Green Carbon最新文献_第2页

Improving Chlorella protein production under heterotrophic high cell density fed-batch cultivation with a two-stage nitrogen nutrient supply strategy 采用两阶段氮营养供给策略提高异养高密度分批补料培养小球藻蛋白质产量

Green Carbon Pub Date : 2025-06-01 DOI: 10.1016/j.greenca.2024.10.002

Baofeng Chen , Ren Gong , Zhixian Qiao , Jun Men , Lu Tan , Shilin Tian , Hu Jin

{"title":"Improving Chlorella protein production under heterotrophic high cell density fed-batch cultivation with a two-stage nitrogen nutrient supply strategy","authors":"Baofeng Chen , Ren Gong , Zhixian Qiao , Jun Men , Lu Tan , Shilin Tian , Hu Jin","doi":"10.1016/j.greenca.2024.10.002","DOIUrl":"10.1016/j.greenca.2024.10.002","url":null,"abstract":"<div><div>Heterotrophic cultivation has been proved to be an effective and industrially scalable route for the economical production of <em>Chlorella</em> biomass. Nevertheless, the low protein content of heterotrophic <em>Chlorella</em> biomass hinders its utilization as a superior protein source. In this study, the influences of various C/N ratios on cell growth and protein production were evaluated for a previously isolated protein-rich alga <em>Chlorella sorokiniana</em> CMBB276. The results indicated that a high C/N ratio was favorable for cell growth, whereas a low C/N ratio was advantageous for protein synthesis. A proposed two-stage nitrogen supply strategy by shifting the C/N ratio from 18 to 6 was found to be capable of simultaneously improve the protein content and yield of <em>C. sorokiniana</em> CMBB276, achieving the maximum protein content of 58.6% of dry cell weight (DCW) and a final protein yield of 87.0 g L<sup>−1</sup>, respectively. We further verified that the fast accumulated ammonium in <em>C. sorokiniana</em> CMBB276 cultivated with the switched C/N ratio contributed to the remarkable enhancement of protein content, while concurrently causing cytotoxicity. Moreover, under high ammonium stress, while maintaining a high protein content, <em>C. sorokiniana</em> CMBB276 persisted in growing with a decreased level of cellular reactive oxygen species (ROS) and cell membrane damage through exogenous addition of acetate, which lays the foundation for further improvement of protein production by applying the two-stage nitrogen supply strategy in combination with acetate addition.</div></div>","PeriodicalId":100595,"journal":{"name":"Green Carbon","volume":"3 2","pages":"Pages 208-217"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144492015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Closed-loop transfer brightens up artificial intelligence for chemistry 闭环转移点亮了化学领域的人工智能

Green Carbon Pub Date : 2025-06-01 DOI: 10.1016/j.greenca.2024.12.001

Qipeng Li , Mengyun Chen , Tiankai Zhang

引用次数: 0

Engineering microbiomes to enhance macroalgal health, biomass yield, and carbon sequestration 工程微生物组，以提高大藻的健康，生物量产量和碳固存

Green Carbon Pub Date : 2025-03-01 DOI: 10.1016/j.greenca.2024.11.001

Shailesh Nair , Zenghu Zhang , Xiaojie Wang , Bo Zhang , Nianzhi Jiao , Yongyu Zhang

{"title":"Engineering microbiomes to enhance macroalgal health, biomass yield, and carbon sequestration","authors":"Shailesh Nair , Zenghu Zhang , Xiaojie Wang , Bo Zhang , Nianzhi Jiao , Yongyu Zhang","doi":"10.1016/j.greenca.2024.11.001","DOIUrl":"10.1016/j.greenca.2024.11.001","url":null,"abstract":"<div><div>Macroalgae farming not only holds significant economic value but also contributes substantially to carbon sequestration, and therefore has gained intensified attention globally under climate change scenarios. However, its sustainability is increasingly threatened by anthropogenic and environmental changes. The health and resilience of macroalgae are intrinsically linked to their associated microbiomes, offering an untapped opportunity to enhance macroalgal farming through microbiome manipulation. In this review, we have summarized the current understanding of macroalgal microbiomes, highlighting critically underexplored microbial components, such as overlooked taxa, host specificity, and the environmental factors influencing microbiome composition, which hinder the development of effective microbiome engineering strategies. We critically evaluate existing microbiome manipulation approaches and their applications in enhancing macroalgal growth, resilience, carbon fixation, and biomass yield and assess their potential for improving macroalgal carbon sequestration. Finally, we propose a holistic framework that integrates multi-omics and metabolic modeling, microbial functional and environmental compatibility, high-throughput rapid isolation, and <em>in vivo</em> validation to bridge critical knowledge gaps and unlock the full potential of macroalgal microbiome engineering for sustainable, large-scale macroalgal farming.</div></div>","PeriodicalId":100595,"journal":{"name":"Green Carbon","volume":"3 1","pages":"Pages 63-73"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in the application of in situ X-ray diffraction techniques to characterize phase transitions in Fischer–Tropsch synthesis catalysts 原位x射线衍射技术表征费托合成催化剂相变的最新进展

