Eurasian Journal of Applied Biotechnology最新文献_第4页

POSSIBILITIES OF USING MEDICINES AND BIOLOGICALLY ACTIVE SUBSTANCES AS CORRECTIVES FOR THE FORMATION OF PULMONARY FIBROSIS DURING SARS-COV-2 INFECTION AND IN THE POST-COVID PERIOD 在sars-cov-2感染期间和covid - 19后时期使用药物和生物活性物质作为肺纤维化形成纠正剂的可能性

Eurasian Journal of Applied Biotechnology Pub Date : 2023-04-13 DOI: 10.11134/btp.1.2023.1

Z. Shulgau, Y. Kamyshanskiy, A. Gulyayev

引用次数: 0

THE OBTAINING OF THE RECOMBINANT CAMEL CHYMOSIN BY SUBMERGE FERMENTATION IN THE PILOT BIOREACTOR 在中试生物反应器中浸发酵法获得重组骆驼凝乳酶

Eurasian Journal of Applied Biotechnology Pub Date : 2023-04-13 DOI: 10.11134/btp.1.2023.4

Z. Akishev, A. Abdullayeva, A. Mussakhmetov, A. Bekbayeva, A. Tursunbekova, B. Kalemshariv, T. Tultabayeva, B. Khassenov

{"title":"THE OBTAINING OF THE RECOMBINANT CAMEL CHYMOSIN BY SUBMERGE FERMENTATION IN THE PILOT BIOREACTOR","authors":"Z. Akishev, A. Abdullayeva, A. Mussakhmetov, A. Bekbayeva, A. Tursunbekova, B. Kalemshariv, T. Tultabayeva, B. Khassenov","doi":"10.11134/btp.1.2023.4","DOIUrl":"https://doi.org/10.11134/btp.1.2023.4","url":null,"abstract":"In the cheese making industry, chymosin is used as a milk-clotting enzyme. With its high specific activity against κ-casein, chymosin better than other proteolytic enzymes. Bactrian camel chymosin has a milk-clotting activity higher than calf chymosin. A scheme for obtaining a milk-clotting preparation based on recombinant camel chymosin is proposed. Submerge fermentation of recombinant yeast Pichia pastoris was carried out in a 50-liter bioreactor and recombinant camel chymosin was obtained. The activity of chymosin in the yeast culture was 174.5 U/mL. Chymosin was concentrated 5.6-fold by cross-flow ultrafiltration with 10 kDa cut-off membrane, and chymosin was purified by ion exchange chromatography. The activity of purified chymosin was 4700 U/mL. By sublimation drying with casein peptone, the powder chymosin was obtained with an activity of 36,000 U/g. The proposed scheme for obtaining a milk-clotting drug based on recombinant camel chymosin using submerge fermentation of recombinant yeast has the prospect of being used at biotechnological enterprises. ","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121271579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

OPTIMIZATION OF CULTIVATION CONDITIONS FOR RECOMBINANT INFLUENZA VIRUSES EXPRESSING IMMUNODOMINANT M. BOVIS PROTEINS 表达免疫优势牛分枝杆菌蛋白的重组流感病毒培养条件优化

Eurasian Journal of Applied Biotechnology Pub Date : 2023-04-13 DOI: 10.11134/btp.1.2023.7

E. Voronina, Z. Abay, A. Nurpeisova, B. Abdrakhmanova, K. Jekebekov, S. Sadikalieva, Y. Bulatov, N. Kozhabergenov, K. Zakarya, K. Shorayeva

