The Journal of Trace Elements in Experimental Medicine最新文献_第6页

Enhanced tolerance to high cytostatic doses by means of oligoelements Mn, Se, and Zn plus Lachesis muta venom: In vivo and in vitro studies 通过微量元素Mn、Se和Zn加上变异Lachesis毒液增强对高细胞抑制剂量的耐受性：体内和体外研究

The Journal of Trace Elements in Experimental Medicine Pub Date : 2003-01-01 DOI: 10.1002/jtra.10024

Ernesto J. Crescenti, Máximo Croci, Elena Rivera, Rosa M. Bergoc

{"title":"Enhanced tolerance to high cytostatic doses by means of oligoelements Mn, Se, and Zn plus Lachesis muta venom: In vivo and in vitro studies","authors":"Ernesto J. Crescenti, Máximo Croci, Elena Rivera, Rosa M. Bergoc","doi":"10.1002/jtra.10024","DOIUrl":"https://doi.org/10.1002/jtra.10024","url":null,"abstract":"We evaluated, in vivo and in vitro, the increased tolerance to treatment with high doses of chemotherapeutic drugs produced by the administration of oligoelements (Zn, Se, Mn) plus Lachesis muta venom (O-LM). Cyclophosphamide, methotrexate, and 5-fluoruracil (CMF) were administrated to male rats treated daily with O-LM. The doses of CMF tested covered from 1- to 20-fold the dose used in human patients. Human neoplastic and normal cell lines were challenged in vitro with increasing doses of 5-fluoruracil (5-FU) in the presence and absence of O-LM. Results indicated a significantly higher global survival in O-LM-treated rats versus Control rats. LD50 in O-LM rats was 18.5-fold the basic CMF dose versus 14.1 in controls. Eighty percent of Control rats showed severe bone marrow aplasia, sepsis, and secondary leukemia, while only 20% of O-LM-treated rats showed these effects and no leukemia cases were observed. O-LM treatment produced a significant inhibition of cell proliferation of malignant cells, while in normal cells O-LM increased colony formation. In transformed cells, the cytostatic effect of 5-FU was significantly enhanced by O-LM pre-treatment, in contrast with the protective effect exerted on normal cells. Toxicological studies clearly demonstrated that O-LM did not produce any toxic or undesirable effect when administered to normal animals. Whole body weight, water and food consumption, and laboratory parameters remained unchanged after O-LM administration, even with a dose 10-fold higher than that employed in the experimental work. Results obtained afford the possibility of applying this protective treatment to human patients undergoing high-dose chemotherapy. J. Trace Elem. Exp. Med. 16:39–53, 2003. © 2003 Wiley-Liss, Inc.","PeriodicalId":101243,"journal":{"name":"The Journal of Trace Elements in Experimental Medicine","volume":"16 1","pages":"39-53"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jtra.10024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72324474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Recent advances in the biochemistry of chromium(III) 铬（III）的生物化学研究进展

The Journal of Trace Elements in Experimental Medicine Pub Date : 2003-01-01 DOI: 10.1002/jtra.10038