Green Carbon Pub Date : 2025-03-01 DOI: 10.1016/j.greenca.2024.09.009

Fan Bo , Kangzhou Wang , Jie Liang , Tiejian Zhao , Jie Wang , Yurong He , Xiaojiao Yang , Jianli Zhang , Yongjun Jiang , Xiaojing Yong , Wei Zhang , Xinhua Gao

{"title":"Recent advances in the application of in situ X-ray diffraction techniques to characterize phase transitions in Fischer–Tropsch synthesis catalysts","authors":"Fan Bo , Kangzhou Wang , Jie Liang , Tiejian Zhao , Jie Wang , Yurong He , Xiaojiao Yang , Jianli Zhang , Yongjun Jiang , Xiaojing Yong , Wei Zhang , Xinhua Gao","doi":"10.1016/j.greenca.2024.09.009","DOIUrl":"10.1016/j.greenca.2024.09.009","url":null,"abstract":"<div><div>Fischer–Tropsch synthesis (FTS), an important route for the conversion of syngas into high-value-added chemicals, often relies on Fe- and Co-based catalysts. Catalyst performance has been improved by promoters, supports, and optimization of the activation process; however, in-depth studies on the evolution of the phase during catalyst activation, reaction, and deactivation are still lacking. <em>In situ</em> X-ray diffraction (XRD) effectively reveals the phase evolution of catalytic materials in real time. In this review, the use of <em>in situ</em> XRD to elucidate the influence of activation mode, promoters, and supports on the phase evolution and performance of Fe- and Co-based catalysts is examined. The challenges and opportunities in studying the phase evolution of FTS catalysts using <em>in situ</em> XRD techniques are summarized and discussed, and theoretical guidance for the design of FTS catalysts is provided in order to promote their development.</div></div>","PeriodicalId":100595,"journal":{"name":"Green Carbon","volume":"3 1","pages":"Pages 22-35"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MADS-box transcription factors as green-revolution regulators for plant longevity and biomass production MADS-box转录因子作为植物寿命和生物量生产的绿色革命调控因子

Green Carbon Pub Date : 2025-03-01 DOI: 10.1016/j.greenca.2024.09.007

Yixiang Wang , Jingyuan Liu , Xifeng Ren , Chunxiang Fu , Liangcai Peng , Yanting Wang

引用次数: 0

Nitrogen-functionalized modulation of iron nanoparticles promotes selective hydrogenation of carbon dioxide 铁纳米颗粒的氮功能化调制促进了二氧化碳的选择性氢化

Green Carbon Pub Date : 2025-03-01 DOI: 10.1016/j.greenca.2024.09.010

Xianbiao Wang , Jun Qian , Zixuan Lu , Jie Huang , Liru Zheng , Yong Jiang , Mengdie Cai , Yuxue Wei , Lisheng Guo , Song Sun

引用次数: 0

Enhancing perovskite/silicon tandem solar cells via nuclei engineering 通过核工程增强钙钛矿/硅串联太阳能电池

Green Carbon Pub Date : 2025-03-01 DOI: 10.1016/j.greenca.2024.09.006

Dongyang Li , Xinyu Jiang , Peter Müller-Buschbaum , Ruijie Ma , Gang Li

引用次数: 0

Rational design of H2 production sites for achieving photoconversion of CO2 with H2O into widely adjustable syngas and highly effective H2 evolution 合理设计产氢位点，实现CO2与H2O光转化为可广泛调节的合成气和高效的氢气析出

Green Carbon Pub Date : 2025-03-01 DOI: 10.1016/j.greenca.2024.07.008

Guoqiang Zhang , Zhiqi Wang , Diandian Shi , Guangbo Liu , Tao He , Jingli Wu , Jinzhi Zhang , Jinhu Wu

{"title":"Rational design of H2 production sites for achieving photoconversion of CO2 with H2O into widely adjustable syngas and highly effective H2 evolution","authors":"Guoqiang Zhang , Zhiqi Wang , Diandian Shi , Guangbo Liu , Tao He , Jingli Wu , Jinzhi Zhang , Jinhu Wu","doi":"10.1016/j.greenca.2024.07.008","DOIUrl":"10.1016/j.greenca.2024.07.008","url":null,"abstract":"<div><div>The photoconversion of CO<sub>2</sub> with H<sub>2</sub>O into widely tunable syngas (CO and H<sub>2</sub>) or pure H<sub>2</sub> production is regarded as a promising strategy to mitigate escalating energy shortages and climate change. Herein, anchoring the H<sub>2</sub> production sites onto the surface of CdIn<sub>2</sub>S<sub>4</sub> (CIS) with a nanoscale hollow sphere allows for the photoconversion of CO<sub>2</sub> into syngas and water splitting to H<sub>2</sub>. The CO/H<sub>2</sub> ratio can be realized in a remarkably wide range from 1:0.38 to 1:3.76. The optimized CIS/Co-PBA/NaY-5 hybrid exhibits superior photocatalytic syngas evolution up to 1458.48 μmol·g<sup>−1</sup>·h<sup>−1</sup> (H<sub>2</sub>/CO, 1152.29/306.19 μmol·g<sup>−1</sup>·h<sup>−1</sup>), and the H<sub>2</sub> evolution rate increases by 431.70% compared with CIS. The CIS/Co-PBA/NaY-5 hybrid exhibited not only superior H<sub>2</sub> evolution but also recyclability. The experimental, energy-dispersive X-ray spectroscopy, and electron spin resonance results indicate that the Co sites serve as H<sub>2</sub> production sites and promote the H<sub>2</sub> evolution reaction. In addition, the construction of a p-n heterojunction with a special micromorphology is beneficial for the separation/transfer of carriers.</div></div>","PeriodicalId":100595,"journal":{"name":"Green Carbon","volume":"3 1","pages":"Pages 11-21"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in microalgae-driven carbon capture, utilization, and storage: Strain engineering through adaptive laboratory evolution and microbiome optimization 微藻驱动的碳捕获、利用和储存的最新进展：通过适应性实验室进化和微生物组优化的菌株工程

