Pharmacological Research - Reports最新文献

{"title":"Insights into the antibacterial activity of Limosilactobacillus fermentum curd isolate against gram-positive and gram-negative pathogenic bacteria","authors":"Suchhanda Nandi ,&nbsp;Shyamapada Mandal","doi":"10.1016/j.prerep.2025.100034","DOIUrl":"10.1016/j.prerep.2025.100034","url":null,"abstract":"<div><div>It is widely known that lactic acid bacteria (LAB), commonly found in fermented products like curd, extends health benefits and antibacterial properties, particularly in traditional Chinese medicine (TCM). In spite of the growing demand for sweet curd contained in earthen pots in our region, no research has specifically explored its unique properties. This study aims to address this gap by examining the probiotic characteristics, antibacterial activity and consumer appeal of sweet curd in earthen pots. A sweet curd sample containing in an earthen pot was microbiologically cultured to isolate LAB applying conventional methods and 16S rRNA gene sequencing. The strain thus isolated was identified to be <em>Limosilactobacillus fermentum</em> 406B, showed broad-spectrum antibacterial activity against gram-positive: <em>Staphylococcus aureus</em>, <em>Bacillus cereus</em>, <em>Streptococcus pneumoniae</em> and <em>Listeria monocytogenes</em> and gram-negative: <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em>, <em>Acinetobacter baumannii</em> and <em>Proteus vulgaris</em> bacterial pathogens. Antimicrobial property and minimum inhibitory concentration (MIC) were assessed by agar well diffusion and broth dilution method, respectively. Initial tests based on colony morphology, gram staining and biochemical assays confirmed it as a <em>Lactobacillus</em> species, with sequencing confirming its identity as <em>Limosilactobacillus fermentum</em> 406B. The strain demonstrated excellent physiological tolerance to NaCl, bile, low pH and varying temperatures, confirming its suitability as a probiotic. Additionally, safety tests showed that it was non-pathogenic, as it tested negative for gelatinase and DNase production and displayed an acceptable antibiotic susceptibility profile. With a cumulative probiotic potential (CPP) of 100 %, <em>L. fermentum</em> 406B holds significant promise as a probiotic strain. These results underscore the strain’s potential not just in TCM but also as a prospective biotherapeutic agent, considering its extensive antibacterial activity and robust safety profile.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"3 ","pages":"Article 100034"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The protective potential of Blighia sapida on the behavioural and hematobiochemical disruption in kerosene-exposed Clarias gariepinus: In Vivo and In Silico evaluation","authors":"M.B. Adekola ,&nbsp;O.C. Ojelade ,&nbsp;S.A. Olurode ,&nbsp;T.O. Adebowale ,&nbsp;A.O. Akinde ,&nbsp;J.T. Apata ,&nbsp;A.F. Adesina ,&nbsp;P. Oyeyemi ,&nbsp;C.U. Alichi ,&nbsp;O.T. Ajala","doi":"10.1016/j.prerep.2025.100036","DOIUrl":"10.1016/j.prerep.2025.100036","url":null,"abstract":"<div><h3>Introduction</h3><div>The discharge of petroleum products poses significant toxicological risks to aquatic life and the environment, undermining Sustainable Development Goals (SDG 14). Members of the Sapindaceae family, to which <em>Blighia sapida</em> belongs, have been utilized for the treatment of blood, heart, urinary and mental disorders. The study investigated the protective effects of ethanol extract (EE) from <em>Blighia sapida</em> stem-bark on <em>Clarias gariepinus</em> sub-adults using their behavioral, biochemical, hematological parameters, as well as <em>in silico</em> evaluation.</div></div><div><h3>Methods</h3><div>The study randomly divided 189 <em>Clarias gariepinus</em> into nine groups (G) in triplicates with seven fish to make 27 treatment tanks. The groups were G1: control, G2: 100 mL kerosene, G3: 200 mL kerosene, G4: 100 mL kerosene + 50 mg/kg bwt.EE, G5: 100 mL kerosene + 100 mg/kg bwt.EE, G6: 100 mL kerosene + 150 mg/kg bwt.EE, G7: 200 mL kerosene + 50 mg/kg bwt.EE, G8: 200 mL kerosene + 100 mg/kg bwt.EE, G9: 200 mL kerosene + 150 mg/kg bwt.EE. The experimental setup was repeated every other day for 28 days in a 30 L plastic aquaria. Behavioral parameters such as active swimming, aggression, jumping, air gulping, slothful movement, and feed response were recorded before and during the treatment using the focal sampling technique. Gas chromatography- mass spectrometry (GC-MS) of the ethanol extract was analyzed. <em>In silico</em> molecular docking studies were used to analyze the binding interactions with CYP₄₅₀ 2E1.