Plant Nano Biology最新文献

{"title":"Interactive effect of biogenic nanoparticles and UV-B exposure on physio-biochemical behavior and secondary metabolism of Artemisia annua L","authors":"Deepika Tripathi ,&nbsp;Apoorva ,&nbsp;Niraj Kumar Goswami ,&nbsp;Shashi Pandey-Rai","doi":"10.1016/j.plana.2024.100097","DOIUrl":"10.1016/j.plana.2024.100097","url":null,"abstract":"<div><div>Biosynthesized silver nanoparticles (AgNPs) are key nanomaterials with unique physio-chemical characteristics and diverse applications. Their strong absorption potential and antibacterial activity make them useful for agriculture, medicine and other industries. AgNPs boost plant growth and metabolism, especially under stress. However, the combined effects of AgNPs and UV-B exposure on plants are unknown. To elucidate the interactive effects of biosynthesized silver nanoparticles (AgNPs) and exposure of ultraviolet B (UV-B) on plant growth and metabolic processes, this study assessed the response of <em>Artemisia annua</em> under controlled <em>in vitro</em> conditions. In total, eight sets of plants were used with the alone/combined treatment of AgNPs and UV-B. For this purpose, spherical and averaged ∼ 31.8 nm in size AaAgNPs were synthesized. The photosynthetic pigments were calculated maximum with the alone treatment of 0.5 mg L⁻¹ AaAgNPs and combined treatment of 0.5 mg L⁻¹ AaAgNPs with 3 h UV-B, respectively. The results evidenced that the co-exposure of AaAgNPs and UV-B led to a significant balance in ROS production of <em>A. annua</em>; as well as improved antioxidative enzyme activity. Fluorescence and scanning electron microscopic (SEM) analysis indicated the enhancement of glandular trichomes (GT) area and density with the combined treatment of AaAgNPs and 3 h UV-B. In accordance with correlation between microscopic GT results, high concentration of artemisinin and up-regulation of related transcripts were found in <em>A. annua</em> plants treated with low concentrations of AaAgNPs and UV-B. Thus, it may be inferred that two distinct plant growth modulators, namely low-concentration biosynthesized AgNPs and short-term UV-B exposure, can enhance the physio-biochemical characteristics and production of secondary metabolites (specially artemisinin) in A. annua synergistically.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100097"},"PeriodicalIF":0.0,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142530301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-priming: Improving plant nutrition to support the establishment of sustainable agriculture under heavy metal stress","authors":"Mohammad Faizan ,&nbsp;Pooja Sharma ,&nbsp;Haider Sultan ,&nbsp;Pravej Alam ,&nbsp;Shafaque Sehar ,&nbsp;Vishnu D. Rajput ,&nbsp;Shamsul Hayat","doi":"10.1016/j.plana.2024.100096","DOIUrl":"10.1016/j.plana.2024.100096","url":null,"abstract":"<div><div>Heavy metals (HMs) have become a severe problem for all living organisms, including plants, because of their unprecedented bioaccumulation and biomagnification in the environment. When exposed to hazardous quantities of HMs, various essential cellular macromolecules, including DNA and nuclear proteins, can interact with HMs, causing an overproduction of reactive oxygen species (ROS). Recently, several techniques have been used to ameliorate HM toxicity, including nano-priming, which effectively modulates plant physiological and biochemical responses under HM stress. This review summarizes the literature on the effectiveness of nano-priming for boosting germination, growth, photosynthetic efficiency, biomass accumulation, and crop yield. Additionally, information regarding the application of nano-priming to reduce HM toxicity in plants is reviewed. Future research prospects are indicated by highlighting the knowledge gaps in the current literature and underlining the need optimize and validate nano-priming techniques and their physiological effects on plants.