Chemosensors最新文献

{"title":"Characteristics of Peanut Protein-Derived Carbon Dots and Their Application in Cell Imaging and Sensing of Metronidazole","authors":"Junyan Liao, Zhixiong Hu, Weinong Zhang, Yanpeng Zhang, Jiangrong Xiao, Shenglan Lei","doi":"10.3390/chemosensors13040151","DOIUrl":"https://doi.org/10.3390/chemosensors13040151","url":null,"abstract":"In this paper, peanut protein (PP) was used as the sole raw material for the preparation of fluorescent carbon dots (PP-CDs) by hydrothermal method. The PP-CDs exhibit good dispersibility, spherical-like shapes, and uniform sizes; the average particle size of the PP-CDs was 3.18 ± 0.17 nm. The Fourier transform infrared spectroscopy (FTIR) results show that the surface of PP-CDs is rich in hydrophilic groups such as hydroxyl, carboxyl and amide groups. The PP-CDs exhibit good fluorescence emission properties and excitation wavelength dependence, with the optimal excitation wavelength and emission wavelength at 348 nm and 452 nm, respectively. According to the fluorescence quenching effect of metronidazole (MTZ) and tinidazole (TDZ) on PP-CDs, a highly linear fluorescence sensor was established, with a concentration range of 0.10–60.0 µM, and the detection limits of MTZ and TDZ are 32.0 nM and 48.0 nM, respectively. The result of CCK-8 test and imaging of HepG-2 cells and onion epidermal cells reveal that PP-CDs have good membrane permeability, biocompatibility and imaging ability.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 4","pages":"151-151"},"PeriodicalIF":0.0,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2227-9040/13/4/151/pdf?version=1745212013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147918098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzyme Inhibition-Mediated Distance-Based Paper Biosensor for Organophosphate Pesticide Detection in Food Samples","authors":"Yulin Liu, Longzhan Dong, Qiognzheng Hu, Jingbo Chen, Mashooq Khan","doi":"10.3390/chemosensors13040147","DOIUrl":"https://doi.org/10.3390/chemosensors13040147","url":null,"abstract":"Organophosphate pesticides (OPs) enter the environment through various avenues, posing significant health risks. This highlights the need to monitor OPs in food and environmental samples. This study introduces an enzyme inhibition-mediated distance-based paper (EIDP) biosensor designed for naked-eye visual detection of OPs in food samples. We synthesized a copper alginate (Cu-Alg) hydrogel that traps water within the gel and restricts water flow on pH paper. When incubated with acetylcholinesterase (AChE) and acetylthiocholine (ATCh), the enzyme activity of AChE on ATCh generates thiocholine, which interacts with the Cu2+ ions in the gel. This interaction alters the gel’s 3D structure, releasing the trapped water onto the pH paper. Conversely, when AChE is exposed to OPs, its activity is inhibited, limiting the water flow from the gel. As a result, OPs are quantified by measuring the reduction in water flow distance within a linear range of 18 to 105 ng/mL, with a lower detection limit of 18 ng/mL. The EIDP biosensor exhibits high selectivity for OP detection and successfully analyzes OPs in pumpkin and rice samples, achieving percent recoveries ranging from 93% to 103%. This method offers a straightforward, portable, instrument-free, and cost-effective solution for detecting OPs in food samples.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 4","pages":"147-147"},"PeriodicalIF":0.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2227-9040/13/4/147/pdf?version=1744815257","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Polyphenol–Metal Film-Modified Colored Porous Microspheres for Enhanced Monkeypox Antigen Detection","authors":"Wei-Zhi Zhang, Chenfei Zhang, Shou‐Nian Ding","doi":"10.3390/chemosensors13040142","DOIUrl":"https://doi.org/10.3390/chemosensors13040142","url":null,"abstract":"The Monkeypox virus (MPXV), a DNA virus classified under the Orthpoxvirus genus alongside variola virus, has recently garnered significant global health attention due to its increasing transmission and emerging genomic mutations. Point-of-care testing is essential for effective clinical response and outbreak mitigation. In this article, we developed a novel class of colored microspheres designed for application in a lateral flow immunoassay (LFIA) platform targeting MPXV-specific biomarkers. Polystyrene-maleic anhydride (SMA-MAA) microspheres were synthesized with a high-temperature soap-free emulsion polymerization optimized in our