JOURNAL OF CELLULAR AND MOLECULAR MEDICINE最新文献

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Potential pro-tumour cytokine in oral squamous cellular carcinoma: IL37 口腔鳞状细胞癌中潜在的促肿瘤细胞因子:IL37。
IF 5.3
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2024-11-05 DOI: 10.1111/jcmm.70167
Ying Yan, Jun Li, Yungang He, Ping Ji, Jie Xu, Yong Li
{"title":"Potential pro-tumour cytokine in oral squamous cellular carcinoma: IL37","authors":"Ying Yan,&nbsp;Jun Li,&nbsp;Yungang He,&nbsp;Ping Ji,&nbsp;Jie Xu,&nbsp;Yong Li","doi":"10.1111/jcmm.70167","DOIUrl":"10.1111/jcmm.70167","url":null,"abstract":"<p>Inflammation and immunosuppression are important features of tumours, including oral squamous cellular carcinoma (OSCC). Interleukin 37 (IL37), a cytokine known for the ability to suppress inflammation and immunity, shows two seemingly contradictory functions in tumours. This study aims to investigate the mechanism that regulates IL37 and its role in OSCC progression. Herein, IL37, CD86 and CD206 in OSCC specimens were determined. Hypoxia, MCC950 and siRNA-Gasdermin D (GSDMD) were utilised to investigate the mechanism of IL37 production and release. Animal experiments were established to examine the role of IL37 in OSCC growth in vivo. We found the levels of IL37 are elevated in OSCC tissues compared with normal oral mucosa. In cell experiments, hypoxia was proved to be a vital facilitator in IL37 expression and release. Mechanically, hypoxia promoted IL37 expression through the activation of NACHT–LRR–PYD-containing protein 3 (NLRP3) inflammasome, and promoted IL37 release via GSDMD. Furthermore, IL37 levels in OSCC specimens are positively correlated with the number of M2 macrophages, but negatively with M1. Further studies revealed IL37 facilitated OSCC progression via promoting macrophage polarization from M1 to M2 and enhancing tumour cell proliferation. Thus, IL37 could be a promising target for OSCC treatment in the future.</p>","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70167","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Downregulation of GLYAT correlates with tumour progression and poor prognosis in hepatocellular carcinoma GLYAT 的下调与肝细胞癌的肿瘤进展和预后不良有关。
IF 5.3
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2024-11-04 DOI: 10.1111/jcmm.70197
Fengchen Jiang, Shuiping Zhou, Chuanlong Xia, Jiale Lu, Bin Wang, Xiaowei Wang, Jiandong Shen, Wei Ding, Mengjie Yin, Feng Dai, Shouzhong Fu
{"title":"Downregulation of GLYAT correlates with tumour progression and poor prognosis in hepatocellular carcinoma","authors":"Fengchen Jiang,&nbsp;Shuiping Zhou,&nbsp;Chuanlong Xia,&nbsp;Jiale Lu,&nbsp;Bin Wang,&nbsp;Xiaowei Wang,&nbsp;Jiandong Shen,&nbsp;Wei Ding,&nbsp;Mengjie Yin,&nbsp;Feng Dai,&nbsp;Shouzhong Fu","doi":"10.1111/jcmm.70197","DOIUrl":"10.1111/jcmm.70197","url":null,"abstract":"<p>Glycine N-acyltransferase (GLYAT), known to influence glycine metabolism, has been implicated in the progression of various malignant tumours. However, its clinical relevance in hepatocellular carcinoma (HCC) remains unexplored. Here, GLYAT expression levels in HCC tissues were significantly reduced compared to normal liver tissues. Similarly, GLYAT expression levels in Huh 7, HepG2, PLC and SK-HEP1 were lower than those in LO2. Receiver operating characteristic curve analysis demonstrated that GLYAT exhibited good diagnostic performance for HCC. Kaplan–Meier analyses suggested that decreased GLYAT expression was correlated with poorer progress in HCC. Low GLYAT expression was significantly associated with gender and histologic grade. Multivariate Cox regression analysis identified low GLYAT expression and T stage as independent prognostic factors. Nomograms based on GLYAT mRNA expression and T stage showed good concordance with actual survival rates at 1, 2, 3 and 5 years. Moreover, GLYAT downregulation in the Huh 7 cell line enhanced cell proliferation, invasion and migration abilities, while GLYAT overexpression in the HepG2 cell line inhibited these abilities. HCC patients with low GLYAT expression exhibited a predisposition to immune escape and poor response to immunotherapy. This research revealed that GLYAT holds promise as both a prognostic biomarker and a potential therapeutic target in HCC.</p>","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70197","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The combination of FLCWK with 5-FU inhibits colon cancer and multidrug resistance by activating PXR to suppress the IL-6/STAT3 pathway FLCWK与5-FU联用可通过激活PXR来抑制IL-6/STAT3通路,从而抑制结肠癌和多药耐药性。