John B. Vincent

{"title":"Recent advances in the biochemistry of chromium(III)","authors":"John B. Vincent","doi":"10.1002/jtra.10038","DOIUrl":"https://doi.org/10.1002/jtra.10038","url":null,"abstract":"The biochemistry of chromium(III) [Cr(III)] has been a poorly understood field of endeavor; studies of the biochemistry of none of the other transition metals have been as problematic. Despite four decades of investigation, only recently has a clear picture of the role of Cr potentially been defined. The biologically important form is the trivalent ion. Cr(III) apparently is required for proper carbohydrate and lipid metabolism in mammals, although Cr deficiency is difficult to achieve. Conditions that increase circulating glucose and insulin concentrations increase urinary chromium output. Chromium is excreted after an insulin challenge, probably in the form of the oligopeptide chromodulin. Chromodulin may be the key to understanding the role of chromium at a molecular level as the molecule has been found to bind to activated insulin receptor, stimulating its kinase activity. A mechanism for the action of chromodulin has recently been proposed; this mechanism can serve as a potential framework for further studies to test the role of chromium in metabolism. An examination of the use of chromium picolinate, [Cr(pic)3], illustrates the difficulties associated with biochemical studies dealing with Cr(III). J. Trace Elem. Exp. Med. 16:227–236, 2003. © 2003 Wiley-Liss, Inc.","PeriodicalId":101243,"journal":{"name":"The Journal of Trace Elements in Experimental Medicine","volume":"16 4","pages":"227-236"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jtra.10038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72362794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Zinc and copper in breast-milk and home-prepared milk fed to urban infants from low-income families 低收入家庭城市婴儿母乳和自制母乳中的锌和铜

The Journal of Trace Elements in Experimental Medicine Pub Date : 2002-01-01 DOI: 10.1002/jtra.10010

José G. Dórea

{"title":"Zinc and copper in breast-milk and home-prepared milk fed to urban infants from low-income families","authors":"José G. Dórea","doi":"10.1002/jtra.10010","DOIUrl":"https://doi.org/10.1002/jtra.10010","url":null,"abstract":"When prepared in the home, differences in mineral concentrations between human and cow's milk can be further increased due to water supply and utensils. Comparisons were made between the concentrations of zinc and copper and their caloric densities of breast-milk and home-prepared milk. Samples of breast-milk and home-prepared milk representative of the milk feedings of the first 6 months were analyzed for zinc and copper by atomic absorption spectrophotometry. The initial high levels at the first day (8 μg/mL) decrease 47% during the first week, and are decreased by 59% at day 15. By the first, second, and third months, the respective decreases are 67%, 84%, and 85%. At 6 months, the breast-milk zinc concentration is only 12% (0.93 μg/mL) of its initial value. In the analyzed samples of breast-milk, the patterns of changes in zinc and copper concentrations were comparable to values in literature. However, in home-prepared milk consumed by infants who are 1–6 months old, no systematic pattern of change was observed. Relatively higher concentrations of zinc and copper in home-prepared milks resulted in metallocalorie ratios comparable to breast milk. The zinc to copper ratio varied relatively less in human milk. Reconstitution of powdered milk products, which comprise the main food item of babies during early weaning, may result in trace-element nutrition unbalance. J. Trace Elem. Exp. Med. 15:123–129, 2002. © 2002 Wiley-Liss, Inc.","PeriodicalId":101243,"journal":{"name":"The Journal of Trace Elements in Experimental Medicine","volume":"15 3","pages":"123-129"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jtra.10010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72314034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Chemical forms of metallothionein and cellular oxidative stress in human hepatocellular carcinoma: A comparative study with the hepatitis of LEC rat 人肝细胞癌中金属硫蛋白的化学形式和细胞氧化应激：与LEC大鼠肝炎的比较研究

The Journal of Trace Elements in Experimental Medicine Pub Date : 2002-01-01 DOI: 10.1002/jtra.10003

Akihiro Nakayama, Riichi Tawa, Nobuyuki Masuyama, Masaaki Ebara, Hiroyuki Fukuda, Keiji Suzuki, Katsuyuki Nakajima, Hiromu Sakurai

