Journal of Cellular Immunotherapy最新文献

{"title":"Biological activity of LALF32-51–E7, a vaccine candidate for the treatment of anogenital lesions associated to HPV16","authors":"Alain B. Alfonso ,&nbsp;Milaid Granadillo ,&nbsp;Aileen Batte ,&nbsp;Enma Brown ,&nbsp;Miladys Limonta ,&nbsp;Dayana Soler ,&nbsp;Dinorah Torres ,&nbsp;Yayrí C. Prieto ,&nbsp;Laura Varas ,&nbsp;Isis Torrens","doi":"10.1016/j.jocit.2018.07.001","DOIUrl":"10.1016/j.jocit.2018.07.001","url":null,"abstract":"<div><p>Cervical cancer is the second leading cause of cancer death in women. It is well established that human papillomavirus (HPV) is the cause of virtually 100% of these cancers, 70% of which are attributable to infections with HPV types 16 or 18. We developed a fusion protein comprising a cell penetrating and immunostimulatory peptide corresponding to residues 32 to 51 of the <em>Limulus polyphemus</em> protein (LALF32-51) linked to the HPV16 E7 antigen (LALF_32-51–E7) that is obtained from <em>Escherichia coli</em>. The development of new treatments as mentioned requires a robust and reproducible procedure for batch release. We used the combination of three batches of LALF_32-51-E7 with Al(OH)₃ in an experimental design to develop a biological activity test for batch release of this candidate, which uses prophylactic immunization in the TC-1 tumor mouse model. Two injections, spaced for 7 days with LALF_31-52-E7+Al(OH)₃ are sufficient for inducing a potent anti-tumor response in the TC-1 tumor model. LALF_31-52-E7 vaccine candidates in combination with Al(OH)₃, exhibited in C57BL/6 mice over 86% protection against tumor challenge and high titers of specific antibodies against LALF_31-52-E7. The results obtained across three batches of LALF_32-51–E7+Al(OH)₃ with this simple and uncomplicated assay based on prophylactic immunization in the TC-1 mouse model suggest that it would be feasible to implement it as a biological activity assay for the release of production batches of LALF_32-51–E7.</p></div>","PeriodicalId":100761,"journal":{"name":"Journal of Cellular Immunotherapy","volume":"4 2","pages":"Pages 71-74"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jocit.2018.07.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88723733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutrophil secretion modulates neutrophil and monocyte functions during hyperglucose and/or hyperinsulin conditions in vitro","authors":"P. Neethi Raj,&nbsp;Binchu V. Shaji,&nbsp;V.H. Haritha,&nbsp;Y. Anie","doi":"10.1016/j.jocit.2018.02.001","DOIUrl":"10.1016/j.jocit.2018.02.001","url":null,"abstract":"<div><p>Neutrophils, the first cells that come to the infection site, regulate the recruitment and functioning of monocytes. Alteration in neutrophil secretions during pathological conditions result in altered neutrophil and monocyte functions. As hyperglucose and hyperisulin conditions are known to prime cells of innate immune system, the role of neutrophil secretion in activating or priming neutrophils and monocytes during these conditions was studied.</p><p>Neutrophils were isolated and treated with hyperglucose, hyperinsulin or hyperglucose + hyperinsulin conditions <em>in vitro</em> and the neutrophil supernatants were collected. Neutrophil and monocyte cells treated with these supernatants were then studied for their functions such as chemotaxis, phagocytosis, NBT reduction and killing ability.</p><p>Secretions of unstimulated neutrophils suppressed chemotaxis, phagocytosis and killing ability of monocytes but it did not affect the functions of neutrophils. Hyperinsulin and hyperglucose + hyperinsulin-induced neutrophil secretions caused reduction in phagocytic index of neutrophils without affecting other functions. Neutrophil secretions collected during hyperglucose or hyperinsulin did not alter monocyte functions significantly when compared to secretion collected from unstimulated neutrophils. In conclusion, the study reveals the role of neutrophil secretions in modulating neutrophil and monocyte functions in hyperglucose and hyperinsulin conditions.