WFUMB Ultrasound Open最新文献

{"title":"A wall tracking method to estimate ejection fraction from the parasternal long axis view in point of care ultrasound","authors":"Roberto Vega ,&nbsp;Arun Nagdev ,&nbsp;Masood Dehghan ,&nbsp;Seyed Ehsan Seyed Bolouri ,&nbsp;Brian Buchanan ,&nbsp;Jeevesh Kapur ,&nbsp;Jacob L. Jaremko ,&nbsp;Dornoosh Zonoobi","doi":"10.1016/j.wfumbo.2025.100097","DOIUrl":"10.1016/j.wfumbo.2025.100097","url":null,"abstract":"<div><h3>Objective:</h3><div>The left ventricular ejection fraction is a key metric for evaluating the systolic function in critically ill patients. Traditionally, it is computed using apical 2- and 4-chamber views in an echocardiogram; however, obtaining these views in an acutely ill patient in the emergency department is often difficult. A parasternal long-axis view, acquired with point of care ultrasound, is a faster and easier alternative. Unfortunately, the methods for estimating the ejection fraction from this view underperform when the left ventricular wall movement is not uniform or when its shape is not properly modeled as an ellipsoid. We propose a novel method that tracks the movement of the visible portions of the walls during the full cardiac cycle, and then estimates the ejection fraction based on that movement.</div></div><div><h3>Methods:</h3><div>We compared the performance of this method with the ejection fraction from the cardiology report on a dataset of 613 patients.</div></div><div><h3>Results:</h3><div>Our experiments showed an accuracy of 85% for identifying critically low values for ejection fraction (EF <span><math><mo>&lt;</mo></math></span> 30%) and 87% for abnormal ones (EF <span><math><mo>&lt;</mo></math></span> 50%). These values are comparable with the results obtained from the apical views and superior to current methods for the parasternal long-axis view.</div></div><div><h3>Conclusion:</h3><div>Since our method is fully automated, we expect that it can be adopted at scale in real-world clinical scenarios, giving practitioners a new tool to properly estimate the ejection fraction in clinically challenging scenarios.</div></div>","PeriodicalId":101281,"journal":{"name":"WFUMB Ultrasound Open","volume":"3 2","pages":"Article 100097"},"PeriodicalIF":0.0,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145324296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis of congenital heart diseases in children from 2D and 3D sonography using convolutional neural networks: A scoping literature review","authors":"Ayiga Majid ,&nbsp;Johnes Obungoloch ,&nbsp;Alfred Enywaku ,&nbsp;Obeti Francis ,&nbsp;Denis Jjuuko ,&nbsp;Eugene Bizimana ,&nbsp;Biryomumeisho Joshua ,&nbsp;Wasswa William","doi":"10.1016/j.wfumbo.2025.100096","DOIUrl":"10.1016/j.wfumbo.2025.100096","url":null,"abstract":"<div><div>Congenital heart diseases (CHDs) are the commonest congenital anomalies and a leading cause of neonatal morbidity and mortality worldwide. They affect approximately 1 % of live births globally. Prenatal detection with fetal echocardiography allows for timely referral and intervention. However, screening performance remains uneven because of operator dependence and resource limits. Convolutional neural networks (CNNs) offer automated image interpretation and have been widely applied to 2D and 3D fetal ultrasound (US) in recent years.</div><div>This scoping review maps CNN applications for prenatal CHD across 2010–September 2025. In adherence to the PRISMA-ScR methodology, we screened 845 records from PubMed, IEEE Xplore, Google Scholar, ScienceDirect and preprint servers and included 29 studies for synthesis. The volume of articles published on the topic rose sharply after 2020, with most datasets drawn from Asia and North America and no representation from sub-Saharan Africa. The most commonly reported architectures were DenseNet variants, U-Net families for segmentation, YOLO (You Only Look Once) variants for real-time detection, and ensemble hybrids for classification.</div><div>Reported internal performance is high in many studies, with top models achieving near-expert discrimination with AUCs (Area Under the Curve) up to 0.99. In our cohort, the mean AUC across studies reporting that metric was 0.911 (Standard Deviation-SD = 0.09; 95 % CI: 0.86–0.96; n = 11). Average sensitivity and specificity across studies reporting these metrics were 0.92 (SD = 0.04; n = 13) and 0.91 (SD = 0.04; n = 7), respectively. However, external and multi-centre validation remains limited, and performance commonly falls when models are tested on unseen centers or different scanner types. Methodological gaps include small or imbalanced lesion classes, inconsistent patient-level splitting, sparse reporting of deployment constraints, and limited fairness analyses.