Interdisciplinary Medicine最新文献_第9页

Advances and challenges in point-of-care testing for pathogenic bacteria in battlefield injuries 战场创伤致病菌即时检测的进展与挑战

IF 13.6

Interdisciplinary Medicine Pub Date : 2026-01-06 DOI: 10.1002/inmd.70071

Junjie Fan, Shuai Liu, Dan Luo, Hengjun Zhang, Xianling Dai, Hanqing Xu, Man Shen, Yingchun Huang, Yupeng Long, Ming Chen, Qiaofeng Li, Jing Bao

{"title":"Advances and challenges in point-of-care testing for pathogenic bacteria in battlefield injuries","authors":"Junjie Fan, Shuai Liu, Dan Luo, Hengjun Zhang, Xianling Dai, Hanqing Xu, Man Shen, Yingchun Huang, Yupeng Long, Ming Chen, Qiaofeng Li, Jing Bao","doi":"10.1002/inmd.70071","DOIUrl":"https://doi.org/10.1002/inmd.70071","url":null,"abstract":"Infections are a leading contributor to delayed mortality in patients with combat-related trauma. Thus, the real-time, accurate, and rapid detection of invasive pathogens is essential for optimizing wound management and accelerating recovery. Recent innovations in point-of-care testing (POCT) biosensors, which are characterized by enhanced portability and multifunctionality, offer promising solutions for onsite diagnostics without relying on centralized laboratories. These technologies are particularly effective for the minimally invasive monitoring of real-time biophysical or biochemical signals at wound sites. This review explores the species and distribution of bacteria associated with combat-related injuries. Thereafter, we summarize recent advancements in optical and other biosensors (electrochemical, piezoelectric, and thermal) used in bacterial POCT. Key integration strategies are highlighted, including biosensor coupling with microdevices (e.g., microfluidics and microarrays), smart systems (e.g., smartphone readouts and artificial intelligence-based interpretation), and the Internet of Things. We also address the current challenges in biosensor fabrication and outline the expected future directions for biosensing. This review aims to provide a useful reference for the development of POCT biosensors for bacterial detection in combat and trauma care settings.","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"4 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70071","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146139549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A clustered regularly interspaced palindromic repeat-Cas12a-based assay for rapid dengue virus monitoring in mosquito and clinical samples facilitating global collaborative surveillance and risk assessment to block dengue spreading 一种在蚊子和临床样本中快速监测登革热病毒的定期间隔回文重复cas12聚类分析，促进全球合作监测和风险评估，以阻止登革热传播

IF 13.6

Interdisciplinary Medicine Pub Date : 2026-01-06 DOI: 10.1002/inmd.70074

Xiang Guo, Minling Hu, Ying Lu, Xiaohua Liu, Haiyang Chen, Xiaoqing Zhang, Liu Ge, Ziyao Li, Yuji Wang, Qing He, Shu Zeng, Wenwen Ren, Lingzhai Zhao, Zhiqiang Peng, Wushen Chen, Chunmei Wang, Qingming Kong, Shaohong Lu, Fuchun Zhang, Lei Luo, Hua Li, Xiao-Guang Chen, Xiaohong Zhou