{"title":"OPTIMIZATION OF CULTIVATION CONDITIONS FOR RECOMBINANT INFLUENZA VIRUSES EXPRESSING IMMUNODOMINANT M. BOVIS PROTEINS","authors":"E. Voronina, Z. Abay, A. Nurpeisova, B. Abdrakhmanova, K. Jekebekov, S. Sadikalieva, Y. Bulatov, N. Kozhabergenov, K. Zakarya, K. Shorayeva","doi":"10.11134/btp.1.2023.7","DOIUrl":"https://doi.org/10.11134/btp.1.2023.7","url":null,"abstract":"The use of recombinant viral vectors is one of the promising approaches to the creation of a new generation of vaccines against bovine tuberculosis. For the prevention of tuberculosis in cattle, two reassortant recombinant strains of influenza A virus expressing mycobacterial proteins ESAT-6 and TB-10.4 were constructed based on the method of reverse genetics. As a result of optimization of virus cultivation conditions, the cytopathic effect of the virus was recorded on the Vero cell culture. It has been established that the maximum accumulation of viruses is noted when cultivating in a Vero cell culture using a serum-free DMEM medium at a temperature of (37 ± 0.5) ºС, with access to 5% carbon dioxide. The peak of the biological activity of the recombinant strains for both samples reached at 72 hours of the study and lasted for 96 hours. The biological activity of FLU NS_ESAT 6 was 7.50 ± 0.08 lg TCD50/cm3 , and for FLU NS_TB10.4 was 7,75 ± 0,08 lg TCD50/cm3. The results obtained are the basis for the development of viral biomass for work on the creation of an experimental series of vaccines against bovine tuberculosis.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126559408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BIOTECHNOLOGICAL APPROACHES TO THE CREATION OF NEW FERMENTED DAIRY PRODUCTS 生物技术方法创造新的发酵乳制品

Eurasian Journal of Applied Biotechnology Pub Date : 2023-02-22 DOI: 10.11134/btp.4.2022.16

S. Nadirova, Y. Sinyavskiy

{"title":"BIOTECHNOLOGICAL APPROACHES TO THE CREATION OF NEW FERMENTED DAIRY PRODUCTS","authors":"S. Nadirova, Y. Sinyavskiy","doi":"10.11134/btp.4.2022.16","DOIUrl":"https://doi.org/10.11134/btp.4.2022.16","url":null,"abstract":"The industrial production of balanced and healthy food has always been one of the most important tasks facing humanity. To solve this problem, research is relevant in the field of creating functional milk-based synbiotic products, which, along with traditional lactic acid microorganisms, include strains of probiotic bacteria with a proven specific positive effect on the macroorganism, and prebiotic substances that increase the selectivity of the benefits of the \"useful\" microflora of the gastrointestinal tract-intestinal tract and its biological activity.Along with lactic acid bacteria used as starters in fermented milk products, there is a population of predominantly adventitious heterofermentative lactobacilli that can have a positive effect on dairy products.This article describes the use of facultatively and obligately heterofermentative species such as Lactobacillus bulgaricus, Streptococcus thermophilus and Lactobacillus fermentum 14. The general characteristics of these species are described, as well as the desired properties (probiotic activity and taste formation) that affect the characteristics of dairy products.The general characteristics of these species are described along with the desired (probiotic activity and taste formation) properties affecting the characteristics of dairy products.Samples of yogurt based on probiotic starter cultures enriched with plant fillers were studied. The technological process of production was carried out in the traditional way. The dynamics of acid accumulation was studied, titratable and active acidity were determined. As a result of the study, it was revealed that the combination of fermented milk products with plant fillers slightly accelerates the fermentation process.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"19 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120921720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

WHOLE GENOME SEQUENCING AND GENOTYPING PROTOCOL OF RABIES VIRUS ISOLATES 狂犬病毒分离株全基因组测序及基因分型方案

Eurasian Journal of Applied Biotechnology Pub Date : 2023-01-04 DOI: 10.11134/btp.4.2022.15

