一种高灵敏度、高选择性快速检测活细胞中外源性和内源性过氧亚硝酸盐的红色荧光探针

IF 3.7 3区工程技术 Q2 CHEMISTRY, ANALYTICAL

Chemosensors Pub Date : 2023-11-06 DOI:10.3390/chemosensors11110555

Bing Jin, Jing Liu, Longsheng Jin, Weishuai Liu, Xiangjun Liu

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引用次数: 0

摘要

过氧亚硝酸盐(ONOO−)在许多生理和病理过程中起着至关重要的作用，许多疾病已被证明与它的失调产生有关。荧光探针的发展满足了跟踪ONOO -的需要，并更好地了解其多种机制。在这项工作中，通过将rhodollike荧光团与酰肼反应位点功能化，合成了一种红色荧光探针BP-ONOO。在中性或弱碱性条件下，BP-ONOO对ONOO−具有高灵敏度、极好的选择性和短响应时间(小于4 s)。这些吸引人的特性有利于其在活细胞中ONOO -实时成像的应用，并且该探针已成功应用于药物刺激下RAW 264.7巨噬细胞中ONOO -浓度水平的成像。

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A Red-Emitting Fluorescence Probe for Rapid Detecting Exogenous and Endogenous Peroxynitrite in Living Cells with High Sensitivity and Selectivity

Peroxynitrite (ONOO−) has been revealed to play crucial roles in many physiological and pathological processes, and many diseases were proven to be associated with its misregulated production. The development of fluorescent probes meets the need for tracking ONOO− and gives a better understanding of its diverse mechanisms. In this work, a red-emitting fluorescent probe BP-ONOO was synthesized via functionalization of the rhodol-like fluorophore with a reactive site of hydrazide. The probe BP-ONOO exhibited high sensitivity, excellent selectivity, and short response time (less than 4 s) towards ONOO− under neutral or weak alkaline conditions. These attractive properties favor its application in real-time imaging of ONOO− in living cells, and the probe has been successfully applied for imaging the concentration levels of ONOO− in RAW 264.7 macrophage cells under drug stimulation.

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来源期刊

Chemosensors Chemistry-Analytical Chemistry

CiteScore

5.00

自引率

9.50%

发文量

450

审稿时长

11 weeks

期刊介绍： Chemosensors (ISSN 2227-9040; CODEN: CHEMO9) is an international, scientific, open access journal on the science and technology of chemical sensors published quarterly online by MDPI.The journal is indexed in Scopus, SCIE (Web of Science), CAPlus / SciFinder, Inspec, Engineering Village and other databases.