JOURNAL OF CELLULAR AND MOLECULAR MEDICINE最新文献_第10页

Comprehensive Multi-Omics Analysis of Copper Metabolism Related Molecular Subtypes and Prognostic Risk Stratification in Colon Adenocarcinoma 结肠腺癌铜代谢相关分子亚型和预后风险分层的综合多组学分析

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-05-20 DOI: 10.1111/jcmm.70591

Xi Sun, Jingfei Tong, Xiaojie Fang, Miaojiong Lu, Chunhui Rao, Yanyan Li

{"title":"Comprehensive Multi-Omics Analysis of Copper Metabolism Related Molecular Subtypes and Prognostic Risk Stratification in Colon Adenocarcinoma","authors":"Xi Sun, Jingfei Tong, Xiaojie Fang, Miaojiong Lu, Chunhui Rao, Yanyan Li","doi":"10.1111/jcmm.70591","DOIUrl":"https://doi.org/10.1111/jcmm.70591","url":null,"abstract":"Colon adenocarcinoma (COAD) is the most common subtype of colorectal cancer, originating from glandular cells in the colon. Despite diagnostic and therapeutic advances, its prognosis remains poor. Copper, an essential micronutrient, is involved in tumorigenesis and other biological processes. In this study, we identified copper metabolism-related genes (CMRG) associated with COAD prognosis from TCGA and GEO databases and constructed a CMRG-based risk model. We assessed its clinical relevance through analyses of immune infiltration, immunotherapy response, and drug sensitivity. Single-cell sequencing revealed the spatial and cellular distribution of CMRG in COAD tissues, providing insight into their roles in the tumour microenvironment. COX19 was selected for further validation, and in vitro experiments (western blot, PCR, siRNA, colony formation, and Transwell assays) confirmed its role in promoting COAD cell invasion and proliferation. These findings highlight the involvement of copper metabolism in COAD progression and suggest potential targets for therapy.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70591","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erk1/2 Orchestrates SSPH I-Induced Oxidative Stress, Mitochondrial Dysfunction and Ferroptosis in Hepatocellular Carcinoma Erk1/2在肝细胞癌中协调SSPH i诱导的氧化应激、线粒体功能障碍和铁凋亡

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-05-20 DOI: 10.1111/jcmm.70609

Yuewen Sun, Ying Zhou, Dan Huang, Zhiguang Zhao, Qingrui Shao, Jianzhe Li, Xiaofang Zhao, Xudong Liu

{"title":"Erk1/2 Orchestrates SSPH I-Induced Oxidative Stress, Mitochondrial Dysfunction and Ferroptosis in Hepatocellular Carcinoma","authors":"Yuewen Sun, Ying Zhou, Dan Huang, Zhiguang Zhao, Qingrui Shao, Jianzhe Li, Xiaofang Zhao, Xudong Liu","doi":"10.1111/jcmm.70609","DOIUrl":"https://doi.org/10.1111/jcmm.70609","url":null,"abstract":"Although Erk1/2 has been linked to oxidative stress regulation in hepatocellular carcinoma (HCC), the interplay among Erk1/2, reactive oxygen species (ROS), and iron metabolism remains poorly characterised. The steroidal saponin SSPH I, a recognised ferroptosis inducer, exerts dual pharmacological effects via Erk1/2 and ROS-dependent pathways. This study aimed to investigate the regulatory mechanisms of Erk1/2 in ferroptosis and oxidative stress and analyse their feedback regulatory effects on Erk1/2 in HCC using SSPH I as a pharmacological probe, and further elucidate the anti-HCC effects and mechanisms of SSPH I in vitro and in vivo. Mechanistic studies utilised three inhibitors: U0126 (Erk1/2 phosphorylation inhibitor), Ferrostatin-1 (ferroptosis inhibitor), and N-acetyl cysteine (ROS scavenger), combined with SSPH I to delineate its effects on cell viability, mitochondrial dynamics, ferroptosis induction and oxidative stress. Mechanistically, SSPH I disrupted mitochondrial function and suppressed HCC cell survival through iron accumulation and ROS generation, while concurrently activating Erk1/2 signalling. Pharmacological inhibition of ROS or iron pathways partially attenuated SSPH I-induced ferroptosis and ROS generation, but failed to abrogate these effects. Erk1/2 inhibition completely abolished SSPH I-mediated regulation of the Nrf1/2-HO-1 axis and ferroptosis-related protein expression in cellular and animal models, identifying Erk1/2 as the upstream regulatory node. Notably, while both SSPH I and U0126 monotherapies inhibited xenograft growth, their combined use resulted in antagonistic effects. These findings establish Erk1/2 activation as the central molecular mechanism orchestrating SSPH I-driven oxidative stress amplification, mitochondrial dysfunction and ferroptosis execution in HCC.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70609","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144100528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Different Doses of Glucosamine Hydrochloride on Cartilage Tissue and Levels of Joint Injury Markers in Knee Osteoarthritis 不同剂量盐酸氨基葡萄糖对膝关节骨性关节炎软骨组织及关节损伤标志物水平的影响