</p></div>","PeriodicalId":100761,"journal":{"name":"Journal of Cellular Immunotherapy","volume":"4 2","pages":"Pages 65-70"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jocit.2018.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75107573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of HTTLPR gene polymorphism fluctuation in type II diabetes","authors":"Negin Jafarian ,&nbsp;Mohammad Hasan Sheikhha ,&nbsp;Zahra Sahraee ,&nbsp;Ali Akbar Samadani","doi":"10.1016/j.jocit.2018.10.001","DOIUrl":"10.1016/j.jocit.2018.10.001","url":null,"abstract":"<div><h3>Background</h3><p>One of the most common public health problems is type 2 diabetes (T2DM). Several genes have been identified as remarkable risk factors for T2DM. However, it seems that the disease is caused by both genetic and environmental factors. Correspondingly, the aim of this study was to investigate the association between polymorphisms of <em>HTTLPR</em> with T2DM in Yazd city, Iran.</p></div><div><h3>Methods</h3><p>In this case-control study, blood samples of 150 patients with T2DM with or without complications were referred to the Central Laboratory in Yazd in order to determine the polymorphism of 5-<em>HTTLPR,</em> a serotonin transporter gene. The results were compared with 150 normal subjects without diabetes.</p></div><div><h3>Results</h3><p>The average age of patients and the control group were 55.91 ± 7.38 and 46.61 ± 9.15 years respectively. In the patient group the frequencies of genotypes LL, LS and SS were 34.7%, 40.7% and 24.7% respectively. Among the control group, the frequencies were 38.7%, 30%, and 31.3% respectively. There was no significant difference for the polymorphism genotype frequencies between the patients group and the control group (p = 0.140). Moreover, there was no significant relationship between genotype polymorphism of the 5-<em>HTTPLR</em> and complications of diabetes including retinopathy (p = 0.085), nephropathy (p = 0.566) and neuropathy (p = 0.039).</p></div><div><h3>Conclusion</h3><p>Based on the results of this study, there was no significant association between 5-<em>HTTLPR</em> polymorphism in a population with T2DM living in Yazd, Iran. Moreover, there was no significant relationship between age, sex and BMI of patients with frequency of allele polymorphism of 5-<em>HTTLPR</em>.</p></div>","PeriodicalId":100761,"journal":{"name":"Journal of Cellular Immunotherapy","volume":"4 2","pages":"Pages 79-82"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jocit.2018.10.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86073217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on nanotechnology based innovations in diagnosis and treatment of multiple sclerosis","authors":"Smriti Ojha ,&nbsp;Babita Kumar","doi":"10.1016/j.jocit.2017.12.001","DOIUrl":"10.1016/j.jocit.2017.12.001","url":null,"abstract":"<div><p>Multiple Sclerosis (MS) is a multifactorial disease with several physiologic and pathogenic mechanisms and pathways. In MS the immune system attacks the myelin sheath that provides insulation to the nerve cells in their brain, spinal cord and optic nerve. With the damaged and compromised nerve membrane, the nerve cells are not able to conduct electrical signals properly, resulting in limb numbness, paralysis, blindness and other neurological disorders. The main purpose of this paper is to highlight advancements in nanotechnology that has enabled the clinician to cross blood brain barrier and to target the brain and CNS of the patient with multiple sclerosis. We have focused on application of nanotechnology having therapeutic and imaging components to improve tissue imaging, targeted interactions at molecular level, nerve protection and regeneration therapy. We have also discussed the pathophysiology of the disease and nanotechnology based new strategies to deliver the therapeutic candidates for the diagnosis and treatment of MS. Future advances in the development of new practical treatment modalities for the treatment of MS is currently a potential area of research.</p></div>","PeriodicalId":100761,"journal":{"name":"Journal of Cellular Immunotherapy","volume":"4 2","pages":"Pages 56-64"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jocit.2017.12.