JOURNAL OF CELLULAR AND MOLECULAR MEDICINE最新文献_第3页

Nomogram Based on A-To-I RNA Editing for Predicting Overall Survival in Patients With Breast Cancer 基于A-To-I RNA编辑的Nomogram预测乳腺癌患者总生存期

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-09-25 DOI: 10.1111/jcmm.70781

Yangyang Zhang, Jia Zhou, Hong Li, Dahai Chai, Bin Lian, Yaobang Liu, Li Guo, Jinping Li

{"title":"Nomogram Based on A-To-I RNA Editing for Predicting Overall Survival in Patients With Breast Cancer","authors":"Yangyang Zhang, Jia Zhou, Hong Li, Dahai Chai, Bin Lian, Yaobang Liu, Li Guo, Jinping Li","doi":"10.1111/jcmm.70781","DOIUrl":"10.1111/jcmm.70781","url":null,"abstract":"Adenosine-to-inosine RNA editing (ATIRE) is the most common type of RNA editing in higher eukaryotes. Many RNA editing events are associated with the occurrence and development of various tumours. Currently, several ATIRE sites have been used as predictors of cancer prognosis. However, whether some of them can be used as diagnostic and prognostic markers for patients with breast cancer (BRCA) remains unknown. BRCA-related data and RNA editing data were downloaded from the TCGA database, and the patients were randomly divided into training (n = 503) and validation (n = 334) groups. Through univariate Cox regression, Lasso regression, and multivariate Cox regression analysis, nine ATIRE sites related to prognosis in all groups were identified to construct a prognostic model and generate an ATIRE risk score. The median survival time of patients with high-risk scores was significantly shortened, and the nomogram performed well in predicting the overall survival time of patients with BRCA. Calibration and decision curves verified the high accuracy of the model. Among them, five ATIRE sites correlated with the expression of the corresponding genes, and the expression of four ATIRE sites in tumour tissues was significantly higher than that in normal tissues (p < 0.05). Reverse transcription quantitative polymerase chain reaction and immunohistochemical staining experiments were used for preliminary experimental validation of the results. The prognostic model based on ATIRE could serve as a new tool for predicting the survival and prognosis of patients with BRCA and help clinicians provide better individualised clinical decision-making.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 18","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70781","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative Single-Cell and Machine Learning Analysis Reveals Immune Microenvironment Remodelling in Lymph Node Metastasis of Lung Adenocarcinoma 综合单细胞和机器学习分析揭示肺腺癌淋巴结转移的免疫微环境重塑。

IF 4.2

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-09-23 DOI: 10.1111/jcmm.70859

Shuai Jiang, Jinhan Zhao, Jiaqi Feng, Yue Yu, Lianmin Zhang, Chenjun Huang, Zhenfa Zhang

{"title":"Integrative Single-Cell and Machine Learning Analysis Reveals Immune Microenvironment Remodelling in Lymph Node Metastasis of Lung Adenocarcinoma","authors":"Shuai Jiang, Jinhan Zhao, Jiaqi Feng, Yue Yu, Lianmin Zhang, Chenjun Huang, Zhenfa Zhang","doi":"10.1111/jcmm.70859","DOIUrl":"10.1111/jcmm.70859","url":null,"abstract":"Lymph node metastasis is a pivotal determinant of prognosis in lung adenocarcinoma, yet its impact on tumour microenvironment remodelling remains insufficiently characterised. In this study, we employed single-cell RNA sequencing to compare metastatic and non-metastatic lymph nodes, delineating metastasis-associated immune and stromal alterations. Metastatic nodes exhibited marked reductions in dendritic cell and T cell infiltration alongside increases in monocytes and SPP1+ macrophages, indicative of an immunosuppressive milieu. Intercellular communication analysis revealed strengthened interactions among SPP1+ macrophages, monocytes, and epithelial cells, suggesting coordinated signalling that may further enforce immune suppression. Integrating differentially expressed genes with multi-omic features, we developed an ensemble machine learning model, LNRScore, which robustly stratified patients into distinct risk groups. A high LNRScore was associated with poorer prognosis and reduced immune infiltration, whereas a low LNRScore correlated with higher immunogenicity and greater predicted responsiveness to immunotherapy based on TCIA assessments. Further analyses identified HMGA1 as a core gene within the model, closely linked to adverse outcomes; functional assays demonstrated that high HMGA1 expression promotes the proliferation and migration of the LLC cell line, supporting its role in metastatic progression. Collectively, this study defines the immune microenvironmental remodelling associated with lymph node metastasis, establishes an effective risk prediction model (LNRScore), and highlights HMGA1 as a potential target for precision diagnosis and therapy in lung adenocarcinoma.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 18","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jianpi-Huogu Prescription Repairs Nontraumatic Osteonecrosis of the Femoral Head by Inhibiting NAMPT/STK11/HMGCR/ACAT1 Axis-Mediated Lipid Production 健脾活骨方通过抑制NAMPT/STK11/HMGCR/ACAT1轴介导的脂质生成修复非外伤性股骨头坏死