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-05-19 DOI: 10.1111/jcmm.70579

Xichun Wang, Bin Hu, Yi Cheng, Wenjie Chen, Muzi Liu

{"title":"Effects of Different Doses of Glucosamine Hydrochloride on Cartilage Tissue and Levels of Joint Injury Markers in Knee Osteoarthritis","authors":"Xichun Wang, Bin Hu, Yi Cheng, Wenjie Chen, Muzi Liu","doi":"10.1111/jcmm.70579","DOIUrl":"https://doi.org/10.1111/jcmm.70579","url":null,"abstract":"This study analysed the effects of different doses of glucosamine hydrochloride (GS-HCl) on cartilage tissue and the levels of joint injury markers in knee osteoarthritis (KOA). The Sham group, KOA group, low-dose GS-HCl group and high-dose GS-HCl group were established, with six mice in each group. The levels of joint injury markers (COMP, CS846 and CTX-II), inflammatory cytokines (IL-6, TNF-α and iNOS), oxidative stress indicators (MDA and SOD) and matrix remodelling proteins (MMP-3 and TIMP-1) were analysed. The degeneration of knee femoral condyles, histopathological changes and tissue apoptosis rate of the articular cartilage was also assessed. Mice in the KOA group displayed elevated COMP, CS846, CTX-II, IL-6, TNF-α, iNOS and MDA contents, reduced SOD activity, an irregular articular cartilage surface, a serious cartilage defect, a disordered articular cartilage surface in the defect, disappeared cartilage cells, obvious synovial cell proliferation and visible inflammatory cell infiltration. In the tissue, apoptosis rate and MMP-3 and TIMP-1 protein expression increased. Different doses of GS-HCl treatment could reduce COMP, CS846, CTX-II, IL-6, TNF-α, iNOS and MDA contents, apoptosis rate and MMP-3 and TIMP-1 protein expression, increase SOD activity and improve histopathological conditions in KOA mice. The improvement effects in each indicator in the high dose–GS-HCl group were more significant than those in the low dose–GS-HCl group. The intragastric administration of the GS-HCl group partially prevents the degeneration of articular cartilage in KOA mice. The mechanism may be to reduce inflammatory factors and oxidative stress indicator expression and matrix degradation, thereby delaying osteoarthritis progression.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70579","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miRNA Differential Expression Profile Analysis and Identification of Potential Key Genes in Active Tuberculosis 活动性肺结核miRNA差异表达谱分析及潜在关键基因鉴定

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-05-19 DOI: 10.1111/jcmm.70567

Jun Yang, Hanliang Dan, Yeng Chen, Linrong Zou, Shanshan Liu, Feng Wang, Maslinda Binti Musa