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87496934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre skin prick testing: A curfew required on herbals too","authors":"Taha A. Qureshi ,&nbsp;Qurteeba Qadri ,&nbsp;Tabasum Shafi ,&nbsp;Zafar A. Shah ,&nbsp;Asif Kundangar ,&nbsp;Roohi Rasool","doi":"10.1016/j.jocit.2018.07.002","DOIUrl":"10.1016/j.jocit.2018.07.002","url":null,"abstract":"<div><h3>Background</h3><p>Allergic diseases are hypersensitivity disorders induced by an allergen specific immunoglobulin E (IgE) mediated response; Skin PrickTest (SPT) remains the gold standard for the in vivo assessment of IgEs. Many drugs are known to have anti histaminic effects and as such need to be stopped prior to the testing. However, the role of alternative system of medicine and other herbal remedies remains controversial in this regard.</p></div><div><h3>Objectives</h3><p>To study the effect of alternative system of medicine and other herbal remedies on the results of SPT.</p></div><div><h3>Methods</h3><p>The patients having a strong history and clinical symptoms of allergic diseases like allergic rhinitis, bronchial asthma and chronic urticaria were subjected to skin prick testing.</p></div><div><h3>Results</h3><p>There were six patients whose SPT results were negative although history and clinical symptoms suggested otherwise. On probing the drug intake of the patients prior to the testing, it was observed that the patients had taken certain herbal medications which interfered with the allergy testing and gave false negatives.</p></div><div><h3>Conclusion</h3><p>It is for the first time that a study has reported the significant effect of alternative medicine and other herbal supplements in suppression of skin prick test results. Therefore it is extremely important to ask properly the history of intake of alternative medicine and other dietary herbal ingredients and then insist on its abstinence for 10 days before SPT. A protocol wherein the allergist recommends to stop the use of any regional herbs prior to allergy testing will be the right step in improving the results of allergy testing and preventing false positives.</p></div>","PeriodicalId":100761,"journal":{"name":"Journal of Cellular Immunotherapy","volume":"4 2","pages":"Pages 83-86"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jocit.2018.07.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75840593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The miRNA binding site SNP in the 3′ UTR of NBS1 is inversely associated with breast cancer risk","authors":"Nafezeh Ghanei ,&nbsp;Kamran Ghaedi ,&nbsp;Masoumeh Mansouri bidakani ,&nbsp;Mohammad Fazilati ,&nbsp;Habibollah Nazem","doi":"10.1016/j.jocit.2018.09.013","DOIUrl":"10.1016/j.jocit.2018.09.013","url":null,"abstract":"<div><h3>Aim</h3><p>Nijmegen breakage syndrome 1 (<em>NBS1</em>), also known as <em>NBN</em>, participates in cellular response to DNA damage and maintaining genomic stability. Functional polymorphisms located at a microRNA binding site in the 3′ UTR of <em>NBS1</em> have been frequently studied in various cancers, but the results are inconsistent. The aim of the present study was to test a possible association between breast cancer risk and the <em>NBS1</em> genetic variant, <em>NBS1</em> rs2735383 541G &gt; C, by performing a case–control study in an Iranian population.</p></div><div><h3>Materials &amp; methods</h3><p>Allele-specific primer PCR method was utilized to genotype the genetic variant in 129 women with breast cancer and 117 healthy controls. The association tests with risk of breast cancer were examined by logistic regression.</p></div><div><h3>Results</h3><p>The <em>NBS1</em> rs2735383 541G &gt; C polymorphism was inversely associated with breast cancer (GC + CC vs GG, OR = 0.42, 95% C.I = 0.25–0.72, <em>P</em> value = 0.001; C vs G allele, OR = 0.43, 95% C.I = 0.29–0.62, <em>P</em> value &lt; 0.001) and also lymph node metastasis (GC + CC vs GG, OR = 0.23, 95% C.I = 0.11–0.52, <em>P</em> value &lt; 0.001).