IF 5.3
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2024-11-04 DOI: 10.1111/jcmm.70185
Lifan Zhong, Qianru Wang, Zhixiong Kou, Lianfang Gan, Zhaoxin Yang, Junhua Pan, Ling Huang, Yunqiang Chen
{"title":"The combination of FLCWK with 5-FU inhibits colon cancer and multidrug resistance by activating PXR to suppress the IL-6/STAT3 pathway","authors":"Lifan Zhong,&nbsp;Qianru Wang,&nbsp;Zhixiong Kou,&nbsp;Lianfang Gan,&nbsp;Zhaoxin Yang,&nbsp;Junhua Pan,&nbsp;Ling Huang,&nbsp;Yunqiang Chen","doi":"10.1111/jcmm.70185","DOIUrl":"10.1111/jcmm.70185","url":null,"abstract":"<p>5-fluorouracil (5-FU) is a preferred chemotherapeutic agent for the treatment of colon cancer. Nonetheless, its clinical effectiveness is frequently hampered by suboptimal therapeutic outcomes and the emergence of drug resistance. Therefore, there exists a pressing demand for novel therapeutic agents to circumvent chemoresistance. The pregnane X receptor (PXR) exerts a pivotal regulatory influence on the proliferation, invasion, and chemoresistance mechanisms in colon cancer. Activation of PXR drives up the transcription of the multidrug resistance gene (MDR1), thus prompting the expression of P-glycoprotein (P-gp) responsible for conferring tumour resistance. This study scrutinized the potential of Fengliao Changweikang (FLCWK) in augmenting the efficacy of 5-FU in the management of colon cancer. To this end, we engineered colon cancer cells with varied levels of PXR expression via lentiviral transfection, subsequently validating the findings in nude mice. By means of MTT assays, flow cytometry apoptosis analysis, Western blotting and immunofluorescence, we probed into the prospective impacts of FLCWK and 5-FU on cellular viability and resistance. Our results revealed that while upregulation of PXR amplified the therapeutic benefits in colon cancer treatment, it concurrently heightened resistance levels. FLCWK demonstrated a capacity to reduce P-gp expression, with the combined administration of FLCWK and 5-FU effectively reversing resistance mechanisms. Furthermore, activation of PXR was found to impede the IL-6/STAT3 signalling pathway. In an effort to mimic the development of colon cancer, we established an azomethane oxide (AOM)/ dextran sodium sulfate (DSS) mouse model, showing that FLCWK bolstered the inhibitory effects of 5-FU, impeding the progression of colon cancer. In summation, our findings point towards the potential of FLCWK in the treatment of colon cancer, particularly in strengthening the therapeutic efficacy of 5-FU in the prevention and control of the disease.</p>","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70185","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exosomal miR-155-5p promote the occurrence of carotid atherosclerosis 外泌体 miR-155-5p 促进颈动脉粥样硬化的发生
IF 5.3
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2024-11-04 DOI: 10.1111/jcmm.70187
Wen-Wen Yang, Qing-Xiang Li, Fei Wang, Xin-Ran Zhang, Xian-Li Zhang, Meng Wang, Dong Xue, Ying Zhao, Lu Tang
{"title":"Exosomal miR-155-5p promote the occurrence of carotid atherosclerosis","authors":"Wen-Wen Yang,&nbsp;Qing-Xiang Li,&nbsp;Fei Wang,&nbsp;Xin-Ran Zhang,&nbsp;Xian-Li Zhang,&nbsp;Meng Wang,&nbsp;Dong Xue,&nbsp;Ying Zhao,&nbsp;Lu Tang","doi":"10.1111/jcmm.70187","DOIUrl":"10.1111/jcmm.70187","url":null,"abstract":"<p>Periodontitis is a significant independent risk factor for atherosclerosis. Yet, the exact mechanism of action is still not fully understood. In this study, we investigated the effect of exosomes-miR-155-5p derived from periodontal endothelial cells on atherosclerosis in vitro and in vivo. Higher expression of miR-155-5p was detected in the plasma exosomes of patients with chronic periodontitis (CP) and carotid atherosclerosis (CAS) compared to patients with CP. Also, the expression level of miR-155-5p was associated with the severity of CP. miR-155-5p-enriched exosomes from HUVECs increased the angiogenesis and permeability of HAECs and promoted the expression of angiogenesis, permeability, and