</div></div><div><h3>Results</h3><div>The observed behavioral alterations were normalized in a dose-dependent manner at the end of the experiment, the extract mitigated kerosene-induced biochemical disruptions, reducing ALT, AST, and creatinine levels. The levels of disruptions in hematology at 100- and 200-mL kerosene exposure was restored close to control values. However, the EE displayed amelioration on the effects of kerosene. <em>In silico</em> analysis revealed high negative binding interactions between compounds identified in the extract and CYP 450 2E1. Better docking scores further validate the protective potential of the top three compounds [9-Octadecenoic acid (-7.9 kCal/mol.), (<em>E</em>)-, Hexadecanamide (-7.5 kCal/mol.), 9-Octadecenamide, (<em>Z</em>)- (-7.8 kCal/mol.)] identified in <em>B. sapida</em> ethanol extract against the molecular protein target compared with ademethionine, the standard drug (-6.6 kCal/mol.). The study concluded that <em>Blighia sapida</em> extract shows promises as a sustainable intervention against kerosene-induced toxicity in aquatic organisms. Also, the top three compounds fingerprinted from the extract of this plant, unlike the standard compound, exhibited adequate drug-like properties.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"3 ","pages":"Article 100036"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design, and evaluation of in-silico anti-cancer activity of novel gefitinib analogues against 21 target proteins","authors":"Supriti Khan Ushna ,&nbsp;Ananta Kumar Das","doi":"10.1016/j.prerep.2025.100030","DOIUrl":"10.1016/j.prerep.2025.100030","url":null,"abstract":"<div><div>Over time, evidence suggests that numerous drugs have been discovered, and computer modelling, or the \"In silico method\", to understand how anti-cancer proteins interacted. Gefitinib, a quinazoline, inhibits the EGFR tyrosine kinase by attaching to its ATP-binding site. In order to find out how effective new gefitinib analogues are at targeting cancer, this study used molecular docking and ADMET analysis. In this study, the 21 anti-cancer target proteins were utilized to perform docking by using autodock tools. Twenty new analogues were created using ligand-based design, and frontier molecular orbital analysis revealed that the designed molecules are more reactive and softer. The majority of the designed molecules have higher binding affinities than gefitinib, according to the docking studies. Additionally, the analysis revealed that the compounds C-4, C-5, C-7, C-9, C-11, C-12, and C-14 have superior affinity to more than two targets. Docking experiments of the mutated target proteins with designed molecules demonstrate that many target proteins exhibit binding properties comparable to those of the unmutated proteins. According to the literature review on targets, these molecules may be helpful in the treatment of many cancers, including tumour growth, chronic myelogenous leukemia, chronic lymphocytic leukemia, acute myeloid leukemia, acute lymphoid leukemia, thyroid cancers, and salivary gland cancers. It was shown by the binding affinity, drug similarity score, and other ADMET experiments that the designed molecules will have more significant anti-cancer activity.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"3 ","pages":"Article 100030"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143152931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional analysis of quinovic acid derivatives from Sarcocephalus pobeguinii as inhibitors of hepatitis C virus NS3/4A protease","authors":"Arnaud Fondjo Kouam ,&nbsp;Aristide Mfifen Munvera ,&nbsp;Jordas Casares Tchana Tchamba ,&nbsp;Elisabeth Menkem Zeuko’o ,&nbsp;Armelle Gaelle Kwesseu Fepa ,&nbsp;Brice Fredy Nemg Simo ,&nbsp;Felicité Syntia Douanla Somene ,&nbsp;Armel Jackson Seukep ,&nbsp;Pierre Mkounga ,&nbsp;Jules Clément Nguedia Assob ,&nbsp;Frédéric Nico Njayou ,&nbsp;Paul Fewou Moundipa","doi":"10.1016/j.prerep.2024.100026","DOIUrl":"10.1016/j.prerep.2024.100026","url":null,"abstract":"<div><div>This study assessed compounds from <em>Sarcocephalus pobeguinii</em> as potential inhibitors of HCV-NS3/4 A. Ten compounds isolated from <em>S. pobeguinii</em> were initially screened for their inhibitory activity against HCV-NS3/4 A through the fluorescence resonance energy transfer assay. The 50 % inhibitory concentration (IC₅₀) and the inhibition mechanism of active compounds were determined through concentration-response and enzyme-kinetics studies, respectively. The