Interdisciplinary Medicine最新文献

{"title":"TROP2-guided AIE-luminogen nanoparticles overcome sacituzumab govitecan resistance via mitochondrial oxidative stress and immunogenic cell death in triple-negative breast cancer (6/2025)","authors":"Nisha Wu,&nbsp;Shujuan Ma,&nbsp;Xiaohong Liu,&nbsp;Min Zhao,&nbsp;Na Sun,&nbsp;Rui Wang,&nbsp;Shanlin Huang,&nbsp;Minghao Sun,&nbsp;Ying Liu,&nbsp;Jun Jiang,&nbsp;Mengyuan Wang,&nbsp;Yi Zhang,&nbsp;Ying Gong,&nbsp;Xiaowei Qi","doi":"10.1002/inmd.70079","DOIUrl":"https://doi.org/10.1002/inmd.70079","url":null,"abstract":"<p>Schematic of AIE780-SG NPs for NIR FLI-Guided Photo-Enhanced Chemotherapy in Drug-Resistant TNBC. AIE780-SG (hRS7-modified) targets TROP2 on TNBC cells to facilitate mitochondrial internalization. NIR illumination induces MICA/B release, recruiting and activating NK cells via MICA/B-NKG2D and hRS7-CD16 interactions, and enhancing secretion of cytotoxic molecules (GZMB, perforin, IFN-γ). This nanomaterial precisely targets tumor cells, and its dual immune-enhancing and direct DNA-damaging mechanism boosts therapeutic efficacy against resistant TNBC.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"3 6","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70079","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TROP2-guided aggregation-induced emission-luminogen nanoparticles overcome sacituzumab govitecan resistance via mitochondrial oxidative stress and immunogenic cell death in triple-negative breast cancer","authors":"Nisha Wu,&nbsp;Shujuan Ma,&nbsp;Xiaohong Liu,&nbsp;Min Zhao,&nbsp;Na Sun,&nbsp;Rui Wang,&nbsp;Shanlin Huang,&nbsp;Minghao Sun,&nbsp;Ying Liu,&nbsp;Jun Jiang,&nbsp;Mengyuan Wang,&nbsp;Yi Zhang,&nbsp;Ying Gong,&nbsp;Xiaowei Qi","doi":"10.1002/inmd.70073","DOIUrl":"https://doi.org/10.1002/inmd.70073","url":null,"abstract":"<p>Chemoresistance and immune evasion constitute formidable obstacles in triple-negative breast cancer (TNBC) therapy, exacerbated by the suboptimal pharmacokinetics and acquired resistance of antibody–drug conjugates (ADCs). Herein, we describe the engineering of antibody-guided nanoparticles (NPs) co-delivering the trophoblast cell-surface antigen 2 (TROP2)-targeting ADC sacituzumab govitecan (SG) with the mitochondria-directed near-infrared (NIR) photosensitizer AIE780. These AIE780–SG nanoconstructs exploit hRS7 antibody–mediated targeting to preferentially accumulate in TROP2-overexpressing, SG-resistant TNBC cells and patient-derived organoid. Upon NIR irradiation, AIE780 induces a mitochondrial redox imbalance via localized reactive oxygen species generation, precipitating tumor-selective immunogenic cell death (ICD) through membrane destabilization and oxidative necrosis. Concurrently, SG undergoes acid-responsive cleavage to release SN-38—a potent topoisomerase I inhibitor—and the hRS7 antibody fragment, which orchestrates natural killer (NK) cell recruitment and activation. In murine TNBC xenograft models, AIE780–SG NPs achieved synergistic chemophotodynamic tumor eradication, surmounting SG resistance and revitalizing antitumor immunity. This TROP2-targeted, light-sensitive theranostic platform offers a multimodal paradigm to potentiate ADC efficacy and reprogram the immunosuppressive TNBC microenvironment, heralding a novel strategy against SG-resistant TNBC. A dual-therapeutic nanoplatform combining targeted ADC delivery with photodynamic induction of ICD overcomes SG resistance in TNBC and amplifies NK cell-mediated antitumor responses.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"3 6","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Illumina NovaSeq 6000 and GeneMind SURFSeq 5000 platforms for single-cell spatial transcriptomics of mouse brain and lung","authors":"Qianwen Wang,&nbsp;Lin Deng,&nbsp;Shuangbin Xu,&nbsp;Pingfan Guo,&nbsp;Hongyuan Zhu,&nbsp;Haoxing Ge,&nbsp;Yuyan Gong,&nbsp;Guohui Du,&nbsp;Kaijia Huang,&nbsp;Chenyi Su,&nbsp;Rui Wang,&nbsp;Yiyan Qiu,&nbsp;Guangchuang Yu","doi":"10.1002/inmd.70067","DOIUrl":"10.1002/inmd.70067","url":null,"abstract":"<p>Single-cell spatial transcriptomics enables comprehensive gene expression profiling with precise cellular localization within tissue architecture. To systematically evaluate the compatibility and performance of alternative sequencing platforms for this application, we directly compared Illumina