Interdisciplinary Medicine最新文献

{"title":"Listeria monocytogenes membrane vesicles disrupting intestinal epithelial barrier function via modulating macrophage inflammatory responses","authors":"Yao Lei,&nbsp;Kehan Chen,&nbsp;Mingyuan Tang,&nbsp;Qiuyang Zhang,&nbsp;Jie Yu,&nbsp;Yunwen Zhang,&nbsp;Tian Tang,&nbsp;Chuan Wang","doi":"10.1002/INMD.20240066","DOIUrl":"https://doi.org/10.1002/INMD.20240066","url":null,"abstract":"<p>Bacterial extracellular vesicles (BEVs) are actively secreted nanostructures that play a unique role in the pathogenesis of bacterial infections. In this study, we established an intestinal epithelial cell (IEC)/macrophage coculture system and found that <i>Listeria monocytogenes</i> (LM) membrane vesicles (MVs) acted on macrophages and caused intestinal barrier disruption. Further studies revealed that LM MVs not only induced the M1-type polarization in macrophages through the MAPK signaling pathway but also activated AIM2 and NLRP3 inflammasomes in macrophages to induce pyroptosis. The inflammatory factors IFN-α and TNF-α released by macrophages can bind to IFNAR and TNFR in IECs and activate the JAK-STAT3 signaling pathway, disrupting the intestinal barrier. Our study illustrates the role and mechanism of LM MVs in disrupting the intestinal barrier by regulating the inflammatory response of macrophages, which helps understand the function of LM MVs, explains the mechanism of LM-intestinal infection, and may provide new targets for the treatment of intestinal invasive infections with LM.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"3 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/INMD.20240066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144220134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of microbiome-derived amino acids metabolites in shaping the glioma immunosuppressive microenvironment","authors":"Qianquan Ma,&nbsp;Zhihao Song,&nbsp;Chenlong Yang,&nbsp;Zijin Zhao,&nbsp;Guodong Tang,&nbsp;Jia You,&nbsp;Chong Zeng,&nbsp;Jun Yang,&nbsp;Qing Liu,&nbsp;Haoyu Li,&nbsp;Wei Huang","doi":"10.1002/INMD.20240070","DOIUrl":"https://doi.org/10.1002/INMD.20240070","url":null,"abstract":"<p>Gliomas are the most common intracranial tumors characterized by highly malignant behavior. In addition to genetic and epigenetic mutations, the unique cancer microenvironment (CME) plays a pivotal role in glioma progression and resistance to therapy. Among the critical factors in the glioma CME, amino acid metabolism stands out for its significant influence, with specific amino acids suppressing anti-cancer immune responses and promoting an immunosuppressive environment. The human microbiota affect host metabolism and immune functions, with disruptions in microbiota homeostasis leading to metabolic alterations and immune dysfunction in various diseases. Emerging evidence highlights the role of microbiota-derived metabolites, including amino acids, in reprogramming the glioma CME and modulating oncogenic signaling pathways. This review examines the influence of the gut microbiome on specific amino acid metabolism—namely, tryptophan, tyrosine, arginine, and branched-chain amino acids—and evaluates the potential roles of microbiome-derived metabolites in the prognosis and diagnosis of glioma.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"3 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/INMD.20240070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144220240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"mRNA therapy: A new frontier in regenerative medicine","authors":"Ding-Ding Xue,&nbsp;Yue Zhang,&nbsp;An-Ran Shen,&nbsp;Tao-Tao Tang,&nbsp;Bi-Cheng Liu,&nbsp;Lin-Li Lv","doi":"10.1002/INMD.20240054","DOIUrl":"https://doi.org/10.1002/INMD.20240054","url":null,"abstract":"<p>Tissue regeneration has raised intensive attention due to its great significance in overcoming various diseases resulting from different injuries. Since the COVID-19 pandemic, mRNA therapeutics have emerged as innovative strategies in the prevention and treatment of diseases due to their unique advantages. Compared to traditional regenerative strategies, mRNA therapy offers rapid translation into proteins with low production cost and high modifiability. Herein, we discuss the progress in the key processes of mRNA therapy, focusing on therapeutic mRNA modification and delivery carriers. The preclinical and clinical studies of mRNA therapeutics for regeneration of cardiac, lung, liver, kidney, locomotor system, skin lesions and neurological disorders were summarized comprehensively. Developing strategies to reduce immunogenicity and off-target effects, as well as optimization of the delivery system may accelerate the pace of clinical translation.