Eurasian Journal of Applied Biotechnology最新文献_第9页

OPTIMIZATION OF PROCESS PARAMETERS FOR CULTIVATION OF BACTERIOPHAGES PROMISING FOR BIOLOGICAL CONTROL OF PHYTOPATHOGENIC BACTERIA OF GENUS PSEUDOMONAS 假单胞菌属植物致病菌生物防治噬菌体培养工艺参数的优化

Eurasian Journal of Applied Biotechnology Pub Date : 2021-09-24 DOI: 10.11134/btp.3.2021.3

T. A. Pilipchuk, A.D. Gerasimovich, I. N. Ananyeva, E. Kolomiets

引用次数: 0

EXPRESSION OF THE STAPHYLOCOCCUS AUREUS LUKE GENE IN THE ESCHERICHIA COLI BL21(DE3) STRAIN 金黄色葡萄球菌Luke基因在大肠杆菌bl21 (de3)中的表达

Eurasian Journal of Applied Biotechnology Pub Date : 2021-09-24 DOI: 10.11134/btp.3.2021.2

G. Abitayeva, D. Bulanin, E. Marchenko, L. Vangelista

{"title":"EXPRESSION OF THE STAPHYLOCOCCUS AUREUS LUKE GENE IN THE ESCHERICHIA COLI BL21(DE3) STRAIN","authors":"G. Abitayeva, D. Bulanin, E. Marchenko, L. Vangelista","doi":"10.11134/btp.3.2021.2","DOIUrl":"https://doi.org/10.11134/btp.3.2021.2","url":null,"abstract":"Two-component leukotoxins are important virulence factors for Staphylococcus aureus. Despite efforts made to study S. aureus leukotoxins, the direct mechanism of action of these toxins during infection has not been determined. However, the observation that deletion of LukED significantly attenuates highly virulent S. aureus strains supports the hypothesis that selective inhibition of LukE / D may be useful in the development of new aspects of S. aureus infection control. For this purpose, this work was carried out to test the expression and obtain a recombinant form of the LukE protein in E.coli cells. The LukE gene was cloned into the pET28-c (+) / GFP vector containing the gfp gene. Two fused genes carrying a hexahistidine tag were expressed in cells of the E. coli BL21(DE3) strain. It was found that the 6His-GFP-LucE protein aggregates in inclusion bodies. 6His-GFP-LucE was washed out of inclusion bodies with high molar urea. The 6His-GFP-LucE protein was purified by metal affinity chromatography. Research results can be applied to obtain recombinant protein including strategies for inhibition of toxin activity.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"356 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132793662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MICROCLONAL PROPAGATION OF SPIRAEA JAPONICA FOR LANDSCAPING 园林用绣线菊微克隆繁殖研究

Eurasian Journal of Applied Biotechnology Pub Date : 2021-09-24 DOI: 10.11134/btp.3.2021.4

V. Karimova, G. Magzumova, A. Yessimseitova, A. Kakimzhanova

引用次数: 0

INFLUENCE OF ABIOTIC STRESS FACTORS ON THE HYDRATION OF PLANT TISSUES OF SEDUM HYBRIDUM L. (AIZOPSIS HYBRIDA (L.) GRULICH) 非生物胁迫因素对秋葵(sedum hybridum l .)植株组织水化的影响GRULICH)

Eurasian Journal of Applied Biotechnology Pub Date : 2021-09-24 DOI: 10.11134/btp.3.2021.5

N. Terletskaya, T. N. Kobylina, Zh.A. Kenzhebayeva

引用次数: 0

Gut Microbiota Modulation In Diabetic Patients 糖尿病患者肠道菌群调节

Eurasian Journal of Applied Biotechnology Pub Date : 2021-06-24 DOI: 10.11134/btp.2.2021.4

S. Kozhakhmetov, N. Mukhanbetzhanov, А.R Kushugulova

引用次数: 0

The Development And Characterization Of Heparin-Conjugated Fibrin Hydrogel With Incapsulated Mesenchimal Stem Cells And Growth Factors 包埋间充质干细胞和生长因子的肝素偶联纤维蛋白水凝胶的制备与表征

Eurasian Journal of Applied Biotechnology Pub Date : 2021-06-24 DOI: 10.11134/btp.2.2021.3

M. Sarsenova, A. Issabekova, M. Karzhauov, G. Kudaibergen, M. Zhunussova, V. Ogay

引用次数: 0

Study of proteolytic and keratinolytic enzymes of bacillus sp. A5.3 芽孢杆菌A5.3蛋白水解酶和角蛋白水解酶的研究

Eurasian Journal of Applied Biotechnology Pub Date : 2021-06-24 DOI: 10.11134/btp.2.2021.6