physical interactions between the enzyme and inhibitors were analyzed by thermal shift assay and surface plasmon resonance, while molecular interactions were predicted using molecular docking. The antiviral activity of the hit compounds was tested in a cell-based assay. Three inhibitors of HCV-NS3/4 A: Quinovic acid, Quinovic acid 3-O-[α-D-quinovopyranoside], and Quinovic acid 3-O-[β-D-quinovopyranoside] with IC₅₀ in the micromolar range were successfully identified. They displayed their inhibitory activity through a non-competitive inhibition mechanism and bound to the HCV-NS3/4 A protease in a real-time manner through 1:1 binding and steady-state affinity models, inducing its instability by lowering its melting temperature. The lead compounds effectively inhibited HCV replication at non-toxic concentrations. These results contribute to the valorization of <em>S. pobeguinii</em> as a potential source of efficient inhibitors to reinforce the current therapeutic arsenal for the treatment of HCV infection.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"3 ","pages":"Article 100026"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143151992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, characterization and evaluation of antioxidant and antibacterial activities of novel isatin-derived hydrazone derivatives of N-amino-11-azaartemisinin","authors":"Komal Rathi ,&nbsp;Priyanka Yadav ,&nbsp;Varun Rawat ,&nbsp;Aditi Pandey ,&nbsp;Achal Mukhija ,&nbsp;Biswajit Saha ,&nbsp;Ram Awatar Maurya ,&nbsp;Ved Prakash Verma","doi":"10.1016/j.prerep.2025.100033","DOIUrl":"10.1016/j.prerep.2025.100033","url":null,"abstract":"<div><div>The rise of antibiotic-resistant bacterial infections and the associated oxidative stress highlight the urgent need for new therapeutic agents. Artemisinin <strong>1</strong> and isatins are both pharmacologically active compounds with significant antibacterial, antimalarial, anticancer, and antioxidant properties respectively. In this study, we synthesized and biologically evaluated a series of isatin-derived hydrazones <strong>14a-z</strong> of <em>N</em>-amino-11-azaartemisinin, aiming to address gaps in the development of effective drugs for oxidative stress and bacterial infections. The antioxidant activity was assessed through the DPPH radical scavenging assay, revealing compound <strong>14a</strong> (IC₅₀ = 1.54 ± 0.53 μg mL⁻¹) as highly potent, comparable to L-ascorbic acid (IC₅₀ = 1.60 ± 0.62 μg mL⁻¹). Notably, antibacterial tests highlighted compound <strong>14o</strong> as having superior efficacy (MIC = 0.25 μg mL⁻¹) against <em>E. coli</em>, while <strong>14l</strong> exhibited excellent activity (MIC = 0.5 μg mL⁻¹) against <em>Bacillus subtilis</em>. In addition to these biological evaluations, we explored the thermodynamic properties of synthesised compounds using isothermal titration calorimetry (ITC) and studied the thermal stability and degradation patterns through thermogravimetric analysis (TGA). These findings underscore the potential of isatin-derived hydrazones as novel drug candidates for combating bacterial infections and free-radical associated conditions, contributing to the ongoing search for innovative therapies in the face of growing resistance and global health challenges.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"3 ","pages":"Article 100033"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canagliflozin attenuates haloperidol-induced motor and spatial working memory deficits in mice: Evidence of its role in the treatment of Parkinson’s disease","authors":"Emmanuel Semasa Irokosu ,&nbsp;Farouk Adedeji Oladoja ,&nbsp;Sunday O. Olayemi ,&nbsp;Ismail O. Ishola","doi":"10.1016/j.prerep.2024.100025","DOIUrl":"10.1016/j.prerep.2024.100025","url":null,"abstract":"<div><div>Canagliflozin (CANA), a sodium-glucose cotransporter-2 (SGLT-2) inhibitor, has been widely reported for its neuroprotective activity evidenced by its antioxidant and anti-inflammatory properties in brain tissue injury. Thus, it is a promising therapeutic candidate in the treatment of neurodegenerative diseases. This study examined the protective effect of CANA on haloperidol-induced Parkinsonism in mice. Forty-two male mice were divided randomly into seven groups: group 1 - normal control; vehicle (10 mL/kg, <em>p.o</em>.), group 2 - vehicle (pathological control; 10 mL/kg, per oral), groups 3–5, received CANA (25, 50, and 100 mg/kg, <em>p.o</em>., respectively), and the sixth and seventh groups received trihexyphenidyl (THP, 1 mg/kg, <em>p.o.,</em> standard drug), and CANA 100 mg/kg