</p></div><div><h3>Conclusion</h3><p>The rs2735383 variant may be a genetic modifier for breast cancer development in Iranians.</p></div>","PeriodicalId":100761,"journal":{"name":"Journal of Cellular Immunotherapy","volume":"4 2","pages":"Pages 75-78"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jocit.2018.09.013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79287889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune checkpoint inhibition in gastric cancer: A systematic review","authors":"Adamu Ishaku Akyala ,&nbsp;Auke P. Verhaar ,&nbsp;Maikel P. Peppelenbosch","doi":"10.1016/j.jocit.2018.05.001","DOIUrl":"10.1016/j.jocit.2018.05.001","url":null,"abstract":"<div><h3>Objectives</h3><p>Checkpoint inhibitor therapy is revolutionizing the management of the oncological disease. Despite the fact that gastric cancer is a common malignancy with a relatively poor prognosis, checkpoint inhibitor therapy has attracted relatively little attention for the treatment of this disease. We decided to perform a systematic review focusing on the potential usefulness of checkpoint inhibitors in patients with gastric cancer by reviewing all clinical trials involving checkpoint inhibitors in gastric cancer up to the 9th of September 2017.</p></div><div><h3>Methods</h3><p>We systematically review all randomized controlled trials on the 9th September 2017. We searched the <span>embase.com</span><svg><path></path></svg>, Medline Ovid, Cochrane CENTRAL, Web of Science, Google Scholar, and databases of current ongoing randomized trials. A description assessment of randomized clinical trials (RCTs) was performed on Immune checkpoint inhibitors for PD1, CTLA4 and PD-L1 (Atezolizumab, Avelumab, Durvalumab, Ipilimumab, Nivolumab, Pembrolizumab, and Tremelimumab) in gastric cancer patients.</p></div><div><h3>Results</h3><p>The current body of biomedical literature describes 3621 gastric cancer patients being treated with checkpoint inhibitors and compared to patients receiving chemotherapy. PD-1 inhibitors pembrolizumab and nivolumab in combination appear to substantially outperform conventional chemotherapy, with more extended overall survival median (OS; 10.0 vs. 6.0 mo), a more significant objective response rate (36% versus 13%, p = 0.10), with a reduced adverse event rate (33% vs. 22%), although other checkpoint inhibitors appear somewhat less effective.</p></div><div><h3>Translational Impact</h3><p>It seems that checkpoint inhibitor therapy in general, and PD-1-directed therapy, in particular, constitutes a rational therapeutic avenue for advanced gastric cancer.</p></div>","PeriodicalId":100761,"journal":{"name":"Journal of Cellular Immunotherapy","volume":"4 2","pages":"Pages 49-55"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jocit.2018.05.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73018050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Theoretical numerical modeling of the oxygen diffusion effects within the periodontal ligament for orthodontic tooth displacement","authors":"Camille Spingarn ,&nbsp;Delphine Wagner ,&nbsp;Yves Rémond ,&nbsp;Daniel George","doi":"10.1016/j.jocit.2018.09.011","DOIUrl":"10.1016/j.jocit.2018.09.011","url":null,"abstract":"<div><p>Orthodontic tooth movement results from alveolar bone remodelling around the tooth. With applied mechanical forces, the periodontal ligament and its vascularization are deformed, the blood flow changes and so the amount of oxygen import, provoking, through cell density variation, bone remodelling and tooth movement. A numerical finite element (FE) model is presented in which the amount of oxygen and the cells densities of osteoclasts and osteoblasts are deduced from the oxygen variation inside the deformed periodontal ligament, leading to bone remodelling. Predicting this evolution will help the orthodontists in their decision making for patient dependent treatments.