Genomic Medicine, Biomarkers, and Health Sciences最新文献_第2页

Effectiveness of great burdock essence compounds in the adjuvant treatment of gastric ulcer patients infected with Helicobacter pylori 大牛蒡精华化合物辅助治疗幽门螺杆菌感染胃溃疡的疗效观察

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2012-09-01 DOI: 10.1016/j.gmbhs.2012.09.001

Hsueh-Chiao Liu , Ming-Kun Ku , Fu-Yen Chung , Ching-Chiang Lin , Shiu-Ru Lin

{"title":"Effectiveness of great burdock essence compounds in the adjuvant treatment of gastric ulcer patients infected with Helicobacter pylori","authors":"Hsueh-Chiao Liu , Ming-Kun Ku , Fu-Yen Chung , Ching-Chiang Lin , Shiu-Ru Lin","doi":"10.1016/j.gmbhs.2012.09.001","DOIUrl":"10.1016/j.gmbhs.2012.09.001","url":null,"abstract":"<div>Helicobacter pylori commonly infects the human stomach lining is one of the risk factors that can lead a gastric ulcer to develop into gastric cancer. Studies have found that burdock can enhance the protection of gastrointestinal mucosa with antibacterial property that can reduce the damage of the stomach by H pylori. Great burdock essence compounds are prepared using a nanomicell formulation, which contains Arctium lappa Linn, Arnebia euchroma, and Angelica sinensis. This study aims to assess the effectiveness of great burdock essence compounds in the adjuvant treatment of H pylori infected clinical patients. Forty-four patients with gastric ulcers, 21 of whom were taking great burdock essence compounds and 23 taking placebo, were included in this study. After taking great burdock essence compounds or placebo for 1 month, 13C-Urea Breath Test showed 20 (20/21) patients with negative results and only one person with positive results among the burdock group; in addition, it also indicated that there were 1 6(16/23) with negative results and seven (7/23) patients with positive results among the placebo group. The statistical results also showed that the positive 13C-Urea Breath Test results in patients who took great burdock essence compounds were significantly lower than that of the control group (p = 0.027). It suggested that great burdock essence is an appropriate health supplement for gastric ulcer patients with H pylori infection.</div>","PeriodicalId":100577,"journal":{"name":"Genomic Medicine, Biomarkers, and Health Sciences","volume":"4 3","pages":"Pages 81-84"},"PeriodicalIF":0.0,"publicationDate":"2012-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gmbhs.2012.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78661165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Development of mini-Sep for nucleated cell purification and isolation 有核细胞纯化分离用mini-Sep的研制

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2012-03-01 DOI: 10.1016/j.gmbhs.2012.04.020

Wan-Ju Kung , Ching-Chiang Lin , Shiu-Ru Lin

引用次数: 0

Significance of migration-related genes (S100A9, MAGED4, C8orf30A, IL-8) in esophageal squamous cell carcinoma 迁移相关基因S100A9、MAGED4、C8orf30A、IL-8在食管鳞状细胞癌中的意义

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2012-03-01 DOI: 10.1016/j.gmbhs.2012.04.002

Ching-Tang Huang , Guan-Cheng Huang , Pey-Shan Wu , Li-Wha Wu , Siao-Han Lin , Wu-Wei Lai , Yi-Ching Wang , Hsiao-Sheng Liu

