Development of mini-Sep for nucleated cell purification and isolation

Abstract

Despite the availability of various methods that can isolate nucleated cells from blood or body fluids, these methods still require centrifugation and other purification devices to work. The main purpose of this study was to develop a simple cell purification and isolation device (mini-Sep) that does not require any centrifugation equipment to operate and can be used to isolate and concentrate nucleated cells from blood or body fluids for use in cell-related experiments. Mini-Sep works by using a syringe or pump to inject samples, thereby filtering out any impurities by removing residual nucleated cells and holding them in a containment space that was designed as part of the device. The results show that mini-Sep possesses the capability to isolate and concentrate nuclear cells, can be operated in simple and easy steps, and saves time typically spent on centrifugation. It can be easily adapted for use in various laboratories and places that lack laboratory equipment.