IF 4.2

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-09-23 DOI: 10.1111/jcmm.70858

Tao Li, Zhaochen Ma, Shuangrong Gao, Chu Zhang, Yan Jia, Huang Feng, Na Lin, Weiheng Chen, Yanqiong Zhang

{"title":"Jianpi-Huogu Prescription Repairs Nontraumatic Osteonecrosis of the Femoral Head by Inhibiting NAMPT/STK11/HMGCR/ACAT1 Axis-Mediated Lipid Production","authors":"Tao Li, Zhaochen Ma, Shuangrong Gao, Chu Zhang, Yan Jia, Huang Feng, Na Lin, Weiheng Chen, Yanqiong Zhang","doi":"10.1111/jcmm.70858","DOIUrl":"10.1111/jcmm.70858","url":null,"abstract":"Jianpi-Huogu Prescription (JHP), integrating the classic Chinese herbal formulas Linggui-Zhugan Decoction and Si-Wu Decoction, has shown clinical efficacy in treating early nontraumatic osteonecrosis of the femoral head (NONFH). In this study, 299 chemical constituents of JHP were identified using ultra-performance liquid chromatography–quadrupole-time of flight mass spectrometry. Pharmacological evaluations in rats with early NONFH demonstrated that JHP attenuated femoral head pathological changes, improved gait parameters, increased mechanical pain thresholds, reduced serum levels of triglycerides, total cholesterol, low-density lipoprotein, very-low-density lipoprotein, and the TXB₂/6-keto-PGF₁α ratio while elevating high-density lipoprotein, decreased serum inflammatory cytokines (IL-1β, IL-6, TNF-α) and the RANKL/OPG ratio, and restored bone trabecular morphology and reduced the number of bone marrow adipocytes. Mechanistically, through transcriptomic profiling, network calculations and experimental validation, JHP downregulated NMNAT1, NMNAT3, and NAMPT in the femoral head, thereby reducing NAD+ synthesis, increased ATP production (evidenced by a decreased ADP/ATP ratio) and upregulated STK11 expression, activated p-HMGCR while suppressing ACAT1 thus inhibiting cholesterol synthesis and lipid accumulation, and surface plasmon resonance confirmed direct binding of its active components (ligustilide, 5,6,4′-trihydroxy-7,3′-dimethoxyflavone, (Z)-3-butylidenephthalide, senkyunolide H, ferulic acid, guanosine) to NMNAT1. In conclusion, JHP ameliorates early NONFH by regulating lipid metabolism, improving hypercoagulability, and restoring bone homeostasis through the NMNAT1/NMNAT3/NAMPT/STK11/HMGCR/ACAT1 axis.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 18","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

USP14 Mediates Molecular Mechanisms Regulating Aortic Valve Stenosis Through Ubiquitination USP14通过泛素化介导主动脉瓣狭窄的分子机制

IF 4.2

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-09-23 DOI: 10.1111/jcmm.70765