respectively, for 21 consecutive days. Animals in groups 2–6 were given haloperidol (1 mg/kg, <em>i.p</em>.) daily, one hour after pretreatment for 21 days. Spontaneous motor functions assessed with bar and rotarod test as well as working memory by Y-maze test. Thereafter, blood and discrete brain regions were collected for biochemical assays. CANA (25, 50, and 100 mg/kg) demonstrated significant reduction in cataleptic scores in both acute (22, 58, and 76 %, respectively) and chronic (46, 61, and 69 %, respectively) in bar tests at 120 minutes. CANA improved motor coordination through prolongation of latency to fall in the rotarod test. Similarly, haloperidol-induced working memory impairment was reversed by CANA evidenced in significant increase percent alternation behaviour and counteracted dopamine depletion by 57 and 68 % at 50 and 100 mg/kg, respectively. Furthermore, CANA significantly attenuated haloperidol-induced oxidative stress and TNF-α induction. It is of note that CANA did not induce hypoglycaemia in any of the treatment group. Findings from our study demonstrated the ability of canagliflozin to control muscle spasticity/weakness and working memory impairment in Parkinsonism through attenuation of oxidative stress and neuroinflammation.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"3 ","pages":"Article 100025"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143152926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin mitigates dichlorvos-initiated multi-organ dysfunction in rats by attenuating systemic oxidative stress and brain inflammatory mRNA transcripts","authors":"Adio Jamiu Akamo ,&nbsp;Boluwatife Adenike Olagunju ,&nbsp;Ofem Effiom Eteng ,&nbsp;Iyabode Adekemi Kehinde ,&nbsp;Adetutu Omolola Ojelabi ,&nbsp;Mushafau Adewale Akinsanya ,&nbsp;Adedayo Adebisi Adebisi ,&nbsp;Tobi Stephen Adekunbi ,&nbsp;Abiola Fatimoh Adenowo ,&nbsp;Flourence Anifowose ,&nbsp;Olufemi Mulkah Ajagun-Ogunleye ,&nbsp;Jacob Kehinde Akintunde","doi":"10.1016/j.prerep.2025.100029","DOIUrl":"10.1016/j.prerep.2025.100029","url":null,"abstract":"<div><div>Organophosphorus pesticides like dichlorvos (DDVP) are widely used for pest control, however their safety is a growing concern due to potential multi-factorial health risks. While curcumin (CUR) is known for its antioxidant capacity, its influence on DDVP-incited multi-organ dysfunction and brain inflammation remain under-researched. This work assessed the ameliorative competence of CUR in a DDVP-mediated systemic intoxication rat model. Rats (42) were randomly appropriated into seven groups (6 rats/group): Control, DDVP only (20 mg.kg⁻¹day⁻¹), DDVP subjected with either CUR (50 and 100 mg.kg⁻¹day⁻¹) or the reference drug atropine (0.2 mg.kg⁻¹day⁻¹), and CUR only (50 and 10 mg.kg⁻¹day⁻¹) were investigated. Rats were afflicted with DDVP orally for seven days, followed by fourteen days of CUR intervention. We humanely killed the rats and harvested their blood and viscera (liver, kidney, heart, lung, and brain) for bioassays twenty-four hours following the final treatment. CUR significantly (p &lt; 0.05) abrogated DDVP-engendered elevations in H₂O₂, NO, and malondialdehyde contents, as well as GST activity; and reversed DDVP-elicited declines in GSH amounts, activities of SOD, catalase, and glutathione peroxidase in across all tissues. In the brain, CUR markedly (p &lt; 0.05) rescinded DDVP-occasioned upregulation of gene expression biomarkers for fibrosis (TGF-β-1), apoptosis regulator (nuclear factor-kB-p65), and pro-apoptosis (p53 and Bax); and markedly abated DDVP-provoked repression of anti-inflammatory cytokine (interleukin-10)]. Wholly CUR therapy mitigated DDVP-engendered multi-organ impairments in rats via rescinding oxidative stress and brain inflammation.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"3 ","pages":"Article 100029"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143152929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"S-adenosyl-L-methionine modulates hippocampal DNA methylation, exerting fast and long-lasting anti-stress effects independent of alterations in TrkB-mTOR expression","authors":"Amanda J. Sales,&nbsp;Izaque S. Maciel,&nbsp;Francisco S. Guimarães","doi":"10.1016/j.prerep.2025.100032","DOIUrl":"10.1016/j.prerep.2025.100032","url":null,"abstract":"<div><div>Emerging evidence suggests that targeting DNA methylation, a key epigenetic mechanism, may represent a promising therapeutic approach for the treatment of stress-related disorders, including depression. Preclinical studies indicate that the inhibition of DNA methylation