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/INMD.20240054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting the crosstalk between glutamine metabolism and tumor immune microenvironment for lung cancer immunotherapy","authors":"Jingyang Li,&nbsp;Li Xiang,&nbsp;Shaohui Wang,&nbsp;Yingrui Zhang,&nbsp;Shiyi Zhao,&nbsp;Di Zhang,&nbsp;Xianli Meng,&nbsp;Yi Zhang,&nbsp;Jin-Ming Lin","doi":"10.1002/INMD.20240069","DOIUrl":"https://doi.org/10.1002/INMD.20240069","url":null,"abstract":"<p>Lung cancer remains one of the deadliest cancers globally, which may be attributed in part to a limited understanding of its development. Therefore, exploration of the fundamental processes of lung cancer and innovative therapies is imminent. Recently, lung cancer immunotherapy has become a crucial topic of interest, and it pertains to the metabolism in lung tumor microenvironment. In particular, glutamine metabolism plays a pivotal role in lung cancer immunotherapy. This review summarizes the crosstalk between glutamine metabolism and the lung tumor immune microenvironment, and explores potential strategies of glutamine metabolism as an enhancement of lung cancer immunotherapy. It also discusses the potential of traditional Chinese medicine in regulating glutamine metabolism in lung cancer immunotherapy. This review may offer valuable insights and promising directions for future studies on this subject.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/INMD.20240069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulating gut microbiota with nanomaterials for cancer immunotherapy","authors":"Tianjian Sha,&nbsp;Yuji Tang,&nbsp;Xinyao Hu,&nbsp;Haibo Zhou,&nbsp;Lang Rao","doi":"10.1002/INMD.20240103","DOIUrl":"https://doi.org/10.1002/INMD.20240103","url":null,"abstract":"<p>Gut microbiota play a key role in cancer immunotherapy and regulating gut microbiota provides new strategies with great potential for cancer therapeutics. Several gut microbiota-based strategies, such as antibiotic treatment and fecal microbiota transplantation, have gained certain achievements in enhancing cancer immunotherapy, while the potential risk of biosafety and the destruction of commensal microbiota limit their further applications. Nanomaterials with excellent capabilities in delivering drugs and modulating bacteria may provide specific solutions for these limitations, and have shown promising potential in manipulating the gut microbiota to boost cancer immunotherapy. In this perspective, we reviewed the breakthroughs of nanomaterials in modulating the gut microbiota and their metabolites to enhance cancer immunotherapy, and further discussed the challenges and opportunities in this emerging field. It is anticipated that this perspective will offer new insights on the rational design of nanomaterials to regulate the gut microbiota for enhanced cancer immunotherapy, fostering clinical translation and ultimately benefiting patients.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/INMD.20240103","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteosarcoma cells-derived exosomal YES1 promote tumor progression via MAPK pathway","authors":"Zihua Li,&nbsp;Qingjing Chen,&nbsp;Yi Zhang,&nbsp;Zhanhui Ye,&nbsp;Yixian Song,&nbsp;Yiwei Zhang,&nbsp;Chenzheng Gu,&nbsp;Jia Tan,&nbsp;Yunfeng Yang,&nbsp;Anquan Shang","doi":"10.1002/INMD.20240072","DOIUrl":"https://doi.org/10.1002/INMD.20240072","url":null,"abstract":"<p>Osteosarcoma (OS), the most common primary bone tumor in children and young adults, poses significant clinical challenges. In this study, we investigated the role of exosomes—key mediators of intercellular communication—in OS progression and identified potential therapeutic targets. Functional assays in cellular and animal models demonstrated that OS-derived exosomes markedly enhanced tumor proliferation, migration, and invasion. Data-independent acquisition-based mass spectrometry analysis revealed a notable enrichment of the proto-oncogene tyrosine-protein kinase YES1 in these exosomes. Bioinformatics and clinical investigations further showed that YES1 is overexpressed in multiple cancers and correlates with poor outcomes; specifically, in OS, elevated YES1 levels were associated with reduced overall survival and adverse prognoses. PCR, Western blotting, and immunohistochemistry (IHC) of clinical samples confirmed its high expression in OS tissues relative to normal counterparts. Mechanistic studies using YES1 knockdown (shYES1) and overexpression (OE YES1) models, coupled with exosome supplementation and pathway modulators, revealed a critical role for ERK phosphorylation in mediating YES1-driven oncogenic behaviors. In shYES1 cells, tumor proliferation, migration, and invasion were significantly impaired, partially rescued by exosome supplementation, and further suppressed by the inhibitor of MAPK. Conversely, in OE YES1 cells, these malignant phenotypes were markedly enhanced, exacerbated by exosomes, and further promoted by the agonist of MAPK. Western blot analyses supported these observations, showing reduced p-MEK and p-ERK but increased p-p38 and p-JNK upon YES1 knockdown, with opposite trends in the OE YES1 group; these phosphorylation changes were reversed or attenuated by EGF/IL-1beta or U0126/SB-203580 treatment, respectively, without altering total protein levels of MEK, JNK, p38, and ERK. Finally, multiplex IHC validated bioinformatics predictions, demonstrating that YES1 expression is closely linked to immune-related pathways in OS tissues. Collectively, these findings underscore the pivotal role of exosomal YES1 in OS progression and tumor immunology, highlighting its promise as a biomarker and therapeutic target. By illuminating its function in cancer behavior and immune interactions, this study offers novel insights into improving patient outcomes in OS.