{"title":"Significance of migration-related genes (S100A9, MAGED4, C8orf30A, IL-8) in esophageal squamous cell carcinoma","authors":"Ching-Tang Huang , Guan-Cheng Huang , Pey-Shan Wu , Li-Wha Wu , Siao-Han Lin , Wu-Wei Lai , Yi-Ching Wang , Hsiao-Sheng Liu","doi":"10.1016/j.gmbhs.2012.04.002","DOIUrl":"10.1016/j.gmbhs.2012.04.002","url":null,"abstract":"<div>To identify any biomarkers related to the migration of esophageal squamous cell carcinoma (ESCC) cells, ESCC CE81T cells were used to establish the CE81T-1 subline, which demonstrates increased migration activity after Transwell screening and microarray analysis. Among the differentially expressed genes, S100A9 was most downregulated, and MAGED4, C8orf30A, and IL-8 were the most upregulated in CE81T-1 cells. The expression of these four genes at the mRNA level was validated using the ESCC CE81T and KYSE cell lines and clarified in ESCC specimens using real-time polymerase chain reaction. Among 60 pairs of ESCC specimens (normal and tumor specimens), the expression level of S100A9 mRNA was significantly lower in the tumor sections in comparison with the normal sections (p  =0.0061). Furthermore, C8orf30A expression was significantly correlated with ESCC metastatic status (p  =0.036), as determined by Kaplan-Meier analysis. Functional studies revealed that S100A9 plays a suppressive role in the proliferation and migration of ESCC cells through the overexpression of ectopic S100A9 and small interfering RNA, as determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assays. Altogether, this study reveals that C8orf30A has the potential to be used as a novel biomarker for the prognosis for ESCC metastasis and survival. Furthermore, the IL-8 and S100A9 genes may have potential in ESCC diagnosis.</div>","PeriodicalId":100577,"journal":{"name":"Genomic Medicine, Biomarkers, and Health Sciences","volume":"4 1","pages":"Pages 16-18"},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gmbhs.2012.04.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73435411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The use of multiple molecular markers as predictors of the clinical prognosis of patients with colorectal cancer 多分子标志物作为结直肠癌患者临床预后预测指标的应用

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2012-03-01 DOI: 10.1016/j.gmbhs.2012.04.021

Ting-Chen Tung , Shiu-Ru Lin , Jaw-Yuan Wang , Fu-Yen Chung

{"title":"The use of multiple molecular markers as predictors of the clinical prognosis of patients with colorectal cancer","authors":"Ting-Chen Tung , Shiu-Ru Lin , Jaw-Yuan Wang , Fu-Yen Chung","doi":"10.1016/j.gmbhs.2012.04.021","DOIUrl":"10.1016/j.gmbhs.2012.04.021","url":null,"abstract":"<div>Serum carcinoembryonic antigen (CEA) is most commonly used as a prognostic biomarker for evaluating curatively resected colorectal cancer (CRC) patients, but it has a low sensitivity and specificity. The aim of this study was to evaluate potential genetic markers in CRC patients using membrane array. Fifty CRC patients were enrolled and mRNA expression in their tissues were analyzed using membrane array analysis. Seven genes were analyzed in this study, including ATP2A2, GLUT1, MMP13, MAGE-A2, MAGE-A7, MAGE-A8, and MAGE-A12. Correlations between the results of the membrane array and the clinicopathological features of these CRC patients were then evaluated. The results show that the overexpression of any three or four of these seven genes is correlated with tumor invasion depth, lymphatic invasion, advanced stage, and postoperative recurrence (all p < 0.005). Furthermore, the expression of any four genes was more significantly correlated with clinicopathological characteristics than the expression of only two or three genes. The combination of multiple molecular markers and the membrane array method might be useful for predicting postoperative relapse in CRC patients.</div>","PeriodicalId":100577,"journal":{"name":"Genomic Medicine, Biomarkers, and Health Sciences","volume":"4 1","pages":"Pages 30-33"},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gmbhs.2012.04.021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72747517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Multiple mRNA markers for the detection of circulating tumor cells in breast cancer patients 多种mRNA标记物检测乳腺癌患者循环肿瘤细胞

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2012-03-01 DOI: 10.1016/j.gmbhs.2012.04.012

