Mechanobiology in Medicine最新文献_第7页

Changes of calcium cycling in HFrEF and HFpEF HFrEF和HFpEF中钙循环的变化

Mechanobiology in Medicine Pub Date : 2023-09-01 DOI: 10.1016/j.mbm.2023.100001

Jian Shou , Yunlong Huo

引用次数: 0

The application of ECM-derived biomaterials in cartilage tissue engineering 细胞外基质生物材料在软骨组织工程中的应用

Mechanobiology in Medicine Pub Date : 2023-09-01 DOI: 10.1016/j.mbm.2023.100007

Yu-wei Wang , Ming-ze Du , Tuo Wu , Tong Su , Li-ya Ai , Dong Jiang

引用次数: 0

ChatGPT for mechanobiology and medicine: A perspective 机械生物学和医学的ChatGPT:一个展望

Mechanobiology in Medicine Pub Date : 2023-09-01 DOI: 10.1016/j.mbm.2023.100005

Minyu Chen, Guoqiang Li

引用次数: 0

Mechanobiology in cellular, molecular, and tissue adaptation 细胞、分子和组织适应的机械生物学

Mechanobiology in Medicine Pub Date : 2023-08-24 DOI: 10.1016/j.mbm.2023.100022

Yi-Xian Qin, Jie Zhao

{"title":"Mechanobiology in cellular, molecular, and tissue adaptation","authors":"Yi-Xian Qin, Jie Zhao","doi":"10.1016/j.mbm.2023.100022","DOIUrl":"https://doi.org/10.1016/j.mbm.2023.100022","url":null,"abstract":"<div><p>The use of mechanical biology and biomechanical signal transduction is a novel approach to attenuate biological tissue degeneration, whereas the understanding of specific cellular responses is critical to delineate the underlying mechanism. Dynamic mechanical signals with optimized loading signals, i.e., intensity and frequency, have been shown to have the potential to regulate adaptation and regeneration. Mechanotransduction pathways are of great interest in elucidating how mechanical signals produce such observed effects, including reduced tissue mass loss, increased healing and formation, and cell differentiation. While mechanobiology in the adaptation of cells and tissues is observed and recorded in the literature, its application in disease mechanism and treatment is under development. We would congratulate the opening of the Mechanobiology in Medicine journal, which provides an effective platform for advanced research in basic mechanotransduction and its translation in disease diagnosis, therapeutics, and beyond. This review aims to develop a cellular and molecular understanding of the mechanotransduction processes in tissue regeneration, which may provide new insights into disease mechanisms and the promotion of healing. Particular attention is allotted to the responses of mechanical loading, including potential cellular and molecular pathways, such as mechanotransduction associated with mechanotransduction pathways (e.g., Piezo ion channels and Wnt signaling), immune-response, neuron development, tissue adaptation and repair, and stem cell differentiation. Altogether, these discussed data highlight the complex yet highly coordinated mechanotransduction process in tissue regeneration.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"1 2","pages":"Article 100022"},"PeriodicalIF":0.0,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49906639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Extracellular cell matrix stiffness-driven drug resistance of breast cancer cells via EGFR activation 细胞外细胞基质刚度通过EGFR激活驱动乳腺癌细胞耐药

Mechanobiology in Medicine Pub Date : 2023-08-22 DOI: 10.1016/j.mbm.2023.100023

Tingting Li , Yichao Li , Hao Wu , Chong Peng , Jiawen Wang , Shihuan Chen , Tian Zhao , Shun Li , Xiang Qin , Yiyao Liu

{"title":"Extracellular cell matrix stiffness-driven drug resistance of breast cancer cells via EGFR activation","authors":"Tingting Li , Yichao Li , Hao Wu , Chong Peng , Jiawen Wang , Shihuan Chen , Tian Zhao , Shun Li , Xiang Qin , Yiyao Liu","doi":"10.1016/j.mbm.2023.100023","DOIUrl":"https://doi.org/10.1016/j.mbm.2023.100023","url":null,"abstract":"<div><p>Tumor progression is accompanied by complex structural changes in the extracellular matrix (ECM), which decrease the effective exposure of tumors to drugs. Breast cancer are highly heterogeneous with a typically high degree of ECM remodeling and stiffening. Therefore, it is especially important to explore the influence of ECM stiffness on breast cancer chemotherapy. Here, we fabricated 3D Methacrylate Gelatin (GelMA) hydrogels with varying stiffness by photo-crosslinking to simulate the change of tissue stiffness during the development of breast cancer. These 3D hydrogels were used to evaluate how MDA-MB-231 cells responded to the chemotherapy drug doxorubicin (DOX), the mechanical regulatory mechanism involved has also been investigated. The findings demonstrated that 15% GelMA hydrogel (9 kPa) increased the activity of EGFR to block the Hippo signaling pathway and activate Yes-associated protein (YAP). Activated YAP allowed cytosolic EGFR transport into the nucleus via binding with it, up-regulated the expression of their respective transcriptional targets, and thus generates drug resistance. Altogether, our study implicates that stiffness-dependent EGFR activation plays an important role in breast cancer drug resistance, indicating that targeting of both YAP and EGFR signals may present a promising therapeutic strategy for ECM stiffness-induced drug resistance.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"1 2","pages":"Article 100023"},"PeriodicalIF":0.0,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49906638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Patient-derived tumor models and their distinctive applications in personalized drug therapy 患者来源的肿瘤模型及其在个性化药物治疗中的独特应用

