JOURNAL OF CELLULAR AND MOLECULAR MEDICINE最新文献_第8页

A Machine Learning Model for Diagnosing Opportunistic Infections in HIV Patients: Broad Applicability Across Infection Types 诊断HIV患者机会性感染的机器学习模型：跨感染类型的广泛适用性

IF 5.3

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-03-23 DOI: 10.1111/jcmm.70497

Hao Chen, Fanxuan Chen, Yijun Wang, Enna Cai, Wangzheng Pan, Yichen Li, Zefei Mo, Hao Lou, Chufan Ren, Chenyue Dai, Xingbo Shan, Hui Ye, Zhenwei Xu, Pu Dong, Han Zhou, Shuya Xu, Tianye Zhu, Mingzhi Su, Xingguo Miao, Xiaoqu Hu, Liang Hong, Yi Wang, Feifei Su

{"title":"A Machine Learning Model for Diagnosing Opportunistic Infections in HIV Patients: Broad Applicability Across Infection Types","authors":"Hao Chen, Fanxuan Chen, Yijun Wang, Enna Cai, Wangzheng Pan, Yichen Li, Zefei Mo, Hao Lou, Chufan Ren, Chenyue Dai, Xingbo Shan, Hui Ye, Zhenwei Xu, Pu Dong, Han Zhou, Shuya Xu, Tianye Zhu, Mingzhi Su, Xingguo Miao, Xiaoqu Hu, Liang Hong, Yi Wang, Feifei Su","doi":"10.1111/jcmm.70497","DOIUrl":"https://doi.org/10.1111/jcmm.70497","url":null,"abstract":"Opportunistic infections (OIs) are the leading cause of hospitalisation and mortality among Human Immunodeficiency Virus-infected (HIV-infected) patients. The diverse pathogen types and intricate clinical manifestations associated present a formidable challenge to the timely diagnosis of these infections. This study aims to use machine learning techniques to develop a diagnostic model that quickly identifies whether HIV-infected patients have any type of OIs, without being limited to specific infections, thus adapting to various clinical scenarios. This study is a retrospective cohort study that collected clinical data from HIV-infected patients at four healthcare organisations in China. A total of twelve machine learning classification algorithms were employed for the purposes of model training and evaluation. Additionally, feature reduction was conducted through the implementation of an importance ranking, with the objective of eliminating any redundant features. In conclusion, both the five features based on Shapley additive explanations (procalcitonin, haemoglobin, lymphocyte, creatinine, platelet) and the five features based on Permutation Importance explanations (procalcitonin, lymphocyte, haemoglobin, creatinine, indirect bilirubin) achieved the highest F1 score when evaluated using the adaptive boosting classifier model. The scores on the test set were 0.9016 and 0.9063, respectively, which significantly outperformed the best 32-feature model, gradient boosting classifier, which had a test set F1 score of 0.8991.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70497","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine Learning-Based Glycolipid Metabolism Gene Signature Predicts Prognosis and Immune Landscape in Oesophageal Squamous Cell Carcinoma 基于机器学习的糖脂代谢基因特征可预测食管鳞状细胞癌的预后和免疫格局

IF 5.3

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-03-22 DOI: 10.1111/jcmm.70434

