Science in One Health最新文献

{"title":"Advancing knowledge of One Health in China: lessons for One Health from China's dengue control and prevention programs","authors":"Xinyu Feng , Na Jiang , Jinxin Zheng , Zelin Zhu , Junhu Chen , Lei Duan , Peng Song , Jiahui Sun , Xiaoxi Zhang , Lefei Hang , Yang Liu , Renli Zhang , Tiejian Feng , Binbin Xie , Xiaonen Wu , Zhiying Hou , Muxin Chen , Jinyong Jiang , Shizhu Li","doi":"10.1016/j.soh.2024.100087","DOIUrl":"10.1016/j.soh.2024.100087","url":null,"abstract":"<div><h3>Background</h3><div>The emergence of dengue fever has prompted significant public health responses, highlighting the need for a comprehensive understanding of One Health in addressing vector-borne diseases. China's experience in dengue control and prevention programs offers valuable insights into the successful integration of multidisciplinary strategies.</div></div><div><h3>Aims</h3><div>The review aims to: (1) systematically analyze lessons from China's dengue control and prevention programs, focusing on the integration of these efforts with the One Health approach; (2) underscore the reasons of optimizing the dengue control and prevention program; (3) highlight the alignment of China's dengue control strategies with the One Health framework; (4) contribute to global efforts in combating dengue, providing scientific evidence and strategic recommendations for other regions facing similar challenges.</div></div><div><h3>Results</h3><div>Through a comprehensive literature review and expert interviews, this study found China's approach to dengue control and prevention implemented through a hierarchical system led by the government, with collaborative efforts across multiple departments. This multi-sectoral collaboration mechanism enables the technical interventions well executed by health and disease control institutions, optimizing the integration of multiple cost-effeteness approaches, such as case management, early detection and outbreak response, reducing local transmission, and minimizing severe cases and fatalities. It was found that community participation and public health education have played a vital role in raising awareness, promoting personal protective measures, and enhancing the overall effectiveness of control efforts. The implementation of these integrated interventions has resulted in reduced dengue cases and improved capacity of outbreak response. China's dengue control strategies under the One Health framework, with focus on interdisciplinary collaboration, incorporated environmental and ecological interventions, which reduced mosquito breeding sites and improved sanitation. The findings of the review underscore the need for continuous improvement in early warning systems, scientific research, and the adoption of the One Health approach to address emerging challenges posed by climate change and the cross-border spread of infectious diseases.</div></div><div><h3>Conclusion</h3><div>China's dengue control and prevention programs provide a compelling case study for the effective application of the One Health approach. By systematically analyzing the integration of multidisciplinary strategies, this review reveals valuable lessons on optimizing public health responses to vector-borne diseases. The alignment of these strategies with One Health principles not only enhances the effectiveness of dengue control efforts in China but also offers a framework that can be adapted by other regions facing similar challenges. Ultimately","PeriodicalId":101146,"journal":{"name":"Science in One Health","volume":"3 ","pages":"Article 100087"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142707436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection and phylogenetic characterization of Rickettsia in ticks collected from leopard tortoise (Geochelone pardalis) in rural Zambia","authors":"Cynthia Sipho Khumalo ,&nbsp;Malala Mulavu ,&nbsp;Katendi Changula ,&nbsp;Benjamin Mubemba ,&nbsp;Nchimunya Bubala ,&nbsp;Anne C. Martin ,&nbsp;Innocent Billy Ng'ombwa ,&nbsp;King Shimumbo Nalubamba ,&nbsp;Simbarashe Chitanga ,&nbsp;Walter Muleya ,&nbsp;Edgar Simulundu","doi":"10.1016/j.soh.2024.100074","DOIUrl":"10.1016/j.soh.2024.100074","url":null,"abstract":"<div><p>In sub-Saharan Africa, limited studies have investigated zoonotic pathogens that may be harboured by ticks infesting reptiles such as tortoises. Here, we report the presence of pathogenic <em>Rickettsia</em> in ticks (<em>Amblyomma marmoreum</em>) collected from the leopard tortoise (<em>Geochelone pardalis</em>) in rural Zambia. Using polymerase chain reaction, 56% (49/87) of ticks were positive for the <em>Rickettsia</em> outer membrane protein (<em>ompB</em>) gene. Multi-locus sequence and phylogenetic analysis based on the <em>ompB</em>, <em>ompA</em>, and citrate synthase (<em>gltA</em>) genes showed that the ticks carried <em>R</em>. <em>africae</em>, and other <em>Rickettsia</em> spp. closely related to <em>R</em><em>.