The Microbe最新文献

{"title":"β-galactosidase producing Lactiplantibacillus spp in intestinal microbiome mouse diarrhea model and metagenomic analyses","authors":"Mahanthesh Vasudha,&nbsp;Devaraja Gayathri","doi":"10.1016/j.microb.2024.100173","DOIUrl":"10.1016/j.microb.2024.100173","url":null,"abstract":"<div><div>Probiotics have attained significant interest in recent years as a result of their gut microbiome modulation and gastrointestinal health benefits. Numerous fermented foods contain LAB with <em>GRAS</em> grade and probiotic bacteria. The most promising isolates with high potentiality were selected for 16S rRNA gene sequence analysis and identified as <em>Lactiplantibacillus plantarum</em>, <em>L. fermentum</em>, <em>L. pentosus</em> and <em>Lactiplantibacillus</em> sp. The present study was conducted to determine the gastrointestinal symptoms after the consumption of milk and dairy products, lactose intolerance status using mice model. Post-weaning Balb/c mice were orally administered with probiotic bacteria to assess their efficiency in reducing lactose intolerance symptoms. They exhibited significantly reduced total feces weight and slower intestinal motility and they efficiently alleviated diarrhea symptoms within 6 h of lactose challenge in lactose intolerance-induced mice. Next generation sequence analysis was performed using intestinal content of model treated with <em>L. plantarum</em> GV54 and GV64, the intestinal bacterial diversity was studied using Illumina NGS analysis (16S rRNA hypervariable regions [V3 and V4]). NGS report confirmed the existence of the dominant phyla of the gut microbiome in mice are Bacteroidetes, Firmicutes and Proteobacteria. Due to the influence of the probiotic bacteria in the model group (GV54 and GV64), maximum abundance of phylum Firmicutes were observed when compared to the control group, whereas control group showed more abundance of phylum Bacteriodetes. However, the relative abundance of Bacteroidetes in the model group was decreased and Firmicutes abundance was increased. This indicates the genus abundance of <em>Lactobacillus</em> (<em>Lactiplantibacillus</em>) species or <em>Lactiplantibacillus plantarum</em> GV54 and GV64 bacteria. After treatment with probiotic bacteria <em>Lactobacillus</em> (<em>Lactiplantibacillus</em>) species, the probiotic bacterial abundance was increased consequently genera <em>Helicobacter, Clostridiales unclassified</em> and <em>Desulfovibrio</em> were decreased in the model groups. Probiotic bacteria has a positive impact on the regulation of intestinal microbial diversity in the tested mice system and it reflects that the intestinal microbiota of mice is dynamic and has positive impact on gut health. Considering this point, it is possible to promote <em>Lactiplantibacillus</em> spp. as probiotics and therapeutic food development at industrial scale.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"5 ","pages":"Article 100173"},"PeriodicalIF":0.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142422330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational systems and network biology perspective: Understanding Klebsiella pneumoniae infection mechanisms","authors":"Maulida Mazaya ,&nbsp;Novaria Sari Dewi Panjaitan ,&nbsp;Anis Kamilah Hayati","doi":"10.1016/j.microb.2024.100175","DOIUrl":"10.1016/j.microb.2024.100175","url":null,"abstract":"<div><div><em>Klebsiella pneumoniae</em> (<em>K. pneumoniae</em>) is a pathogen that has been identified as the leading cause of pneumonia and septicemia worldwide, compounded by its multi-drug resistant nature. Computational and bioinformatics approaches are yet understudied in terms of <em>K. pneumoniae</em>, and only recently systems and network biology-based approaches have gained attention for examining antimicrobial resistance. In this review, we highlight the prevalent use of computational systems and network biology methods in understanding <em>K. pneumoniae</em> infection mechanisms. We summarized ranges from basic methods including differential equations, network science analysis, and statistical insights into large processes, to intricate condition-specific genome-wide networks. More specifically, the availability of large-scale systematic genome-wide data, and detailed cellular and molecular information have enabled the use of mathematical modeling to study <em>K. pneumoniae</em> infection mechanisms. Thus, these approaches have proven to be effective in supporting academic exploration, complementing experimental studies, and deepening overall understanding in terms of <em>K. pneumoniae</em>. This review is essential to advance our knowledge of <em>K. pneumoniae</em> host-pathogen interactions and infection mechanisms. Furthermore, it serves as a valuable resource for researchers seeking guidance in selecting optimal computational systems and network biology models for <em>K. pneumoniae</em>-related investigations.