{"title":"Chemical forms of metallothionein and cellular oxidative stress in human hepatocellular carcinoma: A comparative study with the hepatitis of LEC rat","authors":"Akihiro Nakayama, Riichi Tawa, Nobuyuki Masuyama, Masaaki Ebara, Hiroyuki Fukuda, Keiji Suzuki, Katsuyuki Nakajima, Hiromu Sakurai","doi":"10.1002/jtra.10003","DOIUrl":"https://doi.org/10.1002/jtra.10003","url":null,"abstract":"Metallothionein (MT) has been described as a cell-protective protein that binds several heavy metals. In mammals, MT plays an important physiological role that includes detoxifying heavy metals, accumulating essential trace elements in cells to maintain homeostasis, and scavenging reactive oxygen species or free radicals. In contrast, the generation of hydroxyl radical (·OH) has been reported in the reaction of hydrogen peroxide and copper (Cu)-MT separated from the livers of Long–Evans Cinnamon (LEC) rats, an animal model of human hepatic injury with unusual accumulation of Cu-MT in the liver. On the other hand, Cu has been reported to accumulate in human hepatocellular carcinoma (HCC). Thus, we investigated whether the induction of MT, accumulation of Cu, and enhancement of oxidative stress are relevant to the development of human HCC, similarly to the observations in the liver of LEC rats. Although the kinetic behaviors of metal and MT levels were different in the livers of humans and LEC rats, Cu-MT induction in the tumor portion of human HCC tissues was suggested to occur similarly to that in the liver of LEC rats. In addition, it was found that oxidative stress in terms of the level of thiobarbituric acid-reactive substances was enhanced in the tumor portion of human HCC, corresponding well with increasing in the age of LEC rats, which developed jaundice and hepatitis. On the basis of these results, we conclude that the induction of Cu-MT in human HCC might be related to the hepatic oxidative stress, which in turn develops hepatic injury involving carcinoma. J. Trace Elem. Exp. Med. 15:31–45, 2002. ©2002 Wiley-Liss, Inc.","PeriodicalId":101243,"journal":{"name":"The Journal of Trace Elements in Experimental Medicine","volume":"15 1","pages":"31-45"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jtra.10003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72320649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Effect of melatonin treatment on serum and tissue zinc levels in rats 褪黑素对大鼠血清和组织锌水平的影响

The Journal of Trace Elements in Experimental Medicine Pub Date : 2002-01-01 DOI: 10.1002/jtra.1053

Güler Öztürk, Şule Coşkun, Deniz Erbaş, Bülent Altunkaynak

{"title":"Effect of melatonin treatment on serum and tissue zinc levels in rats","authors":"Güler Öztürk, Şule Coşkun, Deniz Erbaş, Bülent Altunkaynak","doi":"10.1002/jtra.1053","DOIUrl":"https://doi.org/10.1002/jtra.1053","url":null,"abstract":"Melatonin (MEL) is the main neuro-hormone of the pineal gland. The main physiological function of MEL is to modulate variations of the light-dark cycle and seasonal periodicity on various physiological functions. Zinc (Zn) is required as a catalytic component for more than 200 enzymes and is a structural constituent of many proteins, hormones, neuropeptides, and receptors. In addition, tissue Zn levels have been found to be altered after pinealectomy in rats. Recent findings have shown that MEL can modulate Zn turnover. In the present study, we investigated the effect of MEL treatment on the tissue and serum Zn levels. Male Spraque–Dawley rats (n = 24) were used. Rats received seven daily subcutaneous injections of MEL (10 mg/kg). After 7 days, rats were decapitated at 10:00 a.m. and 02:00 a.m., which were the periods of lowest and highest serum MEL levels, respectively. Blood and tissue samples were collected, and Zn levels were measured by atomic absorption spectrophotometry. In conclusion, MEL increased Zn levels in the salivary glands and small intestine. Daytime liver Zn levels and nighttime serum Zn levels increased in MEL-treated group. There was no significant difference in brain after MEL treatment. Nighttime serum MEL levels were higher than daytime in both control and MEL-treated group. In addition, MEL levels in MEL-treated groups were significantly higher than those of control groups. It is clear that there is a tight relationship between Zn and MEL. J. Trace Elem. Exp. Med. 15:1–8, 2002. © 2002 Wiley-Liss, Inc.","PeriodicalId":101243,"journal":{"name":"The Journal of Trace Elements in Experimental Medicine","volume":"15 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jtra.1053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72362084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Dietary copper deficiency increases inducible nitric oxide synthase-mediated vascular dilation in rat aorta† 膳食铜缺乏增加诱导型一氧化氮合酶介导的大鼠主动脉血管扩张†