Lin Zhu, Feng Liang, Xue Han, Bin Ye, Lei Xue

{"title":"Machine Learning-Based Glycolipid Metabolism Gene Signature Predicts Prognosis and Immune Landscape in Oesophageal Squamous Cell Carcinoma","authors":"Lin Zhu, Feng Liang, Xue Han, Bin Ye, Lei Xue","doi":"10.1111/jcmm.70434","DOIUrl":"10.1111/jcmm.70434","url":null,"abstract":"Using machine learning approaches, we developed and validated a novel prognostic model for oesophageal squamous cell carcinoma (ESCC) based on glycolipid metabolism-related genes. Through integrated analysis of TCGA and GEO datasets, we established a robust 15-gene signature that effectively stratified patients into distinct risk groups. This signature demonstrated superior prognostic value and revealed significant associations with immune infiltration patterns. High-risk patients exhibited reduced immune cell infiltration, particularly in B cells and NK cells, alongside increased tumour purity. Single-cell RNA sequencing analysis uncovered unique cellular composition patterns and enhanced interaction intensities in the high-risk group, especially within epithelial and smooth muscle cells. Functional validation confirmed MECP2 as a promising therapeutic target, with its knockdown significantly inhibiting tumour progression both in vitro and in vivo. Drug sensitivity analysis identified specific therapeutic agents showing potential efficacy for high-risk patients. Our study provides both a practical prognostic tool and novel insights into the relationship between glycolipid metabolism and tumour immunity in ESCC, offering potential strategies for personalised treatment.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70434","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemerin's Role in Endometrial Dysfunction: Insights From Transcriptomic Analysis Chemerin在子宫内膜功能障碍中的作用：来自转录组学分析的见解。

IF 5.3

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-03-21 DOI: 10.1111/jcmm.70417

Ming Yu, Yichun Wang, Jinxuan Cai, Xinyue Dong, Hao Wang, Zichen Sun, Tianxia Xiao, Jie Chen, Mengxia Li, Chunhua Shan, Yang Dong, Jian V. Zhang

{"title":"Chemerin's Role in Endometrial Dysfunction: Insights From Transcriptomic Analysis","authors":"Ming Yu, Yichun Wang, Jinxuan Cai, Xinyue Dong, Hao Wang, Zichen Sun, Tianxia Xiao, Jie Chen, Mengxia Li, Chunhua Shan, Yang Dong, Jian V. Zhang","doi":"10.1111/jcmm.70417","DOIUrl":"10.1111/jcmm.70417","url":null,"abstract":"Endometrium, the lining of the uterus, changes dynamically in response to fluctuations in ovarian hormones. The proper endocrine environment regulates endometrial functions: menstruation and supporting pregnancy. Obesity is closely related to endometrial dysfunction, which seriously affects women's health and fertility, but the pathological mechanism is unknown. Chemerin is an adipokine involved in multiple biological events such as immunity and metabolism by acting on its functional receptors. This study aimed to characterise the effects of chemerin on human endometrial epithelial cells by RNA-Seq. 12Z cells were utilised as the model because immunoblot results showed that they expressed endometrial markers, epithelial markers and functional receptors for chemerin. Principal component analysis (PCA) showed that chemerin treatment significantly altered the transcriptome. Differential Expression Analysis found 388 significant differentially expressed genes (DEG) in the chemerin treatment group compared with the controls. Gene Set Enrichment Analysis (GSEA) showed that chemerin inhibited lipid metabolism and induced the epithelial-mesenchymal transition (EMT)-like process and cellular senescence. More importantly, GSEA and immunoblots showed that chemerin restrained the STAT3 signalling pathway, which is required for endometrial receptivity establishment. Collectively, these findings provide new evidence that over-produced chemerin underlying the endometrial dysfunctions in obesity.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TIMP-2 Modulates 5-Fu Resistance in Colorectal Cancer Through Regulating JAK–STAT Signalling Pathway TIMP-2通过调控JAK-STAT信号通路调控结直肠癌5-Fu耐药性

IF 5.3

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-03-21 DOI: 10.1111/jcmm.70470

Chuchu Xu, Renjun Zhu, Qingfeng Dai, Yaoqing Li, Gengyuan Hu, Kelong Tao, Yuhong Xu, Guangen Xu, Guolin Zhang