Yin Yang, Bo-Chen Yao, Jing-Hui Li, Qing-Liang Chen, Nan Jiang, Lian-Qun Wang, Zhi-Gang Guo

{"title":"USP14 Mediates Molecular Mechanisms Regulating Aortic Valve Stenosis Through Ubiquitination","authors":"Yin Yang, Bo-Chen Yao, Jing-Hui Li, Qing-Liang Chen, Nan Jiang, Lian-Qun Wang, Zhi-Gang Guo","doi":"10.1111/jcmm.70765","DOIUrl":"10.1111/jcmm.70765","url":null,"abstract":"The incidence of aortic valve stenosis (AVS) has been increasing in recent years, making it one of the leading causes of cardiovascular-related deaths among the elderly. Clinical samples from 30 AVS patients and 30 controls treated at Tianjin Chest Hospital between 2010 and 2020 were collected. Analyses included immunofluorescence detection, Western blotting (WB), quantitative RT-PCR analysis (qRT-PCR), haematoxylin and eosin (HE) staining and molecular docking experiments of protein–protein interactions. USP14 was found to be highly expressed in AVS tissues, which was validated by immunofluorescence and WB analyses. qRT-PCR results indicated that the mRNA expression levels of USP14 and CDK4 were significantly elevated in AVS tissues. HE staining revealed significant pathological changes in AVS tissues. Molecular docking experiments demonstrated the interaction between USP14 and CDK4, suggesting a potential regulatory mechanism in AVS. USP14 may be involved in the occurrence and development of AVS by regulating cell proliferation, apoptosis and fibrosis processes. It may serve as a therapeutic target for treating AVS.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 18","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145130990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GCN5L1 Aggravates Postherpetic Neuralgia Through Regulating Microglial Mitochondrial Fission–Fusion Homeostasis GCN5L1通过调节小胶质细胞线粒体分裂融合稳态加重疱疹后神经痛。

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-09-23 DOI: 10.1111/jcmm.70861

Wang Li, Xin Cao, Shenghan Wang, Xuedong Jin, Hongqian Wang

{"title":"GCN5L1 Aggravates Postherpetic Neuralgia Through Regulating Microglial Mitochondrial Fission–Fusion Homeostasis","authors":"Wang Li, Xin Cao, Shenghan Wang, Xuedong Jin, Hongqian Wang","doi":"10.1111/jcmm.70861","DOIUrl":"10.1111/jcmm.70861","url":null,"abstract":"Postherpetic neuralgia (PHN) is a debilitating chronic pain condition following varicella-zoster virus (VZV) reactivation, characterised by persistent neuroinflammation. However, the intracellular mechanisms that drive microglial activation and sustained pain sensitisation remain poorly understood. Due to mice having no VZV infection receptor, herpes simplex virus type 1 (HSV-1) infection is a well-established PHN mice model. Here, we identified GCN5L1, a mitochondrial acetylation modulator, as a critical regulator of microglial mitochondrial dynamics and a key contributor to PHN pathogenesis. We found that GCN5L1 was markedly upregulated in the spinal dorsal horn after PHN, particularly located in microglia. Microglial Gcn5l1 deficiency attenuated HSV-1-induced neuroinflammatory responses and alleviated mechanical allodynia, whereas Gcn5l1 overexpression exacerbated neuroinflammatory responses both in vivo and in vitro. Mechanistically, GCN5L1 promoted mitochondrial fission and impaired oxidative metabolism by enhancing DRP1 acetylation, without altering the expression of canonical fission–fusion regulators. Restoration of mitochondrial fission using MFI8 intrathecally reversed the anti-inflammatory and analgesic effects of Gcn5l1 deficiency, confirming that GCN5L1 mediated pain sensitisation through mitochondrial fission–fusion in PHN. Finally, inhibiting GCN5L1 by AAV-shGCN5L1 intrathecally suppressed neuroinflammation and mechanical allodynia in PHN mice. These findings uncovered that GCN5L1 aggravated neuroinflammation and PHN through regulating microglial mitochondrial fission–fusion homeostasis, offering new insights and potential feasibility in clinical translation for PHN management.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 18","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Classification of Paediatric Celiac Disease Using RNA Sequencing and Real-Time PCR of Duodenal Biomarkers 利用十二指肠生物标志物的RNA测序和实时荧光定量PCR对小儿乳糜泻进行分类