lab. Subsequent alkali and acid treatments were employed to introduce porosity into the microsphere matrix. Solvent Red 27 and Disperse Red 60 were incorporated via solvent-swelling and thermal-swelling methods, respectively, to generate high brightness (HB) carriers. A surface coating composed of a tannic acid–iron (TA–Fe3⁺) coordination complex was applied to form a stable metal–polyphenol film (MPF). This coating not only minimized dye leaching by establishing a robust shell but also improved dye distribution, thereby enhancing overall color intensity. The final HB-LFIA system, configured in a sandwich immunoassay format, demonstrated favorable sensitivity and linear detection range for Monkeypox antigen, indicating strong potential for clinical diagnostic use.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 4","pages":"142-142"},"PeriodicalIF":0.0,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2227-9040/13/4/142/pdf?version=1744451532","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147890391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive Detection of Aflatoxin B1 in Foods Using Aptasensing Based on FGO-Mediated CdTe QDs","authors":"Puye Liang, Sihan Liu, Q. Han, Kaixuan Zhou, Tiange Li, Xianqing Huang, Lianjun Song, Tianlin Wang","doi":"10.3390/chemosensors13040141","DOIUrl":"https://doi.org/10.3390/chemosensors13040141","url":null,"abstract":"Aflatoxin B1 (AFB1) exhibits high toxicity and has the potential to induce cancer, deformities, and mutations. It is therefore highly desirable that sensitive and straightforward methods for detecting AFB1 be developed. In this study, due to the high specific adsorption capacity of AFB1 aptamers, we applied a sensing strategy based on quantum dots (QDs) and carboxyl-functionalized graphene oxide (FGO) to construct a simple fluorescence quenching platform. FGO and CdTe QDs modified with AFB1 aptamers cause a FRET effect that produces CdTe QDs with yellow-green fluorescence quenching. When AFB1 is present, aptamers form complexes with it and CdTe QDs leave the quenching platform, resulting in fluorescence recovery. In this study, we used a fluorescence aptasensor with a wide detection range of 0.05 to 150 ng/mL and a low limit of detection (LOD) of 8.2 pg/mL. The average recoveries of AFB1 in peanut and pure milk samples ranged from 94.5% to 107.0%. The aptasensor also exhibited the advantages of simple operation, low cost, and good stability. The sensing strategy reported here can thus serve as a potential candidate for the rapid detection of AFB1.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 4","pages":"141-141"},"PeriodicalIF":0.0,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2227-9040/13/4/141/pdf?version=1744364469","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147908952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Intensity of Bitter–Astringent Interactive Perception in Green Tea Based on the Weber–Fechner Law","authors":"Siying Li, Dongdong Du, Shaoming Cheng, Zhenbo Wei","doi":"10.3390/chemosensors13040137","DOIUrl":"https://doi.org/10.3390/chemosensors13040137","url":null,"abstract":"This study aimed to quantify the bitter–astringent interactive perceived intensity (BAIPI) of green tea based on the Weber–Fechner law by two-alternative forced choice test. Caffeine, (-)-epigallocatechin-3-gallate (EGCG), and Quercetin-3-o-rutinoside (Que-rut) were identified as the key bitter–astringent compounds. Then, BAIPI values were assessed based on the concentration ratios and ranges of caffeine, EGCG, and Que-rut in green tea according to the Weber–Fechner law. The BAIPI values were further fitted to a logarithmic curve: yBAIPIvalue=a×lnxconcentration of caffeine−b−⁡c+IVinitialBAIPIvalue−1. The constant terms a, b, and c were derived from the ratio of caffeine to EGCG, as the slope and X-intercept of the curves correlated exponentially with this ratio. The initial BAIPI value (IV) was calculated as: IVinitialBAIPIvalue=18.7960−0.3956q(the ratio of caffeine to Que−rut). Validation showed a standard error of 1.5865 between estimated and actual BAIPI values. This method enables the estimation of bitter–astringent intensity in green tea by analyzing the concentrations of caffeine, EGCG, and Que-rut.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 4","pages":"137-137"},"PeriodicalIF":0.