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"5 ","pages":"Article 100175"},"PeriodicalIF":0.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142422332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of microglia in Zika virus pathogenesis: Possible diagnostic and therapeutic strategies","authors":"Subhrajyoti Paul,&nbsp;Sudipta Patra,&nbsp;Ayan Mondal,&nbsp;Gungun Sharma Adhikari,&nbsp;Piu Ghosh,&nbsp;Manojit Bysack,&nbsp;Rajen Dey","doi":"10.1016/j.microb.2024.100180","DOIUrl":"10.1016/j.microb.2024.100180","url":null,"abstract":"<div><div>The Zika virus, a member of the Flaviviridae family, is a mosquito-borne disease-causing virus that gained worldwide attention after a severe outbreak in Brazil in 2015. During this outbreak, some alarming evidence suggests that fetal microcephaly and other neuro-developmental disorders were associated with Zika infection. The virus also causes multi-organ failure and thrombocytopenia and also affects the eyes, leading to a potentially inflammatory disease called uveitis in adult individuals. It could be transmitted through sexual intercourse or the transplacental route, which is not commonly observed in other flaviviruses. In this review, we are interested in discovering the specific role of microglia in the pathogenesis of Zika virus-induced neuroinflammation. The virus comes into contact with microglial cells through the blood and causes activation and neuroinflammatory consequences, leading to the death of developing neurons and ultimately resulting in altered neuron-glia interaction. Despite its severity, there is still no specific medicine or vaccine available to combat the Zika infection. An essential aspect of this review is to identify cost-effective treatment strategies and accurate diagnostic methods for broader populations. In conclusion, an understanding of the cellular basis of Zika pathogenesis can help ensure potential treatment strategies to combat the global outbreak of the Zika virus.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"5 ","pages":"Article 100180"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142422334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling of cellulolytic fungal consortium from humus soil for efficient lignocellulosic waste degradation","authors":"H.K. Narendra Kumar ,&nbsp;N. Chandra Mohana ,&nbsp;Jayarama Reddy ,&nbsp;M.R. Abhilash ,&nbsp;S. Satish","doi":"10.1016/j.microb.2024.100183","DOIUrl":"10.1016/j.microb.2024.100183","url":null,"abstract":"<div><div>Cellulose is a complex polysaccharide composed of β 1, 4 glycosidic linkages and these linkages are broken down by a complex enzyme system called cellulase. Cellulase is an enzyme complex associated with β 1, 4 endoglucanase, β 1, 4 exoglucanase, and β glucosidase. The fungal strains were isolated from decomposed humus soil and screened for the best cellulolytic activity. Thirty-two isolates were screened among these; three were selected based on their morphological characters and hydrolytic capacity, viz, <em>Cladosporium oxysporum</em> N5, <em>Aspergillus sigurros</em> N6, and <em>Cladosporium cladosporioides</em> N12. Among these fungi, <em>Cladosporium oxysporum</em> N5 shows potential hydrolytic activity and was selected for optimization studies and evaluation of their cellulolytic capability. Cellulolytic strain <em>Cladosporium oxysporum</em> N5 produced maximum amount of cellulase enzyme at 5 days of incubation at 30 ℃ and pH 7, viz, β 1, 4 endoglucanase (167.83± U/ml<strong>)</strong>, β 1, 4 exoglucanase (29.04± U/ml<strong>)</strong>, and β glucosidase (34.07± U/ml). SEM coupled Energy dispersive X-ray and FTIR spectroscopy methods revealed the cellulose degradation efficiency of fungal cellulase by microscopic, elemental percentage, and functional group, respectively. An affordable and eco-friendly fungal-based cellulase that will boost industrially important commodity production such as food, beverages, pharmaceuticals, fertilisers, and biofuels.