Antimutagenic and antioxidant activity of Ficus benghalensis stem bark and Moringa oleifera root extract

International Journal of Chemical and Analytical Science Pub Date : 2013-06-01 DOI:10.1016/j.ijcas.2013.03.008

A. Satish , R. Punith Kumar , D. Rakshith , S. Satish , Faiyaz Ahmed

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引用次数: 36

Abstract

Objective

To investigate antimutagenic and antioxidant potency of the aqueous heat treated Ficus benghalensis stem bark (FBH) extract and Moringa oleifera root (MRH) extract against sodium azide in TA100 tester strains of Salmonella typhimurium and their inhibition of microsomal lipid peroxidation (LPO).

Methods

Mutagenicity was assayed by the standard Ames test (standard plate incorporation assay) and antioxidant potency was investigated by employing ex vivo inhibition of lipid peroxidation in liver Microsomes.

Results

Both FBH and MRH showed strong antimutagenic effect on S. typhimurium TA100 strains against sodium azide (NaN₃). IC₅₀ values of aqueous extract of FBH and MRH extracts were 70.24 μg/ml and 99.20 μg/ml respectively. FBH extract showed maximum inhibition of microsomal lipid peroxidation responses than MRH. IC₅₀ values of aqueous extract of FBH and MRH extracts were 80.24 μg/ml and 92 μg/ml respectively. FBH and MRH exhibited a dose dependent antioxidant activity.

Conclusion

The aqueous heat-treated FBH and MRH have antimutagenic as well as antioxidant activity. Further studies are in progress to evaluate the effect of both extracts by other antioxidant and antimutagenic assays and to identify the factors responsible for these activities.

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榕树茎皮和辣木根提取物的抗诱变和抗氧化活性

目的研究经水热处理的红ficus benghalensis茎皮(FBH)提取物和辣木根(MRH)提取物对鼠伤寒沙门氏菌TA100试验菌株叠氮化钠的抗诱变和抗氧化作用及其对微粒体脂质过氧化(LPO)的抑制作用。方法采用标准Ames试验(标准平板掺入法)检测其致突变性，并采用体外抑制肝微粒体脂质过氧化的方法研究其抗氧化能力。结果FBH和MRH对鼠伤寒沙门氏菌TA100菌株对叠氮化钠(NaN3)均有较强的抗诱变作用。FBH和MRH水提液的IC50值分别为70.24 μg/ml和99.20 μg/ml。FBH提取物对微粒体脂质过氧化反应的抑制作用强于MRH。FBH和MRH水提液的IC50值分别为80.24 μg/ml和92 μg/ml。FBH和MRH表现出剂量依赖性的抗氧化活性。结论经水热处理的FBH和MRH具有抗诱变和抗氧化活性。进一步的研究正在进行中，以其他抗氧化和抗诱变试验来评估这两种提取物的作用，并确定这些活性的因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Journal of Chemical and Analytical Science

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