</div><div>Recent work shows promising directions such as multimodal fusion of B-mode and Doppler, lightweight networks, on-device optimization, federated and privacy-preserving training, and prospective protocols for multi-centre evaluation. To translate CNNs into clinical screening, we recommend coordinated efforts to build diverse, well-annotated repositories, adopt strict patient-level validation and external testing, report deployment metrics, and run prospective, pragmatic trials that measure clinical outcomes and health-economic impact. These steps can move CNNs from high-performance demonstrations to dependable tools that improve prenatal CHD detection equitably.</div></div>","PeriodicalId":101281,"journal":{"name":"WFUMB Ultrasound Open","volume":"3 2","pages":"Article 100096"},"PeriodicalIF":0.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145324295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uterine contrast-enhanced ultrasound (CEUS): new insights into adenomyosis using an innovative technique","authors":"Eva J.E. de Bock ,&nbsp;Barbara Stoelinga ,&nbsp;Jan Hein T.M. van Waesberghe ,&nbsp;Ferenc I. Kandi ,&nbsp;Catarina Dinis Fernandes ,&nbsp;Massimo Mischi ,&nbsp;Judith A.F. Huirne ,&nbsp;Nicole B. Burger ,&nbsp;Lynda J.M. Juffermans","doi":"10.1016/j.wfumbo.2025.100095","DOIUrl":"10.1016/j.wfumbo.2025.100095","url":null,"abstract":"<div><h3>Objectives</h3><div>Heavy menstrual bleeding, dysmenorrhea and subfertility are associated with adenomyosis, a benign uterine condition characterised by ectopic endometrium within the myometrium. Currently, diagnosing adenomyosis is challenging. Imaging the uterine microcirculation using contrast-enhanced ultrasound (CEUS) may improve diagnostics. We aimed to 1) determine the feasibility of transvaginal two-dimensional (2D) uterine CEUS, 2) compare features of adenomyosis on 2D CEUS with conventional ultrasound and Magnetic Resonance Imaging (MRI), and 3) qualitatively and quantitatively analyse 2D CEUS scans of patients with adenomyosis.</div></div><div><h3>Methods</h3><div>We included ten pre-menopausal adult patients with prominent direct Morphological Uterus Sonographic Assessment (MUSA) features of adenomyosis on B-mode. MRI scans were collected when clinically available. Conventional ultrasound and a 3-min transvaginal 2D CEUS scan were performed and analysed. Uterine wash-in and wash-out of the contrast agent were analysed qualitatively, and quantitatively using time-intensity curve parameters.</div></div><div><h3>Results</h3><div>Performing transvaginal CEUS scans was feasible, providing good visualisation of uterine macro- and microcirculation. CEUS visualised smaller and deeper-lying blood vessels than Doppler ultrasound. In the myometrium, non-to hypo-enhanced areas interspersed with hyper-enhanced regions, and vessels with enlarged lumen were observed. Hyper-echoic islands on B-mode showed either hypo- or hyper-enhancement on CEUS. Qualitative analysis and time-intensity maps showed clear heterogeneous myometrial perfusion in all patients with adenomyosis.</div></div><div><h3>Conclusions</h3><div>Transvaginal CEUS is a feasible technique for qualitative and quantitative analysis of the uterine macro- and microcirculation. It holds promise as a diagnostic imaging modality for adenomyosis.</div></div>","PeriodicalId":101281,"journal":{"name":"WFUMB Ultrasound Open","volume":"3 2","pages":"Article 100095"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145360999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating point-of-care ultrasound into internal medicine residency program in Ethiopia: An urgent call","authors":"Balew Arega","doi":"10.1016/j.wfumbo.2025.100094","DOIUrl":"10.1016/j.wfumbo.2025.100094","url":null,"abstract":"","PeriodicalId":101281,"journal":{"name":"WFUMB Ultrasound Open","volume":"3 2","pages":"Article 100094"},"PeriodicalIF":0.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra-operative and early post-operative cortical resistive indices in renal transplantation: Representative values according to graft function and time of scan","authors":"L. Thebridge ,&nbsp;C. Fisher ,&nbsp;V. Puttaswamy ,&nbsp;C. Pollock ,&nbsp;J. Clarke","doi":"10.1016/j.wfumbo.2025.100093","DOIUrl":"10.1016/j.wfumbo.2025.100093","url":null,"abstract":"<div><h3>Background</h3><div>Intra-operative ultrasound during renal transplantation identifies technical abnormalities allowing immediate surgical correction as required. Cortical resistive indices (RIs) assess graft perfusion and are now measured intra-operatively. The aim of this study was to evaluate RIs intra-operatively, and to 30 days post-operatively.