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/INMD.20240072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A facile automated multiple-well platform of immunized tumor organoid cultures in ECM-mimicked hydrogels for chem-immunotherapy evaluation","authors":"Ruizhi Tang,&nbsp;Xi-Qiu Liu","doi":"10.1002/INMD.20240083","DOIUrl":"https://doi.org/10.1002/INMD.20240083","url":null,"abstract":"<p>Breast cancer often develops drug resistance during chemotherapy, and immunotherapeutic agents always exhibit ineffectiveness when used as a single treatment; thus, it is necessary to develop a chemo-immunotherapy strategy in clinics. However, there is still a challenge to evaluate the chemo-immunotherapy efficacy by conventional 2D cell culture and animal models. In this study, we developed a facile automated multiple-well platform for fabricating tumor associated macrophages (TAMs)-immunized breast cancer organoids in alginate hydrogels. An automated robotic microinjection system was used for building alginate hydrogels in situ and seeding mixed cell suspensions of breast cancer cells and TAMs into hydrogels to form organoids. The induced drug resistance to epirubicin was observed in breast cancer organoids with TAMs, but it could be inhibited by targeted immunotherapy PLX3397. The synergistic effects were also evaluated in several co-administration strategies of PLX3397 in combination with epirubicin. The RNA-seq and quantitative polymerase chain reaction were further used to examine gene transcription levels in the co-administration and find out three genes (IL6, CD37, and GLS2) with significant differences, which were involved in the tumor necrosis factor signaling, PI3K-Akt signaling and epidermal growth factor receptor tyrosine kinase inhibitor resistance pathway. The results demonstrated that the automated multiple-well platform showed the potential to replace the conventional bulk culture method in evaluating the therapeutic effects of chem-immunotherapy.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/INMD.20240083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between selenium and viral diseases","authors":"Jiali Li,&nbsp;Jia Lai,&nbsp;Yuhui Zeng,&nbsp;Bing Zhu,&nbsp;Yinghua Li","doi":"10.1002/INMD.20240074","DOIUrl":"https://doi.org/10.1002/INMD.20240074","url":null,"abstract":"<p>Selenium (Se) is a trace element that fulfills vital functions in the organism. Over the past few years, numerous studies have revealed the profound connection between Selenium and its therapeutic potential in addressing various viral infectious diseases. Selenium can boost the activity of immune cells and counteract oxidative damage caused by free radicals to prevent the replication of viruses. Studies have shown that Selenium protects against a wide range of viruses such as DNA viruses, hepatitis B virus, RNA viruses, human immunodeficiency virus and coxsackie virus. Selenium is probably one of the research directions for antiviral drugs in the future. This review summarizes the use of Selenium in antiviral therapy and its research progress.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/INMD.20240074","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Table of Content","authors":"","doi":"10.1002/inmd.12154","DOIUrl":"https://doi.org/10.1002/inmd.12154","url":null,"abstract":"<p>No abstract is available for this article.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.12154","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143119062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbial 3α-hydroxysteroid dehydrogenase induces depression in males via degrading testosterone (1/2025)","authors":"Di Li,&nbsp;Jingru Cheng,&nbsp;Wei Zhang,&nbsp;Pengfei Cheng,&nbsp;Yuan He,&nbsp;Jinghan Jia,&nbsp;Chao Wang,&nbsp;Zhongchun Liu,&nbsp;Zhou Sun,&nbsp;Jinxi Wang","doi":"10.1002/inmd.12153","DOIUrl":"https://doi.org/10.1002/inmd.12153","url":null,"abstract":"<p>This image illustrates our paper on how gut microbiota induces depression via degrading testosterone in man. The human in the center of the picture represents the emotions of men. The blue background represents the emotion of depression. Surrounding men are different species of gut bacteria. The small balls near bacteria represent testosterone molecules. This indicates gut microbiota degrades testosterone via produces 3α-HSD enzyme. The whole picture demonstrates that the 3α-HSD enzyme produced by gut microbiota decreases testosterone in man via degradation ability and induces depression, which indicates that 3α-HSD enzyme are potential targets for depression. This personification visualizes the core concept of our paper.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.12153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143119059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}