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100096"},"PeriodicalIF":0.0,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142444558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of cobalt oxide nanosheets using Withania somnifera root extract for degradation of organic pollutants","authors":"Chinky Gangwar ,&nbsp;Saloni Sahu ,&nbsp;Ritik Jaiswal ,&nbsp;Nisha Gangwar ,&nbsp;Ashish Soni","doi":"10.1016/j.plana.2024.100099","DOIUrl":"10.1016/j.plana.2024.100099","url":null,"abstract":"<div><div>In today’s scenario, material science emerges as pivotal players, as it is an emerging and fast-growing interdisciplinary field. Nanomaterials are increasingly popular among researchers due to their unique physicochemical properties and multifaceted applications. Recently, researchers are exploring more sustainable approaches for the synthesis of nanomaterials due to its numerous advantages. In this context, present investigation reports an approach for the synthesis of cobalt oxide nanosheets (Co₃O₄ NSs). This approach leverages environmentally friendly and sustainable methods, minimizing the use of hazardous chemicals and reducing energy consumption. Hence it involved <em>Withania somnifera</em> (ashwagandha) root extract as a greener reductant as well as stabilizing agent. The synthesized Co₃O₄ NSs were thoroughly analyzed using various techniques, including ultraviolet-visible (UV–vis) spectroscopy, fourier-transform infrared spectroscopy (FTIR), powder x-ray diffraction (PXRD), and field emission scanning electron microscopy (FE-SEM). A sharp absorption peak at 252 nm with a tail towards higher wavelength reveal the formation of Co₃O₄ NSs. The diffraction pattern reveals a face centered cubic structure of Co₃O₄ NSs. Morphological studies confirmed the substantial surface area of Co₃O₄ NSs which enable us to perform the catalytic degradation of azo dye, i.e., methyl orange. It provides that 10 mg of Co₃O₄ NSs is sufficient to degrade a 10 ppm aqueous methyl orange solution by 75.82 % in the dark and by 96.12 % under sunlight exposure. Thus, this study offers an excellent pathway for the synthesis of Co₃O₄ NSs and demonstrates their potential as a promising material for future catalytic applications.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100099"},"PeriodicalIF":0.0,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142444557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional and transcriptomic insights into plant response to arginine-functionalized nanohydroxyapatite treatment","authors":"Chinenye L. Izuegbunam,&nbsp;Beate Wone,&nbsp;Bernard W.M. Wone","doi":"10.1016/j.plana.2024.100093","DOIUrl":"10.1016/j.plana.2024.100093","url":null,"abstract":"<div><div>The manipulation of the plant genome is essential for elucidating gene functions in plants and advancing the development of climate-resistant crops. We have demonstrated that a nanohydroxyapatite (nHA)-mediated gene delivery system is effective in the transformation of reporter genes into six plant species. Despite the potential advantages of nHA-mediated biomolecule delivery and its application as fertilizers, phytotoxicity concerns necessitate additional studies. While initial findings suggest the beneficial effects of nHA as a nanofertilizer at specific concentrations, a thorough investigation into its bioactivity is warranted. This study reports the bioactivity of nHA on two model plants, including a crop species, and examines the global gene expression alterations in <em>Arabidopsis thaliana</em>. Treatment of seeds and seedlings with arginine-functionalized nHA (R-nHA) at concentrations at 50, 200 and 500 µg/ml led to accelerated germination in <em>Arabidopsis</em>, an effect not observed in <em>Nicotiana benthamiana</em>. Additionally, R-nHA did not affect root growth in either model species but significantly promoted root and leaf growth in <em>Triticum aestivum</em>. Transcriptomic analysis revealed minimal transcriptional changes in <em>Arabidopsis</em> treated with R-nHA compared to a water control, including activated phytohormone signaling pathways and stress-responsive genes. Salicylic acid (SA) has been identified as a pivotal phytohormone in initiating stress resistance in response to R-nHA exposure in <em>Arabidopsis</em>, highlighting its essential role in plant defense mechanisms against both biotic and abiotic stresses. In summary, this study showed that R-nHA accelerates germination and promotes plant growth with minimal transcriptional changes, thereby laying the groundwork for the use of nHA in plant genome manipulations. This research indicates that nHAs are highly biocompatible for plant bionanotechnology applications.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100093"},"PeriodicalIF":0.