</em> <em>raoultii</em>, <em>R</em><em>.</em> <em>massiliae</em>, <em>R</em><em>.</em> <em>tamurae</em> and <em>R</em><em>.</em> <em>monacensis</em>. Given the proximity between humans, livestock, and wildlife in these habitats, there exists a considerable risk of transmission of zoonotic <em>Rickettsia</em> to human populations in this rural setting. These results call for heightened awareness and further research into the dynamics of tick-borne diseases in regions where humans and animals coexist, particularly in the context of tortoise-associated ticks as vectors. Understanding and addressing these potential disease vectors is crucial for effective public health measures and the prevention of <em>Rickettsia</em> zoonoses.</p></div>","PeriodicalId":101146,"journal":{"name":"Science in One Health","volume":"3 ","pages":"Article 100074"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949704324000131/pdfft?md5=0eefc20f9e9e56ddc6ae5f6d9ddce7c3&pid=1-s2.0-S2949704324000131-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142039680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strengthening global health resilience: Marburg virus-like particle vaccines and the One Health approach","authors":"Ram Bahadur Khadka ,&nbsp;Khimdhoj Karki ,&nbsp;Jitendra Pandey ,&nbsp;Rabin Gyawali ,&nbsp;Gautam Prasad Chaudhary","doi":"10.1016/j.soh.2024.100076","DOIUrl":"10.1016/j.soh.2024.100076","url":null,"abstract":"<div><p>The Marburg virus (MARV), belonging to the <em>Filoviridae</em> family, poses a significant global health threat, emphasizing the urgency to develop Marburg virus-like particle (VLP) vaccines for outbreak mitigation. The virus's menacing traits accentuate the need for such vaccines, which can be addressed by VLPs that mimic its structure safely, potentially overcoming past limitations. Early Marburg vaccine endeavors and their challenges are examined in the historical perspectives section, followed by an exploration of VLPs as transformative tools, capable of eliciting immune responses without conventional risks. Noteworthy milestones and achievements in Marburg VLP vaccine development, seen through preclinical and clinical trials, indicate potential cross-protection. Ongoing challenges, encompassing durability, strain diversity, and equitable distribution, are addressed, with proposed innovations like novel adjuvant, mRNA technology, and structure-based design poised to enhance Marburg VLP vaccines. This review highlights the transformative potential of Marburg VLPs in countering the virus, showcasing global collaboration, regulatory roles, and health equity for a safer future through the harmonious interplay of science, regulation, and global efforts.</p></div>","PeriodicalId":101146,"journal":{"name":"Science in One Health","volume":"3 ","pages":"Article 100076"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949704324000155/pdfft?md5=58d1a104b216cf2fd28b5b255f4989ad&pid=1-s2.0-S2949704324000155-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142163150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The synergies between international health regulations and One Health in safeguarding global health security","authors":"Abdifetah Mohamed","doi":"10.1016/j.soh.2024.100078","DOIUrl":"10.1016/j.soh.2024.100078","url":null,"abstract":"<div><div>The World Health Organization established International Health Regulations (IHRs) to give nations a legal framework for preventing, identifying, and responding to public health threats of international concern. On the other hand, One Health advocates for integrated approaches to health risks, acknowledging the interdependence of human, animal, and ecosystem health. By integrating these frameworks, stakeholders can leverage their respective strengths to enhance surveillance, early detection, and response mechanisms, as well as promote sustainable development and resilience against emerging health threats. This article explores the shared objectives, interconnectedness of health systems, collaborative mechanisms, and capacity-building initiatives that indicate the synergistic effects of IHRs and One Health in safeguarding global health security.