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"5 ","pages":"Article 100183"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142422174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Klebsiella rhizobacterium deregulates the metabolism of phytopathogenic Aspergillus flavus during in-vitro assays and confers protective functions","authors":"Shree P. Pandey ,&nbsp;Shivam Singh ,&nbsp;Deepesh Khandwal ,&nbsp;Avinash Mishra ,&nbsp;Bhagya Shree Acharya ,&nbsp;Suman Bakshi ,&nbsp;Sundeep Kumar ,&nbsp;Vinod Mishra ,&nbsp;Sandeep Sharma","doi":"10.1016/j.microb.2024.100181","DOIUrl":"10.1016/j.microb.2024.100181","url":null,"abstract":"<div><div>In previous investigations, we have identified a rhizobacterium (<em>Klebsiella</em> sp. MBE02) that confers host protection against several phytopathogenic fungi. For instance, this rhizobacterium prevents <em>Aspergillus flavus</em> infection and promotes peanut growth and fitness in controlled and field-conditions. The mechanistic basis of the protective function offered by this rhizobacterium is not completely understood. MBE02 directly restricts the growth of the pathogenic fungi, which led us to hypothesize that it may strongly dysregulate the metabolism of <em>A. flavus</em>, and inhibit critical metabolic processes of the fungus, which severely restricts pathogen growth. We have tested this hypothesis by using untargeted metabolite profiling. Sixty-nine <em>A. flavus</em> metabolites accumulated differentially due to the presence of the MBE02. MBE02 could inhibit several important metabolic pathways, which include the biosynthesis of critical primary metabolites such as amino acids and fatty acids. It also impacts energy metabolism of the fungus, and that the accumulation of several structural components, including of the cell wall, were strongly inhibited. MBE02 abrogated the accumulation of disease-causing metabolites in <em>A. flavus</em>, whereas the accumulation of metabolites that inhibit fungal growth were enhanced. On the other hand, <em>A. flavus</em> did not strikingly impact the accumulation of metabolites of the MBE02. Our investigation supports the hypothesis that <em>Klebsiella</em> sp. MBE02 mediates protective function by directly impairing the pathogen’s metabolism.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"5 ","pages":"Article 100181"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the effect of human intestinal microbiota bacteria on the bioremediation of commonly used pesticides by liquid chromatography","authors":"Ahmet Aktaş ,&nbsp;İlker Aras Göçer ,&nbsp;Yaşar Naki̇poğlu","doi":"10.1016/j.microb.2024.100170","DOIUrl":"10.1016/j.microb.2024.100170","url":null,"abstract":"<div><div>Pesticides are defined as chemical substances used to protect a variety of plants from parasite infestation. Due to their chemical structure, they have a long half-life and the capacity to form residues, and therefore many fruits can contain pesticide residues. Pesticide exposure may occur via inhalation, ingestion, or direct contact. The European Commission's directive restricts the use of pesticides and sets a residue limit of 0.1 μg/L. The aim of this study is to determine the impact of bacteria in the human gut microbiota on the biodegradation of commonly used pesticides. This study investigated the effects of five bacterial strains, namely <em>Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae (K. pneumoniae)</em>, and <em>Enterococcus faecalis (E. faecalis</em>), isolated from neonatal rectal swab samples and, blood samples on the degradation of pesticides using liquid chromatography and mass spectrometry. (LC-MS). All bacteria resulted in significant pesticide degradation (<em>p</em> &lt; 0.05). <em>E. faecalis</em> caused significantly more degradation than <em>K. pneumoniae</em> (<em>p</em> = 0.043). No significant difference was observed between the degradation rates of pesticides and other bacteria (<em>p</em> &gt; 0.05). The results demonstrate that bacteria can be used to clean water and soil contaminated with pesticides. Therefore, we do not have information about possible mutations in DNA. This is the biggest limitation of this study. Therefore, our study data needs to be supported with study data including more strains and examining bacterial DNA with new generation sequencing methods.