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-09-22 DOI: 10.1111/jcmm.70854

Hanna Gustafsson Bragde, Sven Almer, Jan Söderman

{"title":"Classification of Paediatric Celiac Disease Using RNA Sequencing and Real-Time PCR of Duodenal Biomarkers","authors":"Hanna Gustafsson Bragde, Sven Almer, Jan Söderman","doi":"10.1111/jcmm.70854","DOIUrl":"https://doi.org/10.1111/jcmm.70854","url":null,"abstract":"Celiac disease (CD) diagnosis in children with sub-threshold tissue transglutaminase autoantibody (anti-TG2) levels requires a small intestinal biopsy. Through RNA sequencing and real-time PCR of small intestinal biopsies, gene expression in such children was compared with the expression in children with active CD and anti-TG2 levels above the threshold, and with non-CD children. The study also included CD children with a non-diagnostic first biopsy to explore early gene expression changes in CD. The aim of the study was to explore gene expression in relation to anti-TG2 levels, investigate gene expression in Potential CD, and provide a gene expression profile to aid in CD diagnostics. The results showed that in active CD, expression changes involved genes associated with e.g., immune response, transport, angiogenesis, and epithelial barrier function, with even more pronounced changes of genes associated with cell cycle progression, absorption, lipid and lipoprotein processes, and retinoid metabolism in the active CD group with higher anti-TG2 levels. Gene expression changes in CD children with a non-diagnostic first biopsy showed large inter-individual variations, but in general, gene expressions were associated with many of the same biological contexts as in active CD, including epithelial barrier function. Overall, the results show that gene expression profiling has great potential as a complement to the histopathologic assessment in CD diagnostics, even early in the disease course, but probably cannot be used for prognostic purposes.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 18","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70854","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RETRACTION: Berberine Alleviates Contrast-Induced Nephropathy by Activating Akt/Foxo3a/Nrf2 Signalling Pathway 结论：小檗碱通过激活Akt/Foxo3a/Nrf2信号通路缓解造影剂诱导的肾病

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-09-22 DOI: 10.1111/jcmm.70863

{"title":"RETRACTION: Berberine Alleviates Contrast-Induced Nephropathy by Activating Akt/Foxo3a/Nrf2 Signalling Pathway","authors":"","doi":"10.1111/jcmm.70863","DOIUrl":"https://doi.org/10.1111/jcmm.70863","url":null,"abstract":"\u0000 RETRACTION: W. Wang, R. Yu, C. Wu, Q. Li, J. Chen, Y. Xiao, H. Chen, J. Song, M. Ji, and Z. Zuo, “ Berberine Alleviates Contrast-Induced Nephropathy by Activating Akt/Foxo3a/Nrf2 Signalling Pathway,” Journal of Cellular and Molecular Medicine 28, no. 1 (2024): e18016, https://doi.org/10.1111/jcmm.18016.\u0000 The above article, published online on 01 November 2023 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editor-in-Chief, Stefan Constantinescu; The Foundation for Cellular and Molecular Medicine; and John Wiley and Sons Ltd. The retraction has been agreed upon following an investigation into concerns raised by a third party, regarding the accuracy of the flow cytometry analyses and apoptosis measurements in Figure 5A and 8B, as well as the integrity of the Western blot data in Figure 7 and 8C. The authors provided partial raw data, which revealed methodological issues, including inappropriate flow cytometer settings and incorrect use of the apoptosis detection kit, which renders the published flow cytometry results unsupported. Additionally, errors were identified in the representation of Western blot data in Figures 2B, 7, and 8. The authors fully cooperated with the investigation and repeated the relevant experiments. However, the new data failed to fully support the original conclusions, raised further concerns regarding the reproducibility of the flow cytometry results, and highlighted additional methodological issues in the Western blot analyses. Given the identified issues, the editors have lost confidence in the data presented and consider the conclusions of this manuscript insufficiently supported. The authors did not respond when asked to agree to the final wording of the retraction.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 18","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70863","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic Efficacy of HPβ-CD-Angiotensin-(1-7) Oral Formulation in Muscle Injury Recovery in Rat hp β- cd -血管紧张素-(1-7)口服制剂对大鼠肌肉损伤恢复的治疗作用