</div></div><div><h3>Methods</h3><div>RIs in consecutive single renal grafts in adults were analysed from 246 grafts scanned intra-operatively, on day 1 post-operatively and subsequently according to physician preference, and 125 grafts scanned intra-operatively to day 30 post-operatively according to a formal protocol.</div></div><div><h3>Results</h3><div>There was delayed graft function (DGF) in 125 grafts. The mean (±sem) intra-operative RI of 0.599 (±0.009) was significantly lower (p &lt; 0.001) than post-operatively, with the highest RIs on days 3 and 7. RIs on day 30 were similar to day 1. RIs in grafts with function were significantly lower than grafts still requiring dialysis. Mean (±sem) cortical RIs on post-operative days 1, 3, 7 and 30 in functioning grafts were 0.662(±0.005), 0.712(±0.008), 0.703(±0.007) and 0.689 (±0.006) respectively, and in grafts still requiring dialysis 0.721(±0.010), 0.780(±0.010), 0.793(±0.011) and 0.751 (±0.034). The intra-operative RI predicted DGF (AUROC 0.774) as effectively as donor status (live/deceased) (AUROC 0.767). For deceased donor grafts, intra-operative RI was a more effective predictor (AUROC 0.710) than type of donor (DCD/DBD) (AUROC 0.610).</div></div><div><h3>Conclusions</h3><div>Intra-operative RIs are significantly lower than post-operatively and are indicative of post-operative graft function. Post-operative RIs in non-functioning grafts are consistently higher than in functioning grafts. Observed RIs differ according to the post-operative day of scan, which therefore should also be reported.</div></div>","PeriodicalId":101281,"journal":{"name":"WFUMB Ultrasound Open","volume":"3 2","pages":"Article 100093"},"PeriodicalIF":0.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating long-term skill retention following teledidactic vs. on-campus musculoskeletal ultrasound training: The TELMUS-follow-up study","authors":"Ricarda Neubauer ,&nbsp;Claus-Juergen Bauer ,&nbsp;Simon Michael Petzinna ,&nbsp;Pantelis Karakostas ,&nbsp;Elena Höhne ,&nbsp;Charlotte Behning ,&nbsp;Valentin Sebastian Schäfer ,&nbsp;Florian Recker","doi":"10.1016/j.wfumbo.2025.100092","DOIUrl":"10.1016/j.wfumbo.2025.100092","url":null,"abstract":"<div><h3>Purpose</h3><div>Musculoskeletal ultrasound (MSUS) relies on examiner competence, requiring ongoing practice, supervision, and quality assurance. Limited resources and densely packed medical curricula demand effective training methods to ensure long-term skill retention. Teledidatic as well as peer-assisted learning approaches have shown short-term results in training MSUS comparable with conventional in-class teaching. Consequently, this study aimed to evaluate the long-term retention of MSUS skills.</div></div><div><h3>Materials and methods</h3><div>One year after completing either the teledidactic or conventional course of a standardized MSUS elective (TELMUS study), medical students’ retention of MSUS skills in image acquisition and interpretation was assessed by a repeated objective structured clinical examination (OSCE). The follow-up OSCE scores of the teledidactic cohort and the on-campus cohort were compared to their previous results as well as between the two study groups.</div></div><div><h3>Results</h3><div>A total of 24 medical students of the initial 30 (80 %) participated in the follow-up study. In the follow-up OSCE, students enrolled in the teledidactic course achieved an average score of 52.88 (SD ± 6.12), which corresponds to 90.35 % of the results of the post-course assessment. Similarly, the on-campus participating students reached 91.99 % of their post-course OSCE with 52.25 (SD ± 7.45) points. No significant differences could be found between the two teaching cohorts (p &gt; 0.05).</div></div><div><h3>Conclusion</h3><div>Teledidactic teaching formats lead to an equivalent long-term maintenance of practical MSUS skills and serve as a sustainable didactic approach in medical education. The observed decline in skills across both cohorts highlights the critical need for repetition in student ultrasound education, ideally integrated into a longitudinal curriculum.