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidation of biochemical and physiological modulations in Triticum aestivum induced by green synthesized nitrogen-enriched zinc nano-complexes","authors":"Zari Shiran ,&nbsp;Sedigheh Esmaeilzadeh Bahabadi ,&nbsp;Zohreh Razmara ,&nbsp;Kavitha Beluri ,&nbsp;Nusrat Easmin ,&nbsp;Amirhossein Mahdaviarab ,&nbsp;Hamidreza Sharifan","doi":"10.1016/j.plana.2024.100094","DOIUrl":"10.1016/j.plana.2024.100094","url":null,"abstract":"<div><div>This study investigates the efficacy of a green synthesized nitrogen-rich zinc complex (Zn-NC) using quinoline (C₉H₇N) as the nitrogen-rich substrate to enhance growth and biochemical properties in wheat (<em>Triticum aestivum</em>). The performance of Zn-NC was compared to standard zinc oxide nanoparticles (ZnO-NPs). Both Zn-NC and ZnO-NPs were characterized using scanning electron microscopy (SEM) and X-ray diffraction (XRD), and dynamic light scattering (DLS). Three concentrations (100, 200, and 500 ppm) of each compound, along with a control, were applied to local soil samples (n=3). The physiological (biomass, elongation) and biochemical effects (chlorophyll, carotenoids, flavonoids, and phenols) on wheat were investigated. Potential phytotoxic effects were evaluated to establish the biostimulants' safety thresholds. Plants treated with green Zn-NC showed an average increase in shoot length of 25 % compared to the control group. The chlorophyll content in plants treated with ZnO-NPs increased by 18 %, while those treated with green Zn-NC increased by 12 % compared to control. Application of ZnO-NPs resulted in a 30 % increase in total yield, whereas green Zn-NC treatment led to a 22 % yield increase. The root biomass of plants treated with ZnO-NPs increased by 28 %, and those treated with green Zn-NC saw a 20 % increase compared to controls. Based on the optimization of overall results, the ZnO NPs showed phytotoxic effects at concentrations above 200 ppm, while green Zn-NC exhibited no significant phytotoxicity even at concentrations up to 300 ppm. This study delineates the optimal concentrations of Zn-NC and ZnO-NPs that can enhance nutrient delivery and yield in cereal crops while mitigating phytotoxic risks. The findings provide valuable insights into applying nano-biostimulants in agroecosystems, highlighting their potential to improve productivity and sustainability in agriculture.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100094"},"PeriodicalIF":0.0,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial Nanotechnology for Plant Science and Agriculture","authors":"Hena Dhar,&nbsp;Javaid Akhter Bhat,&nbsp;Ulhas Kadam,&nbsp;Rupesh Deshmukh","doi":"10.1016/j.plana.2024.100088","DOIUrl":"10.1016/j.plana.2024.100088","url":null,"abstract":"","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"9 ","pages":"Article 100088"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773111124000317/pdfft?md5=afdd7501ebb78c3573724a9713854edd&pid=1-s2.0-S2773111124000317-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141979018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Cymbopogon khasianus essential oil infused chitosan nanoemulsion for mitigation of Aspergillus flavus and aflatoxin B1 contamination in stored Syzygium cumini seeds","authors":"Jitendra Prasad ,&nbsp;Monisha Soni ,&nbsp;Arati Yadav ,&nbsp;Kishor Kumar Paul ,&nbsp;Manish Kumar Jha ,&nbsp;Umakant Banjare ,&nbsp;Somenath Das ,&nbsp;Nawal Kishore Dubey ,&nbsp;Abhishek Kumar Dwivedy","doi":"10.1016/j.plana.2024.100085","DOIUrl":"10.1016/j.plana.2024.100085","url":null,"abstract":"<div><p>This research developed a novel chitosan nanoemulsion infused with <em>Cymbopogon khasianus</em> essential oil (CKEO-ChNe) and characterized through Dynamic light scattering, Atomic force microscopy, scanning electron microscopy, fourier transform infrared spectroscopy, and x-ray diffractometry analyses followed by its efficacy testing against fungi and aflatoxin B₁ contamination in <em>Syzygium cumini</em> seeds. The CKEO-ChNe inhibited <em>Aspergillus flavus</em> and aflatoxin B₁ (AFB₁) production at 0.32 and 0.28 µL/mL with enhanced antioxidant activity and controlled delivery strategies. The inhibition of methylglyoxal and ergosterol biosynthesis, leakage of ions and molecular interaction of geraniol with Ver-1 (Versicolorin A dehydrogenase) and Omt-A (O-methyl transferase) proteins suggested the antifungal and anti-AFB₁ mechanism of action. Further, the <em>in situ</em> protection of <em>Syzygium cumini</em> seeds against fungi, AFB₁ contamination and lipid peroxidation (p&lt;0.05) without altering the sensory characteristics, and favorable safety profile in mammalian model recommend the potentiality of encapsulated CKEO nanoemulsion as smart nano-fungitoxic preservative in agricultural and pharmaceutical industries.