Mechanobiology in Medicine最新文献_第7页

In silico study unfolds inhibitory potential of epicatechin gallate against SARS-CoV-2 entry and replication within the host cell 计算机研究揭示了表儿茶素没食子酸酯抑制SARS-CoV-2进入和在宿主细胞内复制的潜力

Mechanobiology in Medicine Pub Date : 2023-08-09 DOI: 10.1016/j.mbm.2023.100015

Prem Rajak, Abhratanu Ganguly

{"title":"In silico study unfolds inhibitory potential of epicatechin gallate against SARS-CoV-2 entry and replication within the host cell","authors":"Prem Rajak, Abhratanu Ganguly","doi":"10.1016/j.mbm.2023.100015","DOIUrl":"https://doi.org/10.1016/j.mbm.2023.100015","url":null,"abstract":"<div><p>Coronavirus disease-19 (COVID-19) is the ongoing pandemic affecting millions of people worldwide. Several vaccine candidates have been designed and developed for the causative virus, SARS-CoV-2. However high mutation rate in the viral genome and the emergence of new variants have challenged the effectiveness of these vaccines developed for previous strains. Hence, screening and identification of anti-SARS-CoV-2 agents having multi-target potency would be more impactful in the prevention of the disease. Epicatechin gallate (ECG) is a green tea polyphenol having various medicinal properties, including anti-oxidative and anti-inflammatory effects. However its role as anti-SARS-CoV-2 agent is not clear. Hence the present in silico study aims to investigate the binding potential of ECG with several proteins which are critical to SARS-CoV-2 entry and replication within the host cell. Molecular docking analyses have revealed that ECG could potentially block several amino acid residues of entry factors in host cells, spike protein, and many non-structural proteins through Hydrogen bonds and hydrophobic interactions. Such interactions with vital proteins could inhibit SARS-CoV-2 entry and its subsequent replication into the host. Therefore, ECG could be a potential therapeutic agent for the prevention of COVID-19. However, the findings of the present study demand further validation in animal models.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"1 2","pages":"Article 100015"},"PeriodicalIF":0.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49906650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Distribution of intracellular calcium during flow-induced migration of RAW264.7 cells RAW264.7 细胞在流动诱导迁移过程中的细胞内钙分布

Mechanobiology in Medicine Pub Date : 2023-08-09 DOI: 10.1016/j.mbm.2023.100012

Shurong Wang , Qing Sun , Yang Zhao , Bo Huo

{"title":"Distribution of intracellular calcium during flow-induced migration of RAW264.7 cells","authors":"Shurong Wang , Qing Sun , Yang Zhao , Bo Huo","doi":"10.1016/j.mbm.2023.100012","DOIUrl":"10.1016/j.mbm.2023.100012","url":null,"abstract":"<div><p>Cell migration is an important biological process regulated by mechanical stimulation, which leads to intracellular calcium response. Cell migration are dependent on the distribution and dynamic changes of intracellular calcium concentration. However, the temporal relation among mechanical stimulation, cell migration, and intracellular calcium distribution remains unclear. In this study, unidirectional flow and oscillatory flow were applied on osteoclast precursor RAW264.7 cells. The parameters of cell migration under fluid flow and intracellular calcium distribution along the migration or flow direction were calculated. Experimental results suggest the cells to adjust the [Ca<sup>2+</sup>]<sub>i</sub> distribution in the migration direction is independent of flow application or the reverse of flow direction, but the [Ca<sup>2+</sup>]<sub>i</sub> distribution in the flow direction is determined by the [Ca<sup>2+</sup>]<sub>i</sub> distribution-adjusting ability of cells and flow stimulation. Blocking calcium signaling pathways, namely, mechanosensitive cation-selective channels, phospholipase C, and endoplasmic reticulum, and removing extracellular calcium inhibited cell migration along the flow direction and the gradient distribution of intracellular calcium. This study provided insights into the mechanism of flow-induced cell migration and quantitative data for the recruitment of osteoclast precursors targeting the location of bone resorption.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"2 1","pages":"Article 100012"},"PeriodicalIF":0.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907023000128/pdfft?md5=7b57f22e0cac83ae0794c8da4fd1ba1d&pid=1-s2.0-S2949907023000128-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78454389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Matrix stiffness-driven cancer progression and the targeted therapeutic strategy 基质刚度驱动的癌症进展和靶向治疗策略

Mechanobiology in Medicine Pub Date : 2023-08-03 DOI: 10.1016/j.mbm.2023.100013

Rui Liang, Guanbin Song

引用次数: 0

Tumor state transitions driven by Gaussian and non-Gaussian noises 由高斯和非高斯噪声驱动的肿瘤状态转换

Mechanobiology in Medicine Pub Date : 2023-07-20 DOI: 10.1016/j.mbm.2023.100011

Mengjiao Hua , Yu Wu

{"title":"Tumor state transitions driven by Gaussian and non-Gaussian noises","authors":"Mengjiao Hua , Yu Wu","doi":"10.1016/j.mbm.2023.100011","DOIUrl":"https://doi.org/10.1016/j.mbm.2023.100011","url":null,"abstract":"<div><p>Tumor state transitions between the excited (high-concentration) and nonexcited (low-concentration) basins under the Gaussian white noise and non-Gaussian colored noise are investigated via the most probable steady states (MPSS) and the first escape probability (FEP)-based stochastic basin of attraction (SBA), respectively. Reducing the non-Gaussian colored noise and then utilizing the unified colored noise approximation (UCNA), the Markov system is derived. The extremal controlling equation of stationary probability density function (SPDF) is derived to analyze the impacts of noise on transitions in terms of MPSS. The existence of the ‘color’ of the non-Gaussian colored noise induces the reappearance of the uncorrelated additive white noise parameter that had vanished from the extremal controlling equation, completely reversing the inability of the uncorrelated additive Gaussian white noise to operate on transitions. The FEP-dependent SBA characterizing the excited basin stability is performed to further analyze the role of noise on the likelihood of escaping to the nonexcited state. Results show that the cross-correlated noises play a dual role in regulating SBA. The increased SBA indicating more difficulty to escape to the nonexcited state reflects a worse therapeutic effect. Therefore, enhancing the negatively correlated noise intensities and augmenting the non-Gaussian noise correlation time is essential for destabilizing the excited basin and achieving optimal therapeutic efficacy.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"1 2","pages":"Article 100011"},"PeriodicalIF":0.0,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49906652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanobiology: Methods and Protocols 机械生物学:方法和方案

Mechanobiology in Medicine Pub Date : 2023-01-01 DOI: 10.1007/978-1-0716-2851-5

引用次数: 0

Copyright 版权

Mechanobiology in Medicine Pub Date : 2020-01-01 DOI: 10.1016/b978-0-12-817931-4.12001-1

引用次数: 0

Dedication 奉献

Mechanobiology in Medicine Pub Date : 2020-01-01 DOI: 10.1016/b978-0-12-817931-4.03001-6

引用次数: 0

Glossary 术语表

Mechanobiology in Medicine Pub Date : 2020-01-01 DOI: 10.1016/b978-0-12-817931-4.17001-3

引用次数: 0

Front matter 前页

Mechanobiology in Medicine Pub Date : 2020-01-01 DOI: 10.1016/b978-0-12-817931-4.01001-3

引用次数: 0

Index 指数

Mechanobiology in Medicine Pub Date : 2020-01-01 DOI: 10.1016/b978-0-12-817931-4.20001-0

引用次数: 0