Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology最新文献_第9页

Immunological characterization of yersinia enterocolitica O:9 and O:3 LPS antigens by monoclonal antibodies 单克隆抗体对小肠结肠炎耶尔森菌O:9和O:3 LPS抗原的免疫学特性研究

Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology Pub Date : 1988-11-01 DOI: 10.1016/S0176-6724(88)80173-1

Jürgen Weninger, Klaus Wartenberg, Martin Röllinghoff

{"title":"Immunological characterization of yersinia enterocolitica O:9 and O:3 LPS antigens by monoclonal antibodies","authors":"Jürgen Weninger, Klaus Wartenberg, Martin Röllinghoff","doi":"10.1016/S0176-6724(88)80173-1","DOIUrl":"10.1016/S0176-6724(88)80173-1","url":null,"abstract":"<div><p>LPS-specific monoclonal antibodies induced against <em>Y. enterocolitica</em> serotype O:9 bacteria and against <em>Y. enterocolitica</em> O:3 were used in a comparative study to characterize the O:9 and O:3 LPS. <em>Yersinia</em> bacteria grown at 22 °C and 37°C and LPS preparations thereof were tested. SDS-PAGE, Western blot analysis and adsorption procedures revealed that LPS of <em>Y. enterocolitica</em> O:9 differed from that of <em>Y. enterocolitica</em> O:3 in: (i) its repeating LPS O-side-chain sugar, (ii) its shorter length of the LPS O-side-chain and (iii) its failure to show temperature-dependent variation in the length of O-side-chains.</p></div><div><p>Zu einer vergleichenden immunologischen Charakterisierung von <em>Y. enterocolitica</em> Serotyp O:9 und O:3 LPS wurden entsprechende monoklonale Antikörper eingesetzt. Als Antigen wurden bei 22 °C und 37°C gewachsene intakte Yersinien, sowie LPS-Präparationen von diesen verwendet. SDS-PAGE, Western-Blot-Analysen und Adsorptionsexperimente zeigten, daß sich das LPS von <em>Y. enterocolitica</em> O:9 von dem von <em>Y. enterocolitica</em> O:3 in (a) seinem LPS O-Seitenkettenzucker, (b) in der Länge der O-Seitenketten und (c) in einer temperaturunabhängigen Länge der O-Seitenketten unterscheidet.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"269 3","pages":"Pages 298-313"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80173-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14347370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Lectin-Mediated Cell-Attachment and Phagocytosis of Staphylococcus Saprophyticus Strain S1 腐生葡萄球菌S1株凝集素介导的细胞附着和吞噬作用

Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology Pub Date : 1988-11-01 DOI: 10.1016/S0176-6724(88)80137-8

J. Beuth , H.L. Ko, Y. Ohshima, F. Schumacher-Perdreau, G. Peters, G. Pulverer

{"title":"Lectin-Mediated Cell-Attachment and Phagocytosis of Staphylococcus Saprophyticus Strain S1","authors":"J. Beuth , H.L. Ko, Y. Ohshima, F. Schumacher-Perdreau, G. Peters, G. Pulverer","doi":"10.1016/S0176-6724(88)80137-8","DOIUrl":"10.1016/S0176-6724(88)80137-8","url":null,"abstract":"<div><p>Adhesion studies with cryotome sections of human kidney and lung respectively uroepithelial cells together with blocking experiments with competitive carbohydrates suggested that specific attachment of <em>S. saprophyticus</em> strain S 1 to host cells apparently is mediated by lectins. Accordingly, microbial lectin blocking with specific glycoconjugates or lectin dysfunction (after treatment of bacteria with subinhibitory concentrations of tunicamycin) significantly decreased staphylococcal adherence to epithelial cells. Chemiluminescence measurements of human polymorphonuclear leukocyte (PMN) function yielded results suggesting importance of lectin-receptor interaction in phagocytosis, too, since PMN activity was significantly decreased after staphylococcal lectin blocking or dysfunction.</p></div><div><p>Adhäsions- und Inhibitionsstudien mit Kryotomschnitten menschlicher Niere und Lunge bzw. mit Uroepithelzellen demonstrierten, daß die spezifische Adhäsion von <em>S. saprophyticus</em> Stamm S 1 an Wirtszellen offensichtlich durch bakterielle Lektine vermittelt wird. Lektinblockade durch rezeptorkomplementäre Kohlenhydrate bzw. Funktionseinschränkung der Lektine (nach Inkubation der Mikroorganismen in subinhibitorischen Konzentrationen des Antibiotikums Tunicamycin) bewirkte eine signifikant reduzierte Adhäsion von <em>S. saprophyticus</em> an die Epithelzellen. Chemilumineszenz-Messungen zeigten, daß Rezeptor-Ligand-Wechselwirkungen auch für den Prozeß der Granulozytenstimulation von Bedeutung sind. Blockade der Staphylokokken-Lektine sowie deren Funktionseinbuße (nach Tuni-camycininkubation) schränkte die Aktivierbarkeit von Granulozyten signifikant ein.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 22-27"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80137-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14350444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Dependence of virus adsorption to the cell surface on the input multiplicity of infection 病毒在细胞表面的吸附依赖于感染的输入多重性

Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology Pub Date : 1988-11-01 DOI: 10.1016/S0176-6724(88)80072-5

M. Lipkind , V. Urbakh

引用次数: 1

Immune-Mediated Pathogenesis of Borna Disease 博纳病的免疫介导发病机制

Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology Pub Date : 1988-11-01 DOI: 10.1016/S0176-6724(88)80166-4

R. Rott , S. Herzog, J. Richt, L. Stitz

{"title":"Immune-Mediated Pathogenesis of Borna Disease","authors":"R. Rott , S. Herzog, J. Richt, L. Stitz","doi":"10.1016/S0176-6724(88)80166-4","DOIUrl":"10.1016/S0176-6724(88)80166-4","url":null,"abstract":"<div><p>Borna disease is an endemic progressive encephalomyelitis of horses and sheep prevalent in central Europe. A wide variety of animal species, ranging from chickens to primates can be infected experimentally with the causative virus, which is only poorly characterized. Furthermore, BD virus-specific antibodies have been detected in sera and cerebrospinal fluids of psychiatric patients.</p><p>Our studies on the pathogenesis of BD have shown that — at least in rats — the disease is not caused by the infecting virus itself, but by a virus-induced immunopathological reaction. Thus, after intracerebral infection immunoincompetent rats do not get the disease despite persistent virus replication in cells of the central nervous system. However, after adoptive transfer of immune cells from diseased rats, immunoincompetent rats exhibit full-blown BD. Recently, we have been successful in establishing a virus-specific T cell line of the helper/inducer phenotype (CD4<sup>+</sup>). This T cell was shown to play an important role in the pathogenesis of BD, suggesting that the disease is caused by a delayed type hypersensitivity reaction.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 295-301"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80166-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14392945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

II. Sachverzeichnis 2 . .Sachverzeichnis

Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology Pub Date : 1988-11-01 DOI: 10.1016/S0176-6724(88)80076-2

引用次数: 0

Investigations on the Opsonin-Dependent Interaction of Klebsiella Bacteria and Human Polymorphonuclear Leukocytes 克雷伯菌与人多形核白细胞依赖调理素相互作用的研究

Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology Pub Date : 1988-11-01 DOI: 10.1016/S0176-6724(88)80161-5

A. Przondo-Mordarska , H.L. Ko , M. Matej-Melczynska , J. Beuth , W. Roszkowski , G. Pulverer

{"title":"Investigations on the Opsonin-Dependent Interaction of Klebsiella Bacteria and Human Polymorphonuclear Leukocytes","authors":"A. Przondo-Mordarska , H.L. Ko , M. Matej-Melczynska , J. Beuth , W. Roszkowski , G. Pulverer","doi":"10.1016/S0176-6724(88)80161-5","DOIUrl":"10.1016/S0176-6724(88)80161-5","url":null,"abstract":"<div><p>Encapsulated and nonencapsulated <em>Klebsiella</em> strains with different types of fimbriation were tested for their opsonin-dependent stimulation of human polymorphonuclear leukocytes (PMNs). Chemiluminescence (CL) of the PMNs was determined since CL-response can be used indirectly to quantitate phagocytosis. Apparently, the interaction between <em>Klebsiella</em> bacteria and human PMNs is dependent on the type of fimbration and on the presence of capsules. Evaluation of the bactericidal capacity of human PMNs against <em>Klebsiella</em> bacteria positively correlated with the chemiluminescence response demonstrating the importance of fimbriation and capsulation for this process, too.</p></div><div><p>Bekapselte <em>Klebsiellen-Stämme</em> mit unterschiedlichem Fimbrienbesatz (Stämme ohne Fimbrien, Stämme mit Fimbrien des Types 1 oder 3 und Stämme mit Fimbrien des Types 1 und 3) und ihre nichtbekapselten Mutanten wurden auf ihre (opsoninabhängige) stimulatorische Potenz gegenüber menschlichen Granulozyten untersucht. Vorhandensein oder Fehlen von Fimbrien oder Schleimkapsel trugen entscheidend zur <em>Klebsiellen-Granuloiyteninteraktion</em> bei und manifestierten sich eindeutig im Chemilumineszenz-Test. Der Bakterienabtötungseffekt durch menschliche Granulozyten korrelierte positiv mit den Chemilumineszenzstudien. Kapsel und Fimbrien sowie Fimbrienart hatten Einfluß auf den direkten <em>Klebsiellen</em>-Abtötungseffekt durch Granulozyten.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 252-259"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80161-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14042786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Biological and biochemical activities of a toxoid of erythrogenic toxin type A a型红毒素类毒素的生物学和生化活性

Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology Pub Date : 1988-11-01 DOI: 10.1016/S0176-6724(88)80069-5