</p></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"9 ","pages":"Article 100085"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773111124000287/pdfft?md5=706f8ab3d30db229e95594b129fef168&pid=1-s2.0-S2773111124000287-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141843433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of calcium-based nanofertilizer and its efficacy towards reduction of oxidative stress and fluoride uptake in rice (Oryza sativa L.)","authors":"Rajesh Koley,&nbsp;Naba Kumar Mondal","doi":"10.1016/j.plana.2024.100087","DOIUrl":"10.1016/j.plana.2024.100087","url":null,"abstract":"<div><p>The phytotoxicity of fluoride and its build-up in agricultural plants and subsequently the entry into the food chain is a serious threat to human health. The present study highlighted the green synthesis of calcium oxide nanoparticles (CaO NPs) and characterization using UV-Vis spectrophotometer, TEM, SEM, EDX, XRD, and FTIR. Further, synthesized CaO NPs (0, 10, and 50 mg/L) were applied on fluoride-stressed (10 mg/L) rice seedlings to check its possible ameliorative effects towards growth and fluoride accumulation in different parts of rice seedlings. Characterization revealed that nanoparticles were crystalline (46.72 %) and spherical in shape, with an average diameter of 20–25 nm. Results of the seedling growth analysis revealed that CaO NPs inhibited the translocation of fluoride in rice plants, which in turn decreased the phytotoxicity caused by fluoride, including lipid peroxidation and chlorosis, and enhanced the overall growth of seedlings. The co-exposure of CaO NPs with fluoride also showed a reduction in the fluoride-induced oxidative stress, as demonstrated by lower MDA, O₂^•- contents, and activity of antioxidant enzymes (CAT, SOD, and POD) as compared to fluoride treatment alone. The application of CaO NPs also restored potassium content in seedlings grown under fluoride stress. Furthermore, the highest reduction of fluoride accumulation by 65 and 76 % in roots and shoots was recorded at 50 mg/L of CaO NPs treatment, respectively. Therefore, the present study clearly indicated the ameliorative potential of CaO NPs towards fluoride stress in rice. However, a field study is needed to establish the social acceptance of this valuable nanofertilizer in fluoride-contaminated areas.</p></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"9 ","pages":"Article 100087"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773111124000305/pdfft?md5=75f30dc28a1e2e8b46ebf93906cce32f&pid=1-s2.0-S2773111124000305-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141850439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant-endosymbiont mediated synthesis of silver nanobactericides with bioautography-guided partial purification of novel 1,2-benzenedicarboxylic acid, decyl octyl ester","authors":"H.K. Ranjini ,&nbsp;K. Manju ,&nbsp;Baker Syed ,&nbsp;Raj. S. Niranjan ,&nbsp;K.P. Kripali ,&nbsp;Akhila ,&nbsp;V. Apeksha ,&nbsp;Vinayak Hegde Shreya ,&nbsp;Prasad.M. N. Nagendra ,&nbsp;H. Shayista ,&nbsp;P. Ashwini","doi":"10.1016/j.plana.2024.100089","DOIUrl":"10.1016/j.plana.2024.100089","url":null,"abstract":"<div><p>In the present study, synthesis of silver nanobactericides was achieved from <em>Curcuma longa</em> L. and its endosymbiont for their antimicrobial activity. The nanobactericides exhibited notable antimicrobial potential against <em>Escherichia coli</em> and <em>Staphylococcus aureus</em>, as determined through broth dilution and minimum inhibitory concentration which ranged between 2.5 and 5 mg/mL. Characterization of nanobactericides revealed polydispersity with average size of 80 nm and crystalline nature showed distinct peaks. The Fourier transform infrared (FTIR) analysis revealed presence of exhibited peaks at 3362.24 cm⁻¹ (hydroxyl group), 1637.96 cm⁻¹ (carbonyl group), 1377.25 cm⁻¹ (alkane group), and 635.25 cm⁻¹ (alkyl group) which participated in the synthesis of nanobactericides. Endosymbiont crude extracts subjected to partial purification through thin-layer chromatography, and