G. Yessembekova, A. Abenova, A. Amirgazin, T. Karibayev, A. Shevtsov, S. Abdrakhmanov

{"title":"WHOLE GENOME SEQUENCING AND GENOTYPING PROTOCOL OF RABIES VIRUS ISOLATES","authors":"G. Yessembekova, A. Abenova, A. Amirgazin, T. Karibayev, A. Shevtsov, S. Abdrakhmanov","doi":"10.11134/btp.4.2022.15","DOIUrl":"https://doi.org/10.11134/btp.4.2022.15","url":null,"abstract":"This study describes the developed protocol for whole genome sequencing of clinical Rabies virus RNA samples on the Illumina MiSeq platform. Previously, genotyping of the rabies virus circulating in Kazakhstan was based on the nucleoprotein gene sequence (N gene). The protocol describes the methods of sample preparation, whole genome sequencing and genotyping currently used for epidemiological analysis not only of Rabies virus, but also for other viral pathogens such as SARS-CoV-2, Influenza and others. The aim of the work was to develop and test a protocol for whole genome sequencing and genetyping of rabies virus isolates for further use in epidemiological monitoring. As a result of the research, whole genome sequences of 31 isolates of Rabies virus isolated from various infected animals and regions of Kazakhstan were obtained for the first time. All the isolates belong to the major steppe-type clade – Cosmopolitan (30 isolates belong to the minor clade CA1, while 1 isolate belongs to an unassigned minor clade). 2 local outbreaks and 6 separated rabies virus lineages were identified. Estimated dates of divergence from a common ancestor were calculated for both the Cosmopolitan clade and the minor clade CA1.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128735860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CHANGES IN DIFFERENTIAL GENE EXPRESSION PATTERN IN FLY HEADS AS THE RESPONSE TO AN EXPERIMENTALLY EVOLVED X CHROMOSOME 果蝇头部差异基因表达模式的变化是对实验进化的x染色体的反应

Eurasian Journal of Applied Biotechnology Pub Date : 2022-12-06 DOI: 10.11134/btp.4.2022.14

Y. Manat

{"title":"CHANGES IN DIFFERENTIAL GENE EXPRESSION PATTERN IN FLY HEADS AS THE RESPONSE TO AN EXPERIMENTALLY EVOLVED X CHROMOSOME","authors":"Y. Manat","doi":"10.11134/btp.4.2022.14","DOIUrl":"https://doi.org/10.11134/btp.4.2022.14","url":null,"abstract":"Sexually antagonistic (SA) mutations that increase fitness in one sex and decrease it in the other sex (also known as intralocus sexual conflict) are central to the sexual antagonism hypothesis of sex chromosome evolution. It was suggested long ago (by Fisher, in 1931) that tight linkage to a sex-determining locus facilitates the accumulation of SA mutations even when their detrimental effect in one sex exceeds its benefit in the other. Due to the difficulties in detecting SA alleles directly, we know very little about the nature of X-linked SA mutations and their role in the evolution of differential gene expression between sexes. To investigate how X-linked SA mutations affect the transcriptome profile in both sexes, we analyzed changes in genome-wide gene expression pattern in Drosophila melanogaster head tissue that had undergone 95 generations of a female-limited X-chromosome experimental evolution. We found an evidence of change in gene expression towards to the female optimum, which we interpret as the result of resolution of sexual conflict over X-linked SA mutations. In addition, we also found a potential unknown gene expression effect of the balancer chromosome on gene expression (that was necessary to use to limit the inheritance of target X chromosome to the female line). All these findings indicate that a highly polymorphic nature of the X chromosome with an important role in genome-wide gene expression profile.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"109 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116160422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the KRAS mutations in colorectal and pancreatic cancer patients 结直肠癌和胰腺癌患者KRAS突变的评估

Eurasian Journal of Applied Biotechnology Pub Date : 2022-11-13 DOI: 10.11134/btp.4.2022.13

D. Samatkyzy, S. Rakhimova, D. Begimbetova, A. Kukanova, F. Fazyl, I. Stefanov, O. Pirozhenko, A. Gabdulkayum, U. Kozhamkulov, A. Makishev, M. Tuleutayev, D. Sarbassov

引用次数: 1

Assessment of drought tolerance of spring wheat 春小麦抗旱性评价

Eurasian Journal of Applied Biotechnology Pub Date : 2022-11-13 DOI: 10.11134/btp.4.2022.12

L. Zotova, A. Zhumalin, T. Savin, S. A. Dzhataev, A. Nuralov, Gulmira Khassanova

引用次数: 0

ISOLATION AND SCREENING OF ACTIVE CULTURES OF LACTIC ACID BACTERIA FOR INCLUSION IN THE COMPOSITION OF THE STARTER IN THE PREPARATION OF CHEESE FROM WHEY 乳清制奶酪发酵剂中乳酸菌活性培养物的分离和筛选

Eurasian Journal of Applied Biotechnology Pub Date : 2022-10-28 DOI: 10.11134/btp.4.2022.11