inflammation genes. Along with the overexpression or inhibition of miR-155-5p, the biological effect of HUVECs-derived exosomes on HAECs changed correspondingly. In ApoE−/− mouse models, miR-155-5p-enriched exosomes promoted the occurrence of carotid atherosclerosis by increasing permeable and angiogenic activity. Collectively, these findings highlight a molecular mechanism of periodontitis in CAS, uncovering exosomal miR-155-5p derived periodontitis affecting carotid endothelial cells in an ‘exosomecrine’ manner. Exosomal miR-155-5p may be used as a biomarker and target for clinical intervention to control this intractable disease in future, and the graphic abstract was shown in Figure S1.</p>","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70187","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CARD9 protein SUMOylation regulates HOXB5 nuclear translocation and Parkin-mediated mitophagy in myocardial I/R injury CARD9 蛋白 SUMOylation 在心肌 I/R 损伤中调控 HOXB5 核转位和 Parkin 介导的有丝分裂。
IF 5.3
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2024-11-04 DOI: 10.1111/jcmm.70195
Yuanbin Li, Yuting Tang, Xu Yan, Hui Lin, Wanjin Jiang, Luwei Zhang, Hu Zhao, Zhuang Chen
{"title":"CARD9 protein SUMOylation regulates HOXB5 nuclear translocation and Parkin-mediated mitophagy in myocardial I/R injury","authors":"Yuanbin Li,&nbsp;Yuting Tang,&nbsp;Xu Yan,&nbsp;Hui Lin,&nbsp;Wanjin Jiang,&nbsp;Luwei Zhang,&nbsp;Hu Zhao,&nbsp;Zhuang Chen","doi":"10.1111/jcmm.70195","DOIUrl":"10.1111/jcmm.70195","url":null,"abstract":"<p>Myocardial injury induced by ischemia–reperfusion (I/R) remains a difficult clinical problem. However, the exact mechanisms underlying I/R-induced have yet to be clarified. CARD9 is an important cytoplasmic-binding protein. In this study, an immunocoprecipitation assay showed that SUMOylation of the CARD9 protein promoted the binding of CARD9 to HOXB5, but hindered the O-GlcNAc glycosylation of HOXB5, a predicted transcription factor of Parkin and a key factor in mitophagy. O-GlcNAc glycosylation is an important signal for translocation of proteins from the cytoplasm to the nucleus. CARD9 protein SUMOylation is regulated by PIAS3, which is related to I/R-induced myocardial injury. Therefore, we propose that knockdown of PIAS3 inhibits SUMOylation of the CARD9 protein, facilitates the dissociation of CARD9 and HOXB5, which increases the O-GlcNAc-mediated glycosylation of HOXB5, while the resulting HOXB5 nuclear translocation promotes Parkin-induced mitophagy and alleviates myocardial I/R injury.</p>","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70195","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibiting YAP1 reduced abdominal aortic aneurysm formation by suppressing adventitial fibroblast phenotype transformation and migration 抑制 YAP1 可抑制临近纤维母细胞的表型转化和迁移,从而减少腹主动脉瘤的形成。
IF 5.3
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2024-11-04 DOI: 10.1111/jcmm.70159
Cuiping Xie, Yanting Hu, Zhehui Yin
{"title":"Inhibiting YAP1 reduced abdominal aortic aneurysm formation by suppressing adventitial fibroblast phenotype transformation and migration","authors":"Cuiping Xie,&nbsp;Yanting Hu,&nbsp;Zhehui Yin","doi":"10.1111/jcmm.70159","DOIUrl":"10.1111/jcmm.70159","url":null,"abstract":"<p>The adventitial fibroblast (AF) is the most abundant cell in the vascular adventitia, a few studies had confirmed that AF contributed to abdominal aortic aneurysm (AAA) development; YAP1 involved in several vascular diseases by promoting AF transformed to myofibroblast, the role of YAP1 in AAA is not clear yet. This study aims to determine whether YAP1 play a role in AAA process by regulating AF function. We found the expression of YAP1was significantly increased in aneurysm tissues of AAA patients compared to normal adjacent vascular tissues and mainly in adventitia. YAP1 also upregulated in elastase-induced and CaCl<sub>2</sub>-induced mice AAA model. Suppressed YAP1 function with YAP1 inhibitor-Verteporfin declined AAA incident rate remarkably in mice, and the collagen deposition in the adventitia was alleviated obviously. Afterwards, we studied the effect of YAP1 on the function of AF, Verteporfin was used to block YAP1 in vitro, the process of AF transforming to myofibroblast and migration were almost completely eliminated after inhibiting YAP1 expression. This study demonstrated that YAP1 may play a key role in AAA development, inhibiting YAP1 significantly reduced AAA formation through suppressed the process of AF transformed to myofibroblast and migration.</p>","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70159","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Up-regulation of HOXA-AS2 and MEG3 long non-coding RNAs acts as a potential peripheral biomarker for bipolar disorder HOXA-AS2和MEG3长非编码RNA的上调可作为双相情感障碍的潜在外周生物标志物。