Heide Knöll , Dieter Gerlach , Werner Köhler , Stig E. Holm , Barbara Wagner

{"title":"Biological and biochemical activities of a toxoid of erythrogenic toxin type A","authors":"Heide Knöll , Dieter Gerlach , Werner Köhler , Stig E. Holm , Barbara Wagner","doi":"10.1016/S0176-6724(88)80069-5","DOIUrl":"10.1016/S0176-6724(88)80069-5","url":null,"abstract":"<div><p>A toxoid of erythrogenic toxin type A (ET A) was prepared by formaldehyde treatment. Already 15 min after exposure to formaldehyde in isoelectric focusing the ET A band at pH 5.2 shifted to a band at pH 4.5.</p><p>In Ouchterlony double diffusion test ET A and its toxoid were found to be identical, in fused rocket immuno-electrophoresis a reaction of partial identity was seen. Formaldehyde treatment of ET A resulted in an apparent increase of electrophoretic mobility. In contrast to ET A, its toxoid is non-mitogenic, non-pyrogenic and has lost its ability to induce delayed type hypersensitivity. Binding of ET A toxoid to human peripheral lymphocytes is of the same magnitude as binding of gold-labelled ET A.</p></div><div><p>Durch Formaldehydbehandlung wurde aus hochgereinigtem erythrogenen Toxin Typ A (ET A) ein Toxoid hergestellt. Bereits nach 15 min Einwirkung des Formaldehyds trat in der isoelektrischen Fokussierung eine Verschiebung der scharfen ET A Bande bei pH 5,2 zu einer Bande bei pH 4,5 ein. Im Ouchterlony-Test erwiesen sich ET A und sein Toxoid als serologisch identisch, in der Fused-Rocket-Immunoelektrophorese war eine partielle Identität nachweisbar. Die Formaldehydbehandlung des ET A führte in der SDS-PAGE zu einer scheinbar stärkeren elektrophoretischen Mobilität. Im Gegensatz zu ET A ist das Toxoid nicht mitogen, apyrogen und hat seine Fähigkeit zur Induktion einer Hautreaktion vom verzögerten Typ verloren (10 000fache Gewichtsmenge Toxoid im Vergleich zur Minimalmenge des ET A von 5×l0<sup>−7</sup> mg/ml). ET A-Toxoid bindet sich in gleichem Ausmaß an humane periphere Lymphozyten wie goldmarkiertes ET A.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"269 4","pages":"Pages 468-478"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80069-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14277625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The effects of mycoplasma mobile 163 K on the ciliary epithelium of tracheal organ cultures 流动支原体163k对气管器官培养纤毛上皮的影响

Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology Pub Date : 1988-11-01 DOI: 10.1016/S0176-6724(88)80179-2

C. Stadtländer, H. Kirchhoff

{"title":"The effects of mycoplasma mobile 163 K on the ciliary epithelium of tracheal organ cultures","authors":"C. Stadtländer, H. Kirchhoff","doi":"10.1016/S0176-6724(88)80179-2","DOIUrl":"10.1016/S0176-6724(88)80179-2","url":null,"abstract":"<div><p><em>Mycoplasma (M.) mobile</em> 163 K, isolated from the gills of a tench (<em>Tinea tinea</em> L.), was examined for cytotoxic capacities using tracheal organ cultures from gnotobiotic rats and gnotobiotic piglets in pH-controlled experiments (pH 7.2–7.6).</p><p>The mycoplasmas caused an inhibition of the ciliary activity at incubation temperatures of 20, 25 and 30°C. The strongest cilia stopping effect was observed at an incubation temperature of 25°C, the optimal growth temperature of the mycoplasmas. No ciliostasis occurred at 37°C. The number of the organisms did clearly affect the severity of ciliostasis in the range from 4.0 × 10<sup>4</sup> to 7.7 × 10<sup>8</sup> c.f.u. in rat as well as in porcine tracheal organ cultures. Toxic substances, secreted by the mycoplasmas into the culture medium, could not be detected. In histological investigations cytopathological changes were observed in the epithelial cells, apparent in the destruction and loss of cilia, cytoplasmatic vacuolization, swelling of mitochondria, peripheral orientation of the nuclear chromatin and detachment of epithelial cells from each other and the basal membrane. The final stage of the infection was characterized by complete exfoliation of the epithelial cells and the complete destruction of the multi-layer epithelium. The localization of the mycoplasmas attached to the ciliary epithelium was shown by scanning and transmission electron microscopy.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"269 3","pages":"Pages 355-365"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80179-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14347372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Circulating Antigen of Toxoplasma gondii in Patients with AIDS: Significance of Detection and Structural Properties 艾滋病患者刚地弓形虫循环抗原的检测及其结构特性的意义

Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology Pub Date : 1988-11-01 DOI: 10.1016/S0176-6724(88)80167-6

Andreas Hassl , Horst Aspöck , Heinz Flamm

引用次数: 16

Evidence of Structural Proteins of Toxoplasma gondii in Sera of Experimentally Infected Mice 实验感染小鼠血清中刚地弓形虫结构蛋白的证据

Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology Pub Date : 1988-11-01 DOI: 10.1016/S0176-6724(88)80168-8

Andreas Hassl , Horst Aspöck , Heinz Flamm

引用次数: 4