International Journal of Chemical and Analytical Science最新文献

{"title":"Antibacterial activity of pigment produced from Micrococcus luteus KF532949","authors":"Kaliappan Umadevi,&nbsp;Marimuthu Krishnaveni","doi":"10.1016/j.ijcas.2013.08.008","DOIUrl":"10.1016/j.ijcas.2013.08.008","url":null,"abstract":"<div><h3>Aim</h3><p>The problems of synthetic pigment causing toxicity and carcinogenicity in the human body decrease its use. Therefore, interest in natural pigment production is increasing. Therefore, the marine environment has recently become an attractive research subject for many investigations, because of its rich biodiversity. Attempt has been initiated to isolate <em>Micrococcus luteus</em> from marina beach water.</p></div><div><h3>Methods</h3><p>The sea water sample isolated was allowed to grow in a Zobell marine agar medium and incubated at 37 °C for one week. The isolated strain from marine source was confirmed by 16S rRNA sequencing. The GC content was found to be 58.6%. Phylogenetic tree analysis was performed to know the sequences that are closely related. The strain was assessed for pigment production in Zobell marine broth and incubated at 37 °C for two days. The swab sticks collected from hospital were streaked directly on the labelled agar plates such as eosin methylene blue, MacConkey, nutrient, blood, mannitol salt agar and incubated at 37 °C for 24 h and characterized. The antibacterial activity of crude pigment was tested against wound isolates.</p></div><div><h3>Results</h3><p>The individual yellow colour colonies obtained was sequenced, submitted to gene bank under the accession number KF532949. Phylogenetic analysis revealed, that the identified strain <em>M. luteus</em> is closely related to various <em>Micrococcus</em> sp. The crude pigment showed promising results against <em>Staphylococcus</em> sp., <em>Klebsiella</em> sp., <em>Pseudomonas</em> sp. isolated from wound.</p></div><div><h3>Conclusion</h3><p>From this result, we can conclude that the isolated strain <em>M. luteus</em> is able to act against both gram positive and gram negative bacteria.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 3","pages":"Pages 149-152"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.08.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89339604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A facile synthesis of Methyl-2-(N-ethyl-N-phenylamino)naphthyridine-3-carboxylates","authors":"Kishore Kumar Anantoju ,&nbsp;Giri Tharikoppula ,&nbsp;Laxminarayana Eppakayala ,&nbsp;Thirumala Chary Maringanti","doi":"10.1016/j.ijcas.2013.07.008","DOIUrl":"10.1016/j.ijcas.2013.07.008","url":null,"abstract":"<div><h3>Objective</h3><p>To develop an efficient method for the synthesis of Methyl-2-(N-ethyl-N-phenylamino)naphthyridine-3-carboxylates which is suitable for different naphthyridines.</p></div><div><h3>Methods</h3><p>All reagents used were commercial grade, ¹H NMR spectra were obtained on a varian 400 MHz instrument with TMS as internal standard and chemical shifts are expressed <em>δ</em> ppm solvent used in DMSO-d₆ &amp; CDCl₃ and LC–MS spectrum on a Waters LC–MS spectrometer operating at 70 ev. TLC is performed with E-Merch precoated silica gel plates (60F-254) with iodine as a developing agent. Acme, India silica gel, 60–120 mesh for column chromatography is used. All compounds are purified by column chromatography by using Silica gel (60–120 mesh) eluted with (50:1) dichloromethane in methanol.</p></div><div><h3>Conclusions</h3><p>We have developed an efficient protocol for the synthesis of Methyl-2-(N-ethyl-N-phenylamino)naphthyridine-3-carboxylates.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 3","pages":"Pages 146-148"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.07.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85194673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An isocratic solvent-free mobile phase HPLC-PDA analysis of clenbuterol and ractopamine","authors":"Naoto Furusawa","doi":"10.1016/j.ijcas.2013.07.002","DOIUrl":"10.1016/j.ijcas.2013.07.002","url":null,"abstract":"<div><p>This paper describes a reserved-phase HPLC method for detecting clenbuterol (CB) and ractopamine (RP) using an isocratic solvent-free mobile phase. The separations were performed a Kaseisorb^® LC C1-300-5 (100 × 4.6 mm, 5 μm) with 0.05 mol/L octane sulfonic acid mobile phase and a photodiode array detector. The total run time was &lt;5 min. The system suitability was well within the international acceptance criteria. The detection limits were 0.68 ng/mL for CB and 1.24 ng/mL for RP, respectively. A harmless method for simultaneous detecting CB and RP was developed and may be further applied to the quantification in foods.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 3","pages":"Pages 169-173"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.07.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81004487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New compound 6,7-dimethoxy-2-methylisoquinolinium from Indian medicinal plant Annona squamosa L.","authors":"Jayendra,&nbsp;Yatendra Kumar","doi":"10.1016/j.ijcas.2013.08.005","DOIUrl":"10.1016/j.ijcas.2013.08.005","url":null,"abstract":"<div><h3>Objectives</h3><p><em>Annona squamosa</em> L. is a widely used Indian medicinal plant for the cure of deadly disease, diabetes. In recent decades, a great no. of chemical and pharmacological studies have been done on <em>A. squamosa</em> L. and still more research is required in order to search its bioactive components.</p></div><div><h3>Methods</h3><p>Bioassay dependent fractionation &amp; isolation technique is used &amp; repeated column chromatography was utilized to get pure compounds.</p></div><div><h3>Results</h3><p>Structure elucidation for <strong>compound A</strong> &amp; <strong>compound B</strong> has been done using Mass, NMR, IR, UV, 1D &amp; 2D NMR techniques.</p></div><div><h3>Conclusion</h3><p>In this paper we report one new compound from Indian medicinal plant <em>A. squamosa</em> L. which is characterized as 6,7-dimethoxy-2-methylisoquinolinium &amp; is obtained from the twigs of the plant in its chloroform fraction. One known compound Rutin is also obtained from <em>n</em>-butanol fraction and is reported for the first time from this plant which is characterized as 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-{{(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyltetrahydro-2H-pyran-2-yloxy}methyl}tetrahydro-2H-pyran-2-yloxy]-4H-chromen-4-one.