</p></div>","PeriodicalId":100761,"journal":{"name":"Journal of Cellular Immunotherapy","volume":"4 1","pages":"Pages 44-47"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jocit.2018.09.011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77557020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"G protein-coupled receptor 120 in mediating the regulation effect of saturated and unsaturated long-chain fatty acid on human adipocytes","authors":"Zhen Li ,&nbsp;Xuefeng Li ,&nbsp;Yuwen Wu ,&nbsp;Qi Huang ,&nbsp;Li Sun ,&nbsp;Jean-François Stoltz ,&nbsp;Zhe Dai","doi":"10.1016/j.jocit.2018.09.012","DOIUrl":"10.1016/j.jocit.2018.09.012","url":null,"abstract":"<div><h3>Objective</h3><p>To explore G protein-coupled receptor 120 (GPR120)'s role in mediating saturated and unsaturated long-chain fatty acid on adipocyte inflammation, ER stress and insulin signaling pathway.</p></div><div><h3>Methods</h3><p>Omental adipose tissue from 5 patients undergoing elective gallstone removal surgery was collected and the primary adipocytes were isolated and cultured. In the first experiment, cells were divided into the following four groups: (1) Control group; (2): Docosahexaenoic acid (DHA) group; (3)DHA+10 μmol/L GW9508 group; and (4) DHA+20 μmol/L GW9508 group. In the second experiment, cells were divided into the following four groups:(1) Control group; (2) Palmic acid (PA) group; (3) PA+10 μmol/L GW9508 group; and (4) PA+20 μmol/L GW9508 group. Real time PCR and western blot were used to evaluate the gene and protein expression of GPR120, FABP4, inflammatory factors and ER stress markers.</p></div><div><h3>Results</h3><p>(1) Under the stimulation of DHA and PA, the expression of GPR and FABP4 were increased. But with inhibition of GPR120, the expression of GPR120and FABP4 decreased in both DHA and PA treatment groups.(2) DHA treatment could decrease TNF-alpha expression and inflammation factor mRNA levels in primary adipocytes. Production of these factors increased with inhibition of GPR120. PA treatment could increase TNF-alpha expression and inflammation factor mRNA levels in primary adipocytes. They were further increased by inhibition of GPR120. (3) DHA treatment could decrease ER stress in primary adipocytes. GPR120 inhibition could promote ER stress. PA treatment could increase ER stress in primary adipocytes. Again, GPR120 inhibition further increased ER stress. <strong>Conclusion</strong> GPR120 mediated long-chain fatty acid-related inflammation and ER stress regulation, and balanced the function of saturated and unsaturated long-chain fatty acid in adipose tissue.</p></div>","PeriodicalId":100761,"journal":{"name":"Journal of Cellular Immunotherapy","volume":"4 1","pages":"Pages 22-25"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jocit.2018.09.012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83191201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diabetic patients on Rutherford's stage 5 is the best indication of stem cell therapy in peripheral artery disease: A retrospective study on 367 patients","authors":"Bertrand Lehalle ,&nbsp;Philippe Jan ,&nbsp;Jean-François Stoltz","doi":"10.1016/j.jocit.2018.09.005","DOIUrl":"10.1016/j.jocit.2018.09.005","url":null,"abstract":"<div><p>Stem cell therapy currently seems to be pushing back limits to science. However, on the clinician's perspective, many results must be relativized, since efficiency and relevancy criterion are far away from practical reality. Therefore, we took an interest in the potential use of stem cell therapy in patients with peripheral artery disease. Through a retrospective clinical study, we identified a group of patients in which cell therapy could potentially be of interest, in terms of public health and cost. We demonstrated that this group corresponds to diabetic patients on Rutherford's stage 5. For this group of patients, we pave the way for a stem cell therapy clinical trial, associated with conventional care, with wound healing time and reintervention rate as endpoints.</p></div>","PeriodicalId":100761,"journal":{"name":"Journal of Cellular Immunotherapy","volume":"4 1","pages":"Pages 18-21"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jocit.2018.09.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81204746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}