Eurasian Journal of Applied Biotechnology最新文献_第3页

EFFECTIVE CLONAL MICROPROPAGATION OF MALUS SIEVERSII FROM SEEDS 海参种子无性系微繁殖的研究

Eurasian Journal of Applied Biotechnology Pub Date : 2023-07-04 DOI: 10.11134/btp.2.2023.3

L. Yerbolova, S. Baizhumanova, A. Rakhatkyzy, K. Aubakirova, Zh. Bakytzhanova, S. Kazybayeva, Zh. Kadirsizova, N. Galiakparov

引用次数: 0

STUDY OF THE DIVERSITY OF THE ALGOFLORA OF THE IRTYSH RIVER AND ISOLATION OF NEW STRAINS OF PHOTOTROPHIC MICROORGANISMS 额尔齐斯河藻类植物多样性及光养微生物新菌株的分离

Eurasian Journal of Applied Biotechnology Pub Date : 2023-07-04 DOI: 10.11134/btp.2.2023.5

N. Shissenbayeva, A. Sadvakasova, N. Akmuhanova, F. Sarsekeeva, M. Bauenova, A. Kanshora

引用次数: 0

DRUG-RESISTANCE AND COMPENSATORY MUTATIONS IN MYCOBACTERIUM TUBERCULOSIS 结核分枝杆菌的耐药性和代偿性突变

Eurasian Journal of Applied Biotechnology Pub Date : 2023-07-04 DOI: 10.11134/btp.2.2023.1

D. Auganova, A. Akisheva, A. Tsepke, P. Tarlykov

引用次数: 0

ISOLATION AND IDENTIFICATION OF LACTOSE-FERMENTING YEAST CELLS FROM DAIRY PRODUCTS 乳制品中乳糖发酵酵母细胞的分离与鉴定

Eurasian Journal of Applied Biotechnology Pub Date : 2023-05-19 DOI: 10.11134/btp.1.2023.10

G.M. Myrzakhmetova, P. Ualieva, G. Abdieva

{"title":"ISOLATION AND IDENTIFICATION OF LACTOSE-FERMENTING YEAST CELLS FROM DAIRY PRODUCTS","authors":"G.M. Myrzakhmetova, P. Ualieva, G. Abdieva","doi":"10.11134/btp.1.2023.10","DOIUrl":"https://doi.org/10.11134/btp.1.2023.10","url":null,"abstract":"One of the most valuable substrates in terms of the composition of important nutritional and biological factors is dairy products. The direct use of dairy products for food purposes is characterized by a combination of high acidity and special organoleptic characteristics. Yeast cells play the role of active ingredients in them, as they have a pronounced lacto- and bifidogenic effect. Lactose-fermenting yeasts are considered one of the main objects of molecular genetics and are widely used as producers of a number of biologically active substances. In recent years, a fundamentally new direction of industrial processing of milk whey and chubat has been actively and purposefully formed, as well as the basis for obtaining derivative components, which are targeted products. The purpose of the research work: isolation and identification of lactose-fermenting yeasts from milk whey and chubat. Growth dynamics of yeast strains were studied in Sabouro's nutrient medium, under aerobic growth conditions, and were also carried out using the methods of Bradford and Glushanov. In the course of the study, the microbiological indicators and taxonomic composition of the microbial community of milk whey of \"Amiran\" LLP and chubat of \"Sarzhailau\" LLP were studied. Physico-chemical and organoleptic characteristics of dairy products were studied. 2 yeast strains were isolated from substrate samples. The morphological and cultural properties of the isolated yeasts were studied, and as a result, IL1 and IL2 strains were identified as IL1-Pichia fermentans, IL2-Pichia fermentans.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129783226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GENETIC BARCODING FOR SURVEILLANCE OF VIRULENCE PLASMIDS IN METAGENOMIC SAMPLES OF MICE GUT MICROFLORA 遗传条形码用于监测小鼠肠道菌群宏基因组样本中的毒力质粒

Eurasian Journal of Applied Biotechnology Pub Date : 2023-05-02 DOI: 10.11134/btp.1.2023.9

S.V. Shilov, I. Korotetskiy, O.N. Reva, T. Kuznetsova, N. Zubenko, L.N. Ivanova, R. Parenova, T.Kh. Izmailov