</div></div>","PeriodicalId":101146,"journal":{"name":"Science in One Health","volume":"3 ","pages":"Article 100078"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How far has the globe gone in achieving One Health? Current evidence and policy implications based on global One Health index","authors":"Qiyu Zhang ,&nbsp;Jingshu Liu ,&nbsp;Lefei Han ,&nbsp;Xinchen Li ,&nbsp;Chensheng Zhang ,&nbsp;Zhaoyu Guo ,&nbsp;Anqi Chao ,&nbsp;Chenxi Wang ,&nbsp;Erya Wan ,&nbsp;Fumin Chen ,&nbsp;Hanqing Zhao ,&nbsp;Jiaxin Feng ,&nbsp;Jingbo Xue ,&nbsp;Lulu Huang ,&nbsp;Jin Chen ,&nbsp;Zhishan Sun ,&nbsp;Zile Cheng ,&nbsp;Jingxian Yin ,&nbsp;Zhengze He ,&nbsp;Liangyu Huang ,&nbsp;Xiao-Nong Zhou","doi":"10.1016/j.soh.2024.100064","DOIUrl":"https://doi.org/10.1016/j.soh.2024.100064","url":null,"abstract":"<div><h3>Background</h3><p>In the 21st century, as globalization accelerates and global public health crises occur, the One Health approach, guided by the holistic thinking of human-animal-environment and emphasizing interdisciplinary collaboration to address global health issues, has been strongly advocated by the international community. An immediate requirement exists for the creation of an assessment tool to foster One Health initiatives on both global and national scales.</p></div><div><h3>Methods</h3><p>Built upon extensive expert consultations and dialogues, this follow-up study enhances the 2022 global One Health index (GOHI) indicator system. The GOHI framework is enriched by covering three indices, e.g. external drivers index (EDI), intrinsic drivers index (IDI), and core drivers index (CDI). The comprehensive indicator system incorporates 13 key indicators, 50 indicators, and 170 sub I-indicators, utilizing a fuzzy analytic hierarchy process to ascertain the weight for each indicator. Weighted and summed, the EDI, IDI, and CDI scores contribute to the computation of the overall GOHI 2022 score. By comparing the ranking and the overall scores among the seven regions and across 160 countries/territories, we have not only derived an overall profile of the GOHI 2022 scores, but also assessed the GOHI framework. We also compared rankings of indicators and sub I-indicators to provide greater clarity on the strengths and weaknesses of each region within the One Health domains.</p></div><div><h3>Results</h3><p>The GOHI 2022 performance reveals significant disparities between countries/territories ranged from 39.03 to 70.61. The global average score of the GOHI 2022 is 54.82. The average score for EDI, IDI, and CDI are 46.57, 58.01, and 57.25, respectively. In terms of global rankings, countries from North America, Europe and Central Asia, East Asia and Pacific present higher scores. In terms of One Health domains of CDI, the lowest scores are observed in antimicrobial resistance (median: 43.09), followed by food security (median: 53.78), governance (median: 54.77), climate change (median: 64.12) and zoonotic diseases (median: 69.23). Globally, the scores of GOHI vary spatially, with the highest score in North America while lowest in sub-Saharan Africa. In addition, evidence shows associations between the socio–demographic profile of countries/territories and their GOHI performance in certain One Health scenarios.</p></div><div><h3>Conclusion</h3><p>The objective of GOHI is to guide impactful strategies for enhancing capacity building in One Health. With advanced technology and an annually updated database, intensifying efforts to refine GOHI's data-mining methodologies become imperative. The goal is to offer profound insights into disparities and progressions in practical One Health implementation, particularly in anticipation of future pandemics.</p></div>","PeriodicalId":101146,"journal":{"name":"Science in One Health","volume":"3 ","pages":"Article 100064"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949704324000039/pdfft?md5=23c7efab80afed9b864b08486e590e15&pid=1-s2.0-S2949704324000039-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140339463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global burden of enteric infections related foodborne diseases, 1990–2021: findings from the Global Burden of Disease Study 2021","authors":"Tianyun Li ,&nbsp;Ne Qiang ,&nbsp;Yujia Bao ,&nbsp;Yongxuan Li ,&nbsp;Shi Zhao ,&nbsp;Ka Chun Chong ,&nbsp;Xiaobei Deng ,&nbsp;Xiaoxi Zhang ,&nbsp;Jinjun Ran ,&nbsp;Lefei Han","doi":"10.1016/j.soh.2024.100075","DOIUrl":"10.1016/j.soh.2024.100075","url":null,"abstract":"<div><h3>Background</h3><p>Understanding the global burden of enteric infections is crucial for prioritizing control strategies for foodborne and waterborne diseases. This study aimed to assess the global burden of enteric infections in 2021 and identify risk factors from One Health aspects.