Eurasian Journal of Applied Biotechnology最新文献_第10页

Evaluation Of The Mutagenic Potential Of The Polyphenol Extract From Blueberries In The Ames Test 蓝莓多酚提取物诱变潜力的Ames试验评价

Eurasian Journal of Applied Biotechnology Pub Date : 2021-03-26 DOI: 10.11134/btp.1.2021.3

Z. Shulgau, S. Sergazy, A. M. Zhulikeeva, A. Y. Dautov, A. Gulyayev, L. Kovalenko, L. Belotserkovtseva, N. S. Kavushevskaya, A. V. Logutenko

引用次数: 0

Obtaining Multiprotein Antigens Of Brucella And Study Of Their Immunoreactivity 布鲁氏菌多蛋白抗原的制备及其免疫反应性研究

Eurasian Journal of Applied Biotechnology Pub Date : 2021-03-26 DOI: 10.11134/btp.1.2021.6

B. K. Ingirbai, A. Syzdykova, A. Kurmasheva, A. Bulashev

{"title":"Obtaining Multiprotein Antigens Of Brucella And Study Of Their Immunoreactivity","authors":"B. K. Ingirbai, A. Syzdykova, A. Kurmasheva, A. Bulashev","doi":"10.11134/btp.1.2021.6","DOIUrl":"https://doi.org/10.11134/btp.1.2021.6","url":null,"abstract":"Rose-Bengal test, complement fixation test and the agglutination test are mainly used for the diagnosis of animal brucellosis. These tests are characterized by low sensitivity and specificity, which is one of the main reasons for the low effectiveness of measures aimed at eradicating brucellosis. The use of modern highly sensitive serological tests requires the availability of antigens specific to Brucella spp. The aim of the study was to obtain multiproteins of the pathogen by recombinant DNA technology and to study their antigenic properties. In the course of the study, three types of multiproteins were obtained, constructed from diagnostically important peptides that form B.abortus and B.melitensis outer membrane proteins. All target products were synthesized by the producer strain in a form that is authentic to natural proteins, and showed immunogenicity in mouse model. Antibodies produced against the multiproteins were specific for the single proteins of the pathogen's cell wall. The data obtained indicate the need to continue studies to determine the possibility of using multiproteins as an antigen in an enzyme-linked immunosorbent assay to detect anti-Brucella specific antibodies.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130081728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Modification Of Taq DNA Polymerase with Improved Elongation Ability 提高Taq DNA聚合酶延伸能力的修饰

Eurasian Journal of Applied Biotechnology Pub Date : 2021-03-26 DOI: 10.11134/btp.1.2021.4

A. Sarsen, Z. Akishev, M. Saginova, B. Sultankulov, B. Khassenov

{"title":"Modification Of Taq DNA Polymerase with Improved Elongation Ability","authors":"A. Sarsen, Z. Akishev, M. Saginova, B. Sultankulov, B. Khassenov","doi":"10.11134/btp.1.2021.4","DOIUrl":"https://doi.org/10.11134/btp.1.2021.4","url":null,"abstract":"Thermostable polymerases play a significant role in molecular biology and diagnostic practice. The most famous and demanded is Polymerase I from the thermophilic bacterium Thermus aquaticus (Taq-pol). This polymerase at one time made a kind of revolution in the polymerase chain reaction. In this work, we attempted to modify this polymerase by attaching an additional Sso7d protein from Sulfolobus solfataricus to Taq-pol, which provides additional binding to the double-stranded DNA of the template. Sso7d-Taq fusion gene was expressed in BL21(DE3) cells. Optimal conditions were selected for maximum production of modified Sso7d-Taq polymerase. The optimal conditions for the intracellular accumulation of Sso7d-Taq polymerase: activation of the T7 promoter when the optical density of the culture reaches OD600 = 0.8-1.0 by adding IPTG at a concentration of 0.2 mM, followed by incubation of the culture at 37°C for 20-24 hours. Recombinant Sso7d-Taq polymerase has been purified and tested by PCR for thermal stability and elongation time. It was found that the Sso7d-Taq enzyme withstands 5 hour incubation at 95°C and 75 minute incubation at 98°C. Comparative analysis with unmodified Taq DNA polymerase showed that the Sso7d-Taq enzyme reduces the elongation rate by several times - up to 15-13 seconds per 1 kbp. The results obtained indicate the prospects of using Sso7d-Taq DNA polymerase in scientific research and diagnostic practice.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130615047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molybdoenzyme Participation In Plant Biochemical Processes 钼酸酶参与植物生化过程