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 3","pages":"Pages 161-168"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.08.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77639774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of photodegradation efficiency on semiconductor immobilized clay photocatalyst by using probit model approximation","authors":"Jaka Nugraha ,&nbsp;Is Fatimah","doi":"10.1016/j.ijcas.2013.07.005","DOIUrl":"https://doi.org/10.1016/j.ijcas.2013.07.005","url":null,"abstract":"<div><p>Photodegradation process and mechanism is a relative new and eco-friendly process to recycle and reuse of water. Some investigation concerned with effort to increase photocatalyst activity by immobilizing semiconductor oxide such as ZnO in clay as solid support. It was related to that photodegradation was directed by adsorption mechanism. However sometimes the activity of a photocatalyst is not linearly correlated with adsorption mechanism. In this research evaluation on photoactivity of ZnO-hectorite (ZnO/CTMA/Hectorite) and TiO₂-montmorillonite based material using statistical approximation compared to the linear model is studied. The model is evaluated for photodegradation of phenol solution. Some beneficial results of the probit model are (i) the guarantee of activity prediction value in the range of 0–100% (ii) interaction of adsorption and photodegradation mechanism is well described.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 2","pages":"Pages 125-130"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.07.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91726656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assay of some non-steroidal anti-inflammatory drugs with ammonium hexanitratocerate (IV) reagent","authors":"Vinod Kumar, B. Singh, I. C. Shukla","doi":"10.1016/J.IJCAS.2013.03.007","DOIUrl":"https://doi.org/10.1016/J.IJCAS.2013.03.007","url":null,"abstract":"","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"6 1","pages":"115-119"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76983155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invitro free radical scavenging, cytotoxic and acetylcholinesterase inhibitory activities of Leucas martinicensis","authors":"R. Ramalingam, Ravinder Nath ANISETTI, B. Madhavi, M. Nagulu","doi":"10.1016/J.IJCAS.2013.04.005","DOIUrl":"https://doi.org/10.1016/J.IJCAS.2013.04.005","url":null,"abstract":"","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"30 1","pages":"91-95"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85447343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New HPLC method to detect amitriptyline in the blood of rats on combination treatment","authors":"R. Farag, M. Darwish, W. Fathy, H. Hammad","doi":"10.1016/J.IJCAS.2013.06.001","DOIUrl":"https://doi.org/10.1016/J.IJCAS.2013.06.001","url":null,"abstract":"","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"35 1","pages":"120-124"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87941345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidermatophytic activity of Eclipta prostrata L. against human infective Trichophyton and Microsporum spp.","authors":"Nagabhushan, K. Raveesha, D. L. Shrisha","doi":"10.1016/J.IJCAS.2013.05.003","DOIUrl":"https://doi.org/10.1016/J.IJCAS.2013.05.003","url":null,"abstract":"","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"75 1","pages":"136-138"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83451142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The development and validation of a Thin Layer Chromatography densitometry method for the analysis of diclofenac sodium tablets","authors":"Eliangiringa Kaale ,&nbsp;Bugusu C. Nyamweru ,&nbsp;Vicky Manyanga ,&nbsp;Mhina Chambuso ,&nbsp;Thomas Layloff","doi":"10.1016/j.ijcas.2013.05.001","DOIUrl":"10.1016/j.ijcas.2013.05.001","url":null,"abstract":"<div><h3>Aims</h3><p>Diclofenac sodium is a Nonsteroidal Anti-Inflammatory Drug (NSAID) with anti-inflammatory, antipyretic, and analgesic action as a result of the blockade of prostaglandin synthesis by inhibition of the cyclooxygenase enzyme. Analysis of the active pharmaceutical ingredient as well as finished pharmaceutical product is of vital importance to help ensure that good quality products are manufactured and supplied to the patient end users. The aim of the study was to develop a simple and rapid method to enable timely release of products.</p></div><div><h3>Methods</h3><p>The method was developed using a mobile phase prepared with environment-friendly analytical grade solvents: toluene, acetone and glacial acetic acid (10:15:0.2 v/v/v). Diclofenac sodium reference material and samples were chromatographed using pre-coated TLC silica gel 60 F₂₅₄ glass plates with a saturation time of 25 minutes and a densitometer detection wavelength of 284 nm in the reflectance absorbance mode. Detection and part of analysis was done using densitometer TLC Scanner 3 operated with Wincats (version 1.4.3) planar chromatograph software.</p></div><div><h3>Results</h3><p>A Thin Layer Chromatography (TLC) method for the qualitative and quantitative analysis of diclofenac sodium tablets was developed and validated according to ICH and USP guidelines. The <em>R</em>_f of diclofenac sodium was at 0.60 and the method was repeatable, robust with good selectivity and specificity. Regression functions were established over the range of 250–600 ng/spot with <em>r</em>² of 0.993 and 0.999 for the linear and polynomial regressions respectively. The accuracy at nominal concentration was found to be 100.2%, and the results of the assay of three brands for diclofenac sodium tablets were found to meet the USP 95–105% assay limits thus demonstrating the usefulness of the method for the assay of these products.</p><p>The developed assay method for diclofenac sodium in tablets is simple, accurate, and inexpensive, with good precision and should be especially useful in resource constrained countries.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 2","pages":"Pages 73-79"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.05.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73162487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}