{"title":"miRNA Differential Expression Profile Analysis and Identification of Potential Key Genes in Active Tuberculosis","authors":"Jun Yang, Hanliang Dan, Yeng Chen, Linrong Zou, Shanshan Liu, Feng Wang, Maslinda Binti Musa","doi":"10.1111/jcmm.70567","DOIUrl":"https://doi.org/10.1111/jcmm.70567","url":null,"abstract":"Tuberculosis (TB), caused by Mycobacterium TB (MTB), remains a significant global health issue, particularly in developing nations. MicroRNAs (miRNAs) are non-coding RNAs (ncRNAs) that modulate immune responses and play a pivotal role in the pathogenesis of MTB by altering host immune defences. Insights into the regulatory functions of these miRNAs have revealed mechanisms through which MTB evades immune surveillance and establishes persistent infections, highlighting the critical role of miRNA networks in TB pathogenesis. The purpose of this study was to analyse miRNA expression in plasma from TB patients, to predict target genes, and to construct regulatory networks to elucidate the roles of miRNAs in TB pathogenesis. Plasma samples from three patients with active TB and three healthy controls were analysed using high-throughput small RNA sequencing. DEMs were identified using DESeq2, and target genes were predicted via TargetScan and miRWalk. Protein–protein interaction (PPI) networks were constructed using STRING and Cytoscape. Functional enrichment analyses were performed using Gene Ontology (GO) and KEGG databases. A total of 23 DEMs were identified, including 17 upregulated and 6 downregulated miRNAs. hsa-miR-15a-5p emerged as the most significantly upregulated miRNA. PPI network analysis highlighted CCND1, CDK6 and CCND2 as central genes, potentially regulated by miR-15a-5p. GO and KEGG analyses revealed enrichment in pathways related to cell cycle regulation, kinase activity and protein complex formation, suggesting their involvement in TB pathogenesis. This study identifies hsa-miR-15a-5p and its target genes as key components in the regulatory landscape of TB. These findings offer new insights into the molecular mechanisms of TB and propose potential biomarkers and therapeutic targets for future research.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70567","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leukaemia Stem Cells and Their Normal Stem Cell Counterparts Are Morphologically Distinguishable by Artificial Intelligence 白血病干细胞和它们的正常同类干细胞在形态上可以通过人工智能区分

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-05-19 DOI: 10.1111/jcmm.70564

Dongguang Li, Ngoc DeSouza, Kathy Nguyen, Shaoguang Li

{"title":"Leukaemia Stem Cells and Their Normal Stem Cell Counterparts Are Morphologically Distinguishable by Artificial Intelligence","authors":"Dongguang Li, Ngoc DeSouza, Kathy Nguyen, Shaoguang Li","doi":"10.1111/jcmm.70564","DOIUrl":"https://doi.org/10.1111/jcmm.70564","url":null,"abstract":"Leukaemia stem cells (LSCs) are a rare population among the bulk of leukaemia cells and are responsible for disease initiation, progression/relapse and insensitivity to therapies in numerous haematologic malignancies. Identification of LSCs and monitoring of their quantity before, during, and after treatments will provide a guidance for choosing a correct treatment and assessing therapy response and disease prognosis, but such a method is still lacking simply because there are no distinct morphological features recognisable for distinguishing LSCs from normal stem cell counterparts. Using artificial intelligence (AI) deep learning and polycythemia vera (PV) as a disease model (a type of human myeloproliferative neoplasms derived from a haematopoietic stem cell harbouring the JAK2V617F oncogene), we combine 19 convolutional neural networks as a whole to build AI models for analysing single-cell images, allowing for distinguishing between LSCs from JAK2V617F knock-in mice and normal stem counterparts from healthy mice with a high accuracy (> 99%). We prove the concept that LSCs possess unique morphological features compared to their normal stem cell counterparts, and AI, but not microscopic visualisation by pathologists, can extract and identify these features. In addition, we show that LSCs and other cell lineages in PV mice are also distinguishable by AI. Our study opens up a potential AI morphology field for identifying various primitive leukaemia cells, especially including LSCs, to help assess therapy responses and disease prognosis in the future.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70564","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Review on LncRNAs/miRNAs-DNMT1 Axis in Human Cancer: Mechanistic and Clinical Application LncRNAs/miRNAs-DNMT1轴在人类癌症中的作用机制及临床应用综述

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-05-19 DOI: 10.1111/jcmm.70604