NovaSeq 6000 and GeneMind SURFSeq 5000 using SeekSpace single-cell spatial transcriptomics on mouse brain and lung tissues. Identical cDNA libraries were sequenced on both platforms and processed with a unified bioinformatics pipeline to ensure direct comparability. Across all key sequencing quality metrics—including unique molecular identifier and spatial barcode detection, gene identification, and mapping rates—SURFSeq 5000 demonstrated performance highly similar to NovaSeq 6000, with nearly equivalent quality control metrics and data yields. Integrated downstream analyses—including dimensionality reduction, cell type annotation, spatial mapping, differential gene expression, cell–cell interaction, and spatial hotspot module detection—revealed highly concordant spatial patterns and cellular compositions across both brain and lung tissues. The overlap of differentially expressed genes between platforms reached approximately 65%, and cross-platform cell type assignments showed high reproducibility (Area Under the Receiver Operation Characteristic curve &gt; 0.92). No significant batch effects were observed. These results demonstrate that GeneMind SURFSeq 5000 is a reliable and cost-effective alternative to Illumina NovaSeq 6000 for single-cell spatial transcriptomics, providing comparable data quality and analytical robustness in murine tissue studies. The robust performance of SURFSeq 5000 supports the broader adoption of alternative and affordable sequencing technologies in spatial omics research.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"4 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146139708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Illumina NovaSeq 6000 and GeneMind SURFSeq 5000 platforms for single-cell spatial transcriptomics of mouse brain and lung","authors":"Qianwen Wang,&nbsp;Lin Deng,&nbsp;Shuangbin Xu,&nbsp;Pingfan Guo,&nbsp;Hongyuan Zhu,&nbsp;Haoxing Ge,&nbsp;Yuyan Gong,&nbsp;Guohui Du,&nbsp;Kaijia Huang,&nbsp;Chenyi Su,&nbsp;Rui Wang,&nbsp;Yiyan Qiu,&nbsp;Guangchuang Yu","doi":"10.1002/inmd.70067","DOIUrl":"https://doi.org/10.1002/inmd.70067","url":null,"abstract":"<p>Single-cell spatial transcriptomics enables comprehensive gene expression profiling with precise cellular localization within tissue architecture. To systematically evaluate the compatibility and performance of alternative sequencing platforms for this application, we directly compared Illumina NovaSeq 6000 and GeneMind SURFSeq 5000 using SeekSpace single-cell spatial transcriptomics on mouse brain and lung tissues. Identical cDNA libraries were sequenced on both platforms and processed with a unified bioinformatics pipeline to ensure direct comparability. Across all key sequencing quality metrics—including unique molecular identifier and spatial barcode detection, gene identification, and mapping rates—SURFSeq 5000 demonstrated performance highly similar to NovaSeq 6000, with nearly equivalent quality control metrics and data yields. Integrated downstream analyses—including dimensionality reduction, cell type annotation, spatial mapping, differential gene expression, cell–cell interaction, and spatial hotspot module detection—revealed highly concordant spatial patterns and cellular compositions across both brain and lung tissues. The overlap of differentially expressed genes between platforms reached approximately 65%, and cross-platform cell type assignments showed high reproducibility (Area Under the Receiver Operation Characteristic curve &gt; 0.92). No significant batch effects were observed. These results demonstrate that GeneMind SURFSeq 5000 is a reliable and cost-effective alternative to Illumina NovaSeq 6000 for single-cell spatial transcriptomics, providing comparable data quality and analytical robustness in murine tissue studies. The robust performance of SURFSeq 5000 supports the broader adoption of alternative and affordable sequencing technologies in spatial omics research.