The Journal of Trace Elements in Experimental Medicine Pub Date : 2002-01-01 DOI: 10.1002/jtra.10005

Leila Gobejishvili, Jack T. Saari, Ayotunde S.O. Adeagbo, Xiaodong Zhang, Dale A. Schuschke

{"title":"Dietary copper deficiency increases inducible nitric oxide synthase-mediated vascular dilation in rat aorta†","authors":"Leila Gobejishvili, Jack T. Saari, Ayotunde S.O. Adeagbo, Xiaodong Zhang, Dale A. Schuschke","doi":"10.1002/jtra.10005","DOIUrl":"https://doi.org/10.1002/jtra.10005","url":null,"abstract":"The attenuation of endothelium-dependent nitric oxide (NO)-mediated vasodilation is a consistent finding in both conduit and resistance vessels during copper deficiency. However, there is often no effect on systemic blood pressure in experimental animals, suggesting that peripheral vascular resistance is not altered. We hypothesized that baseline vascular smooth muscle relaxation may be maintained by a chronic increase in inducible NO synthase (iNOS) expression, as has been documented in hearts of copper-deficient rats. We used endothelium-denuded rat aortic rings to examine the role of iNOS in the regulation of vascular reactivity during dietary copper deficiency. Male weanling rats were fed a copper-adequate (CuA, 5.6 mg Cu/kg diet) or copper-deficient diet (CuD, 0.33 mg Cu/kg diet) for 4 weeks. The induction of “functional” iNOS was indicated by a relaxation response to the NO precursor L-arginine or to Cu,Zn-superoxide dismutase (SOD), which preserves basal NO. Time to 50% relaxation in response to either compound was significantly shorter in the CuD than in the CuA aortas. The maximal relaxation response to L-arginine was blocked by the iNOS inhibitor L-NIL, and the relaxation response to Cu,Zn-SOD was blocked by the NO-sensitive guanylate cyclase inhibitor ODQ. Maximal activation of iNOS expression with lipopolysaccharide pretreatment did not cause a difference in vascular relaxation between dietary groups in response to L-arginine. Expression of the iNOS protein in the aortas was also not different between groups. These results suggest that although there is no apparent increase in protein expression, copper deficiency increases baseline iNOS activity in the vascular wall. J. Trace Elem. Exp. Med. 15:85–95, 2002. © 2002 Wiley-Liss, Inc.","PeriodicalId":101243,"journal":{"name":"The Journal of Trace Elements in Experimental Medicine","volume":"15 2","pages":"85-95"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jtra.10005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72331910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Further studies on the interrelationship of aluminum and silicon in patients receiving aluminum hydroxide therapy for dyspepsia and factors that relate to the solubilization of aluminum 接受氢氧化铝治疗消化不良患者铝和硅相互关系的进一步研究以及与铝溶解有关的因素

The Journal of Trace Elements in Experimental Medicine Pub Date : 2002-01-01 DOI: 10.1002/jtra.1054

