Eurasian Journal of Applied Biotechnology最新文献

Cloning of cDNA-gene of Arabidopsis thaliana ribosomal protein S6, its expression in Escherichia coli and purification of AtRPS6B1 recombinant protein 拟南芥核糖体蛋白 S6 cDNA 基因的克隆、在大肠杆菌中的表达以及 AtRPS6B1 重组蛋白的纯化

Eurasian Journal of Applied Biotechnology Pub Date : 2024-05-02 DOI: 10.11134/btp.1.2024.7

L. Nadirova, D. Beisenov, G. Stanbekova, A. Zhigailov, L.A. Ryabova, B. Iskakov

{"title":"Cloning of cDNA-gene of Arabidopsis thaliana ribosomal protein S6, its expression in Escherichia coli and purification of AtRPS6B1 recombinant protein","authors":"L. Nadirova, D. Beisenov, G. Stanbekova, A. Zhigailov, L.A. Ryabova, B. Iskakov","doi":"10.11134/btp.1.2024.7","DOIUrl":"https://doi.org/10.11134/btp.1.2024.7","url":null,"abstract":"Ribosomal protein S6 is a component of the 40S small ribosomal subunit and plays an important role in protein biosynthesis. Its main function is related to the regulation of mechanisms that control cell growth and division. The RPS6 can be phosphorylated on defined serine and threonine residues by kinases such as S6K1 and S6K2, which in turn are activated by signaling pathways associated with the activation of the mTORC1 molecular complex. The phosphorylation process of protein RPS6 plays a key role in the regulation of cell growth and protein synthesis. Activated RPS6 protein influences translation initiation, which means start the process of protein synthesis on the ribosome. Thus, RPS6 is associated with the regulation of cell size and their ability to divide. In addition, the RPS6 protein can be associated with other proteins and participate in various molecular interactions, which may vary depending on the context of cellular activity. In this study, cloning and site-directed cDNA mutagenesis of the second isoform of the AtRPS6 protein (AtRPS6B) was performed. Further, the obtained phosphomimetic and nonphosphorylated forms of this protein were expressed in E.coli ArcticExpress (DE3) cells, the proteins were purified by metal chelate chromatography (IMAC) and the presence and purity of the obtained proteins was confirmed by immunoblotting","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"37 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141019844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRODUCTION OF RECOMBINANT MILK-CONVERTING ENZYME IN YEAST PICHIA PASTORIS 在皮氏酵母中生产重组牛奶转化酶

Eurasian Journal of Applied Biotechnology Pub Date : 2024-04-04 DOI: 10.11134/btp.1.2024.6

Z. Akishev, M. Auyez, K. Baltin, A. Tursunbekova, B. Khassenov

引用次数: 0

SUPPRESSOR CELLS (MDSC) REGULATE THE T-CELL RESPONSE IN OLD MICE IMMUNIZED WITH THE WHOLE-VIRION SARS-CoV-2 VACCINE 抑制细胞（MDSC）调节接受全病毒 SARS-CoV-2 疫苗免疫的老龄小鼠的 T 细胞反应

Eurasian Journal of Applied Biotechnology Pub Date : 2024-04-04 DOI: 10.11134/btp.1.2024.5

Y. Perfilyeva, N. Abdolla, A. Kali, R. Tleulieva, D. Aben, Y. Abdusattarova, A. Lushova, Ye. Ostapchuk, B. Karalnik, N. Belyaev

{"title":"SUPPRESSOR CELLS (MDSC) REGULATE THE T-CELL RESPONSE IN OLD MICE IMMUNIZED WITH THE WHOLE-VIRION SARS-CoV-2 VACCINE","authors":"Y. Perfilyeva, N. Abdolla, A. Kali, R. Tleulieva, D. Aben, Y. Abdusattarova, A. Lushova, Ye. Ostapchuk, B. Karalnik, N. Belyaev","doi":"10.11134/btp.1.2024.5","DOIUrl":"https://doi.org/10.11134/btp.1.2024.5","url":null,"abstract":"Myeloid-derived suppressor cells (MDSCs) are an immunosuppressive population involved in pathological processes associated with chronic inflammation. In recent years, the role of these cells in the regulation of a number of non-pathological processes, in particular, in the development of vaccine-induced immune response, has been actively discussed. This work was aimed at investigating the role of MDSC in the development of an immune response in old mice immunized with an inactivated whole virion SARS-CoV-2. Immunization of old animals induced an increase in the relative content of B cells and the production of specific antibodies to S1 and N proteins of SARS-CoV-2 in titers comparable to those of young animals. At the same time, elevated MDSC levels in aged animals negatively correlated with the level of CD8+ T cells. Splenic MDSCs in old animals were characterized by increased production of reactive oxygen species compared to young animals. In vitro experiments showed that MDSC depletion increased antigen-specific production of IFNγ by CD4+ T cells. The data obtained suggest a negative role for MDSC in regulating the T-cell vaccine-induced response to inactivated SARS-CoV-2 and encourage further studies in this direction, which may be a key to developing approaches to increase the efficacy of vaccines against SARS-CoV-2 in at-risk groups.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140742486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SEROLOGICAL SURVEY OF LYME BORRELIOSIS IN THE SOUTH REGION OF KAZAKHSTAN 哈萨克斯坦南部地区莱姆包虫病血清学调查