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"4 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146139724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting the hypoxia-inducible factor-1 alpha-exoVEGF axis in tumor-associated macrophages overcomes Bacillus Calmette-Guérin immunotherapy resistance","authors":"Yuyang Hou,&nbsp;Yuxi Wang,&nbsp;Qiong Wu,&nbsp;Wenjing Du,&nbsp;Shanzhu Guo,&nbsp;Xinyu Chai,&nbsp;Yinan Wang,&nbsp;Huixin Liang,&nbsp;Hongyan Yuan,&nbsp;Jun Li,&nbsp;Dongmei Yan","doi":"10.1002/inmd.70063","DOIUrl":"10.1002/inmd.70063","url":null,"abstract":"<p>Bacillus Calmette-Guérin (BCG) immunotherapy is limited by resistance in ∼50% of patients, linked to tumor microenvironment (TME) angiogenesis. The work explored if BCG-activated tumor-associated macrophages (TAMs) drive angiogenesis via hypoxia-inducible factor-1α (HIF-1α) to impair BCG efficacy. Results demonstrated that macrophages stimulated with BCG significantly enhanced the proliferation, migration, and tube formation of endothelial cells. Further mechanistic studies revealed that this pro-angiogenic effect was mediated through the activation of the NF-_κB, PI3K/AKT and p38/MAPK signaling pathways. Activation of these pathways subsequently led to the upregulated expression of HIF-1α and vascular endothelial growth factor A (VEGFA). Critically, HIF-1α deficiency in macrophages effectively inhibited BCG-induced angiogenesis without exerting a significant impact on the infiltration of CD8⁺ T cells or B cells. Additionally, BCG stimulation enhanced the secretion of VEGF encapsulated in exosomes in an HIF-1α-dependent manner, and these VEGF-enriched exosomes further facilitated the activation of endothelial cell functions. In conclusion, the present study confirms that BCG can promote tumor angiogenesis by activating macrophages to induce the release of exosome-derived VEGF through an HIF-1α-dependent pathway. Targeting the HIF–1α-exosome-derived VEGF (HIF-1α–exoVEGF) axis holds promise as a potential strategy to overcome BCG resistance and improve its therapeutic efficacy.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"4 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146140073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endothelial derived microvesicles modulate cerebrovascular and brain aging via MiR-17-5p and the biomarker potential in vascular aging","authors":"Huiting Zhang,&nbsp;Xiaobing Xu,&nbsp;Zi Xie,&nbsp;Yuhui Zhao,&nbsp;Yanyu Chen,&nbsp;Ye Ye,&nbsp;Yuping Yang,&nbsp;Bin Zhao,&nbsp;Wangtao Zhong,&nbsp;Xiaotang Ma","doi":"10.1002/inmd.70072","DOIUrl":"https://doi.org/10.1002/inmd.70072","url":null,"abstract":"<p>Vascular aging, which is marked by brain endothelial cell (EC) senescence and functional impairment, plays a significant role in diverse age-related cerebrovascular and neurodegenerative disorders. EC-derived microvesicles (EMVs) and exosomes (EEXs) preserve the molecular signatures of their source cells and deliver bioactive cargo to regulate the activity of target cells, but their potentials in vascular aging remain unclear. Here, as indicated by SA-β-gal staining, cerebral blood flow, blood brain barrier function, aging related markers and cognitive ability test, we found that young ECs released EMVs could more effectively alleviate mice cerebrovascular and brain aging than EEXs. Aged ECs released EMVs were more effective than their released EEXs on aggravating mice cerebrovascular and brain aging. We further identified that these EMVs regulated cerebrovascular and brain aging by transferring miR-17-5p and could modulate ECs senescence and functions via miR-17-5p/PI3K/Akt pathway. Plasma EMV concentrations and EMV-miR-17-5p expression were altered in older adults, showing strong associations with reactive oxygen species and vascular aging. Receiver operating characteristic analysis demonstrated the potential of EMVs and EMV-miR-17-5p as diagnostic biomarkers for vascular aging. Our results revealed the novel roles for EMVs that could more effectively modulate vascular and brain aging than EEXs by regulating ECs functions through miR-17-5p/PI3K/Akt pathway, and also suggested that EMVs and EMV-miR-17-5p represent promising biomarkers and therapeutic targets for vascular aging.