</p></div><div><h3>Methods</h3><p>Leveraging the Global Burden of Disease (GBD) 2021 database, the incidence, disability-adjusted life years (DALYs), and deaths of enteric infections and the subtypes were estimated, including diarrheal diseases, typhoid and paratyphoid fever, invasive non-typhoidal <em>Salmonella</em> (iNTS) infections, and other intestinal infectious diseases. The estimates were quantified by absolute number, age-standardized incidence rate (ASIR), age-standardized mortality rate (ASMR) and age-standardized DALY rate with 95% uncertainty intervals (<em>UI</em>s). Thirteen pathogens and three risk factors associated with diarrheal diseases were analyzed.</p></div><div><h3>Results</h3><p>In 2021, the global age-standardized DALY rate of enteric infections was 1020.15 per 100,000 popultion (95% <em>UI</em>: 822.70–1259.39 per 100,000 population) with an estimated annual percentage change (EAPC) of −4.11% (95% confidence interval: −4.31% to −3.90%) in 1990–2021. A larger burden was observed in regions with lower Socio-demographic index (SDI) levels. Diarrheal disease was the most serious subtype with Western Sub-Saharan Africa exhibiting the highest age-standardized DALY rate (2769.81 per 100,000 population, 95% <em>UI</em>: 1976.80–3674.41 per 100,000 population). Children under 5 and adults over 65 years suffered more from diarrheal diseases with the former experiencing the highest global age-standardized DALY rate (9382.46 per 100,000 population, 95% <em>UI</em>: 6771.76–13,075.12 per 100,000 population). Rotavirus remained the leading cause of diarrheal diseases despite a cross-year decline in the observed age-standardized DALY rate. Unsafe water, sanitation, and handwashing contributed most to the disease burden.</p></div><div><h3>Conclusion</h3><p>The reduced burden of enteric infections suggested the effectiveness of previous control strategies; however, more efforts should be made in vulnerable regions and populations through a One Health approach.</p></div>","PeriodicalId":101146,"journal":{"name":"Science in One Health","volume":"3 ","pages":"Article 100075"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949704324000143/pdfft?md5=c0459424185bbcb2edd4a1d74042b82c&pid=1-s2.0-S2949704324000143-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of improper municipal solid waste management on fostering One Health approach in Ethiopia — challenges and opportunities: A systematic review","authors":"Tsegay Kahsay Gebrekidan ,&nbsp;Niguse Gebru Weldemariam ,&nbsp;Hagos Degefa Hidru ,&nbsp;Gebremariam Gebrezgabher Gebremedhin ,&nbsp;Abraha Kahsay Weldemariam","doi":"10.1016/j.soh.2024.100081","DOIUrl":"10.1016/j.soh.2024.100081","url":null,"abstract":"<div><div>Improper disposal of solid waste, predominantly illegal dumping, can lead to severe air and water pollution, land degradation, climate change, and health hazards due to the persistence of hazardous materials. As a result, it is threatening public and animal health, environmental sustainability, and economic development. The One Health approach, which acknowledges the interconnectedness of human, animal, and environmental health, offers a comprehensive solution. This systematic review examines the impact of improper municipal solid waste on fostering One Health approaches at the national level of Ethiopia by identifying key challenges and opportunities. Publications were retrieved from peer-reviewed, indexed journal publications, government documents (policies, proclamations, regulations, and guidelines), and credible non-governmental organization publications from selected electronic databases (Google scholar, PubMed, EMBASE, Global Health, Web of Science, etc.), and governmental offices. Despite efforts to advance the One Health approach in Ethiopia through the formation of the National One Health Steering Committee and technical working groups, implementation is hindered by challenges such as poor sectoral integration, insufficient advocacy, financial constraints, and limited research. These challenges contribute to worsening zoonotic and infectious diseases and environmental issues due to inadequate solid waste management. Nonetheless, opportunities exist through One Health integration via holistic programs, interdisciplinary collaboration, community engagement, policy enhancement, institutional capacity building, and public-private partnerships. Therefore, enhancing sectoral integration and increasing advocacy efforts and securing financial support is necessary to back waste management initiatives and related research. Further research is crucial to understand the impact of solid waste management and the potential benefits of the One Health approach in Ethiopia.