{"title":"Bioactive compound produced from Streptomyces aureofaciens KF532950 and its antimicrobial activity","authors":"Sundaram Vennila, Marimuthu Krishnaveni","doi":"10.1016/j.ijcas.2013.08.007","DOIUrl":null,"url":null,"abstract":"<div><h3>Aim</h3><p>Actinomycetes are gram-positive bacteria, common in nature, play a significant role in biotechnology, as it produces vitamins, enzymes, anti-tumour agents, immune-modifying agents, mainly antibiotic compounds. Various bioactive substances are produced by a wide variety of microorganisms including several species of bacteria and fungi. Hence, it was decided to isolate soil bacteria that is able to produce biologically active substances.</p></div><div><h3>Methods</h3><p>The bioactive compound producing strain was isolated from soil and the same was sequenced, submitted to gene bank for accession number. Related species was known by tree construction. The bacterial isolates from wound were tested for its susceptibility against bioactive compound isolated from <em>Streptomyces aureofaciens</em> by disc diffusion assay. The produced compound was subjected to SDS-PAGE analysis for molecular weight determination.</p></div><div><h3>Results</h3><p>The isolated strain was obtained accession number KF532950, it contains high GC content of 63.6%. The bioactive compound produced from <em>Streptomyces aureofaciens</em> showed positive result against <em>Staphylococcus</em> spp. and <em>Pseudomonas</em> spp. and its molecular weight ranged from 14.3 to 97.4 Kd.</p></div><div><h3>Conclusion</h3><p>From the results obtained, it is concluded that, our strain produces bioactive compound which may be further characterized for its properties.</p></div>","PeriodicalId":100693,"journal":{"name":"International Journal of Chemical and Analytical Science","volume":"4 3","pages":"Pages 153-155"},"PeriodicalIF":0.0000,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcas.2013.08.007","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Chemical and Analytical Science","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0976120913000454","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Aim
Actinomycetes are gram-positive bacteria, common in nature, play a significant role in biotechnology, as it produces vitamins, enzymes, anti-tumour agents, immune-modifying agents, mainly antibiotic compounds. Various bioactive substances are produced by a wide variety of microorganisms including several species of bacteria and fungi. Hence, it was decided to isolate soil bacteria that is able to produce biologically active substances.
Methods
The bioactive compound producing strain was isolated from soil and the same was sequenced, submitted to gene bank for accession number. Related species was known by tree construction. The bacterial isolates from wound were tested for its susceptibility against bioactive compound isolated from Streptomyces aureofaciens by disc diffusion assay. The produced compound was subjected to SDS-PAGE analysis for molecular weight determination.
Results
The isolated strain was obtained accession number KF532950, it contains high GC content of 63.6%. The bioactive compound produced from Streptomyces aureofaciens showed positive result against Staphylococcus spp. and Pseudomonas spp. and its molecular weight ranged from 14.3 to 97.4 Kd.
Conclusion
From the results obtained, it is concluded that, our strain produces bioactive compound which may be further characterized for its properties.
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