{"title":"TIMP-2 Modulates 5-Fu Resistance in Colorectal Cancer Through Regulating JAK–STAT Signalling Pathway","authors":"Chuchu Xu, Renjun Zhu, Qingfeng Dai, Yaoqing Li, Gengyuan Hu, Kelong Tao, Yuhong Xu, Guangen Xu, Guolin Zhang","doi":"10.1111/jcmm.70470","DOIUrl":"10.1111/jcmm.70470","url":null,"abstract":"The main reason for the failure of chemotherapy therapies based on 5-Fluorouracil (5-Fu) is the development of resistance to 5-Fu in cancer patients, particularly those with colorectal cancer. Tissue inhibitor of metalloproteinases 2 (TIMP-2) has been shown to be associated with colorectal cancer (CRC), but its correlation with 5-Fu resistance in colorectal cancer has not been thoroughly studied. We screen the expression of different cytokines through Cytokine array. CCK-8 assay was conducted to evaluate the IC50 of 5-Fu and cell proliferation. ELISA and RT-qPCR were performed to detect TIMP-2 expression levels in cells and patient serum. Western blotting was utilised to analyse the differences in the expression of proteins related to signalling pathways in cells. Through cytokine array screening, we found that the expression of TIMP-2 was significantly increased in CRC drug-resistant cell lines. In addition, the expression of TIMP-2 in the serum of patients with CRC resistance to 5-Fu was significantly increased. Subsequent mechanistic experiments showed that TIMP-2 regulated the resistance of CRC cells to 5-Futhrough the JAK–STAT signalling pathway. Moreover, anti-TIMP-2 antibody or small molecule drug LY2784544 targeting the JAK–STAT signalling pathway can effectively reverse the resistance of CRC cells to 5-Fu. It is exactly TIMP-2 that mediates the resistance of CRC to 5-Fu through the JAK–STAT signalling pathway. Targeting drugs for TIMP-2 or the JAK–STAT signalling pathway are expected to be opportunities to reverse 5-Fu resistance in CRC.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70470","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomics Analysis of Functional Activity Changes in Residual Tumour Cells After IOCS Treatment IOCS治疗后残余肿瘤细胞功能活性变化的代谢组学分析。

IF 5.3

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-03-20 DOI: 10.1111/jcmm.70452

Lai-wei You, Jinhuo Wang, Dan Yin, Bao-ji Hu, Yong Cheng, Xue-fei Wang, Hao Li, Jianrong Guo

{"title":"Metabolomics Analysis of Functional Activity Changes in Residual Tumour Cells After IOCS Treatment","authors":"Lai-wei You, Jinhuo Wang, Dan Yin, Bao-ji Hu, Yong Cheng, Xue-fei Wang, Hao Li, Jianrong Guo","doi":"10.1111/jcmm.70452","DOIUrl":"10.1111/jcmm.70452","url":null,"abstract":"Hepatocellular carcinoma (HCC) is a serious and often lethal cancer, particularly in patients with chronic liver disease. Currently, no specific treatment has been utilised to prevent HCC. The detailed mechanism of HCC is still elusive, and this study aims to identify and characterise the functional activity changes in residual tumour cells following intraoperative cell salvage (IOCS) treatment during HCC surgery. This research is a retrospective case–control study, involving the selection of 60 patients with HCC who underwent radical surgery; then blood and tumour tissue were collected for further testing. GC–MS assay, immunofluorescence, Western blot and qRT-PCR techniques were employed. Our study found comparable demographic and baseline clinical characteristics between the experimental group (n = 30), which received IOCS treatment during surgery, and the control group (n = 30), which did not receive IOCS treatment, validating subsequent analyses. Metabolomic analysis revealed six key metabolites differing between groups, indicating improvement in liver tumours in the experimental group. TP53 expression was significantly upregulated, potentially mediating therapeutic effects. The intervention reduced HCC cell migration and apoptosis, decreased E2F1 and MDM2 protein and mRNA levels, and increased TP53 and CTNNB1 levels. These findings support the potential clinical application of the intervention in improving treatment outcomes for HCC patients, warranting further investigation to elucidate the underlying mechanisms and optimise therapeutic strategies.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Srxn1 Overexpression Protect Against Cardiac Remodelling by Inhibiting Oxidative Stress and Inflammation Srxn1过表达通过抑制氧化应激和炎症保护心脏重构。

IF 5.3

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-03-20 DOI: 10.1111/jcmm.70432