Mechanobiology in Medicine Pub Date : 2023-08-09 DOI: 10.1016/j.mbm.2023.100014

Jia He , Chunhe Zhang , Alican Ozkan , Tang Feng , Peiyan Duan , Shuo Wang , Xinrui Yang , Jing Xie , Xiaoheng Liu

{"title":"Patient-derived tumor models and their distinctive applications in personalized drug therapy","authors":"Jia He , Chunhe Zhang , Alican Ozkan , Tang Feng , Peiyan Duan , Shuo Wang , Xinrui Yang , Jing Xie , Xiaoheng Liu","doi":"10.1016/j.mbm.2023.100014","DOIUrl":"https://doi.org/10.1016/j.mbm.2023.100014","url":null,"abstract":"<div><p>Tumor models <em>in vitro</em> are conventional methods for developing anti-cancer drugs, evaluating drug delivery, or calculating drug efficacy. However, traditional cell line-derived tumor models are unable to capture the tumor heterogeneity in patients or mimic the interaction between tumors and their surroundings. Recently emerging patient-derived preclinical cancer models, including of patient-derived xenograft (PDX) model, circulating tumor cell (CTC)-derived model, and tumor organoids-on-chips, are promising in personalized drug therapy by recapitulating the complexities and personalities of tumors and surroundings. These patient-derived models have demonstrated potential advantages in satisfying the rigorous demands of specificity, accuracy, and efficiency necessary for personalized drug therapy. However, the selection of suitable models is depending on the specific therapeutic requirements dictated by cancer types, progressions, or the assay scale. As an example, PDX models show remarkable advantages to reconstruct solid tumors in vitro to understand drug delivery and metabolism. Similarly, CTC-derived models provide a sensitive platform for drug testing in advanced-stage patients, while also facilitating the development of drugs aimed at suppressing tumor metastasis. Meanwhile, the demand for large-scale testing has promoted the development of tumor organoids-on-chips, which serves as an optimal tool for high-throughput drug screening. This review summarizes the establishment and development of PDX, CTC-derived models, and tumor organoids-on-chips and addresses their distinctive advantages in drug discovery, sensitive testing, and screening, which demonstrate the potential to aid in the selection of suitable models for fundamental cancer research and clinical trials, and further developing the personalized drug therapy.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"1 2","pages":"Article 100014"},"PeriodicalIF":0.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49906649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico study unfolds inhibitory potential of epicatechin gallate against SARS-CoV-2 entry and replication within the host cell 计算机研究揭示了表儿茶素没食子酸酯抑制SARS-CoV-2进入和在宿主细胞内复制的潜力

Mechanobiology in Medicine Pub Date : 2023-08-09 DOI: 10.1016/j.mbm.2023.100015

Prem Rajak, Abhratanu Ganguly

{"title":"In silico study unfolds inhibitory potential of epicatechin gallate against SARS-CoV-2 entry and replication within the host cell","authors":"Prem Rajak, Abhratanu Ganguly","doi":"10.1016/j.mbm.2023.100015","DOIUrl":"https://doi.org/10.1016/j.mbm.2023.100015","url":null,"abstract":"<div><p>Coronavirus disease-19 (COVID-19) is the ongoing pandemic affecting millions of people worldwide. Several vaccine candidates have been designed and developed for the causative virus, SARS-CoV-2. However high mutation rate in the viral genome and the emergence of new variants have challenged the effectiveness of these vaccines developed for previous strains. Hence, screening and identification of anti-SARS-CoV-2 agents having multi-target potency would be more impactful in the prevention of the disease. Epicatechin gallate (ECG) is a green tea polyphenol having various medicinal properties, including anti-oxidative and anti-inflammatory effects. However its role as anti-SARS-CoV-2 agent is not clear. Hence the present in silico study aims to investigate the binding potential of ECG with several proteins which are critical to SARS-CoV-2 entry and replication within the host cell. Molecular docking analyses have revealed that ECG could potentially block several amino acid residues of entry factors in host cells, spike protein, and many non-structural proteins through Hydrogen bonds and hydrophobic interactions. Such interactions with vital proteins could inhibit SARS-CoV-2 entry and its subsequent replication into the host. Therefore, ECG could be a potential therapeutic agent for the prevention of COVID-19. However, the findings of the present study demand further validation in animal models.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"1 2","pages":"Article 100015"},"PeriodicalIF":0.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49906650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Distribution of intracellular calcium during flow-induced migration of RAW264.7 cells RAW264.7 细胞在流动诱导迁移过程中的细胞内钙分布