Hsu-Chin Hung , Li-Chen Yen , Shiu-Ru Lin , Jaw-Yuan Wang

{"title":"Multiple mRNA markers for the detection of circulating tumor cells in breast cancer patients","authors":"Hsu-Chin Hung , Li-Chen Yen , Shiu-Ru Lin , Jaw-Yuan Wang","doi":"10.1016/j.gmbhs.2012.04.012","DOIUrl":"10.1016/j.gmbhs.2012.04.012","url":null,"abstract":"<div>The detection of circulating tumor cells (CTCs) is believed to be a potential method for the early detection of cancer. The aim of this study was to employ a membrane array to develop a panel of mRNA markers for the detection of CTCs in breast cancer patients. Twenty genes with highly differentiated expression levels were selected from microarray studies. The subsequent validation analysis of 30 pairs of breast tissue samples showed that 19 genes demonstrated two-fold overexpression in cancer tissues in comparison with those in normal tissues in 80% of the paired samples. Furthermore, a membrane array experiment was conducted on blood samples from 64 normal controls and 87 breast cancer patients, and the predictive power of each gene was evaluated by analyzing the gene expression level using receiver-operating curves (ROC). Among the 20 genes selected, eight genes that demonstrated the largest areas under the curve (AUC) > 0.8 were selected as diagnostic markers. According to the ROC analysis, when setting the cut-off point of five positive genes, the genetic array of the eight markers was recognized as positive with sensitivity and specificity values of 90% and 89%, respectively. These results suggest that this mRNA marker array could be useful for detecting CTCs in breast cancer patients.</div>","PeriodicalId":100577,"journal":{"name":"Genomic Medicine, Biomarkers, and Health Sciences","volume":"4 1","pages":"Pages 34-37"},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gmbhs.2012.04.012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90510720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Proteomic analysis of quercetin-induced cardioprotective effects 槲皮素诱导的心脏保护作用的蛋白质组学分析

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2012-03-01 DOI: 10.1016/j.gmbhs.2012.04.006

Jing-Yi Chen , Hong-Lin Chan , Hsiu-Chuan Chou

引用次数: 3

Development of great burdock essence compounds 牛蒡精华化合物的开发

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2012-03-01 DOI: 10.1016/j.gmbhs.2012.04.016

Ching-Hui Wang , Fu-Yen Chung , Tong-Rong Tsai , Shiu-Ru Lin , Hsueh-Chiao Liu

引用次数: 2

Combining MALDI-TOF and molecular imaging with principal component analysis for biomarker discovery and clinical diagnosis of cancer 将MALDI-TOF与分子成像结合主成分分析用于肿瘤生物标志物的发现和临床诊断

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2012-03-01 DOI: 10.1016/j.gmbhs.2012.04.022

Yi-Tzu Cho , Yi-Yan Chiang , Jentaie Shiea , Ming-Feng Hou

{"title":"Combining MALDI-TOF and molecular imaging with principal component analysis for biomarker discovery and clinical diagnosis of cancer","authors":"Yi-Tzu Cho , Yi-Yan Chiang , Jentaie Shiea , Ming-Feng Hou","doi":"10.1016/j.gmbhs.2012.04.022","DOIUrl":"10.1016/j.gmbhs.2012.04.022","url":null,"abstract":"<div>Molecular imaging using matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF MS) is effective for determining the distribution of molecules of interest in specific tissues. It can determine the direct correlation between metabolite, lipid, and protein expression and histology. Principle component analysis (PCA) can reduce the dimensions of a data set while still retaining the information present in the original data set. Using PCA to process MALDI data, samples with different statuses and ion patterns on their MALDI mass spectra can be classified, grouped, and evaluated on the same score plot. The use of MALDI-TOF in combination with PCA to compare the lipid, peptide, and protein profiles of different biological specimens can then be used to diagnose disease. Because ions with significant differences between sampling regions in a tissue can be indicated using PCA, the imaging of these “interesting peaks” can be visualized by plotting the ion intensity across the tissue section.</div>","PeriodicalId":100577,"journal":{"name":"Genomic Medicine, Biomarkers, and Health Sciences","volume":"4 1","pages":"Pages 3-6"},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gmbhs.2012.04.022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80580847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Ruptured ovarian yolk sac tumor combined with hemoperitoneum in a young girl with abdominal blunt injury 卵巢卵黄囊破裂并腹膜出血一例年轻女孩腹部钝性损伤

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2012-03-01 DOI: 10.1016/j.gmbhs.2012.04.011

Chin-Fan Chen , Wee-Yeen Wong , Chieh-Han Chuang , Yung-Sung Yeh , Kun-Bow Tsai , Jaw-Yuan Wang