</div></div>","PeriodicalId":101146,"journal":{"name":"Science in One Health","volume":"3 ","pages":"Article 100081"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends in epidemiological characteristics and etiologies of diarrheal disease in children under five: an ecological study based on Global Burden of Disease study 2021","authors":"Chu Chu ,&nbsp;Guobing Yang ,&nbsp;Jian Yang ,&nbsp;Defeng Liang ,&nbsp;Ruitao Liu ,&nbsp;Guanhua Chen ,&nbsp;Jichun Wang ,&nbsp;Guisheng Zhou ,&nbsp;Hongli Wang","doi":"10.1016/j.soh.2024.100086","DOIUrl":"10.1016/j.soh.2024.100086","url":null,"abstract":"<div><h3>Background</h3><div>Diarrhea remains a significant health threat to children under five years of age. The study aims to systematically elucidate the global burden of diarrhea in children, providing scientific insights for effective prevention and control strategies.</div></div><div><h3>Methods</h3><div>The data from the Global Burden of Disease (GBD) 2021 study was analyzed to assess the trends in incidence, prevalence, mortality, and disability-adjusted life years (DALYs) of diarrhea in children under five years across the globe, 21 geographical region, and 204 countries and territories, stratified by age group, sex, and socio-demographic index (SDI) levels. The trend of the disease burden for childhood diarrhea from 1990 to 2021 was described and estimated using the average annual percent change (AAPC), and a Bayesian age-period-cohort (BAPC) model was employed to predict the future burden of diarrhea in children.</div></div><div><h3>Results</h3><div>From 1990 to 2021, there was a significant decline in the global burden of diarrhea among children under five years of age. The AAPC for incidence (−4092.18, 95% confidence interval [<em>CI</em>]: −4224.60 to −3959.76), prevalence (−70.98, 95% <em>CI</em>: −72.67 to −69.28), mortality (−6.89, 95% <em>CI</em>: −6.95 to −6.83), and DALYs rate (−621.79, 95% <em>CI</em>: −627.20 to −616.38) of diarrhea in children all showed a marked downward trend. Diarrheal incidence (<em>r</em> = −0.782, <em>P</em> &lt; 0.001), prevalence (<em>r</em> = −0.777, <em>P</em> &lt; 0.001), mortality (<em>r</em> = −0.908, <em>P</em> &lt; 0.001), and DALYs rate (<em>r</em> = −0.904, <em>P</em> &lt; 0.001) were negatively correlated with the SDI. Between 2022 and 2035, the global incidence, prevalence, and mortality rates of diarrhea in children under five years are projected to continue declining. The leading causes of diarrheal mortality in children include wasting, underweight, and non-exclusive breastfeeding. Rotavirus remains the predominant pathogen associated with diarrhea-related mortality rate and DALY rate.</div></div><div><h3>Conclusion</h3><div>Although the global burden of diarrhea in children under five has steadily declined, it remains a significant health threat. Rotavirus is the leading pathogen, highlighting the importance of expanding rotavirus vaccination. Additionally, improving nutritional status, increasing exclusive breastfeeding rates, and enhancing access to sanitation and clean drinking water are crucial measures that, when widely implemented, can effectively reduce the health risks posed by diarrhea in children.</div></div>","PeriodicalId":101146,"journal":{"name":"Science in One Health","volume":"3 ","pages":"Article 100086"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142660396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protocol for detection of pathogenic enteric RNA viruses by regular monitoring of environmental samples from wastewater treatment plants using droplet digital PCR","authors":"Ram Kumar Nema ,&nbsp;Surya Singh ,&nbsp;Ashutosh Kumar Singh ,&nbsp;Devojit Kumar Sarma ,&nbsp;Vishal Diwan ,&nbsp;Rajnarayan R. Tiwari ,&nbsp;Rajesh Kumar Mondal ,&nbsp;Pradyumna Kumar Mishra","doi":"10.1016/j.soh.2024.100080","DOIUrl":"10.1016/j.soh.2024.100080","url":null,"abstract":"<div><h3>Background</h3><div>The present comprehensive protocol is focused on the detection of pathogenic enteric RNA viruses, explicitly focusing on norovirus genogroup Ⅱ (GⅡ), astrovirus, rotavirus, Aichi virus, sapovirus, hepatitis A and E viruses in wastewater treatment plants through droplet digital PCR (ddPCR). Enteric viruses are of significant public health concern, as they are the leading cause of diseases like gastroenteritis. Regular monitoring of environmental samples, particularly from wastewater treatment plants, is crucial for early detection and control of these viruses. This research aims to improve the understanding of the prevalence and dynamics of enteric viruses in urban India and will serve as a model for similar studies in other regions. Our protocol's objective is to establish a novel ddPCR-based methodology for the detection and molecular characterization of enteric viruses present in wastewater samples sourced from Bhopal, India. Our assay is capable of accurately quantifying virus concentrations without standard curves, minimizing extensive optimization, and enhancing sensitivity and precision, especially for low-abundance targets.