Green Carbon Pub Date : 2025-03-01 DOI: 10.1016/j.greenca.2024.10.001

Zhongshi He , Jing Wang , Yantao Li

{"title":"Recent advances in microalgae-driven carbon capture, utilization, and storage: Strain engineering through adaptive laboratory evolution and microbiome optimization","authors":"Zhongshi He , Jing Wang , Yantao Li","doi":"10.1016/j.greenca.2024.10.001","DOIUrl":"10.1016/j.greenca.2024.10.001","url":null,"abstract":"<div><div>The potential of microalgae as a biological resource for carbon capture, utilization, and storage (CCUS) has been extensively discussed. Although genetic engineering methods have been employed to improve microalgal phenotypes, they often face challenges related to public concerns regarding genetically modified organisms. By contrast, adaptive laboratory evolution (ALE) and microbiome optimization have emerged as promising non-genetic modification strategies, with notable success in bacterial models. In microalgae, ALE has been employed to improve resilience against varying environmental and stress factors and increase carbon capture efficiency, and for the production of valuable bioproducts through gradual accumulation of beneficial mutations following manual or automated selection. Furthermore, advancements in the understanding of microbial symbiotic relationships in the phycosphere have facilitated microbiome optimization in microalgal cultivation systems, significantly improving their functionality and productivity. In this study, we provide a comprehensive overview of the latest advancements in ALE and microbiome optimization of microalgae for CCUS across different carbon emission scenarios, including flue gas, biogas, wastewater, and landfill leachate. We further discuss the current challenges and future directions for the integration of ALE with microbiome optimization, focusing on the potential synergies of these methodologies. Overall, ALE and microbiome optimization are promising approaches to direct microalgae for environmental and industrial CCUS applications, thereby reducing global carbon emissions and addressing climate change challenges.</div></div>","PeriodicalId":100595,"journal":{"name":"Green Carbon","volume":"3 1","pages":"Pages 74-99"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alcohol pretreatment for depolymerization and fractionation of corn stalk 玉米秸秆醇预处理解聚分馏研究

Green Carbon Pub Date : 2025-03-01 DOI: 10.1016/j.greenca.2024.09.005

Lijuan Gao , Wentao Li , Yu Zhang , Meijing Wang , Wen Wang , Cuiyi Liang , Shiyou Xing , Wei Qi

{"title":"Alcohol pretreatment for depolymerization and fractionation of corn stalk","authors":"Lijuan Gao , Wentao Li , Yu Zhang , Meijing Wang , Wen Wang , Cuiyi Liang , Shiyou Xing , Wei Qi","doi":"10.1016/j.greenca.2024.09.005","DOIUrl":"10.1016/j.greenca.2024.09.005","url":null,"abstract":"<div><div>The development of an efficient pretreatment technology to depolymerize and fractionate lignocellulose into glucan, xylan, and lignin is crucial for lignocellulose biorefinery. In this study, alcohol pretreatments using methanol and pentanol were developed and compared. Based on the solubility of the two alcohols, the methanol and pentanol pretreatments are homogeneous and biphasic, respectively. Carbon flow analysis revealed that 1 kg of corn stalk (CS) yielded 111.9 g of lignin with the homogeneous pretreatment (lignin yield: 60.0%), while 149.8 g of lignin was obtained with the biphasic pretreatment (lignin yield: 80.3%). Biphasic pretreatment yielded the lignin with higher activity (more β-O-4 bond content). Additionally, 210.4 g and 267.0 g of glucose were obtained from the enzymatic hydrolysis of homogeneously and biphasically pretreated CS, respectively, where glucose yields were 53.0% and 67.2%, respectively. Fourier Transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) were employed to verify the component fractionation results. The Hansen solubility parameter and combined severity factor analysis were used to evaluate the effects of various factors on component fractionation. Nuclear magnetic resonance and mechanistic analyses were performed to explore the process of component fractionation. Overall, we discovered that biphasic pretreatment was significantly better than homogeneous pretreatment in component fractionation, including component recovery and lignin activity.</div></div>","PeriodicalId":100595,"journal":{"name":"Green Carbon","volume":"3 1","pages":"Pages 100-109"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0