Isolation and Identification of New Strains of Lactic Bacteria from The Microbiom of Young Carp Fish

Abstract

Isolation and screening of new strains of lactic acid bacteria from the gut microbiome of carp and carp fingerlings were carried out. Morphological and cultural properties of isolates were studied. All isolates revealed high antagonistic activity against such opportunistic bacteria as Shewanella ximenensis AU 2R-1 B-RKM 0724, Pseudomonas taiwanensis CB 2R-1 B-RKM 0726, Pseudomonas aeruginosa G13 B-RKM 0427, Aeromonas punctata G30 B-RKM 0287, Staphylococcus aureus В-RKM 0470, Escherichia coli В-RKM 0447. Based on the analysis of the nucleotide sequences of the 16S rRNA gene fragment, 6 most active LAB cultures were identified, which belong to representatives of the genera Pediococcus, Lactobacillus and Leuconostoc. The procedure of depositing all strains in the Central Museum of Microorganisms of the Republican Collection of Microorganisms was carried out.