Eurasian Journal of Applied Biotechnology Pub Date : 2024-04-04 DOI: 10.11134/btp.1.2024.4

A. Kuligin, A. Lushova, S. Kan, A. Bissenbay, Y. Perfilyeva, N. Abdolla, A. Dmitrovskiy, Y. Skiba, N. Ospanbekova, T. Davlyatshin, G. Abuova, M. Bayakhmetova, A. Adil, Ye. Ostapchuk

{"title":"SEROLOGICAL SURVEY OF LYME BORRELIOSIS IN THE SOUTH REGION OF KAZAKHSTAN","authors":"A. Kuligin, A. Lushova, S. Kan, A. Bissenbay, Y. Perfilyeva, N. Abdolla, A. Dmitrovskiy, Y. Skiba, N. Ospanbekova, T. Davlyatshin, G. Abuova, M. Bayakhmetova, A. Adil, Ye. Ostapchuk","doi":"10.11134/btp.1.2024.4","DOIUrl":"https://doi.org/10.11134/btp.1.2024.4","url":null,"abstract":"Lyme borreliosis (LB) is an infectious disease caused by spirochetes of the Borrelia burgdorferi sensu lato complex. Transmission occurs through the bites of hard ticks within the genus Ixodes. Cases of fever of unknown origin are recorded in southern Kazakhstan, however, the prevalence of LB in this area remains unstudied. Almaty oblast is recognized as an endemic area for LB. Ixodid tick vectors also inhabit Jambyl oblast, where antibodies to LB agent were previously detected among residents, while it is classified as non-endemic. Turkistan oblast is also considered non-endemic for LB. The purpose of this study was to investigate the seroprevalence of IgG antibodies to B. burgdorferi s.l. among apparently healthy residents of Almaty city (Almaty oblast), Taraz city (Jambyl oblast), and Shymkent city (Turkistan oblast). A total of 465 human serum samples were collected from March 2022 to September 2023. Using the ELISA method, we detected the presence of IgG antibodies to the LB agent in 5.4% of the entire study group (24/465, 95% CI: 3.3-7.6%), and specifically in 9.3% of residents of Almaty city (11/118, 95% CI: 4.7-16.1%, p=0.03), 4.5% – Taraz city (11/242, 95% CI: 2.3-8.0%) and 1.9% – Shymkent city (2/105, 95% CI: 0.2-6.7%). The results of the study confirm the seroprevalence of IgG antibodies to B. burgdorferi s.l. among the population of Almaty city and Jambyl oblast, and also reveal seropositive samples in residents of Turkistan oblast, suggesting possible infection during travel to endemic regions. It is important to continue LB surveillance in endemic and emerging regions, as well as to raise awareness of the infection among healthcare workers and the general public in non-endemic areas.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"21 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140742003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EVALUATION OF NORMALIZED DIFFERENCE VEGETATION INDEX IN WILD APPLE POPULATIONS GROWING IN SOUTHEAST MOUNTAIN REGIONS OF KAZAKHSTAN 评估哈萨克斯坦东南山区野生苹果种群的归一化差异植被指数

Eurasian Journal of Applied Biotechnology Pub Date : 2024-04-03 DOI: 10.11134/btp.1.2024.2

A. Taskuzhina, M. Khusnitdinova, N. Kerimbek, A. Kapytina, A. Pozharskiy, R. Moisseyev, D. Gritsenko

引用次数: 0

MOLECULAR DETECTION OF FUSOBACTERIUM NUCLEATUM IN IN KAZAKHSTANY PATIENTS WITH COLORECTAL CANCER 分子检测哈萨克斯坦结直肠癌患者体内的核分枝杆菌

