestoides spp., Toxascaris leonina, Trichinella nativa, Dirofilaria repens. The extensiveness of the invasion wolves by helminths was relatively high in the western part region (96.5%), and towards the north-central part region, this indicator significantly decreased (65.2%). The average number of helminths was high, with some infected animals carrying several types of parasites. Thus, the intensity of invasion by helminths in wolves was 7.6 specimens per infected individual host. The research aimed to study the endoparasitic fauna of wild wolves using complex classical parasitological and molecular genetic methods. Samples of helminths were differentiated by amplification and sequencing using the marker gene cytochrome oxidase COX1 (GenBank accession number: MT877205, MZ505444, MZ669895, MZ724175). There is a need to assess the distribution of helminths in intermediate and final hosts populations and provide appropriate health education to avoid maintaining parasite life cycles and infestations, which will become a public health problem in the future.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115114206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0