{"title":"Ruptured ovarian yolk sac tumor combined with hemoperitoneum in a young girl with abdominal blunt injury","authors":"Chin-Fan Chen , Wee-Yeen Wong , Chieh-Han Chuang , Yung-Sung Yeh , Kun-Bow Tsai , Jaw-Yuan Wang","doi":"10.1016/j.gmbhs.2012.04.011","DOIUrl":"10.1016/j.gmbhs.2012.04.011","url":null,"abstract":"<div>We report our clinical experience concerning the incidental finding of a ruptured ovarian yolk sac tumor combined with hemoperitoneum in a patient with abdominal blunt injury. An girl 8 years of age visited emergency department due to vomiting and persistent abdominal pain after abdominal blunt injury. Physical examination revealed pale conjunctiva, abdominal distension, and tenderness over bilateral lower abdomen without any obvious peritoneal sign. After stabilization of her condition, one huge pelvic tumor with ascites was identified by abdominal sonography and computed tomography scan. Subsequently, hemoperitoneum was confirmed by paracentesis procedure. During exploratory laparotomy, one ruptured left ovarian tumor with bleeding was identified. Therefore, she underwent left oophorectomy and left salpingectomy. Typical structures of Schiller-Duval bodies and hyaline globules in the tumor cells were identified. Biopsy of the omental tissues also confirmed metastatic lesions of the tumor. Ovarian yolk sac tumor (FIGO Stage 3) was confirmed. She received four courses of chemotherapy after the operation and received subsequent regular follow-up at the hospital with an uneventful recovery. This case highlights the possibility of ruptured ovarian yolk sac tumor after abdominal blunt injury as the cause of internal bleeding, and it should be taken into consideration as one possible etiology of hemoperitoneum in a young girl.</div>","PeriodicalId":100577,"journal":{"name":"Genomic Medicine, Biomarkers, and Health Sciences","volume":"4 1","pages":"Pages 76-78"},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gmbhs.2012.04.011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82243784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Pseudozyma spp. and Barnettozyma spp. effectively kill cancer cells in vitro 假酵素和巴奈特菌酵素在体外能有效杀死癌细胞

Genomic Medicine, Biomarkers, and Health Sciences Pub Date : 2012-03-01 DOI: 10.1016/j.gmbhs.2012.04.015

Ren-Yu Hu, Ching-Fu Lee, Hsiu-Chuan Chou

{"title":"Pseudozyma spp. and Barnettozyma spp. effectively kill cancer cells in vitro","authors":"Ren-Yu Hu, Ching-Fu Lee, Hsiu-Chuan Chou","doi":"10.1016/j.gmbhs.2012.04.015","DOIUrl":"10.1016/j.gmbhs.2012.04.015","url":null,"abstract":"<div>Cancer is the overall leading cause of death in developed countries and also worldwide, and being able to exploit an effective anticancer drug is the aim of all cancer scientists. However, many of the synthetic drugs produced so far usually cause serious side effects, which reduces their therapeutic efficacy. Discovering new drugs or auxiliary therapies derived from natural products might thus provide a novel opportunity for cancer therapy. A recent study reported that some lethal toxins can maintain their activity after being injected into mice. We therefore used two Pseudozyma spp. and three Barnettozyma spp. to examine whether these killer yeasts can preserve their lethal effect on cancer cells under the physical environment (optimum pH, temperature and osmolality, supporting a living cell accomplishes to proliferate, metabolize, differentiate and survive). Our preliminary results showed that both Barnettozyma spp. and Pseudozyma spp. have stronger cytotoxicity against HepG2 than Chang’s liver cells. According to the results of two-dimensional difference gel electrophoresis (2D-DIGE), a total of 115 and 27 proteins differentially expressed by 1.5-fold or more were observed for HepG2 and Chang’s liver cells, respectively. Furthermore, we explored the mechanism involved in the effect of the lethal yeast filtrates on liver cancer cells using 2D-DIGE and mass spectrometry.</div>","PeriodicalId":100577,"journal":{"name":"Genomic Medicine, Biomarkers, and Health Sciences","volume":"4 1","pages":"Pages 61-64"},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gmbhs.2012.04.015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73943302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1