Eurasian Journal of Applied Biotechnology Pub Date : 2021-03-26 DOI: 10.11134/btp.1.2021.2

D. Tokasheva, Zhadyrassyn Nurbekova, A. Akbassova, R. Omarov

{"title":"Molybdoenzyme Participation In Plant Biochemical Processes","authors":"D. Tokasheva, Zhadyrassyn Nurbekova, A. Akbassova, R. Omarov","doi":"10.11134/btp.1.2021.2","DOIUrl":"https://doi.org/10.11134/btp.1.2021.2","url":null,"abstract":"Molybdenum is a key microelement in plant vital functioning. The microelement can be absorbed by the plants only in the form of molybdate-anion. The Molybdenum deficiency affects negatively to the most important agricultural growing. As molybdenum takes part in such vital mechanisms as nitrogen and sulfur metabolism, plant hormone biosynthesis, and purine banding catabolism. Molybdenum is included in enzyme content which is called molybdoenzyms. Having bonded with molybdopterin it creates molybdenum co-factor (Moco) and gets oxidation-reduction properties. Moco is included in active site of molybdoenzymes. They take part in sulfur and nitrogen metabolism, and detrimental compound detoxication. Molybdenum deficiency is characterized by the slow plant growth, low amount of chlorophyll ascorbic acid capacity.It was noticed that plants suffering from the molybdenum deficiency can be saved, sodium molibdate can be used, it can be put directly in the soil or plant leaves can sprayed with the solution. There are five plant molibdoenzymes which are currently known: sulfite oxidase (SO), xanthine dehydrogenase (XD), nitrate reductase (NR), aldehyde oxidase (AO) and mitochondrial amidoxim-regenerative component. Nitrate reductase catalyzes the first stage of nitrate assimilation, eucariotic organisms contain three isoforms of the molybdoezimes: A NADH, A NAD(P)H и NADPH. Xanthine dehydrogenase regulates purine metabolism. XD increases plant antioxidant ability and slows down leaves aging. Molybdoenzymess are involved in the process of the stress adaptation, defining of the mechanisms and their reaction to environmental stress conditions is important for plant stress resistance.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"136 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131421760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Study Of Genetic Diversity And Resistance Of Fruit Crops To Main Pathogens Using DNA Markers 利用DNA标记研究水果作物对主要病原菌的遗传多样性和抗性

Eurasian Journal of Applied Biotechnology Pub Date : 2021-03-26 DOI: 10.11134/btp.1.2021.1

Устойчивости Плодовых, Культур К Основным, Патогенам При Помощи, Днк Маркеров

引用次数: 0

The Effect of a Biopreparation on Legume (Pisum Sativum) 一种生物修复剂对豆科植物的影响

Eurasian Journal of Applied Biotechnology Pub Date : 2020-06-01 DOI: 10.11134/BTP.2.2020.12

V. Martynenko, A. Rysbek, A. Kurmanbayev, Zh. A. Baigonusova

引用次数: 0

Isolation and Identification of New Strains of Lactic Bacteria from The Microbiom of Young Carp Fish 幼鱼微生物群中乳酸菌新菌株的分离与鉴定

Eurasian Journal of Applied Biotechnology Pub Date : 2020-06-01 DOI: 10.11134/BTP.2.2020.7

Z. Tekebayeva, G. Bissenova, A. Dossova, M. Urazova, А.В. Shevtsov, Aslan Temirkhanov, А.K. Rakisheva, A. Abzhalelov, Z. Sarmurzina

引用次数: 0

Microbiological Production of Polyhydroxbutyrates From Renewable Sources 微生物利用可再生资源生产聚羟基丁酸酯

Eurasian Journal of Applied Biotechnology Pub Date : 2020-06-01 DOI: 10.11134/BTP.2.2020.3