{"title":"A clustered regularly interspaced palindromic repeat-Cas12a-based assay for rapid dengue virus monitoring in mosquito and clinical samples facilitating global collaborative surveillance and risk assessment to block dengue spreading","authors":"Xiang Guo, Minling Hu, Ying Lu, Xiaohua Liu, Haiyang Chen, Xiaoqing Zhang, Liu Ge, Ziyao Li, Yuji Wang, Qing He, Shu Zeng, Wenwen Ren, Lingzhai Zhao, Zhiqiang Peng, Wushen Chen, Chunmei Wang, Qingming Kong, Shaohong Lu, Fuchun Zhang, Lei Luo, Hua Li, Xiao-Guang Chen, Xiaohong Zhou","doi":"10.1002/inmd.70074","DOIUrl":"10.1002/inmd.70074","url":null,"abstract":"Dengue fever, an acute Aedes-borne infectious disease caused by the dengue virus (DENV), is a substantial global health challenge, necessitating the development of swift and effective surveillance methodologies. We have developed a Recombinase Aided Amplification (RAA)–Cas12a assay that exhibits superior analytical sensitivity compared with real-time PCR while maintaining comparable specificity. In clinical specimen testing, the assay demonstrated a sensitivity of 92.3% and a specificity of 100%, using a signal-to-noise ratio of ≥2.0 as the positivity threshold. Furthermore, the RAA–Cas12a assay displayed remarkable sensitivity in detecting DENV in both adult and larval mosquitoes, with field data from mosquito surveillance aligning closely with the emergence of local dengue cases. Characterized by its simplicity, rapidity, and precision, the RAA–Cas12a approach emerges as a highly promising technology for monitoring virus carriage in field-collected mosquitoes, thereby functioning as both an early warning system and a crucial indicator for effective dengue outbreak management. The practicality and cost-effectiveness of this methodology provide a robust foundation for the establishment of an efficient global collaborative surveillance and risk assessment platform to mitigate the rapid spread of dengue.","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"4 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70074","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146139551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicle-based vaccines and immunotherapeutics for treatment of cancer 用于癌症治疗的细胞外囊泡疫苗和免疫疗法

IF 13.6

Interdisciplinary Medicine Pub Date : 2026-01-06 DOI: 10.1002/inmd.70084

Lejia Tan, Haonan Xing, Fang Fang, Yuanyu Huang, Mei Lu

{"title":"Extracellular vesicle-based vaccines and immunotherapeutics for treatment of cancer","authors":"Lejia Tan, Haonan Xing, Fang Fang, Yuanyu Huang, Mei Lu","doi":"10.1002/inmd.70084","DOIUrl":"https://doi.org/10.1002/inmd.70084","url":null,"abstract":"In recent years, cancer immunotherapy has gained substantial momentum, particularly in the field of vaccines and immunotherapeutics. Among these advancements, extracellular vesicles (EVs) have emerged as a promising cell-free vaccine system due to their unique capacity to display diverse antigens and immune-stimulatory molecules. Additionally, their excellent biocompatibility, ability to permeate across biological barriers, and targeting properties make EVs a promising platform for delivery of various therapeutics for cancer immunotherapy, such as RNAs, proteins, lipids, and chemotherapeutic agents. To date, EV-based vaccines and immunotherapeutics have demonstrated remarkable efficacy in combating various types of cancers. Herein, we endeavor to give a comprehensive summary of EV-based vaccines and immunotherapeutics for the treatment of cancers, delving into their advantages and challenges. Furthermore, emphasis was given on offering insight into the design rationale of each platform. Lastly, we recapitulate the recent preclinical and clinical application progress of EV-based vaccines and immunotherapeutics, highlighting their pivotal role in cancer immunotherapy.","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"4 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146139582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A clustered regularly interspaced palindromic repeat-Cas12a-based assay for rapid dengue virus monitoring in mosquito and clinical samples facilitating global collaborative surveillance and risk assessment to block dengue spreading 一种在蚊子和临床样本中快速监测登革热病毒的定期间隔回文重复cas12聚类分析，促进全球合作监测和风险评估，以阻止登革热传播

IF 13.6

Interdisciplinary Medicine Pub Date : 2026-01-06 DOI: 10.1002/inmd.70074

Xiang Guo, Minling Hu, Ying Lu, Xiaohua Liu, Haiyang Chen, Xiaoqing Zhang, Liu Ge, Ziyao Li, Yuji Wang, Qing He, Shu Zeng, Wenwen Ren, Lingzhai Zhao, Zhiqiang Peng, Wushen Chen, Chunmei Wang, Qingming Kong, Shaohong Lu, Fuchun Zhang, Lei Luo, Hua Li, Xiao-Guang Chen, Xiaohong Zhou