S. Aktaeva, M. Saparbayev

{"title":"Study of proteolytic and keratinolytic enzymes of bacillus sp. A5.3","authors":"S. Aktaeva, M. Saparbayev","doi":"10.11134/btp.2.2021.6","DOIUrl":"https://doi.org/10.11134/btp.2.2021.6","url":null,"abstract":"Feathers, as a major part of the waste from the industrial poultry industry, require a special approach for disposal and, at the same time, are of interest as a source of feed protein. Feather biomass consists of 90% β-keratin, hydrolysates of which can be a valuable source of pepton, but feather keratin is highly resistant to most proteolytic enzymes. For hydrolysis, therefore, keratin must be subjected to special treatment, the purpose of which is to break down the compact structure of the keratin molecule to produce polypeptides, peptides and single amino acids. For enzymatic hydrolysis of keratin, proteases with keratinase activity are used, capable of cleaving keratin disulfide bonds. A strain of Bacillus sp. A5.3 was isolated from feather waste sites and showed high proteolytic and keratinolytic activity. The strain is able to grow on minimal feather medium and has caseinolytic, collagenase and β-keratinolytic activity. The secretory proteome of the strain was studied using nano HPLC/Q-TOF-MS. As a result, 154 proteins were identified, 13 of which are proteases and peptidases. The genes for 3 proteases and peptidases clpY, clpX and ytjP were amplified from the genomic DNA of Bacillus sp. A5.3, sequenced, and the nucleotide sequence of the genes was deposited in the GenBank database. A study of a strain of Bacillus sp. A5.3 showed that the strain is capable of effective feather degradation and promising as a producer of proteolytic and keratinolytic enzymes.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129139943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Galactosidases Of Strain Bifidobacterium Longum Bim В-813d With Transglycosylating Activity 长双歧杆菌Bim В-813d具有转糖基化活性的半乳糖苷酶

Eurasian Journal of Applied Biotechnology Pub Date : 2021-06-24 DOI: 10.11134/btp.2.2021.5

A. N. Morozova, N. Golovnyova, N. Е. Ryabaya, M. E. Safonova

引用次数: 1

Study Of The Influence Of Hypoxia On The Formation Of Erythrocytes From Induced Pluripotent Human Stem Cells 缺氧对诱导多能人干细胞形成红细胞影响的研究

Eurasian Journal of Applied Biotechnology Pub Date : 2021-06-24 DOI: 10.11134/btp.2.2021.2

А.Y Sekenova, V. Kumasheva, Sh.Е. Muhlis, V.B. Оgay

{"title":"Study Of The Influence Of Hypoxia On The Formation Of Erythrocytes From Induced Pluripotent Human Stem Cells","authors":"А.Y Sekenova, V. Kumasheva, Sh.Е. Muhlis, V.B. Оgay","doi":"10.11134/btp.2.2021.2","DOIUrl":"https://doi.org/10.11134/btp.2.2021.2","url":null,"abstract":"The production of erythrocytes from human hematopoietic stem cells (HSC) is considered one of the solutions to the deficiency of donor blood in transfusion medicine. The disadvantage of using HSC is the difficulty in multiplying in the required amount for use in transfusion. In this regard, pluripotent stem cells that can be multiplied in sufficient quantity may become a source of functional erythrocytes. The aim of this work was to study the effects of hypoxia on the formation of erythrocytes from human induced pluripotent stem cells (iPSCs) in ex vivo conditions. For the obtaining the erythroid bodies (EB), human iPSCs were cultured in induction medium containing human plasma and SCF, VEGF, BMP-4, TPO, EPO, interleukins IL-3 and IL-6 (20 days). EB treated with collagenase and cultured in a 96-well suspension culture plate under normoxia (21% O2) and hypoxia (2% O2) conditions in IMDM medium containing 20% human plasma, SCF, IL-3, Epo, insulin and heparin. Erythroid maturation was evaluated by Giemsastaining and flow cytometry analysis ofCD235α. As a result of PSC differentiation, EBs with spherical morphology and sizes from 120 to 300 µm were obtained. Analysis for erythroid maturation showed that the number of mature erythrocytes with expression of CD235α after hypoxia averaged 56%, and after normoxia 19.3% of the total number of analyzed cells. The size of erythrocytes formed from iPSCs averaged 7 μm, which corresponds to the mature form of this type of cells. Thus, the obtained results of the study indicate that hypoxia plays a significant role in erythropoiesis, increasing the level of erythrocyte formation from human iPSCs.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128320121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytoecdysteroids: Chemical Structure And Biological Activity 植物甾体:化学结构和生物活性

Eurasian Journal of Applied Biotechnology Pub Date : 2021-06-24 DOI: 10.11134/btp.2.2021.1

Zh. S. Akhmetkarimova, B. S. Temirgaziev

引用次数: 0