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147331335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Ammonia Nitrogen in Neutral Aqueous Solutions Based on In Situ Modulation Using Ultramicro Interdigitated Array Electrode Chip","authors":"Yuqi Liu, Nan Qiu, Zhihao Zhang, Yang Li, Chao Bian","doi":"10.3390/chemosensors13040138","DOIUrl":"https://doi.org/10.3390/chemosensors13040138","url":null,"abstract":"In this study, an in situ electrochemical modulation method based on an ultramicro interdigitated array electrode (UIAE) sensor chip was developed for the detection of ammonia nitrogen (NH3-N) in neutral aqueous solutions. One comb of the UIAE was used as the working electrode for both the modulating and sensing functions, while the other comb was used as the counter electrode. Utilizing its enhanced mass transfer and proximity effects, the feasibility of in situ modulation of the solution environment near the UIAE chip to generate an electrochemical response for NH3-N was investigated using electrochemical methods. The proposed method enhances the concentration of hydroxide ions and active chloride in the local solution near the sensor chip. These reactive species play a key role in improving the sensor’s electrocatalytic oxidation capability toward ammonia nitrogen, facilitating the sensitive detection of ammonia nitrogen in neutral environments. A linear relationship was displayed, ranging from 0.15–2.0 mg/L (as nitrogen) with a sensitivity of 3.7936 µA·L·mg−1 (0.0664 µA µM−1 mm−2), which was 2.45 times that in strong alkaline conditions without modulation. Additionally, the relative standard deviation of the measurement remained below 2.9% over five days of repeated experiments, indicating excellent stability.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 4","pages":"138-138"},"PeriodicalIF":0.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2227-9040/13/4/138/pdf?version=1744268731","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147884723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of Au-CeO2 Nanocubes as a New SERS Substrate and Efficient Detection of Organic Compounds","authors":"Xinlong Tian, Li Ren, Jie Huang, Guangcheng Xi, Fei‐Fan Chang, Guoying Wei","doi":"10.3390/chemosensors13040135","DOIUrl":"https://doi.org/10.3390/chemosensors13040135","url":null,"abstract":"Surface-enhanced Raman scattering (SERS) is extensively employed for detecting organics, where its sensitivity and selectivity are strongly influenced by the properties of the SERS substrates. In this work, a simple hydrothermal synthesis followed by a subsequent reduction was used to prepare Au-CeO2 composite nanocubes as a new SERS substrate, in which the side length of the CeO2 cubes was 20~30 nm and the diameter of the Au nanoparticles was 5~25 nm. Using methylene blue (MB) and crystal violet (CV) as probe molecules, the lowest detection limit (LDL) of methylene blue (MB) on the Au-CeO2 composite nanocubes substrate was 10−7 M, and the maximum SERS enhancement factor (EF) was 2.6 × 105. As a result, the lowest detection limit (LDL) of crystal violet (CV) was 10−7 M, and the maximum enhancement factor (EF) was 3.7 × 104. The above results proved that the Au-CeO2 composite nanocubes had a quite good Raman enhancement effect, which could be used as a SERS substrate. Finally, a Raman enhancement mechanism is proposed for the Au-CeO2 nanucubes.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 4","pages":"135-135"},"PeriodicalIF":0.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing a Quantitative Profiling Method for Detecting Free Fatty Acids in Crude Lanolin Based on Analytical Quality by Design","authors":"Sihan Liu, Shaohua Wu, Hao Zhang, Xingchu Gong","doi":"10.3390/chemosensors13040126","DOIUrl":"https://doi.org/10.3390/chemosensors13040126","url":null,"abstract":"In this study, a quantitative profiling method for detecting free fatty acids in crude lanolin based on the Quality by Design (QbD) concept was developed. High-performance liquid chromatography (HPLC) equipped with a charged aerosol detector (CAD) and a Proshell 120 EC C18 column was employed for the separation of crude lanolin components. Initially, the analytical target profile and critical method attributes were defined. Potential critical method parameters, including column temperature, flow rate, isocratic run time, gradient end organic phase ratio, and gradient time, were identified using fishbone diagrams and single-factor experiments. The definitive screening design (DSD) was then utilized to screen and optimize these parameters. Stepwise regression was applied to establish quantitative models between the critical method attributes and the method