{"title":"A clustered regularly interspaced palindromic repeat-Cas12a-based assay for rapid dengue virus monitoring in mosquito and clinical samples facilitating global collaborative surveillance and risk assessment to block dengue spreading","authors":"Xiang Guo, Minling Hu, Ying Lu, Xiaohua Liu, Haiyang Chen, Xiaoqing Zhang, Liu Ge, Ziyao Li, Yuji Wang, Qing He, Shu Zeng, Wenwen Ren, Lingzhai Zhao, Zhiqiang Peng, Wushen Chen, Chunmei Wang, Qingming Kong, Shaohong Lu, Fuchun Zhang, Lei Luo, Hua Li, Xiao-Guang Chen, Xiaohong Zhou","doi":"10.1002/inmd.70074","DOIUrl":"10.1002/inmd.70074","url":null,"abstract":"Dengue fever, an acute Aedes-borne infectious disease caused by the dengue virus (DENV), is a substantial global health challenge, necessitating the development of swift and effective surveillance methodologies. We have developed a Recombinase Aided Amplification (RAA)–Cas12a assay that exhibits superior analytical sensitivity compared with real-time PCR while maintaining comparable specificity. In clinical specimen testing, the assay demonstrated a sensitivity of 92.3% and a specificity of 100%, using a signal-to-noise ratio of ≥2.0 as the positivity threshold. Furthermore, the RAA–Cas12a assay displayed remarkable sensitivity in detecting DENV in both adult and larval mosquitoes, with field data from mosquito surveillance aligning closely with the emergence of local dengue cases. Characterized by its simplicity, rapidity, and precision, the RAA–Cas12a approach emerges as a highly promising technology for monitoring virus carriage in field-collected mosquitoes, thereby functioning as both an early warning system and a crucial indicator for effective dengue outbreak management. The practicality and cost-effectiveness of this methodology provide a robust foundation for the establishment of an efficient global collaborative surveillance and risk assessment platform to mitigate the rapid spread of dengue.","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"4 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70074","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146139539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aggregation-induced emission luminogens in in vitro diagnostics: Advancing biomarker detection in body fluids 体外诊断中的聚集诱导发光原：推进体液中生物标志物的检测

IF 13.6

Interdisciplinary Medicine Pub Date : 2026-01-06 DOI: 10.1002/inmd.70066

Qi Liu, Xirui Chen, Kenian Wang, Yifan Wu, Xiangcai Zou, Ying Li

{"title":"Aggregation-induced emission luminogens in in vitro diagnostics: Advancing biomarker detection in body fluids","authors":"Qi Liu, Xirui Chen, Kenian Wang, Yifan Wu, Xiangcai Zou, Ying Li","doi":"10.1002/inmd.70066","DOIUrl":"10.1002/inmd.70066","url":null,"abstract":"Rapid, sensitive, and accurate detection of ingredients in body fluids is crucial for monitoring health status and diagnosing diseases. Fluorescent biosensors have emerged as a user-friendly tool for biomarker detection, because of their high sensitivity, rapid response, and adaptability. Among the various signal probes used in fluorescent biosensors, aggregation-induced emission (AIE) luminogens (AIEgens) stand out as a highly promising candidate owing to their unique property of enhanced emission in the aggregated or solid state. This review outlines the sensing mechanisms and design strategies of AIE probes for constructing AIE-based fluorescent biosensors. It provides a comprehensive overview of recent advancements in AIE probes for detecting various ingredients in body fluids, including blood, urine, saliva, throat swabs, and sweat. The efficacy of AIEgen-based fluorescent biosensors in these applications is highlighted, focusing on the design of probes, response mechanisms, and luminescence principles. Finally, the current challenges of AIEgen-based biosensors and future research perspectives are discussed, aiming to inspire the development of AIE probes and improve the sensitivity and accessibility of AIEgen-based fluorescent biosensors.","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"4 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146139552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicle-based vaccines and immunotherapeutics for treatment of cancer 用于癌症治疗的细胞外囊泡疫苗和免疫疗法