Seyed Mohsen Aghaei-Zarch, Ali Esmaeili, Saeid Bagheri-Mohammadi

{"title":"A Comprehensive Review on LncRNAs/miRNAs-DNMT1 Axis in Human Cancer: Mechanistic and Clinical Application","authors":"Seyed Mohsen Aghaei-Zarch, Ali Esmaeili, Saeid Bagheri-Mohammadi","doi":"10.1111/jcmm.70604","DOIUrl":"https://doi.org/10.1111/jcmm.70604","url":null,"abstract":"Cancer constitutes a significant public health concern, and addressing the challenge of cancer holds paramount importance and requires immediate attention. Epigenetic alterations, encompassing DNA methylation, have emerged as pivotal contributors to the development of diverse cancer types. These modifications exert their influence by modulating chromatin structure, gene expression patterns and other nuclear processes, thereby influencing cancer pathogenesis. Over the last two decades, an increasing body of evidence has established the involvement of DNA methyltransferase 1 (DNMT1) in various aspects of cancer development, including tumorigenesis, aggressiveness and treatment response. Furthermore, non-coding RNAs (ncRNAs), such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), are increasingly recognised as significant modulators in diverse biological processes, encompassing metastasis, apoptosis, cell proliferation and differentiation. Several recent studies have elucidated the intricate relationship between epigenetic machinery, specifically DNMT1, and the expression of ncRNAs in the context of cancer. In this review, we provide a comprehensive overview of the interaction between DNMT1 and ncRNAs in cancer pathogenesis. Furthermore, we discuss the important role of the ncRNAs-DNMT1 axis in cancer stem cells and cancer therapy resistance as critical issues in cancer therapy. Finally, we demonstrate that herbal medicine and synthetic RNA molecules regulate DNMT1 activity and hold great promise in cancer treatment.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70604","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “LIPH Promotes Metastasis by Enriching Stem-Like Cells in Triple Negative Breast Cancer” 更正“LIPH通过富集三阴性乳腺癌的干细胞促进转移”

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-05-19 DOI: 10.1111/jcmm.70587

引用次数: 0

Ephedrine Attenuates LPS-Induced Acute Lung Injury in Mice by Inhibiting OTUB1 and Promoting K48 Ubiquitination of HIF1α 麻黄碱通过抑制OTUB1和促进HIF1α K48泛素化减轻lps诱导的小鼠急性肺损伤

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-05-19 DOI: 10.1111/jcmm.70598

Bo Zhou, Keke Zhao, Jiahui Xue, Fangling Zhou, Jin-ao Duan, Yang Niu, Hanqing Wang

{"title":"Ephedrine Attenuates LPS-Induced Acute Lung Injury in Mice by Inhibiting OTUB1 and Promoting K48 Ubiquitination of HIF1α","authors":"Bo Zhou, Keke Zhao, Jiahui Xue, Fangling Zhou, Jin-ao Duan, Yang Niu, Hanqing Wang","doi":"10.1111/jcmm.70598","DOIUrl":"https://doi.org/10.1111/jcmm.70598","url":null,"abstract":"Acute lung injury (ALI) is a severe inflammatory lung disorder that requires effective therapeutic strategies. Ephedrine (EPH) is the main active component found in medicinal plants of the Ephedra genus and is commonly used to modulate inflammatory responses in various diseases. Hypoxia-inducible factor 1-alpha (HIF1α) is a subunit of hypoxia-inducible factor 1 (HIF1), which plays a critical regulatory role in cellular responses under hypoxic conditions. Moreover, the degradation pathway of HIF1α is regulated by the deubiquitinase Ovarian Tumour Domain-containing Ubiquitin Aldehyde Binding Protein 1 (OTUB1). The aim of this study is to investigate the therapeutic effects of EPH on ALI and its potential therapeutic mechanism. We utilised a lipopolysaccharide (LPS)-induced ALI mouse model and employed various methods for evaluation. Ultimately, our research findings demonstrate that EPH exhibits anti-ALI effects, with the involvement of HIF1α and OTUB1 in the pharmacological actions of EPH. In conclusion, our study results demonstrate that EPH exhibits anti-ALI effects and exerts its protective effects through modulation of the OTUB1 and HIF1α pathways. Our research findings not only lay the foundation for expanding the medicinal applications of EPH but also provide direction for seeking improved treatment strategies for ALI.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70598","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnolin Mitigates Skin Ageing Through the CXCL10/p38 Signalling Pathway Magnolin通过CXCL10/p38信号通路缓解皮肤老化

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-05-19 DOI: 10.1111/jcmm.70507

Cheng Wang, Xiaoyun Hu, Tianlin Song, Fan Hu, Le Du, Chenqiong Yan, Tianwei Shen, Nihong Li, Wei Yang, Li Li, Nian Deng, Xingwu Jiang, Yelin Wu, Rui Ye

