The miRNA binding site SNP in the 3′ UTR of NBS1 is inversely associated with breast cancer risk

Journal of Cellular Immunotherapy Pub Date : 2018-12-01 DOI:10.1016/j.jocit.2018.09.013

Nafezeh Ghanei , Kamran Ghaedi , Masoumeh Mansouri bidakani , Mohammad Fazilati , Habibollah Nazem

{"title":"The miRNA binding site SNP in the 3′ UTR of NBS1 is inversely associated with breast cancer risk","authors":"Nafezeh Ghanei , Kamran Ghaedi , Masoumeh Mansouri bidakani , Mohammad Fazilati , Habibollah Nazem","doi":"10.1016/j.jocit.2018.09.013","DOIUrl":null,"url":null,"abstract":"<div><h3>Aim</h3><p>Nijmegen breakage syndrome 1 (<em>NBS1</em>), also known as <em>NBN</em>, participates in cellular response to DNA damage and maintaining genomic stability. Functional polymorphisms located at a microRNA binding site in the 3′ UTR of <em>NBS1</em> have been frequently studied in various cancers, but the results are inconsistent. The aim of the present study was to test a possible association between breast cancer risk and the <em>NBS1</em> genetic variant, <em>NBS1</em> rs2735383 541G > C, by performing a case–control study in an Iranian population.</p></div><div><h3>Materials & methods</h3><p>Allele-specific primer PCR method was utilized to genotype the genetic variant in 129 women with breast cancer and 117 healthy controls. The association tests with risk of breast cancer were examined by logistic regression.</p></div><div><h3>Results</h3><p>The <em>NBS1</em> rs2735383 541G > C polymorphism was inversely associated with breast cancer (GC + CC vs GG, OR = 0.42, 95% C.I = 0.25–0.72, <em>P</em> value = 0.001; C vs G allele, OR = 0.43, 95% C.I = 0.29–0.62, <em>P</em> value < 0.001) and also lymph node metastasis (GC + CC vs GG, OR = 0.23, 95% C.I = 0.11–0.52, <em>P</em> value < 0.001).</p></div><div><h3>Conclusion</h3><p>The rs2735383 variant may be a genetic modifier for breast cancer development in Iranians.</p></div>","PeriodicalId":100761,"journal":{"name":"Journal of Cellular Immunotherapy","volume":"4 2","pages":"Pages 75-78"},"PeriodicalIF":0.0000,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jocit.2018.09.013","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cellular Immunotherapy","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352177518300189","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Aim

Nijmegen breakage syndrome 1 (NBS1), also known as NBN, participates in cellular response to DNA damage and maintaining genomic stability. Functional polymorphisms located at a microRNA binding site in the 3′ UTR of NBS1 have been frequently studied in various cancers, but the results are inconsistent. The aim of the present study was to test a possible association between breast cancer risk and the NBS1 genetic variant, NBS1 rs2735383 541G > C, by performing a case–control study in an Iranian population.

Materials & methods

Allele-specific primer PCR method was utilized to genotype the genetic variant in 129 women with breast cancer and 117 healthy controls. The association tests with risk of breast cancer were examined by logistic regression.

Results

The NBS1 rs2735383 541G > C polymorphism was inversely associated with breast cancer (GC + CC vs GG, OR = 0.42, 95% C.I = 0.25–0.72, P value = 0.001; C vs G allele, OR = 0.43, 95% C.I = 0.29–0.62, P value < 0.001) and also lymph node metastasis (GC + CC vs GG, OR = 0.23, 95% C.I = 0.11–0.52, P value < 0.001).

Conclusion

The rs2735383 variant may be a genetic modifier for breast cancer development in Iranians.

查看原文本刊更多论文

NBS1 3 ' UTR中的miRNA结合位点SNP与乳腺癌风险呈负相关

nijmegen broken syndrome 1 (NBS1)，又称NBN，参与细胞对DNA损伤的反应，维持基因组的稳定性。位于NBS1 3 ' UTR的microRNA结合位点的功能多态性在各种癌症中经常被研究，但结果不一致。本研究的目的是通过在伊朗人群中进行病例对照研究，测试乳腺癌风险与NBS1基因变异NBS1 rs2735383 541G > C之间的可能关联。材料,方法采用等位基因特异性引物PCR方法对129例乳腺癌患者和117例健康对照者进行基因分型。通过logistic回归检验与乳腺癌风险的关联试验。结果:NBS1 rs2735383 541G >C多态性与乳腺癌呈负相关(GC + CC vs GG, OR = 0.42, 95% ci = 0.25-0.72, P值 = 0.001;C和G等位基因,或者 = 0.43,95% C.I = 0.29 - -0.62,P值& lt;0.001)和淋巴结转移(GC + CC vs GG, OR = 0.23, 95% ci = 0.11-0.52, P值 < 0.001)。结论rs2735383变异可能是伊朗人乳腺癌发生的基因修饰因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Cellular Immunotherapy

自引率

0.00%

发文量