induces rapid and sustained antidepressant-like effects through the increased BDNF-TrkB-mTOR signaling in the prefrontal cortex, a brain region associated to the neurobiology of depression and neuroplasticity. S-adenosyl-l-methionine (SAMe), a methyl donor, has been shown to reverse depressive-like behaviors by modulating DNA methylation. However, the molecular mechanisms underlying the SAMe antidepressant effects are not yet fully understood. Therefore, this study evaluated whether SAMe induces rapid and long-lasting behavioral effects by modulating DNA methylation and mTOR-TrkB expression in mice. For this purpose, male Swiss mice received intraperitoneal (i.p.) injections of SAMe (25, 50, and 100 mg/kg) or vehicle (10 mL/kg), 30 minutes, 7 days, or 14 days prior to the forced swimming test (FST) or tail suspension test (TST), two predictive tests of antidepressant action. Global DNA methylation and mTOR-TrkB levels were measured in the hippocampus and prefrontal cortex. SAMe (50 mg/kg) induced a rapid and sustained decrease in immobility time in both the FST and TST, and prevented the stress-induced effects in hippocampal DNA methylation without changing the analyzed protein expression. These findings suggest that the rapid and sustained anti-stress effects observed in mice, resulting from the modulation of hippocampal DNA methylation due to acute SAMe treatment, are not related with TrkB-mTOR signaling.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"3 ","pages":"Article 100032"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143152930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D3 supplementation improve sexual behaviors of male wistar rats treated with flunitrazepam","authors":"David Tolulope Oluwole ,&nbsp;Oladipupo Samuel Ebiwonjumi ,&nbsp;Lydia Oluwatoyin Ajayi ,&nbsp;Ayodeji Folorunsho Ajayi","doi":"10.1016/j.prerep.2025.100028","DOIUrl":"10.1016/j.prerep.2025.100028","url":null,"abstract":"<div><div>Sexual function, a major component of male health and lifestyle, has been reported to be impaired by flunitrazepam abuse due to its central tranquilizing actions. Therefore, this study examined the role of vitamin D3 (Vit.D3) supplementation on the sexual behavior of flunitrazepam (Flu)-treated male rats. Thirty male Wistar rats, weighing 180–200 g, were allowed access to receptive female rats on three different occasions and screened for sexual proficiency; twenty male rats were selected and randomly allocated into four groups: Vehicle: receiving 0.5 ml of distilled water with 0.5 ml of olive oil;. Flu: receiving 0.35 mg/kg body weight (BW) of flunitrazepam in 0.5 ml of distilled water with 0.5 ml of olive oil; Vit. D3: receiving 0.1 μg/kg/day of vitamin D3 in 0.5 ml of olive oil with 0.5 ml distilled water; Flu + Vit. D3: receiving 0.35 mg/kg of flunitrazepam in 0.5 ml of distilled water with 0.1 μg/kg/day of Vitamin.D3 in 0.5 ml of olive oil. Treatments were administered orally for 56 days. Vitamin D3 supplementation significantly (p &lt; 0.05) reduced mount and intromission latencies, mount frequency and post-ejaculatory interval while increasing intromission frequencies, copulatory and inter-copulatory efficiencies with extended ejaculation latency in flunitrazepam-treated rats. Additionally, Vit.D3 supplementation significantly (p &lt; 0.05) increased serum concentrations of gonadotropin hormones, testosterone, and estradiol, while reducing prolactin concentration. Vitamin D3 supplementation optimized sexual performance and motivation by reducing mount and intromission latencies, improving sexual performance, and preventing premature ejaculation, thereby enhancing copulatory and inter-copulatory efficiencies. Vitamin D3 possibly optimized sexual performance by inhibiting prolactin sretion, which upregulates circulatory pituitary steroids and testicular androgens.</div></div>","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"3 ","pages":"Article 100028"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143152932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Oxidative imbalance induced by dithiocarbamate fungicide, Mancozeb aborts Nrf2 anti-oxidant signaling pathway protection in Vero cell line” [Pharmacol. Res. – Rep., Vol. 3 (2025) 100041]","authors":"Shilpa T,&nbsp;Vaishnavi A,&nbsp;Aswati Ravindranathan Nair","doi":"10.1016/j.prerep.2025.100044","DOIUrl":"10.1016/j.prerep.2025.100044","url":null,"abstract":"","PeriodicalId":101015,"journal":{"name":"Pharmacological Research - Reports","volume":"3 ","pages":"Article 100044"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}