IF 13.6

Interdisciplinary Medicine Pub Date : 2026-01-06 DOI: 10.1002/inmd.70084

Lejia Tan, Haonan Xing, Fang Fang, Yuanyu Huang, Mei Lu

{"title":"Extracellular vesicle-based vaccines and immunotherapeutics for treatment of cancer","authors":"Lejia Tan, Haonan Xing, Fang Fang, Yuanyu Huang, Mei Lu","doi":"10.1002/inmd.70084","DOIUrl":"10.1002/inmd.70084","url":null,"abstract":"In recent years, cancer immunotherapy has gained substantial momentum, particularly in the field of vaccines and immunotherapeutics. Among these advancements, extracellular vesicles (EVs) have emerged as a promising cell-free vaccine system due to their unique capacity to display diverse antigens and immune-stimulatory molecules. Additionally, their excellent biocompatibility, ability to permeate across biological barriers, and targeting properties make EVs a promising platform for delivery of various therapeutics for cancer immunotherapy, such as RNAs, proteins, lipids, and chemotherapeutic agents. To date, EV-based vaccines and immunotherapeutics have demonstrated remarkable efficacy in combating various types of cancers. Herein, we endeavor to give a comprehensive summary of EV-based vaccines and immunotherapeutics for the treatment of cancers, delving into their advantages and challenges. Furthermore, emphasis was given on offering insight into the design rationale of each platform. Lastly, we recapitulate the recent preclinical and clinical application progress of EV-based vaccines and immunotherapeutics, highlighting their pivotal role in cancer immunotherapy.","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"4 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146139550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 13.6

Interdisciplinary Medicine Pub Date : 2026-01-06

引用次数: 0

Aggregation-induced emission luminogens in in vitro diagnostics: Advancing biomarker detection in body fluids 体外诊断中的聚集诱导发光原：推进体液中生物标志物的检测

IF 13.6

Interdisciplinary Medicine Pub Date : 2026-01-06 DOI: 10.1002/inmd.70066

Qi Liu, Xirui Chen, Kenian Wang, Yifan Wu, Xiangcai Zou, Ying Li

{"title":"Aggregation-induced emission luminogens in in vitro diagnostics: Advancing biomarker detection in body fluids","authors":"Qi Liu, Xirui Chen, Kenian Wang, Yifan Wu, Xiangcai Zou, Ying Li","doi":"10.1002/inmd.70066","DOIUrl":"https://doi.org/10.1002/inmd.70066","url":null,"abstract":"Rapid, sensitive, and accurate detection of ingredients in body fluids is crucial for monitoring health status and diagnosing diseases. Fluorescent biosensors have emerged as a user-friendly tool for biomarker detection, because of their high sensitivity, rapid response, and adaptability. Among the various signal probes used in fluorescent biosensors, aggregation-induced emission (AIE) luminogens (AIEgens) stand out as a highly promising candidate owing to their unique property of enhanced emission in the aggregated or solid state. This review outlines the sensing mechanisms and design strategies of AIE probes for constructing AIE-based fluorescent biosensors. It provides a comprehensive overview of recent advancements in AIE probes for detecting various ingredients in body fluids, including blood, urine, saliva, throat swabs, and sweat. The efficacy of AIEgen-based fluorescent biosensors in these applications is highlighted, focusing on the design of probes, response mechanisms, and luminescence principles. Finally, the current challenges of AIEgen-based biosensors and future research perspectives are discussed, aiming to inspire the development of AIE probes and improve the sensitivity and accessibility of AIEgen-based fluorescent biosensors.","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"4 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146139583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inflammatory bowel disease and metabolic reprogramming: From pathological mechanisms to targeted interventions 炎症性肠病和代谢重编程：从病理机制到针对性干预

IF 13.6

Interdisciplinary Medicine Pub Date : 2026-01-06 DOI: 10.1002/inmd.70083

Zemin Tian, Yinde Huang, Ying Wang, Chenyu Zhao, Zhaokai Zhou, Hua Yang, Yuan Qiu