A. Rysbek, A. Kurmanbayev

{"title":"Microbiological Production of Polyhydroxbutyrates From Renewable Sources","authors":"A. Rysbek, A. Kurmanbayev","doi":"10.11134/BTP.2.2020.3","DOIUrl":"https://doi.org/10.11134/BTP.2.2020.3","url":null,"abstract":"Plastics play an important role in our daily lives and are used for various purposes. The industry of environmentally friendly products is actively developing in our time, including bioplastics, and much attention of scientists is attracted by biodegradable polymers such as polyhydroxyalacanoates or its subspecies polyhydroxybutyrates, which are synthesized by various microorganisms as a reserve substance, and are also the most acceptable replacement for conventional synthetic plastics. However, the cost of large-scale production of such a biodegradable polymer is not competitive with its wide distribution. Studies of the microbial production of polyhydroxybutyrates should be aimed at identifying cost-effective substrates, as well as determining the appropriate strain of the body for production. These biopolymers have a number of specific properties, such as biodegradability and compatibility with living body tissue, which opens up great opportunities for their use in practice. The final product of polyhydroxybutyrates biodegradation in the environment is water and carbon dioxide, and in a living organism 3-butobutyric acid. The main focus of this review was the production of bioplastics from various economical substrates using various types of bacteria.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125061115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Molybdenum on The Activity of Molybdoenzymes 钼对钼酶活性的影响

Eurasian Journal of Applied Biotechnology Pub Date : 2020-06-01 DOI: 10.11134/BTP.2.2020.2

N. Iksat, S. Zhangazin, A. Madirov, R. Omarov

{"title":"Effect of Molybdenum on The Activity of Molybdoenzymes","authors":"N. Iksat, S. Zhangazin, A. Madirov, R. Omarov","doi":"10.11134/BTP.2.2020.2","DOIUrl":"https://doi.org/10.11134/BTP.2.2020.2","url":null,"abstract":"The soil is a reservoir of various contaminants with heavy metals and has a strong cation exchange property. Among these heavy metals, molybdenum is an essential element that is required in small quantities for optimal plant growth and development. This useful heavy metal performs several biochemical and physiological tasks in plants and is also considered as an important component of various cellular enzymes and is actively involved in redox reactions. Mononuclear molybdenum-containing enzymes, as a rule, have a certain conserved metal center, coordinated by one or two pyranopterins. The pyranopterin fragment plays a key role in the properties of the metal site in the group of mononuclear enzymes of molybdenum with various functions: coordination; stabilization and modulation of the redox transitions of the center, acting as a redox buffer; and for redox regulation/compliance in a variety of catalytic reactions. The coordination sphere of the metal is equipped with oxygen and/or sulfur, selenium atoms in various forms. Tungsten is an antagonist of molybdenum and inhibits molybdoenzymes. In the current review we elaborately reviewed various studies regarding heavy metals - molybdenum and tungsten, their uptake mechanism, essential transporters, and also discuss about the destructive properties of heavy metals in response to their concentration.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133358932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In Vitro and In Vivo Effects of Radio- Frequency Electromagnetic Fields 射频电磁场的体内外效应

Eurasian Journal of Applied Biotechnology Pub Date : 2020-06-01 DOI: 10.11134/BTP.2.2020.1

D. Begimbetova, D. Baiskhanova, B. Matkarimov, Z. Shulgau

{"title":"In Vitro and In Vivo Effects of Radio- Frequency Electromagnetic Fields","authors":"D. Begimbetova, D. Baiskhanova, B. Matkarimov, Z. Shulgau","doi":"10.11134/BTP.2.2020.1","DOIUrl":"https://doi.org/10.11134/BTP.2.2020.1","url":null,"abstract":"In recent years, there has been a massive distribution of mobile phones and Wi-Fi networks. In this regard, concerns about their potential effects on living organisms, including humans, are increasing. Despite earlier assumptions about the absence of harmful effects of short-term exposure to radio frequency electromagnetic fields emitted by mobile phones and Wi-Fi networks, there is now increasing evidence of the potentially harmful effects of electromagnetic fields on the human body through the induction of oxidative stress and damage to DNA structure. It is assumed that prolonged exposure to electromagnetic fields can cause the development of various pathological conditions in the human body, including oncological diseases, impaired cognitive functions and sleep, etc. \u0000This review analyzes and summarizes existing studies indicating various negative effects of radio frequency electromagnetic fields on human and animal cells. Since there is a large amount of conflicting data on the effect of electromagnetic fields on the human body, only further research can provide an answer to the possible negative effects of mobile phones and Wi-Fi networks usage.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"79 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113960064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1