Mechanobiology in Medicine Pub Date : 2023-08-09 DOI: 10.1016/j.mbm.2023.100012

Shurong Wang , Qing Sun , Yang Zhao , Bo Huo

{"title":"Distribution of intracellular calcium during flow-induced migration of RAW264.7 cells","authors":"Shurong Wang , Qing Sun , Yang Zhao , Bo Huo","doi":"10.1016/j.mbm.2023.100012","DOIUrl":"10.1016/j.mbm.2023.100012","url":null,"abstract":"<div><p>Cell migration is an important biological process regulated by mechanical stimulation, which leads to intracellular calcium response. Cell migration are dependent on the distribution and dynamic changes of intracellular calcium concentration. However, the temporal relation among mechanical stimulation, cell migration, and intracellular calcium distribution remains unclear. In this study, unidirectional flow and oscillatory flow were applied on osteoclast precursor RAW264.7 cells. The parameters of cell migration under fluid flow and intracellular calcium distribution along the migration or flow direction were calculated. Experimental results suggest the cells to adjust the [Ca<sup>2+</sup>]<sub>i</sub> distribution in the migration direction is independent of flow application or the reverse of flow direction, but the [Ca<sup>2+</sup>]<sub>i</sub> distribution in the flow direction is determined by the [Ca<sup>2+</sup>]<sub>i</sub> distribution-adjusting ability of cells and flow stimulation. Blocking calcium signaling pathways, namely, mechanosensitive cation-selective channels, phospholipase C, and endoplasmic reticulum, and removing extracellular calcium inhibited cell migration along the flow direction and the gradient distribution of intracellular calcium. This study provided insights into the mechanism of flow-induced cell migration and quantitative data for the recruitment of osteoclast precursors targeting the location of bone resorption.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"2 1","pages":"Article 100012"},"PeriodicalIF":0.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907023000128/pdfft?md5=7b57f22e0cac83ae0794c8da4fd1ba1d&pid=1-s2.0-S2949907023000128-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78454389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Matrix stiffness-driven cancer progression and the targeted therapeutic strategy 基质刚度驱动的癌症进展和靶向治疗策略

Mechanobiology in Medicine Pub Date : 2023-08-03 DOI: 10.1016/j.mbm.2023.100013

Rui Liang, Guanbin Song

引用次数: 0

Tumor state transitions driven by Gaussian and non-Gaussian noises 由高斯和非高斯噪声驱动的肿瘤状态转换

Mechanobiology in Medicine Pub Date : 2023-07-20 DOI: 10.1016/j.mbm.2023.100011

Mengjiao Hua , Yu Wu

{"title":"Tumor state transitions driven by Gaussian and non-Gaussian noises","authors":"Mengjiao Hua , Yu Wu","doi":"10.1016/j.mbm.2023.100011","DOIUrl":"https://doi.org/10.1016/j.mbm.2023.100011","url":null,"abstract":"<div><p>Tumor state transitions between the excited (high-concentration) and nonexcited (low-concentration) basins under the Gaussian white noise and non-Gaussian colored noise are investigated via the most probable steady states (MPSS) and the first escape probability (FEP)-based stochastic basin of attraction (SBA), respectively. Reducing the non-Gaussian colored noise and then utilizing the unified colored noise approximation (UCNA), the Markov system is derived. The extremal controlling equation of stationary probability density function (SPDF) is derived to analyze the impacts of noise on transitions in terms of MPSS. The existence of the ‘color’ of the non-Gaussian colored noise induces the reappearance of the uncorrelated additive white noise parameter that had vanished from the extremal controlling equation, completely reversing the inability of the uncorrelated additive Gaussian white noise to operate on transitions. The FEP-dependent SBA characterizing the excited basin stability is performed to further analyze the role of noise on the likelihood of escaping to the nonexcited state. Results show that the cross-correlated noises play a dual role in regulating SBA. The increased SBA indicating more difficulty to escape to the nonexcited state reflects a worse therapeutic effect. Therefore, enhancing the negatively correlated noise intensities and augmenting the non-Gaussian noise correlation time is essential for destabilizing the excited basin and achieving optimal therapeutic efficacy.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"1 2","pages":"Article 100011"},"PeriodicalIF":0.0,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49906652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0