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"3 6","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145626591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell RNA sequencing reveals the niche transitions in the non-small cell lung cancer microenvironment mediated by piR-hsa-164586 during the invasive and metastatic processes","authors":"Yan Tang,&nbsp;Xiaomin Wang,&nbsp;Yanli Li,&nbsp;Kaibin Liu,&nbsp;Anjing Gong,&nbsp;Xin Ma,&nbsp;Jian Yuan,&nbsp;Yanhan Dong,&nbsp;Xiao Xu,&nbsp;Dan Han,&nbsp;Liang Zhang,&nbsp;Junlin Lv,&nbsp;Yiwei Xu,&nbsp;Zhen Shang,&nbsp;Nailong Pan,&nbsp;Xiaoying Kong,&nbsp;Wenhua Xu","doi":"10.1002/inmd.70070","DOIUrl":"https://doi.org/10.1002/inmd.70070","url":null,"abstract":"<p>Screening of tumor-specific markers and targeted therapy has achieved significant clinical efficacy in recent years. PIWI-interacting RNAs (piRNAs), which are crucial in the progression of tumors, are anticipated to be significant targets for the research and development of tumor molecular drugs aimed at tumors. This study corroborated the role of piR-hsa-164586 in enhancing the invasion and metastasis of non-small cell lung cancer (NSCLC) cells, as previously observed with its unusually high expression in NSCLC tissue and serum extracellular vesicles, through both in vitro and in vivo experiments. Single-cell RNA sequencing (scRNA-seq) combined with other molecular techniques revealed that the overexpression of piR-hsa-164586 regulates the tumor niche of NSCLC by upregulating mesenchymal subtypes of tumor epithelial cells. Furthermore, piR-hsa-164586 is involved in regulating PI3K-AKT pathway and epithelial–mesenchymal transition, thereby accelerating the invasion and metastasis of NSCLC cells. MYH9 (a non-PIWI protein) could bind to piR-hsa-164586 and the knockdown of piR-hsa-164586 or MYH9 links to better NSCLC prognoses. In conclusion, this work not only revealed the specific regulatory mechanism of piR-hsa-164586 as a tumor marker of NSCLC, but also provided support for developing molecular targeted therapies for NSCLC.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"3 6","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a specific marker panel for human mesenchymal stem cell-derived small extracellular vesicles","authors":"Lei Luo,&nbsp;Zhengsheng Chen,&nbsp;Ji Yuan,&nbsp;Xin Niu,&nbsp;Jiashuo Liu,&nbsp;Chao Gu,&nbsp;Hong Xu,&nbsp;Chuncui Jia,&nbsp;Tao Na,&nbsp;Shufang Meng,&nbsp;Yang Wang,&nbsp;Haiyan Li,&nbsp;Qing Li","doi":"10.1002/inmd.70065","DOIUrl":"https://doi.org/10.1002/inmd.70065","url":null,"abstract":"<p>Human mesenchymal stem cell-derived small extracellular vesicles (MSC-sEVs) have demonstrated significant immunomodulatory and pro-regenerative potentials. However, the lack of specific markers to define MSC-sEVs presents a major challenge for their clinical application. Here, the proteomic datasets of MSC-sEVs from three cell sources were synchronously analyzed, and several surface antigens commonly found on MSCs were selected as candidate markers due to their high abundances in MSC-sEVs. Next, MSC-sEVs from three cell sources (adipose tissue, umbilical cord, and induced pluripotent stem cells) were stained with fluorescein-conjugated antibodies and analyzed by NanoFCM at single-vesicle resolution. The positive rates of CD13, CD29, and CD90 all exceeded 60% across sEVs derived from three MSCs sources, whereas other candidates generally exhibited lower positive rates. The high positive rates of them were further verified in MSC-sEVs purified via other methods. Moreover, high-resolution microscopy, as an orthogonal method, visually validated their high presence in MSC-sEVs. Meanwhile, none of the non-MSC-sEVs showed concurrent positive rates for CD13, CD29, and CD90 exceeding 40%, suggesting that this marker panel (with a positive rate threshold of 50% for all three markers) could specifically distinguish MSC-sEVs from non-MSC-sEVs. Finally, the positive rates of this panel of markers were assessed in sEVs derived from MSCs at successive passages. The results revealed a progressive diminution of the positive rates of CD29 and CD90 in the sEVs secreted by MSCs with successive passages, accompanied by a reduction in the pro-proliferative activity of these sEVs. Taken together, we have identified a specific and quantifiable marker panel for MSC-sEVs characterization, which facilitates the development of standardized assays for defining MSC-sEVs and ultimately accelerates the clinical translation of MSC-sEVs.