parameters. Subsequently, the method operable design region (MODR) was calculated and was successfully verified. The analytical conditions established were configured with 0.1% formic acid in water and 0.1% formic acid in acetonitrile serving as the mobile phases. The flow rate was set at 0.8 mL/min, and the column temperature was maintained at 35 °C with the evaporation tube temperature also set at 35 °C. An injection volume of 10 μL was used for each analysis. The gradient elution conditions were as follows: from 0 to 30 min, 75% of solvent B was used, and from 30 to 60 min, the proportion of solvent B was increased from 75% to 79%. Ten components, including 12-hydroxystearic acid, 2-hexyldecanoic acid, and palmitic acid, were identified by mass spectrometry, and seven common peaks were found in the fingerprints. The contents of palmitic acid, oleic acid, and stearic acid in the crude lanolin were quantitatively determined. Both the fingerprint and quantitative analysis methods were validated. The method was applied to analyze 15 batches of crude lanolin from different sources. The new established quantitative profiling method for free fatty acids can be potentially used for industrial applications to enhance the quality control of crude lanolin.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 4","pages":"126-126"},"PeriodicalIF":0.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2227-9040/13/4/126/pdf?version=1743667751","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147895632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Photo-Controllable DNAzyme-Based Nanosensor for miRNA Imaging in Living Cells","authors":"Yanfei Zhang, Shujun Zhang, Rui-Ning Ouyang, Zong Dai, Si‐Yang Liu","doi":"10.3390/chemosensors13040123","DOIUrl":"https://doi.org/10.3390/chemosensors13040123","url":null,"abstract":"MircroRNA (miRNA) exhibits abnormal expression in many cancer diseases, and the detection and analysis of miRNA are significant for the early diagnosis of diseases and research on miRNA functions. In this work, we construct a UV-triggered DNAzyme (UTD) nanosensor for the early detection of miRNA in tumor cells. As the nanodevice was delivered into cells and irradiated by UV light, the controllable imaging of miRNA in living cells was achieved. This method effectively avoids false signal issues, providing a new strategy for high-spatiotemporal-resolution imaging of miRNA in living cells.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 4","pages":"123-123"},"PeriodicalIF":0.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147330422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-Way Fluorescence Technique Combined with Four-Way Calibration for the Determination of Thiabendazole and Carbaryl in Apple","authors":"Haiyan Chang, Hailong Wu, Tong Wang, Xiaozhi Wang, Ruqin Yu","doi":"10.3390/chemosensors13030107","DOIUrl":"https://doi.org/10.3390/chemosensors13030107","url":null,"abstract":"In this study, an excitation–emission–pH multi-way fluorescence technique coupled with a third-order calibration method based on an alternating quadrilinear decomposition (AQLD) algorithm was proposed for the simultaneous determination of thiabendazole (TBZ) and carbaryl (CAR) in apples. AQLD can be considered a “mathematical separation” technique that extracts the pure signal of the target analyte from complex mixed signals, thereby effectively addressing fluorescence peak overlap and unknown interference. The average spiked recoveries of the target analytes ranged from 98.4% to 101.9%, and the relative standard deviation was less than 5.6%. To evaluate the performance of the method, a number of parameters were calculated, including sensitivity (SEN), selectivity (SEL), limit of detection (LOD), limit of quantification (LOQ), and intra-day and inter-day precision. The results of the third-order calibration method were compared with those of the second-order calibration method (based on excitation–emission matrix fluorescence). These results showed that the former was superior. In short, the proposed strategy is simple, cost-effective, and anti-interference, providing a valuable reference for accurate quantification of TBZ and CAR in complex food matrices with uncalibrated interferences.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":"13 3","pages":"107-107"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2227-9040/13/3/107/pdf?version=1741967604","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147911703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}