IF 5.3
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2024-11-01 DOI: 10.1111/jcmm.70150
Maryam Hosseini, Mohammad Javad Mokhtari
{"title":"Up-regulation of HOXA-AS2 and MEG3 long non-coding RNAs acts as a potential peripheral biomarker for bipolar disorder","authors":"Maryam Hosseini,&nbsp;Mohammad Javad Mokhtari","doi":"10.1111/jcmm.70150","DOIUrl":"10.1111/jcmm.70150","url":null,"abstract":"<p>Bipolar disorder (BD) is a psychiatric condition that is frequently misdiagnosed and linked to inadequate treatment. Long non-coding RNAs (lncRNAs) have lately gained recognition as crucial genetic elements and are now regarded as regulatory mechanisms in the neurological system. Our objective was to measure the quantities of HOXA-AS2 and MEG3 ncRNA transcripts. HOXA-AS2 and MEG3 ncRNA levels were checked in the peripheral blood of 50 type I BD and 50 control samples by real-time PCR. Furthermore, we conducted ROC curve analysis and correlation analysis to examine the association between gene expression and specific clinical characteristics in instances with BD. Additionally, a computational study was performed to investigate the binding sites of miRNAs on the HOXA-AS2 and MEG3 lncRNAs. BD subjects showed a significant increase in the expression of HOXA-AS2 and MEG3 compared to controls. The lncRNAs HOXA-AS2 and MEG3 have an area under the ROC curve (AUC) values of 0.70 and 0.71, respectively. There was a significant correlation between the expression levels of ncRNAs HOXA-AS2 and MEG3 in the peripheral blood of patients with BD and occupation scores. The data presented indicate a potential correlation between the expression of HOXA-AS2 and MEG3 lncRNAs with an elevated risk of BD. Furthermore, these lncRNAs may be linked to several molecular pathways. Our findings indicate that the amounts of lncRNAs HOXA-AS2 and MEG3 in transcripts might be a promising potential biomarker for patients with BD.</p>","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70150","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to “The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRα and PPARα” 更正:"源自海洋的呋喃酮通过靶向 LXRα 和 PPARα 减少体外细胞内脂质积累"。
IF 5.3
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2024-11-01 DOI: 10.1111/jcmm.70108
{"title":"Correction to “The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRα and PPARα”","authors":"","doi":"10.1111/jcmm.70108","DOIUrl":"10.1111/jcmm.70108","url":null,"abstract":"<p>Li T, Hu SM, Pang XY, et al. The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRα and PPARα. <i>J Cell Mol Med</i>. 2020;24:3384-3398. doi:10.1111/JCMM.15012</p><p>In Ting Li et al., several incorrect images were used in Figure 3B. The correct figure is shown below. The related fluorescent images were shown in the Supporting Information. The authors confirm all results and conclusions of this article remain unchanged.</p>","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
NAT10-mediated RNA ac4C acetylation contributes to the myocardial infarction-induced cardiac fibrosis NAT10 介导的 RNA ac4C 乙酰化有助于心肌梗死诱发的心脏纤维化。
IF 5.3
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2024-11-01 DOI: 10.1111/jcmm.70141
Jun Li, Feierkaiti Yushanjiang, Zhao Fang, Wan-li Liu