</div></div>","PeriodicalId":101281,"journal":{"name":"WFUMB Ultrasound Open","volume":"3 2","pages":"Article 100092"},"PeriodicalIF":0.0,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144922745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonographic determination of bifurcation site of the tibial nerve at the tarsal tunnel","authors":"Donald Spears Wilcox ,&nbsp;Antonio Tom Howard ,&nbsp;Mark S. Mennemeier ,&nbsp;Jacqueline Rainey ,&nbsp;Charlotte Yates ,&nbsp;Melissa Allen ,&nbsp;Kevin D. Phelan","doi":"10.1016/j.wfumbo.2025.100090","DOIUrl":"10.1016/j.wfumbo.2025.100090","url":null,"abstract":"<div><h3>Objective</h3><div>Variability of Tibial nerve branching patterns with regard to the tarsal tunnel and malleolar-calcaneal axis has been established in a limited number of cadaveric studies with relatively few subjects. The current study used high-resolution ultrasonography to investigate tibial nerve branching variability, in-vivo, from a large number of healthy subjects.</div></div><div><h3>Methods</h3><div>High resolution ultrasonography was used to map the course of the tibial nerve, to determine the bifurcation point into to medial and lateral plantar nerves with regard to the malleolar-calcaneal axis and the tarsal tunnel, and to classify them into one of five established branching patterns. The in-vivo, ultrasonography findings were then compared with findings from cadaveric studies.</div></div><div><h3>Results</h3><div>The ultrasonography data for 156 feet (78 subjects-40 male, 38 female) of young, healthy individuals produced results similar to the cadaveric data with only small differences in each branching classification. Additionally, variation in the type of branching patterns between the left and right legs was observed in 23 % of the sample (i.e., 18 of 78 subjects).</div></div><div><h3>Conclusions</h3><div>In-vivo, high-resolution ultrasound appears to be a valid approach for investigating anatomic variation of superficial nerves as the US data converge with those for cadaveric dissection. Further, not only were the percentages of branching pattern types replicated from earlier cadaveric studies, but also differences in the types of branching patterns between the left and right legs of the same subjects were observed in 23 % of the sample. A side-to-side difference of this type has not been previously reported. Finally, a decided advantage of using ultrasound to map nerve branching, when possible, is that it is easier to obtain a larger sample than in typical cadaveric studies. The current study more than doubled the number of specimens collected in previous cadaveric studies which greatly improves the external validity of our findings.</div></div>","PeriodicalId":101281,"journal":{"name":"WFUMB Ultrasound Open","volume":"3 2","pages":"Article 100090"},"PeriodicalIF":0.0,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Twinkling artifact: a novelty finding for gel bleeding in silicone induced granuloma of breast implant capsule","authors":"Giulia Matheus e Castro ,&nbsp;Eduardo de Faria Castro Fleury","doi":"10.1016/j.wfumbo.2025.100091","DOIUrl":"10.1016/j.wfumbo.2025.100091","url":null,"abstract":"<div><div>The Twinkling artifact is an ultrasound finding commonly observed for the diagnosis of urolithiasis. The artifact consists of the formation of noise in the Doppler flowmetry study. However, when it comes to the evaluation of breast implants, there is no reported evidence in literature concerning its presence and significance.</div><div>In this case report, we describe a 41-year-old patient with implants for 8 years, asymptomatic, but who undergoes regular screening exams. In the Doppler ultrasound, the twinkling artifact was detected unilaterally inside an intracapsular mass compatible with silicone-induced granuloma of breast implant capsule (SIGBIC).</div><div>The objective of this case report is to demonstrate the strong correlation between the twinkling artifact and the presence of the silicone, foreign body. Specifically, when this artifact appears within the fibrous capsule, it strongly suggests gel bleeding. Therefore, the twinkling artifact can serve as an early sign of silicone bleeding in intact breast implants.</div></div>","PeriodicalId":101281,"journal":{"name":"WFUMB Ultrasound Open","volume":"3 2","pages":"Article 100091"},"PeriodicalIF":0.0,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144605154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of superficial high-frequency ultrasonography in assessing vulvovaginal lesions: A retrospective study","authors":"Kakoly Borthakur","doi":"10.1016/j.wfumbo.2025.100089","DOIUrl":"10.1016/j.wfumbo.2025.100089","url":null,"abstract":"<div><h3>Objective</h3><div>Ultrasound is a well-established imaging modality for assessing the female pelvis and perineum, primarily using convex or transvaginal probes. However, the vulvar region, being superficially located, remains inadequately evaluated by conventional ultrasound techniques. This study aims to explore the efficacy of superficial linear high-frequency ultrasonography, employing a targeted trans-perineal approach, in detecting and characterizing vulvovaginal lesions.