{"title":"Inflammatory bowel disease and metabolic reprogramming: From pathological mechanisms to targeted interventions","authors":"Zemin Tian, Yinde Huang, Ying Wang, Chenyu Zhao, Zhaokai Zhou, Hua Yang, Yuan Qiu","doi":"10.1002/inmd.70083","DOIUrl":"10.1002/inmd.70083","url":null,"abstract":"Inflammatory bowel disease (IBD) comprises Crohn's disease (CD) and ulcerative colitis and arises from the convergence of genetic susceptibility, environmental exposures, immune dysregulation, and gut microbiome perturbations. Metabolic reprogramming has emerged as a central feature of IBD pathogenesis. Immune and epithelial cells in IBD show coordinated disturbances in glycolysis, fatty-acid metabolism, and oxidative phosphorylation, which shape macrophage and T-cell polarization and weaken epithelial barrier integrity. Microbiota-derived metabolites, including short-chain fatty acids (SCFAs), bile acids, and tryptophan derivatives, act as key immunometabolic signals, with SCFAs promoting regulatory programs and barrier repair, whereas disordered bile-acid pools and dysregulated receptor signaling can drive inflammation and disrupt the barrier. These insights motivate metabolic interventions that complement immunotherapy, including modulation of the microbiota, supplementation or engineered delivery of specific metabolites, and targeting of metabolic pathways (AMPK, mTOR) and receptors (FXR, TGR5, AhR). Early clinical studies indicate potential benefit in selected patient subsets, although effect sizes are variable, and translation remains limited by disease heterogeneity, context-dependent metabolite effects, suboptimal delivery to inflamed segments, and the absence of predictive biomarkers. This review synthesizes recent advances in IBD immunometabolism, integrates evidence across cells, pathways, and microbial metabolites, and outlines actionable opportunities for development, including patient stratification, target-engagement readouts, and rigorously designed trials with endpoints such as mucosal healing, corticosteroid-free remission, and relapse reduction.","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"4 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146136067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a specific marker panel for human mesenchymal stem cell-derived small extracellular vesicles (6/2025) 人间充质干细胞来源的细胞外小泡特异性标记板的鉴定（6/2025）

IF 13.6

Interdisciplinary Medicine Pub Date : 2025-11-28 DOI: 10.1002/inmd.70080

Lei Luo, Zhengsheng Chen, Ji Yuan, Xin Niu, Jiashuo Liu, Chao Gu, Hong Xu, Chuncui Jia, Tao Na, Shufang Meng, Yang Wang, Haiyan Li, Qing Li

{"title":"Identification of a specific marker panel for human mesenchymal stem cell-derived small extracellular vesicles (6/2025)","authors":"Lei Luo, Zhengsheng Chen, Ji Yuan, Xin Niu, Jiashuo Liu, Chao Gu, Hong Xu, Chuncui Jia, Tao Na, Shufang Meng, Yang Wang, Haiyan Li, Qing Li","doi":"10.1002/inmd.70080","DOIUrl":"https://doi.org/10.1002/inmd.70080","url":null,"abstract":"This cover art draws inspiration from the Chinese mythological tale of distinguishing the true from the false Monkey King, symbolizing our study’s identification of a specific marker panel (CD13, CD29, and CD90) that distinguishes mesenchymal stem cell-derived small extracellular vesicles (MSC-sEVs) from non-MSC-sEVs. The true Monkey King represents MSC-sEVs, bearing the three specific markers alongside the universal EV marker tetraspanin, while the impostor monkey symbolizes non-MSC-sEVs, displaying only tetraspanin. The mythical “demon-revealing mirror” reflects the single-vesicle resolution analysis technology, enabling precise discrimination of MSC-sEVs by detecting the positive rates of CD13, CD29, and CD90.Our study is the first to identify CD13, CD29, and CD90 as a specific marker panel for MSC-sEVs from three cell sources (adipose tissue, umbilical cord and iPSCs). By quantitatively analyzing these markers at single-vesicle resolution, we achieve breakthrough discrimination between MSC-sEVs and non-MSC-sEVs, providing a critical tool for standardized MSC-sEV characterization. The allegory of discerning the true Monkey King aptly captures the clinical and research value of our findings. This marker panel contributes to the establishment of quantifiable, reproducible, and standardized assays for defining MSC-sEVs preparations, facilitating data comparison across studies, which holds practical significance for advancing the clinical application of MSC-sEVs. Designed by Jialin Guo, Guangzhou Academy of Fine Arts. Supported by Rongrong Cao, Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture>\u0000 </div>\u0000 </figure>","PeriodicalId":100686,"journal":{"name":"Interdisciplinary Medicine","volume":"3 6","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/inmd.70080","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145619337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0