Huibo Wang, Ying Yang, Yong Ye, Xing Wei, Shen Chen, Bin Cheng, Yunbo Lv

{"title":"Srxn1 Overexpression Protect Against Cardiac Remodelling by Inhibiting Oxidative Stress and Inflammation","authors":"Huibo Wang, Ying Yang, Yong Ye, Xing Wei, Shen Chen, Bin Cheng, Yunbo Lv","doi":"10.1111/jcmm.70432","DOIUrl":"10.1111/jcmm.70432","url":null,"abstract":"Oxidative stress and inflammation are common medical issues contributing to the onset and progression of heart failure (HF). Sulfiredoxin 1 (Srxn1) is a key regulatory factor in the antioxidant response. This study aimed to examine the effect of Srxn1 in HF. We utilised transcriptome sequencing to screen for differentially expressed genes in cardiac remodelling. We overexpressed Srxn1 in the hearts using an adeno-associated virus 9 (AAV9) system through tail vein injection. C57BL/6 mice were subjected to transverse aortic constriction (TAC) for 4 weeks. Echocardiography was used to evaluate cardiac function, and cardiac remodelling was estimated by histopathology and molecular techniques. In addition, H9C2 cells were stimulated by Ang II to establish an in vitro model of cardiomyocyte hypertrophy, and the effects of Srxn1 overexpression on the inflammatory pathways and oxidative stress in Ang II-stimulated H9C2 cells were examined. We found that Srxn1 is downregulated after cardiac remodelling by transcriptome sequencing. Our results revealed down-regulated levels of Srxn1 in murine hearts subjected to TAC treatment, and H9C2 challenged with Ang II. Moreover, compared with WT mice, AAV-9-Srxn1 mice exhibited dramatically ameliorated TAC-induced cardiac dysfunction, hypertrophy, fibrosis, oxidative stress, and inflammation. In terms of mechanism, both in vitro and in vivo experiments confirmed that the potential positive impacts may be linked to the inhibition of TLR4/NF-κB signalling. In summary, this study is the first to demonstrate the protective effects of Srxn1 against TAC-induced cardiac oxidative stress and inflammation, which are induced by the inhibited activation of the TLR4/NF-κB signalling pathway.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Midnolin Correlates With Anti-Tumour Immunity and Promotes Liver Cancer Progression Through β-Catenin 米诺林与抗肿瘤免疫相关，并通过β-连环蛋白促进肝癌进展。

IF 5.3

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-03-20 DOI: 10.1111/jcmm.70472

Shaobo Huang, Jinling Zhang, Ting He, Jianping Zhou, Zhigang Liu

{"title":"Midnolin Correlates With Anti-Tumour Immunity and Promotes Liver Cancer Progression Through β-Catenin","authors":"Shaobo Huang, Jinling Zhang, Ting He, Jianping Zhou, Zhigang Liu","doi":"10.1111/jcmm.70472","DOIUrl":"10.1111/jcmm.70472","url":null,"abstract":"Midnolin (MIDN) is a protein coding gene that promotes the destruction of transcription factors encoded by immediate-early genes. Previous research has found that those immediate-early genes are involved in tumour progression. However, the role of MIDN is still not clearly identified in human cancers. With the help of the TCGA, GTEx, and HPA databases, we revealed that the expression of MIDN was disordered in cancers. MIDN is a potential prognostic biomarker in liver cancer and bladder cancer. Prognostic analysis indicates that the expression level of MIDN gains survival benefits or promotes progression in multiple tumours. After analysing the sequencing results of TCGA via Gene Set Enrichment Analysis (GSEA), results suggested the regulative role of MIDN in cell proliferation and tumour immunity. Single cell sequencing results revealed that MIDN is highly expressed in several tumour tissues and also expressed in immune cells. With the help of the ESTIMATE, TIMER, and CIBERSORT databases, we analysed the immune score, immune cell infiltration, and anti-cancer immunity cycle depending on the expression of MIDN. Results showed that low MIDN levels are tightly associated with high CD4 + T and NK cell infiltration. Furthermore, mutations of MIDN in cancers were significantly associated with immune cell infiltration. This study presents a robust link between the expression of MIDN and tumour progression across multiple cancer types. The MIDN/CTNNB1/MMP9 axis promotes liver cancer progression via inducing a suppressive tumour immune microenvironment.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DDR1 Targeting HOXA6 Facilitates Bladder Cancer Progression via Inhibiting Ferroptosis 靶向HOXA6的DDR1通过抑制铁下垂促进膀胱癌进展