bioautography-guided fractionation identified an active compound at <em>R</em>_<em>f</em> 0.7 which showed clear zone of inhibition. The minimum inhibitory concentration of metabolite fraction was found to be 0.625 mg/mL against both the test pathogens. The dye degradation potential of nanobactericides was successful 81.27 % of degradation was achieved with safranin treated with silver nanobactericides from endosymbiont. Subsequently, nanobactericides synthesized from plants showed 59.88 % highest degradation with methylene blue. The profiling of metabolite was carried out with gas chromatography-mass spectrometry to identify as a novel metabolite, 1,2-benzenedicarboxylic acid, decyl octyl ester, showing antimicrobial potential against tested pathogens. The identified metabolite molecular formula was found to be C₂₆H₄₂O₄ with molecular weight of 418. These promising results, especially for the scarcely reported compound, contribute to the understanding of plant-endosymbiont-synthesized nanobactericides with significant antimicrobial properties. The molecular analysis revealed the endosymbiont's affinity to <em>Pseudomonas aeruginosa</em>, and its sequence has been deposited in GenBank (Accession number OR984817). The study concludes with importance of nanobactericides from plant-endosymbiont consortium, highlighting their antimicrobial efficacy against human and phytopathogens.</p></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"9 ","pages":"Article 100089"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773111124000329/pdfft?md5=5144cd94924a1f3ca3b8d870d46285f6&pid=1-s2.0-S2773111124000329-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141998283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of Inonotus rickii metabolite-loaded chitosan nanoparticles and its biological activities","authors":"Pallavi Champaneria ,&nbsp;Hiralal Sonawane ,&nbsp;Deepak Shelke ,&nbsp;Mahadev Chambhare ,&nbsp;Kamalakar More ,&nbsp;Siddharam Math ,&nbsp;Balkrishna Zaware","doi":"10.1016/j.plana.2024.100092","DOIUrl":"10.1016/j.plana.2024.100092","url":null,"abstract":"<div><p><em>Inonotus rickii,</em> a mushroom fungus classified as a Hyamenochetae, is commonly observed in the Western Ghats region of Maharashtra, India. The metabolites extracted from this mushroom exhibit various biological activities. On the other side, currently chitosan nanoparticles emerging as an effective nanocarrier for targeted treatments. Therefore in the present study nanoparticles from biopolymer chitosan loaded with <em>I. rickii</em> metabolites of different solvent extract were synthesized and their antioxidant, antibacterial and anticancer potential were evaluated. The synthesized NPs were spherical in shape, and the average size for pure chitosan nanoparticles (CHI-NPs) was 58.02 nm, <em>I. rickii</em> aqueous CHI-NPs (U2-Aq-CHI-NPs) 62.70 nm, <em>I. rickii</em> acetone CHI-NPs (U2-Ac-CHI-NPs) 17.11 nm, and <em>I. rickii</em> ethanol CHI-NPs (U2-Et-CHI-NPs) 27.05 nm. These <em>I. rickii</em> metabolite-loaded CHI-NPs exhibited antibacterial, antioxidant, and cytotoxic activities. The U2-Ac-CHI-NPs showed significant antibacterial actions against four bacterial strains such as <em>E. coli</em>, <em>Bacillus cereus</em>, <em>Pseudomonas otitidis</em>, and <em>Chryseobacterium</em> spp. It also showed significantly higher antioxidant activity (95.8±1.6 %). Moreover, a significant decrease in the cell viability of the HeLa cell line was noticed with a subsequent increase (1–1000 µg/mL) in the concentration of metabolite-loaded CHI-NPs. A U2-Aq-CHI-NPs lower concentration is also significant to decrease cell viability. The LC-MS analysis revealed the presence of different bioactive compounds like Hispolon, Inoscavins, Methyl inoscavins, and Phelligridins in all tested extracts of <em>I. rickii</em> supports the bioactivities. Therefore, the synergistic effect of <em>I. rickii-</em>derived metabolite-loaded CHI-NPs suggested a significant perspective for the targeted drug delivery-based treatment with diminished side effects.</p></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"9 ","pages":"Article 100092"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773111124000354/pdfft?md5=6dfde966f2a4cd1ce06c105aca536980&pid=1-s2.0-S2773111124000354-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142238428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}