</div></div><div><h3>Methods</h3><div>The study involves fortnightly collecting and analyzing samples from nine wastewater treatment plants over two years, ensuring comprehensive coverage and consistent data. Our study innovatively applies ddPCR to simultaneously detect and quantify enteric viruses in wastewater, a more advanced technique. Additionally, we will employ next-generation sequencing for detailed viral genome identification in samples tested positive for pathogenic viruses.</div></div><div><h3>Conclusion</h3><div>This study will aid in understanding these viruses’ genetic diversity and mutation rates, which is crucial for developing tailored intervention strategies. The findings will be instrumental in shaping public health responses and improving epidemiological surveillance, especially in localities heaving sewage networks.</div></div>","PeriodicalId":101146,"journal":{"name":"Science in One Health","volume":"3 ","pages":"Article 100080"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the global reach of African anthrax research: A bibliometric study","authors":"Ishaku Leo Elisha ,&nbsp;Arieri Onikisateinba ,&nbsp;Ganih Saidu Joel ,&nbsp;Pam Dachung Luka ,&nbsp;Barde Israel Joshua ,&nbsp;Shuaibu Hafsat Jagab ,&nbsp;Rabi Ahmed ,&nbsp;Habibu Haliru ,&nbsp;Sa’adatu Aliyu Abubakar ,&nbsp;Sunday Makama ,&nbsp;Ngulukun Samuel Sati ,&nbsp;Maryam Muhammad","doi":"10.1016/j.soh.2023.100052","DOIUrl":"https://doi.org/10.1016/j.soh.2023.100052","url":null,"abstract":"<div><p>Anthrax is a zoonotic bacterial disease caused by <em>Bacillus anthracis</em>. It poses significant threat to humans through contact with infected animals or their by-products. Concerns arise from its long-lasting spore viability and lethality, fuelling its biowarfare potential. Recent anthrax outbreaks across multiple African nations prompted this bibliometric study. The aim of the study was to assess the contributions of African countries, institutions, authors, research funding, and collaborations, while identifying research trends and gaps. We conducted an extensive bibliometric analysis of anthrax-related research publications in Africa from 1923 to 2023, utilizing the Scopus database and VOSviewer. The study covered 364 publications from 32 African countries, accumulating 5,636 citations at an average of 15.5 citations per article, with research articles comprising 88.5% of the corpus. The publication growth rate from 1923 to 2023 was modest at 8.3%, indicating gradual advancement. Notably, there was a significant surge in publications between 2011 and 2023, accounting for 73.1% of total publications. The African research contributions, were categorized into five thematic focuses: ecological dynamics and host interactions, human–livestock anthrax interface, molecular insights into bacterial activity and treatment strategies, collaborative approaches for zoonotic disease prevention, and antibody response and vaccination strategies. Leading institutional contributors included the University of Pretoria and the University of KwaZulu-Natal. Collaborations extended globally to 35 non-African countries, with significant involvement from the United States, United Kingdom, and Germany. Strong African partnerships, especially between Kenya, Nigeria, and South Africa, emerged. The top 10 cited papers explored diverse aspects, including disease impact on wildlife and innovative control strategies, underscoring the importance of multidisciplinary approaches. South Africa played a prominent role, contributing 95 publications and securing funding from various sources, including the National Research Foundation. Collaborations with global institutions highlighted its commitment. This study unveils the dynamic landscape of anthrax research in Africa, emphasizing the pivotal role of collaboration, multidisciplinary One Health approaches, and global partnerships in enhancing research outcomes. Ongoing research and practical solutions for human and animal health remain imperative.</p></div>","PeriodicalId":101146,"journal":{"name":"Science in One Health","volume":"3 ","pages":"Article 100052"},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S294970432300046X/pdfft?md5=788907cdc6570262d882540c3278ae8c&pid=1-s2.0-S294970432300046X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138467662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}