{"title":"NAT10-mediated RNA ac4C acetylation contributes to the myocardial infarction-induced cardiac fibrosis","authors":"Jun Li,&nbsp;Feierkaiti Yushanjiang,&nbsp;Zhao Fang,&nbsp;Wan-li Liu","doi":"10.1111/jcmm.70141","DOIUrl":"10.1111/jcmm.70141","url":null,"abstract":"<p>Cardiac fibrosis is featured cardiac fibroblast activation and extracellular matrix accumulation. Ac4C acetylation is an important epigenetic regulation of RNAs that has been recently discovered, and it is solely carried out by NAT10, the exclusive enzyme used for the modification. However, the potential regulatory mechanisms of ac4C acetylation in myocardial fibrosis following myocardial infarction remain poorly understood. In our study, we activated fibroblasts in vitro using TGF-β1 (20 ng/mL), followed by establishing a myocardial infarction mouse model to evaluate the impact of NAT10 on collagen synthesis and cardiac fibroblast proliferation. We utilized a NAT10 inhibitor, Remodelin, to attenuate the acetylation capacity of NAT10. In the cardiac fibrosis tissues of chronic myocardial infarction mice and cultured cardiac fibroblasts (CFs) in response to TGF-β1 treatment, there was an elevation in the levels of NAT10 expression. This increase facilitated proliferation, the accumulation of collagens, as well as fibroblast-to-myofibroblast transition. Through the administration of Remodelin, we effectively reduced cardiac fibrosis in myocardial infarction mice by inhibiting NAT10's ability to acetylate mRNA. Inhibition of NAT10 resulted in changes in collagen-related gene expression and ac4C acetylation levels. Mechanistically, we found that NAT10 upregulates the acetylation modification of BCL-XL mRNA and enhances the stability of BCL-XL mRNA, thereby upregulating its protein expression, inhibiting the activation of Caspase3 and blocking the apoptosis of CFs. Therefore, the crucial involvement of NAT10-mediated ac4C acetylation is significant in the cardiac fibrosis progression, affording promising molecular targets for the treatment of fibrosis and relevant cardiac diseases.</p>","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70141","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Causal effect of gut microbiota on juvenile idiopathic arthritis: A two-sample Mendelian a randomization study 肠道微生物群对幼年特发性关节炎的因果效应:孟德尔随机双样本研究
IF 5.3
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2024-10-30 DOI: 10.1111/jcmm.70183
Lian Zhang, Zhihua Yang, LuLu Zhang, Yanwen Wei, Lisheng Wan
{"title":"Causal effect of gut microbiota on juvenile idiopathic arthritis: A two-sample Mendelian a randomization study","authors":"Lian Zhang,&nbsp;Zhihua Yang,&nbsp;LuLu Zhang,&nbsp;Yanwen Wei,&nbsp;Lisheng Wan","doi":"10.1111/jcmm.70183","DOIUrl":"https://doi.org/10.1111/jcmm.70183","url":null,"abstract":"<p>There is increasing evidence of a significant association between the gut microbiome and juvenile idiopathic arthritis (JIA). However, whether this association is causal remains to be determined. This study was a two-sample Mendelian randomization (MR) study using publicly available genome-wide association study (GWAS) summary data to investigate the causal relationship between the gut microbiome and JIA. We used summary data on gut flora and JIA obtained from genome-wide association studies (GWAS) from MiBioGen and NHGRI-EBI, using inverse variance weighting as the main method to analyse causality in the TSMR causality analysis. To check the stability of the TSMR results, we performed several sensitivity analyses and assessed the presence of reverse causality through a reverse TSMR analysis. We calculated the degree of sample overlap where applicable. The current TSMR analyses identified four bacterial taxa associated with JIA. Specifically, two bacteria, Catenibacterium (<i>p</i> = 2 × 10–2) and Holdemania (<i>p</i> = 4 × 10–2), were negatively associated with the risk of developing JIA, suggesting a protective effect, while Olsenella (<i>p</i> = 1 × 10–2) and Rikenellaceae (RC9gutgroup) (<i>p</i> = 1 × 10–2) were positively associated with the risk of JIA, suggesting that these two bacteria may be risk factors for JIA. However, the results for Catenibacterium and Holdemania should be interpreted with caution due to instability observed in ‘leave-one-out’ sensitivity analyses. Reverse TSMR analyses found no evidence of reverse causality between JIA and gut flora. Our confirmation of a causal relationship between gut flora and JIA provides an innovative perspective for the study of JIA: targeting and modulating dysregulation of specific bacterial taxa to prevent and treat JIA.</p>","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70183","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142541004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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