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"5 ","pages":"Article 100170"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic susceptibility pattern of carbapenem resistant Escherichia coli and Klebsiella pneumoniae, 2019","authors":"Ibrahim Mohammed Hussaini ,&nbsp;Ahmed Babagida Suleiman ,&nbsp;Olayeni Stephen Olonitola ,&nbsp;Rukayat Avosuahi Oyi","doi":"10.1016/j.microb.2024.100174","DOIUrl":"10.1016/j.microb.2024.100174","url":null,"abstract":"<div><div>The rise in the prevalence of carbapenem resistant bacteria worldwide has made the treatment of nosocomial infections challenging. This is because carbapenem resistant bacteria harbor multiple resistance genes aside the carbapenemase genes. This study was conducted to determine the susceptibility pattern of carbapenem resistant isolates of <em>Escherichia coli</em> and <em>Klebsiella pneumoniae.</em> Previously isolated and characterized carbapenem resistant <em>E. coli</em> and <em>K. pneumoniae</em> were screened for their susceptibility to ceftriaxone, trimethoprim-sulphamethoxazole, doxycycline, amikacin, gentamicin, nalidixic acid, chloramphenicol, colistin, tigecycline and fosfomycin by the Kirby Bauer technique. EUCAST and CLSI breakpoints were used to interpret the zones of inhibition. High level of resistance was observed among the isolates to ceftriaxone (100.00 %), trimethoprim-sulphamethoxazole (100.00 %) and doxycycline (83.33 %). Two-third of the isolates (66.67 %) were observed to be susceptible to amikacin, while 33.33 % of the isolates were gentamicin and nalidixic acid susceptible. Susceptibility to chloramphenicol and colistin was recorded in 16.67 % of the isolates while all the screened isolates (100.0 %) were susceptible to fosfomycin and tigecycline. A higher antibiotic resistance rate was observed among isolates habouring carbapenemase genes (<em>bla</em>_NDM, <em>bla</em>_OXA or both). The MAR indices of the carbapenem resistant isolates ranged between 0.46 and 0.82. So also, all the isolates screened were observed to be resistant to multiple antibiotics, hence MDR isolates. In conclusion, the carbapenem resistant isolates were resistant to multiple antibiotics but were however susceptible to tigecycline, fosfomycin and amikacin.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"5 ","pages":"Article 100174"},"PeriodicalIF":0.0,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced antimicrobial activity of naringin-ciprofloxacin combination against Pseudomonas aeruginosa PAO1: Unveiling quorum-sensing mediated molecular mechanisms in biofilm formation and virulence","authors":"Pia Dey ,&nbsp;Rakesh De ,&nbsp;Debaprasad Parai ,&nbsp;Sk Tofajjen Hossain ,&nbsp;Samir Kumar Mukherjee","doi":"10.1016/j.microb.2024.100171","DOIUrl":"10.1016/j.microb.2024.100171","url":null,"abstract":"<div><div>Natural products offer a wealth of potential antimicrobial agents that target various cellular signalling pathways of bacterial pathogens linked to public health issues, especially for managing biofilm-associated infections caused by <em>Pseudomonas aeruginosa</em>. Naringin, a flavanone glycoside, alone or in combination with ciprofloxacin was reported to inhibit biofilm development effectively in <em>P. aeruginosa</em>. However, the molecular mechanisms behind the antibiofilm activities of naringin are yet to be fully understood. This study was performed to unveil the mechanistic role of naringin-ciprofloxacin (NC) combinations for its antibiofilm and antivirulence attributes using <em>P. aeruginosa</em> PAO1 as the model organism. NC combinations interfered with the production of different virulence factors in <em>P. aeruginosa</em>. The RT-qPCR analysis suggested that NC combinations notably decreased the expression of <em>lasI</em> and <em>rhlI</em>, the autoinducer synthase genes, and the expression of <em>lasR</em> and <em>rhlR</em> as well. Additionally, molecular docking analysis showed naringin could disrupt the interactions between LasI-LasR, and RhlI-RhlR, thus potentially could affect QS-network of <em>P. aeruginosa</em>. The change in the expression and interaction pattern of the QS regulator genes by the NC combinations may account for the reduction in biofilm formation and pathogenic behaviour in <em>P. aeruginosa</em>. Thus, the combination of naringin and ciprofloxacin might offer a promising alternative to restrict the biofilm development by <em>P. aeruginosa</em>, and could serve as a reference for addressing antibiotic resistance for managing biofilm-assisted pseudomonad infections.