Eurasian Journal of Applied Biotechnology Pub Date : 2024-04-03 DOI: 10.11134/btp.1.2024.3

B. Kurentay, A.D. Gusmaulemova, T.A. Utupov, D.N. Auganova, P. Tarlykov, S.S. Khamzina, M. Mamlin, A.M. Kozhakhmetov, S. Shalekenov, G. Kulmambetova

引用次数: 0

Biobanks as a way to conserve biodiversity in the context of the continuing decline in the species diversity of wild flora and fauna 在野生动植物物种多样性持续减少的情况下，生物库作为保护生物多样性的一种方式

Eurasian Journal of Applied Biotechnology Pub Date : 2024-04-01 DOI: 10.11134/btp.1.2024.1

A.K. Dairov, K. Mukanov, O. Khapilina, Sh.A. Manabayeva, A.A. Kakimzhanova, A.E. Khassenova, S. Kubentayev, E. Zholdybayeva, B. Khassenov, A. Shevtsov, P. Tarlykov, K. N. Mukantayev, Yerlan Ramankulov

{"title":"Biobanks as a way to conserve biodiversity in the context of the continuing decline in the species diversity of wild flora and fauna","authors":"A.K. Dairov, K. Mukanov, O. Khapilina, Sh.A. Manabayeva, A.A. Kakimzhanova, A.E. Khassenova, S. Kubentayev, E. Zholdybayeva, B. Khassenov, A. Shevtsov, P. Tarlykov, K. N. Mukantayev, Yerlan Ramankulov","doi":"10.11134/btp.1.2024.1","DOIUrl":"https://doi.org/10.11134/btp.1.2024.1","url":null,"abstract":"To date, human actions have significantly changed 75% of the Earth's surface. This has resulted in an unprecedented increase in the rate of biodiversity loss in human history. According to the International Union for Conservation of Nature and Natural Resources, over the past 500 years, as a result of direct or indirect influence of the anthropogenic factor, 774 animal species and 123 plant species have become extinct. To prevent further loss of biodiversity, in the context of the ongoing «crisis of biodiversity», all over the world, including Kazakhstan, there is a need for reliable conservation of the available genetic resources of plants and animals, primarily rare and endangered species. In this regard, specialized biobanks of organized collections of living biomaterials are successfully used worldwide for the conservation and restoration of populations of rare and endangered species of flora and fauna. The article presents information about some of the largest biobanks and their importance in solving this global problem. The experience of scientific centers of Kazakhstan in the field of biobanking of biomaterials of wild flora and fauna, including rare and endangered species, is given, and the results of the National Center for Biotechnology in this area are summarized.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"228 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140769178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GENETIC DIVERSITY AND ASSOCIATION ANALYSIS OF SALT TOLERANCE IN ASIATIC COTTON (GOSSYPIUM ARBOREUM) WITH MOLECULAR MARKERS 利用分子标记对亚洲棉花（gossypium arboreum）耐盐性的遗传多样性和关联分析

Eurasian Journal of Applied Biotechnology Pub Date : 2024-01-07 DOI: 10.11134/btp.4.2023.8