{"title":"GENETIC BARCODING FOR SURVEILLANCE OF VIRULENCE PLASMIDS IN METAGENOMIC SAMPLES OF MICE GUT MICROFLORA","authors":"S.V. Shilov, I. Korotetskiy, O.N. Reva, T. Kuznetsova, N. Zubenko, L.N. Ivanova, R. Parenova, T.Kh. Izmailov","doi":"10.11134/btp.1.2023.9","DOIUrl":"https://doi.org/10.11134/btp.1.2023.9","url":null,"abstract":"Outbreaks of nosocomial infections strike the public health system around the world. Current surveillance systems for infectious agents either take too long to identify pathogens or lack the necessary sensitivity to distinguish the virulent strains from the benign ones in the resident microflora. To address this problem, we propose a method for improving the sensitivity and specificity of detection of virulent clonal lines of bacteria or individual virulence plasmids in metagenomic samples. In this study, we used previously developed computational tools, Barcode Generator and Barcoding 2.0, to analyse gut microflora of laboratory mice infected with a pathogenic multidrug-resistant S. aureus and treated with cefazolin, an iodine-containing complex CC-195, and by a combinatorial treatment. We searched the metagenomic samples for representatives of pathogenic microflora using diagnostic genetic barcodes, which were designed based on the whole genome sequences of a collection of clinical isolates. Our results demonstrated a practical applicability of these diagnostic barcodes to monitor clonal lines of pathogens and even individual virulence plasmids in the environment. We also found that the novel drug CC-195 promotes a speedy restoration of the normal gut microflora disturbed by infection and administration of the antibiotic cefazolin.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129996254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DETERMINATION OF INDICATORS OF THE DETERIORATION OF THE EPIDEMIOLOGICAL AND EPIZOOTOLOGICAL SITUATIONS 确定流行病学和动物流行病情况恶化的指标

Eurasian Journal of Applied Biotechnology Pub Date : 2023-04-13 DOI: 10.11134/btp.1.2023.2

N. Sikhayeva, K. Zakarya, A.A. Adilova, G.S. Zhubanova, D.T. Zharmuhambetova, A.S. Abeldinova, A. Abdybekova, A. Rsaliev

{"title":"DETERMINATION OF INDICATORS OF THE DETERIORATION OF THE EPIDEMIOLOGICAL AND EPIZOOTOLOGICAL SITUATIONS","authors":"N. Sikhayeva, K. Zakarya, A.A. Adilova, G.S. Zhubanova, D.T. Zharmuhambetova, A.S. Abeldinova, A. Abdybekova, A. Rsaliev","doi":"10.11134/btp.1.2023.2","DOIUrl":"https://doi.org/10.11134/btp.1.2023.2","url":null,"abstract":"Major outbreaks of infectious diseases have demonstrated the need for new approaches to biosecurity, which include efficient planning and implementation of infection prevention and control measures. This work is not possible without the early determination of relevant indicators.\u0000The purpose of the work is the analysis of indicators in determining the deterioration of the epidemiological and epizootological situation.\u0000Results. The methodological approaches to the collection of information, the definition of indicators and their evaluation were analyzed. The analysis of the role of indicators in assessing the epidemiological and epizootological situation in terms of diseases was carried out. The main indicators of the deterioration of the epidemiological and epizootological situation for such diseases as coronavirus infection, anthrax, brucellosis, and plague have been identified.\u0000Conclusion. The following three general indicators assessing the deterioration of the epidemiological situation were identified: an increase in the incidence, a decrease in the coverage of preventive vaccinations, and the ignition of dormant foci of diseases. To assess the epizootic situation, additional indicators were identified, such as an increase in the incidence in animal population and changes in natural, climatic and soil conditions. Indicators of deterioration of the epidemiological situation may vary depending on the specific disease and its mode of transmission.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124960262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE INFLUENCE OF PHYSICAL FACTORS ON THE GROWTH OF BACTERIA-ANTAGONIST BACILLUS SUBTILIS BIM B-760 D 物理因素对枯草芽孢杆菌b760d生长的影响

Eurasian Journal of Applied Biotechnology Pub Date : 2023-04-13 DOI: 10.11134/btp.1.2023.6

V. Kuptsov, E. Y. Shmyga, E. Kolomiets, R. Nagorny, V.U. Plavsky

引用次数: 0

PRIMING OF CHIKENS WITH LIVE AND INACTIVATED IBС VACCINE 给鸡注射活疫苗和灭活疫苗ibС

Eurasian Journal of Applied Biotechnology Pub Date : 2023-04-13 DOI: 10.11134/btp.1.2023.3

V. A. Babak, A. Gusev

引用次数: 0

BIOLOGICAL FEATURES OF THE SARS-COV-2 VIRUS FROM DOMESTIC ANIMALS IN THE TERRITORY OF THE RK 来自英国境内家畜的sars-cov-2病毒的生物学特征