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-09-22 DOI: 10.1111/jcmm.70851

Nádia Lúcia Totou, Ana Maria Sampaio Rocha, Samara Silva de Moura, César Henrique Pereira, Fabricio Sampaio Coelho, Douglas Daniel Dophine, Daniel Barbosa Coelho, Emerson Cruz de Oliveira, Robson Augusto Souza dos Santos, Lenice Kappes Becker, Wanderson Geraldo de Lima

{"title":"Therapeutic Efficacy of HPβ-CD-Angiotensin-(1-7) Oral Formulation in Muscle Injury Recovery in Rat","authors":"Nádia Lúcia Totou, Ana Maria Sampaio Rocha, Samara Silva de Moura, César Henrique Pereira, Fabricio Sampaio Coelho, Douglas Daniel Dophine, Daniel Barbosa Coelho, Emerson Cruz de Oliveira, Robson Augusto Souza dos Santos, Lenice Kappes Becker, Wanderson Geraldo de Lima","doi":"10.1111/jcmm.70851","DOIUrl":"https://doi.org/10.1111/jcmm.70851","url":null,"abstract":"To evaluate the therapeutic effect of oral treatment with HPβ-CD-angiotensin-(1-7) (Ang-(1-7)) on muscle recovery after laceration injury. Wistar rats were divided into four groups: Control (n = 10); HPβ-CD-Ang-(1-7) (n = 10); muscle injury + HPβ-CD (MI + Placebo) (n = 24); and muscle injury + HPβ-CD-Ang-(1-7) (MI + Ang-(1-7)) (n = 24). After 7–21 days of treatment, physical performance, histological features and the expression of pro- and anti-fibrotic genes were evaluated. The MI + Ang-(1-7) group showed improved control of the inflammatory phase and reduced deposition of collagen types I and III compared to MI + Placebo. CTGF gene expression analysis revealed lower levels of pro-fibrotic markers and higher expression of proteins involved in blocking fibrotic pathways. In treadmill tests, MI + Ang-(1-7) animals also showed superior physical performance at all evaluated time points. Oral treatment with Ang-(1-7) is effective in promoting recovery from muscle injuries, particularly fibrotic lesions, while preserving muscle function and enhancing physical performance.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 18","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70851","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outcomes of Wild Type and TP53-Mutated B Cell Malignancy Patients Receiving CAR-T Cell Therapy: A Systematic Review and Meta-Analysis 野生型和tp53突变B细胞恶性肿瘤患者接受CAR-T细胞治疗的结果：一项系统综述和荟萃分析

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-09-22 DOI: 10.1111/jcmm.70818

Wenxin Qi, Yuqi Zhang, Xiaoyu Hao, Ping Yang, Jing Wang, Chaoling Wu, Weilong Zhang, Hongmei Jing