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"3 6","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mimicking DOUGONG brackets orchestrate regulating inflammation and mechanical stimuli for osteochondral regeneration using 3D printing","authors":"Bo Li,&nbsp;Yaqi Zhang,&nbsp;Xuerui Zhou,&nbsp;Weiguo Wang,&nbsp;Fan Yang,&nbsp;Qiushi Wei,&nbsp;Leilei Chen,&nbsp;Ziqi Li,&nbsp;Mincong He,&nbsp;Xiaoming He,&nbsp;Shengting Chai,&nbsp;Xiuhong Cao,&nbsp;Rui Wang,&nbsp;Tongmeng Jiang,&nbsp;Chen Wang,&nbsp;Wei He","doi":"10.1002/inmd.70068","DOIUrl":"10.1002/inmd.70068","url":null,"abstract":"<p>The microstructure of scaffolds is essential for enhancing cell-extracellular matrix interactions, which are critical for osteochondral regeneration. This study investigates the innovative use of 3D printing to mimic DOUGONG brackets to achieve this goal. Composite scaffolds of β-tricalcium phosphate, akermanite (AKer), and strontium silicate (SrSiO₃) at 1%, 5%, and 10% concentrations were fabricated via 3D printing. The osteogenic differentiation effects on bone marrow mesenchymal stromal cells and chondrogenic promotion effects on chondrocytes of different scaffolds were analyzed in vitro. An osteochondral defect model was created in SD rat knee joints, and various scaffold materials were implanted to evaluate their repair and anti-inflammatory effects. Characterization showed that SrSiO₃ composite scaffolds had uniform pore structures and high porosity with improved mechanical properties and sustained strontium ion release capacities. The Aker/Sr5% scaffold significantly enhanced cell viability and proliferation, inhibited apoptosis, and upregulated osteogenic markers (RUNX2, COL1A1, OCN, OPN) while downregulating inflammatory factors (IL-6 and TNF-α). This scaffold also improved the chondrocyte phenotype as indicated by increased chondrogenic markers (COL2A1 and SOX9). Additionally, it excelled in bone volume, bone mineral density, and tissue repair scores. The DOUGONG-inspired AKer/SrSiO₃ composite scaffold promotes osteochondral regeneration by enhancing osteogenesis via the PI3K/Akt signaling pathway and cytokine-cytokine receptor interaction, while simultaneously facilitating cartilage repair by promoting chondral phenotypes. This work provides a theoretical basis and new strategies for the clinical transformation of osteochondral repair.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"4 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146139685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoengineered neutrophils integrated with magnetic nanoparticles for T2-weighted magnetic resonance imaging-guided enhanced magnetic hyperthermia therapy of pancreatic tumor","authors":"Muladili Mutailipu,&nbsp;Ran Cui,&nbsp;Tianxiao Mei,&nbsp;Zinan Zhao,&nbsp;Yongkun Wang,&nbsp;Chunxiu Dong,&nbsp;Bingdi Chen,&nbsp;Yihui Hu,&nbsp;Wenjun Le,&nbsp;Bo Chen","doi":"10.1002/inmd.70061","DOIUrl":"10.1002/inmd.70061","url":null,"abstract":"<p>In recent years, magnetic hyperthermia therapy (MHT), recognized as a clinically promising anticancer treatment, has focused on enhancing therapeutic outcomes in tumor management, such as optimizing magnetic nanoparticle accumulation within tumor microenvironments and developing synergistic combination approaches with other therapies. Herein, we developed nanoengineered neutrophils (NE) by loading magnetic nanoparticles (FPP) onto healthy-derived NE, designated as FPPN. The combination of FPP's pronounced T₂-weighted magnetic resonance imaging (MRI) and superior magnetothermal efficiency with the high tumor-targeting and cancer-killing activity of NE successfully achieved the synergistic implementation of MHT and NE. Under the guidance of T₂-weighted MRI, the accumulation of FPPN within the tumor site demonstrated a significant enhancement compared with free FPP. Under exposure to alternating magnetic fields, the thermal energy produced by FPP and FPPN could ablate tumor cells directly. Intriguingly, mitochondria within NE exhibit significant reactive oxygen species (ROS) under magnetothermal activation, thereby enhancing therapeutic efficacy and enabling synergistic tumor treatment through the combination of MHT and NE-mediated ROS. This therapeutic strategy not only highlighted the significant potential of nanoengineered NE for highly efficient MHT but also established a novel therapeutic platform for precision-targeted treatment of deep-seated tumors.</p>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"4 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146139681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}