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"5 ","pages":"Article 100171"},"PeriodicalIF":0.0,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142319480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the arsenal against antibiotic resistance: Antibacterial peptides as broad-spectrum weapons targeting multidrug-resistant bacteria","authors":"Ibrahim Mohammed Hussaini ,&nbsp;Asmau Nna Sulaiman ,&nbsp;Salim Charanchi Abubakar ,&nbsp;Tariq Mohammed Abdulazeez ,&nbsp;Murjanatu Muhammad Abdullahi ,&nbsp;Mamunu Abdulkadir Sulaiman ,&nbsp;Abubakar Madika ,&nbsp;Musa Bishir ,&nbsp;Aliyu Muhammad","doi":"10.1016/j.microb.2024.100169","DOIUrl":"10.1016/j.microb.2024.100169","url":null,"abstract":"<div><div>The emergence of antibiotic resistant bacteria is a serious challenge of public health concern. The increasing concern of antibiotic resistance has necessitated the need for alternative therapeutic options. Antibacterial peptides are broad-spectrum bactericidal compounds with promising activity against multidrug resistant bacteria, hence their recognition as an emerging therapeutic approach against multidrug resistant bacteria. Naturally occurring antibacterial peptides are mostly sourced from plants, amphibians, mammals, insects, and microorganisms. Aside from naturally occurring antibacterial peptides, many synthetic peptides have also been produced. Most peptides exhibit antibacterial activity by physically disrupting the cell membranes of bacteria, while other peptides target non-membrane structures such as bacterial cell walls and intracellular components of the bacteria. This review highlights the antibacterial activity of antibacterial peptides against bacteria including drug-resistant bacterial strains. Some of these peptides exhibited antibacterial activity when used alone while others exhibited synergistic antibacterial activity when used with antibiotics. The review also outlines the mechanism of antibacterial peptide resistance among bacteria and various techniques used for the chemical modification of peptides to enhance their bioactivity and stability. Existing antimicrobial peptide databases were also summarized in this review. This review gives an insight into the therapeutic use of antibacterial peptides as an alternative therapeutic option for the treatment of infection caused by antibiotic resistant bacterial strains.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"5 ","pages":"Article 100169"},"PeriodicalIF":0.0,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multidrug-resistant Klebsiella pneumoniae and Klebsiella variicola isolated from patients in Portuguese hospitals: Genomic and phenotypic characterization","authors":"Joana Castro ,&nbsp;Daniela Araújo ,&nbsp;Hugo Oliveira ,&nbsp;Liliana Fernandes ,&nbsp;Ricardo Oliveira ,&nbsp;Erick Brinks ,&nbsp;Gyu-Sung Cho ,&nbsp;Charles Franz ,&nbsp;Maria José Saavedra ,&nbsp;Sónia Silva ,&nbsp;Carina Almeida","doi":"10.1016/j.microb.2024.100172","DOIUrl":"10.1016/j.microb.2024.100172","url":null,"abstract":"<div><div>The emergence of antibiotic-resistant <em>Klebsiella</em> spp. has become a huge problem in clinical settings. In this study, we aimed to characterize the molecular and phenotypic features of 21 MDR <em>Klebsiella</em> isolates (<em>n</em> = 19 of <em>K. pneumoniae</em>, and <em>n</em> = 2 of <em>K. variicola</em>) isolated from patients admitted to two central hospitals in northern Portugal by using whole-genome sequencing (WGS), followed by an <em>in vitro</em> assessment of biofilm formation and <em>in vivo</em> evaluation of the pathogenicity in a <em>Galleria mellonella</em> larval model. Our findings showed a high prevalence of O1/O2 serotypes (14/21; 67 %) among the isolates tested, which is consistent with previous reports from Portugal. In contrast, a wide variety of K locus serotypes was found, where ST15-KL19 (4/21; 19 %) associated to serotype O1/O2v2 was the dominant one. Within the O1/O2v2 serotype, a ST10-KL151 <em>Klebsiella variicola</em> (strain H97) harboured a high number of virulence genes. We also found statistical differences in the ability to produce biofilm biomass within the strains, with a ST280-KL23 <em>K. pneumoniae</em> outcompeting nine other strains. According to our results, the most prominent serotype able to cause the death of <em>G. mellonella</em> was the KL105-O1/O2v2. Our findings emphasized the importance of the conducting continuous molecular surveillance in order to reveal the key molecular features to be considered in the development of novel strategies to treat <em>Klebsiella</em> spp.-associated infections.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"5 ","pages":"Article 100172"},"PeriodicalIF":0.0,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2950194624001390/pdfft?md5=2f48816e4a3abc26cf35dd6e980cdd4a&pid=1-s2.0-S2950194624001390-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}