</div></div><div><h3>Methods</h3><div>This retrospective study analyzed 61 patients presenting with clinical symptoms of pain or swelling in the vulvar region. Superficial high-resolution linear ultrasound was utilized as the primary diagnostic tool, with convex and intracavitatory probes employed in select cases. The ultrasound findings were correlated with clinical assessments, surgical outcomes, and pathological results.</div></div><div><h3>Results</h3><div>The ultrasound findings demonstrated a diagnostic accuracy of 95 % (CI - 89.5 %). Notably, 78.6 % of patients were treated based solely on ultrasound findings. While some cases underwent further evaluation with Magnetic Resonance Imaging (MRI), the additional findings did not significantly alter management or treatment outcomes. The predominant lesions identified were cystic in nature, originating from the vulva, vagina, or urethra, with most cases being acute inflammatory or infective lesions necessitating urgent medical intervention.</div></div><div><h3>Conclusion</h3><div>This study underscores the potential of ultrasound in the diagnosis and management of vulvar and superficial perineal lesions. The findings support the use of superficial high-frequency ultrasonography as a valuable tool in the morphological assessment of vulvovaginal lesions, facilitating timely diagnosis and treatment. This approach can potentially reduce healthcare costs and improve patient outcomes for those suffering from acute conditions, ultimately leading to improved clinical outcomes.</div></div>","PeriodicalId":101281,"journal":{"name":"WFUMB Ultrasound Open","volume":"3 2","pages":"Article 100089"},"PeriodicalIF":0.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144190362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of an anti-tumor immune response by sonodynamic therapy, combining 5-aminolevulinic acid and high-intensity focused ultrasound using the trigger pulse sonication","authors":"Tohru Satoh ,&nbsp;Rui Tada ,&nbsp;Taiki Yamaguchi ,&nbsp;Yoko Endo-Takahashi ,&nbsp;Takashi Kanno ,&nbsp;Mai Hayakawa ,&nbsp;Yoshiyuki Adachi ,&nbsp;Yoichi Negishi ,&nbsp;Jun Okamoto ,&nbsp;Shin Yoshizawa ,&nbsp;Ken Masamune","doi":"10.1016/j.wfumbo.2025.100088","DOIUrl":"10.1016/j.wfumbo.2025.100088","url":null,"abstract":"<div><div>Ultrasound-based cancer therapies offer non-invasive treatment options for deep-seated tumors, yet their clinical application is often limited by safety concerns. High-intensity focused ultrasound (HIFU) has demonstrated therapeutic potential, but its high-power requirements can damage surrounding healthy tissues, particularly near sensitive structures such as the gastrointestinal tract. The optimal balance between therapeutic efficacy and safety remains a significant challenge in ultrasound-based cancer treatment. Here we show that combining 5-aminolevulinic acid (5-ALA)-mediated sonodynamic therapy (SDT) with a novel HIFU system achieves effective tumor suppression at reduced acoustic intensities while enhancing anti-tumor immune responses. This novel system features the use of a trigger HIFU sequence that enables precise control over cavitation bubbles within the target tissue. In a murine colon carcinoma model, this approach demonstrated significant tumor growth inhibition with single-session treatment comparable to multiple HIFU sessions, while increasing tumor infiltration by activated CD8⁺ T cells and antigen-presenting dendritic cells. These findings extend beyond previous studies by revealing that SDT not only enables effective tumor treatment at lower, safer acoustic intensities but also promotes beneficial immune responses that could enhance therapeutic outcomes. This combination of reduced treatment intensity, shortened duration, and immune system activation suggests potential applications for treating tumors in anatomically sensitive locations. The demonstrated immunological effects also indicate possible synergies with existing cancer immunotherapies, potentially expanding treatment options for patients with deep-seated solid tumors.</div></div>","PeriodicalId":101281,"journal":{"name":"WFUMB Ultrasound Open","volume":"3 2","pages":"Article 100088"},"PeriodicalIF":0.0,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144190361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}