{"title":"Outcomes of Wild Type and TP53-Mutated B Cell Malignancy Patients Receiving CAR-T Cell Therapy: A Systematic Review and Meta-Analysis","authors":"Wenxin Qi, Yuqi Zhang, Xiaoyu Hao, Ping Yang, Jing Wang, Chaoling Wu, Weilong Zhang, Hongmei Jing","doi":"10.1111/jcmm.70818","DOIUrl":"https://doi.org/10.1111/jcmm.70818","url":null,"abstract":"P53 mutation (TP53m) is a common intrinsic factor involved in relapsed or refractory (R/R) B cell malignancies that associates with treatment resistance. As a novel immunotherapy, CAR-T has been increasingly applied in TP53m B cell malignancies, yet whether it can overcome the poor outcome of the TP53m population is controversial. We searched MEDLINE and EMBASE to identify population-based cohort studies that evaluated the CAR-T treatment outcomes between wild type and TP53m patients in B cell malignancies. Meta-analysis on their complete response (CR), partial response (PR), overall response rate (ORR), progression-free survival (PFS) and overall survival (OS) was carried out and pooled risk ratios (RR) or hazard ratios (HR) were estimated. A total of 10 eligible studies reporting 848 patients with B cell malignancies from wild type and TP53m groups receiving CAR-T therapy were selected. The CR and ORR were comparable in both wild type and TP53m patients either with B cell lymphoma or leukaemia (all p > 0.05). However, the TP53m group was associated with shorter PFS and OS in both diseases (all p < 0.05). In traditional single targeting CAR-T therapy, the PFS and OS were shorter in the TP53m group than in the wild type group (all p < 0.05). In contrast, the former outcomes of the wild type and TP53m groups were comparable when receiving dual-targeting CAR-T treatment (all p > 0.05). Though the CR and ORR of wild type and TP53m groups were similar, the PFS and OS of B cell malignancy patients bearing TP53m were inferior to wild type patients receiving CAR-T cell treatment. Notably, the CR, PFS and OS of wild type and TP53m groups exhibit the same therapeutic effect via CD19/22 CAR-T cocktail therapy. In other words, the poor prognosis of TP53m patients may be overcome by double targeting CAR-T mode.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 18","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70818","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduced FGF9 Leads to Kidney Injury Through Regulating Renal Tubular Epithelial Cell EMT in Diabetes 糖尿病患者FGF9减少通过调节肾小管上皮细胞EMT导致肾损伤

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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Pub Date : 2025-09-22 DOI: 10.1111/jcmm.70856

Wen-qing Chen, Chengyang Sun, Xiaotan Zhang, Xunjia Ye, Yuzhen Liu, Hui-di Wang, Lichao Liu, Danrui Li, Jingyun Wang, Meiting Shi, Fang Yang, Christoph Reichetzeder, Berthold Hocher, Xuesong Yang, Baozhang Guan, Guang Wang

{"title":"Reduced FGF9 Leads to Kidney Injury Through Regulating Renal Tubular Epithelial Cell EMT in Diabetes","authors":"Wen-qing Chen, Chengyang Sun, Xiaotan Zhang, Xunjia Ye, Yuzhen Liu, Hui-di Wang, Lichao Liu, Danrui Li, Jingyun Wang, Meiting Shi, Fang Yang, Christoph Reichetzeder, Berthold Hocher, Xuesong Yang, Baozhang Guan, Guang Wang","doi":"10.1111/jcmm.70856","DOIUrl":"https://doi.org/10.1111/jcmm.70856","url":null,"abstract":"Diabetic nephropathy (DN) stands out as one of the most prevalent and severe chronic microvascular complications associated with diabetes, serving as the primary cause of end-stage renal disease (ESRD) in developed and developing countries. However, the precise pathogenesis remains incompletely elucidated. Our study suggests FGF9 as a key gene in DN using bioinformatics analysis. We found a negative correlation between FGF9 and serum creatinine and a positive one with glomerular filtration rate in DN patients. FGF9 expression was lower in DN patients' glomeruli and tubules. High FGFR expression in renal tubular cells, along with increased α-SMA and TGF-β1, indicates a role for the epithelial-to-mesenchymal transition (EMT) process in diabetes mellitus (DM) mouse renal tubular epithelial cells. Subsequently, we modulated FGF9 in HK2 cells under different glucose conditions. The genes regulated by FGF9 were identified (LOX, HIF1α, THBS1, TGFβ2 and ITGβ1) through RNA sequencing analysis. It was suggested that FGF9 promotes the development of renal EMT probably through regulating these genes. Overall, FGF9 could be a biomarker and therapeutic target for DN.","PeriodicalId":101321,"journal":{"name":"JOURNAL OF CELLULAR AND MOLECULAR MEDICINE","volume":"29 18","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcmm.70856","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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