Eurasian Journal of Applied Biotechnology Pub Date : 2023-04-13 DOI: 10.11134/btp.1.2023.8

S. Sadikalieva, E. A. Shayakhmetov, A. Nurpeisova, K.A. Shoraуeva, Z. Abay, A.D. Omurtaу, S.K. Kopeev, A. Nakhanov, A. A. Terebay, B. Myrzakhmetova, N. Kozhabergenov, Yergaly Abduraimov

{"title":"BIOLOGICAL FEATURES OF THE SARS-COV-2 VIRUS FROM DOMESTIC ANIMALS IN THE TERRITORY OF THE RK","authors":"S. Sadikalieva, E. A. Shayakhmetov, A. Nurpeisova, K.A. Shoraуeva, Z. Abay, A.D. Omurtaу, S.K. Kopeev, A. Nakhanov, A. A. Terebay, B. Myrzakhmetova, N. Kozhabergenov, Yergaly Abduraimov","doi":"10.11134/btp.1.2023.8","DOIUrl":"https://doi.org/10.11134/btp.1.2023.8","url":null,"abstract":"There are limited data in the literature on the infection of domestic animals with SARS-CoV-2. Therefore, this article investigated biological samples (28 samples) taken from domestic animals on the territory of the Republic of Kazakhstan as part of a monitoring study.\u0000Using the ALPREP kit, total RNA was isolated by magnetic sorption, and using the ALSENSE-SARS-Cov-2 - RT-qPCR kit, the SARS-Cov-2 virus was detected from 7 samples. The SARS-Cov-2 virus was cultivated by the in vitro method on a Vero cell culture, and the biological activity of the accumulated virus-containing suspension was determined by the Reed and Muench method. A micrograph was taken on an electron microscope, which was used to determine the virus's size, shape and structure.\u0000As a result, a virus-containing suspension with the biological activity of 5.83±0.08 TCD50/cm3 was obtained on a Vero cell culture.\u0000The data obtained again indicates that domestic animals can also suffer from coronavirus and be carriers of this disease. It is becoming increasingly clear that the virus can infect and multiply in the organs of various farm and domestic animals.","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127248354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

WHOLE EXOME SEQUENCING OF A PATIENT WITH MORBID OBESITY: TRIO ANALYSIS, PRELIMINARY RESULTS 病态肥胖患者的全外显子组测序:三重奏分析，初步结果

Eurasian Journal of Applied Biotechnology Pub Date : 2023-04-13 DOI: 10.11134/btp.1.2023.5

N. Sikhayeva, A. Nakysh, T. Utupov, E. Zholdybayeva

{"title":"WHOLE EXOME SEQUENCING OF A PATIENT WITH MORBID OBESITY: TRIO ANALYSIS, PRELIMINARY RESULTS","authors":"N. Sikhayeva, A. Nakysh, T. Utupov, E. Zholdybayeva","doi":"10.11134/btp.1.2023.5","DOIUrl":"https://doi.org/10.11134/btp.1.2023.5","url":null,"abstract":"Morbid obesity is a severe form of obesity that leads to numerous cardiovascular, metabolic diseases and cancers, as well as increased mortality. Whole-exome sequencing is the effective tool for studying more extreme forms of disease, such as morbid obesity. The aim of the study is to identify candidate genes involved in the development of morbid obesity using whole exome sequencing.\u0000Here in this study, a family from Kazakh cohort having two siblings, one unaffected and one affected with morbid obesity was enrolled. Whole Exome Sequencing (WES) of trio with one affected and unaffected parents was done.\u0000The trio analysis revealed a number of potential candidate genes predisposing to morbid obesity. Three genetic models were used: recessive, de novo and compound heterozygote models. In the recessive model, one variant (rs116253946) in the KIAA1671 was identified as a candidate. In the de novo model, 15 polymorphisms located in 10 different genes were identified. In the compound heterozygote model, 6 polymorphisms, located in FRG1 and MST1L genes, were identified.\u0000This work presents the preliminary results of the study. Twelve genes identified by WES may be involved in the development of morbid obesity, and are grouped into three main pathophysiological pathways (immune response: MST1L, HLA-DRB5, PRRC2A, KIR2DS4; cell division and structure: CEP170, TEKT4; neuronal response: OR2T34, ANKMY2) or their function remains unknown to date (KIAA1671, TMEM191C, PPP6R2, FRG1).","PeriodicalId":101644,"journal":{"name":"Eurasian Journal of Applied Biotechnology","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132102794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0