N.B. Roberts, H. Zhu, J.Y. Kim, H.R. Shin, J.I. Kim, S.Y. Choi

{"title":"Further studies on the interrelationship of aluminum and silicon in patients receiving aluminum hydroxide therapy for dyspepsia and factors that relate to the solubilization of aluminum","authors":"N.B. Roberts, H. Zhu, J.Y. Kim, H.R. Shin, J.I. Kim, S.Y. Choi","doi":"10.1002/jtra.1054","DOIUrl":"https://doi.org/10.1002/jtra.1054","url":null,"abstract":"It is known that the intake of aluminum-containing antacids is related to increases in serum concentration and the urinary output of aluminum, and that silicon intake as orthosilicic acid may facilitate renal excretion of aluminum. However, how either the amount of antacid ingested or the therapy period affects these and the subsequent interrelationship of aluminum and silicon has not been fully investigated. In addition, factors that affect the solubility of aluminum hydroxide need further clarification. A study of 122 patients with dyspepsia who were receiving aluminum hydroxide therapy and 144 healthy controls confirmed that, in the patients, serum and urine aluminum (0.39 ± 0.21 and 2.02 ± 2.0 μmol/L, mean ± 1 SD, respectively) were slightly but significantly increased (P 3.5 the solubility of aluminum reduced significantly. However, addition of 5 or 20 mmol/L phosphate had no significant effect. With a synthetic food, solubility of aluminum was markedly increased even at the elevated pH 5.5. Thus, the pH, as well as the chemical complexity of the mixture, is an important factor in affecting solubilization of aluminum. The potential of increased exposure to aluminum could be dependent on co-ingestion with food components and may explain the increased risk to renal failure patients on aluminum-containing phosphate binders. J. Trace Elem. Exp. Med. 15:9–19, 2002. © 2002 Wiley-Liss, Inc.","PeriodicalId":101243,"journal":{"name":"The Journal of Trace Elements in Experimental Medicine","volume":"15 1","pages":"9-19"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jtra.1054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72362083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Neurotoxic effects of differential doses of lead on rat brain followed by recovery 不同剂量铅对大鼠脑的神经毒性影响及恢复

The Journal of Trace Elements in Experimental Medicine Pub Date : 2002-01-01 DOI: 10.1002/jtra.10009

Bimla Nehru, Pardeep Sidhu

{"title":"Neurotoxic effects of differential doses of lead on rat brain followed by recovery","authors":"Bimla Nehru, Pardeep Sidhu","doi":"10.1002/jtra.10009","DOIUrl":"https://doi.org/10.1002/jtra.10009","url":null,"abstract":"Long-term exposure to lead has been shown to produce behavioral disturbances in human and animal models. These disturbances are shown to be associated with alterations in cholinergic and dopaminergic neurotransmission in the CNS. The present experiment was designed to study the effect of lead exposure on neurotransmitters like dopamine, serotonin, and norepinephrine and the activity of acetycholinesterase along with alterations seen in memory and locomotor functions. Lead was administrated orally at doses of 10, 50, and 200 mg/kg for a period of 12 weeks, and a dose of 50 mg/kg was also given for a period of 8 weeks on alternate days; data were collected at the end of exposure and also after recovery of 8 weeks. The most significant change noticed was the decrease in activity of the acetylcholinesterase, which did not improve following withdrawal of lead exposure. The alterations seen in various neurotransmitters remain unchanged at the end of recovery of 8 weeks. Lead exposure affected the locomotor and cognitive functions; however, following the recovery period, a significant improvement was seen in locomotor as well as cognitive behavior. The short-term memory remained unchanged following both lead exposure and after recovery. J. Trace Elem. Exp. Med. 15:131–140, 2002. © 2002 Wiley-Liss, Inc.","PeriodicalId":101243,"journal":{"name":"The Journal of Trace Elements in Experimental Medicine","volume":"15 3","pages":"131-140"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jtra.10009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72314029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Zn finger proteins: Key ways for metalloregulation 锌指蛋白：金属调控的关键途径

The Journal of Trace Elements in Experimental Medicine Pub Date : 2002-01-01 DOI: 10.1002/jtra.10007

Alexey V. Kudrin

引用次数: 2

Disorders in blood coagulation in humans occupationally exposed to mercuric vapors 职业性接触汞蒸气的人体凝血障碍

The Journal of Trace Elements in Experimental Medicine Pub Date : 2002-01-01 DOI: 10.1002/jtra.1055

Ryszard Wierzbicki, Mirosław Prażanowski, Marta Michalska, Urszula Krajewska, Wojciech P. Mielicki

引用次数: 23