I. Tynybayeva, G. Zhakupova, T. Tultabayeva, A. Nurtayeva, A. T. Sagandyk, S.K. Bekbay, Z. Sarmurzina

{"title":"ISOLATION AND SCREENING OF ACTIVE CULTURES OF LACTIC ACID BACTERIA FOR INCLUSION IN THE COMPOSITION OF THE STARTER IN THE PREPARATION OF CHEESE FROM WHEY","authors":"I. Tynybayeva, G. Zhakupova, T. Tultabayeva, A. Nurtayeva, A. T. Sagandyk, S.K. Bekbay, Z. Sarmurzina","doi":"10.11134/btp.4.2022.11","DOIUrl":"https://doi.org/10.11134/btp.4.2022.11","url":null,"abstract":"The starter cultures used to develop fermented milk products play a critical role in ensuring the quality of the final product in terms of organoleptic properties, nutritional value, and storage. The quality of the product depends on the strain and is due to differences between strains in the presence and activity of metabolic pathways. The selection of competitive bacterial cultures and the development of bacterial starters are fundamental to establishing and maintaining industrial microorganism collections. The process of creating bacterial starters takes a lot of time and includes the main stages of work: the isolation of microbes from various sources, their development, the selection of promising strains with industrially active properties, and the subsequent isolation of pure cultures as part of a consortium of bacterial starters. Potential probiotics were isolated from samples of fermented milk products - cottage cheese, butter, etc. home-made in the Akmola region, then identified and characterized. In this study, new probiotic bacteria were selected with high antibacterial activity against pathogenic microorganisms for humans and sensitivity to antibiotics. 4 strains of the studied isolates showed resistance to antibiotics and test strains. As a result of identification by MALDI-TOF MS-biotyping, it was found that the isolates isolated from domestic fermented milk products belong to the species Leuconostoc mesenteroides, Enterococcus faecium 2 pcs, Lactobacillus paracasei.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124683807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SCREENING OF GENES RESPONSIBLE FOR β-GLUCAN SYNTHESIS IN GRAIN VARIETIES 籽粒品种β-葡聚糖合成相关基因筛选

Eurasian Journal of Applied Biotechnology Pub Date : 2022-10-28 DOI: 10.11134/btp.4.2022.10

G. Ismagulova, S. Baizhumanova, G. Iskakova, D. O. Baisaparova, A.M. Mendesh, A.A. Tussipova, Y. Skiba, E. Maltseva, D. Naizabayeva, M. Esimbekova, K. Mukin

{"title":"SCREENING OF GENES RESPONSIBLE FOR β-GLUCAN SYNTHESIS IN GRAIN VARIETIES","authors":"G. Ismagulova, S. Baizhumanova, G. Iskakova, D. O. Baisaparova, A.M. Mendesh, A.A. Tussipova, Y. Skiba, E. Maltseva, D. Naizabayeva, M. Esimbekova, K. Mukin","doi":"10.11134/btp.4.2022.10","DOIUrl":"https://doi.org/10.11134/btp.4.2022.10","url":null,"abstract":"Currently, β-glucan has a great importance for human life. Because it reduces the risks of many chronic diseases such as diabetes, cancer and digestive disorders. Due to these properties, β-glucan is a natural substance that can be used in pharmaceutical preparations for the prevention and treatment of many chronic diseases. Therefore, an analysis was made for crops rich in sources of β-glucans - 100 varieties of wheat, 50 varieties of barley and 50 varieties of oats, and a collection of genomic DNA was made. Each variety was screened to identify the genes responsible for the synthesis of β-glucans. In the frames of this study, 10 genes were considered. As a result, these genes have been identified in barley, wheat, and oat varieties.\u0000 For the isolation of β-glucan, the methods of acid extraction, microwave, and heating at high temperature were used. A modified method for isolating β-glucan from oatmeal was developed. According to the results, the acid extraction method proved to be more effective. It is easy to obtain pure β-glucan by this method, and the use of citric acid is safer and more affordable than conventional acids. The improved acid extraction method showed that the concentration of pure β-glucan per 10 g of wheat grain was 66.6%, and the total yield of β-glucan extract increased to 11.3%.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122648037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0