IF 5.3

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-03-19 DOI: 10.1111/jcmm.70410

Xin Xie, Hongchao He, Ning Zhang, Xiaojing Wang, Wenbin Rui, Danfeng Xu, Yu Zhu, Ming Tian, Wei He

{"title":"DDR1 Targeting HOXA6 Facilitates Bladder Cancer Progression via Inhibiting Ferroptosis","authors":"Xin Xie, Hongchao He, Ning Zhang, Xiaojing Wang, Wenbin Rui, Danfeng Xu, Yu Zhu, Ming Tian, Wei He","doi":"10.1111/jcmm.70410","DOIUrl":"https://doi.org/10.1111/jcmm.70410","url":null,"abstract":"Ferroptosis is an important factor affecting the progression of bladder cancer (BC). Previous studies have confirmed that discoidin domain receptor 1 (DDR1) promotes BC progression. However, the regulatory mechanisms of BC ferroptosis are largely unknown. Therefore, this study aimed to investigate the regulatory effects of DDR1 on BC cell ferroptosis. Ferroptosis-sensitive and -resistant BC cells were screened, and reverse-transcription quantitative PCR and western blotting were used to determine the expression of DDR1 in BC cells. In vitro and in vivo assays were performed to analyse the mechanisms of DDR1 in BC ferroptosis. The ferroptosis inducer erastin inhibited DDR1 expression in TCCSUP cells. The ferroptosis inhibitor ferrostatin-1 inhibited BC cell death caused by DDR1 knockdown. DDR1 increased glutathione, glutathione peroxidase 4 and solute carrier family 7 member 11 expression, while decreasing malondialdehyde and Fe2+ levels and acyl-CoA synthetase long-chain family member 4 levels and inhibiting epithelial mesenchymal transition and neurofibromin 2-yes-associated protein. These effects were abrogated by the knockdown of homeobox A6 (HOXA6). DDR1 targeting of HOXA6 facilitated BC growth and inhibited BC ferroptosis in vivo. DDR1 promotes BC progression by inhibiting ferroptosis and targeting HOXA6. Thus, DDR1 may serve as a potential therapeutic target for BC.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70410","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disease-Associated Risk Variants and Expression Levels of the lncRNA, CDKN2B-AS1, Are Associated With the Progression of HCC 疾病相关的危险变异和lncRNA CDKN2B-AS1的表达水平与HCC的进展有关

IF 5.3

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-03-19 DOI: 10.1111/jcmm.70496

Kuan-Chun Hsueh, Hsiang-Lin Lee, Kuo-Hao Ho, Lun-Ching Chang, Shun-Fa Yang, Ming-Hsien Chien