{"title":"Magnolin Mitigates Skin Ageing Through the CXCL10/p38 Signalling Pathway","authors":"Cheng Wang, Xiaoyun Hu, Tianlin Song, Fan Hu, Le Du, Chenqiong Yan, Tianwei Shen, Nihong Li, Wei Yang, Li Li, Nian Deng, Xingwu Jiang, Yelin Wu, Rui Ye","doi":"10.1111/jcmm.70507","DOIUrl":"https://doi.org/10.1111/jcmm.70507","url":null,"abstract":"Skin ageing accelerates systemic ageing and impairs the body's disease resistance. Inflammaging is a significant contributor to skin ageing. However, the mechanism by which inflammatory factors contribute to skin ageing remains unclear. We conducted screenings and identified CXC motif chemokine ligand 10 (CXCL10) as an inducer of skin ageing, which can be effectively inhibited by magnolin extracted from Magnolia biondii flower. Our results show that CXCL10 not only enhances beta-galactosidase (β-gal) activity but also upregulates the expression of p21/p16, along with matrix metalloproteinases (MMP1, MMP9 and MMP10), through activation of the C-X-C motif chemokine receptor 3 (CXCR3) in human primary fibroblasts. These findings suggest that CXCL10 drives skin ageing by inducing cellular senescence and extracellular matrix degradation. Importantly, treatment with magnolin significantly mitigates skin ageing phenotypes induced by CXCL10 via the p38 signalling pathway. Furthermore, our study demonstrates that magnolin significantly mitigates UV-induced skin ageing in ex vivo human skin samples as well as on human facial skin. This study provides insight into the role of chemokine CXCL10 in promoting inflammaging and proposes an innovative approach for preventing and treating skin ageing.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70507","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant and Analgesic Effect of Melatonin Involving Sirtuin 1: A Randomised Pilot Clinical Study 褪黑素与Sirtuin 1相关的抗氧化和镇痛作用：一项随机试验临床研究

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-05-19 DOI: 10.1111/jcmm.70605

Serafina Perrone, Silvia Carloni, Serena Benedetti, Micheal Weiss, Lucia Marseglia, Valentina Dell'Orto, Virginia Beretta, Noemi Pappagallo, Marica Pagliarini, Maria Cristina Albertini, Patrizia Ambrogini, Walter Balduini, Angela Simona Montalto, Pietro Impellizzeri, Giuseppe Buonocore, Eloisa Gitto, Carmelo Romeo

{"title":"Antioxidant and Analgesic Effect of Melatonin Involving Sirtuin 1: A Randomised Pilot Clinical Study","authors":"Serafina Perrone, Silvia Carloni, Serena Benedetti, Micheal Weiss, Lucia Marseglia, Valentina Dell'Orto, Virginia Beretta, Noemi Pappagallo, Marica Pagliarini, Maria Cristina Albertini, Patrizia Ambrogini, Walter Balduini, Angela Simona Montalto, Pietro Impellizzeri, Giuseppe Buonocore, Eloisa Gitto, Carmelo Romeo","doi":"10.1111/jcmm.70605","DOIUrl":"https://doi.org/10.1111/jcmm.70605","url":null,"abstract":"Melatonin is a potent antioxidant molecule, and its analgesic effects have been observed in children. However, the underlying mechanisms of these effects have not yet been fully explored in clinical studies. We tested the hypothesis that melatonin reduces pain and oxidative stress involving the sirtuin pathway. Forty-four children were randomly assigned to oral supplementation with melatonin or placebo before induction of anaesthesia for surgery. Plasma levels of 4-hydroxynonenal (4-HNE), melatonin, sirtuin 1 (SIRT1) and circulating miR-34 and miR-124a were analysed at T0 (pre-hospitalisation), T1 (before surgery) and T2 (1 h after the end of the surgery). Melatonin decreased 4-HNE and increased SIRT1 concentrations at T2 in supplemented children. Significant correlations were found between melatonin and pain score (R = −0.404), 4-HNE and pain score (R = 0.44), melatonin and 4-HNE (R = 0.42), 4-HNE and SIRT1 (R = −0.43) and melatonin and SIRT1 (R = 0.41) at T2. Circulating miR-34 and miR-124a modulation were also observed. The reduction of oxidative stress and the modulation of circulating miR-34 and miR-124a, which target SIRT1 activity, suggest a novel pathway underlying melatonin's antioxidant and analgesic effects.Trial Registration: ClinicalTrials.gov identifier: NCT06724432","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70605","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0