Tussipkan Dilnur, Pan Zhaoe, Wenfang Gong, Panhong Dai, Manabayeva Shuga, X. Du

{"title":"GENETIC DIVERSITY AND ASSOCIATION ANALYSIS OF SALT TOLERANCE IN ASIATIC COTTON (GOSSYPIUM ARBOREUM) WITH MOLECULAR MARKERS","authors":"Tussipkan Dilnur, Pan Zhaoe, Wenfang Gong, Panhong Dai, Manabayeva Shuga, X. Du","doi":"10.11134/btp.4.2023.8","DOIUrl":"https://doi.org/10.11134/btp.4.2023.8","url":null,"abstract":"Gossypium arboreum possesses favorable characteristics that are valuable for developing superior cotton cultivars. This study was carried out to analyze genetic diversity and identify marker-trait association analysis related to salt tolerance using SSR markers for 215 accessions of G. arboreum. The salt tolerance-related traits like germination rate, fresh weight, stem length, water content, chlorophyll content, electric conductivity, and MDA of the accessions were identified using 150 mM salt for 7 days of seedling growth. \u0000According to the comprehensive index of salt tolerance, 215 accessions were categorized mainly into four groups. Twenty four accessions were classified as highly tolerant to salt treatment (>2.5). The natural population was classified into 3 main groups by phylogenetic analysis. The classifications of phylogenetic analysis were largely congruent with the breeding history and ecological region. \u0000Twenty-two strong marker-trait associations were obtained with strict significant P value i.e. P< 0.01 and four makers including NAU1023, NAU1099, JESPR222, and NAU2783 were significantly related to salt tolerance. The marker NAU2783 was highest associated (P= 1.98E-12) with relative electric conductivity, and with the highest phenotype variation of 20.87%. Some markers are significantly associated with more than two traits. MUSS020 was significantly (P<0.01) associated with relative fresh weight, and relative MDA, NAU1375 was associated with relative fresh weight and relative stem length, while NAU3468 was significantly associated with relative fresh weight, relative germination rate, and relative water content. The strong marker-trait association results might provide insights for marker-assistant selecting salt-tolerant varieties and will be useful for future cotton breeding programs.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139448534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PHENOTYPIC AND GENETIC DIVERSITY OF ENTEROVIRUSES IN THE REPUBLIC OF BELARUS, 2003-2022 白俄罗斯共和国肠道病毒的表型和遗传多样性，2003-2022 年

Eurasian Journal of Applied Biotechnology Pub Date : 2023-12-28 DOI: 10.11134/btp.4.2023.7

N. Paklonskaya, T. Amvrosieva, Z. F. Bogush, Y. Shilova, Y. Koltunova, A.M. Dashkevich, V.V. Zapolskaya, E. Kishkurno

{"title":"PHENOTYPIC AND GENETIC DIVERSITY OF ENTEROVIRUSES IN THE REPUBLIC OF BELARUS, 2003-2022","authors":"N. Paklonskaya, T. Amvrosieva, Z. F. Bogush, Y. Shilova, Y. Koltunova, A.M. Dashkevich, V.V. Zapolskaya, E. Kishkurno","doi":"10.11134/btp.4.2023.7","DOIUrl":"https://doi.org/10.11134/btp.4.2023.7","url":null,"abstract":"Enteroviruses (EV) are widespread viral agents that regularly cause epidemic outbreaks of diseases that are characterized by a variety of clinical manifestations. The aim of this work was to study the phenotypic and genotypic diversity of non-poliomyelitis EVs that circulated from 2003 to 2022 in our country during epidemic upsurges and decreases of enteroviral morbidity. It was established that during this period there was a circulation of 44 different types of EVs belonging to the species Enterovirus B (97.8%), Enterovirus A (1.88%), Enterovirus C (0.9%), Enterovirus D (0.4 %). Among Enterovirus B viruses, Coxsackievirus B5, ECHO 30, and ECHO11 prevailed. During the years of epidemic rises of morbidity, the ECHO 30, ECHO 9 and ECHO 11 viruses were significantly more often identified (p< 0.001), while in the years of decreases Coxsackievirus B1, B2, B3 and B4 were detected more often (p< 0.001). ECHO 6 and Coxsackievirus B5 viruses were registered both during periods of epidemic ups and downs and were characterized by the maximum intratype genetic diversity, significantly greater than ECHO30, ECHO6 and ECHO 11 (p< 0.05). During the observation period, 9 genetic variants of ECHO 30, 6 – of ECHO 9, 3 – of ECHO 11, 6 – of ECHO 6 and 13 – of Coxsackievirus B5 were identified. An analysis of the genetic diversity of ECHO 30, 9, 11, 6, Coxsackie B5 viruses showed that the upsurges of enteroviral morbidity was accompanied by the maximum number of detected genetic variants and some of them were new to the country's population. The data obtained indicate the multifactorial nature of the formation of the epidemic process and a significant contribution to its formation of the genetic diversity of pathogens.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"32 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139152092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

INFLUENCE OF LOW POSITIVE TEMPERATURES ON ANATOMICAL CHARACTERISTICS JUGLANS REGIA L. IN VITRO MICROCLONES 低温对离体微型克隆的解剖学特征的影响

Eurasian Journal of Applied Biotechnology Pub Date : 2023-12-28 DOI: 10.11134/btp.4.2023.6

N.V. Terletskaya, M. Yerbay, E.A. Shadenova, N. K. Korbozova, I. Nazarova, Ju.A. Litvinenko, N. O. Kudrina

引用次数: 0