{"title":"Disease-Associated Risk Variants and Expression Levels of the lncRNA, CDKN2B-AS1, Are Associated With the Progression of HCC","authors":"Kuan-Chun Hsueh, Hsiang-Lin Lee, Kuo-Hao Ho, Lun-Ching Chang, Shun-Fa Yang, Ming-Hsien Chien","doi":"10.1111/jcmm.70496","DOIUrl":"https://doi.org/10.1111/jcmm.70496","url":null,"abstract":"The most susceptible loci of hepatocellular carcinoma (HCC) identified by genome-wide association studies are located in non-coding regions. The antisense non-coding RNA at the INK4 locus (ANRIL), also known as cyclin-dependent kinase inhibitor 2B antisense RNA 1 (CDKN2B-AS1), is a long non-coding (lnc)RNA situated within and antisense to genes encoding CDKN2A/B on chromosome 9p21.3. Single-nucleotide polymorphisms (SNPs) within CDKN2B-AS1 are associated with several cancer types, but their impacts on HCC remain unclear. In this study, we investigated the effects of CDKN2B-AS1 SNPs on both the susceptibility to HCC and its clinicopathological development. Five CDKN2B-AS1 SNP loci—rs564398 (T/C), rs1333048 (A/C), rs1537373 (G/T), rs2151280 (A/G) and rs8181047 (G/A)—were analysed using a TaqMan allelic discrimination assay for genotyping in a cohort of 810 HCC patients and 1190 healthy controls. Under the dominant model, HCC patients with at least one minor C-allele of rs564398 showed a lower risk of liver cirrhosis (odds ratio (OR) = 0.677). Additionally, HCC patients with the GT + TT genotype of rs1537373 had a reduced risk of developing large tumours (T3 + T4) and advanced clinical stages (III/IV), particularly in the male population (OR = 0.644 and 0.679). Furthermore, data from The Cancer Genome Atlas revealed that CDKN2B-AS1 expression levels were elevated in HCC tissues compared to normal tissues and were correlated with advanced T stages, high histological grades and poor prognoses. Our findings suggest that CDKN2B-AS1 levels and its polymorphic variants at rs564398 and rs1537373 may influence the clinicopathological development and progression of HCC in a Taiwanese population.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70496","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of Diagnostic Techniques for Helicobacter pylori Infection: Insights for Effective Therapy 幽门螺杆菌感染诊断技术的比较分析：对有效治疗的见解

IF 5.3

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-03-19 DOI: 10.1111/jcmm.70487

Ahmed Mujtaba, Muhammad Suhail Ibrahim, Sana Parveen, Noreen Sarwar, Suliman A. Alsagaby, Muhammad Ahsan Raza, Mohamed A. Abdelgawad, Mohammed M. Ghoneim, Ahmed H. El-Ghorab, Samy Selim, Waleed Al Abdulmonem, Muzzamal Hussain, Tadesse Fenta Yehuala

{"title":"Comparative Analysis of Diagnostic Techniques for Helicobacter pylori Infection: Insights for Effective Therapy","authors":"Ahmed Mujtaba, Muhammad Suhail Ibrahim, Sana Parveen, Noreen Sarwar, Suliman A. Alsagaby, Muhammad Ahsan Raza, Mohamed A. Abdelgawad, Mohammed M. Ghoneim, Ahmed H. El-Ghorab, Samy Selim, Waleed Al Abdulmonem, Muzzamal Hussain, Tadesse Fenta Yehuala","doi":"10.1111/jcmm.70487","DOIUrl":"https://doi.org/10.1111/jcmm.70487","url":null,"abstract":"Effective therapy against Helicobacter pylori hinges on a timely and accurate diagnosis. The objective is to assess H. pylori infection in dyspeptic patients and compare various indicative tests. After approval, gastrointestinal biopsies and blood samples of 96 subjects exhibiting gastroduodenal symptoms were collected; both invasive and non-invasive tests were employed to analyse the samples. Results revealed 40 cases (41.67%) positive for H. pylori via histopathology and rapid urease testing, while 46 subjects tested positive for IgA and IgG antibodies via ELISA. Eighteen biopsies showed positivity in the culture test, corroborated by endoscopic examination and biochemical assessments (urease, catalase and oxidase). The isolates showed various degrees of resistance to antibiotics, while polymyxin B showed the highest (100%) followed by amoxicillin (88.90%) and kanamycin (77.78%). Additionally, the CagA gene presence was detected in 18 individuals through molecular methods. Sensitivity and specificity percentages (%) varied among diagnostic methods: histopathology (95/77), rapid urease (100/83.5), gram staining (85.7/90), IgG serology (100/66.6), IgA serology (100/79.5), PCR (100/75), RUT and IgG serology combination (100/79.04), and RUT, Gram staining and IgG serology combination (100/92.4), respectively. PCR emerged as the most reliable test. In the current investigation, other tests also exhibited high sensitivity and specificity values. Thus, employing comparative detection methods rather than relying solely on one methodology is advisable for accurate detection.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70487","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0