Dentistry Review最新文献

{"title":"Oral Microbially Induced Exosomes promote Neuroinflammation and Microglial Cell Senescence","authors":"Cayla Paradise,&nbsp;Ranya Elsayed PhD MBA","doi":"10.1016/j.dentre.2024.100110","DOIUrl":"10.1016/j.dentre.2024.100110","url":null,"abstract":"<div><h3>OBJECTIVES</h3><p>Porphyromonas gingivalis (Pg) and its gingipain proteases contribute to Alzheimer's disease (AD) pathogenesis through yet unclear mechanisms. Cellular secretion of small extracellular vesicles or exosomes (exo) increases with aging as part of the senescence-associated secretory phenotype (SASP). We have shown that exo isolated from Pg-infected dendritic cells (PgDCexo) contain Pg antigens, transmit senescence to bystander gingival cells, and can cross the blood-brain barrier in mice. This study aims to determine the ability of PgDCexo to induce senescence and neuroinflammation in the brain using a microglial cell line in vitro.</p></div><div><h3>METHODS</h3><p>Isolated PgDCexo were quantitated and characterized using NTA, western blot (WB), and transmission electron microscopy (TEM). DiI labeled PgDCExo were co-cultured with murine SIMA9 microglial cells for 24 hrs, and uptake was analyzed by confocal microscopy. The effect of PgDCexo on microglial senescence and inflammation was tested using western blot, qPCR, and flow cytometry analysis (FACS).</p></div><div><h3>RESULTS</h3><p>DiI labeled PgDCexo were internalized in microglial cells. PgDCexo induced senescence in SIMA9 cells in a dose-dependent manner as shown by an increase in senescence biomarkers, p16 INK4A and P53 by WB and qPCR . SIMA9 cells treated with PgDCExo showed upregulated levels of IL-1β, TNFa and IL-6 by FACS and activated NLRP3 inflammasome pathway by WB.</p></div><div><h3>CONCLUSIONS</h3><p>Extracellular vesicles induced by P. gingivalis infection promote senescence and inflammation in microglial cells. These results suggest a potential role of Pg-induced extracellular vesicles in neuroinflammation and AD pathogenesis.</p></div><div><h3>IMPLICATIONS</h3><p>This study sheds light on a novel mechanism through which P.gingivalis-induced extracellular vesicles affect neurodegenerative processes in Alzheimer's disease.</p></div>","PeriodicalId":100364,"journal":{"name":"Dentistry Review","volume":"4 3","pages":"Article 100110"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772559624000336/pdfft?md5=5803fe22d71a93e6d8faabedfa75c3b1&pid=1-s2.0-S2772559624000336-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142168736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Suture Technique to Stabilize Vestibule for a Double Laterally Positioned Flap","authors":"Kevin A. Shepherd,&nbsp;Mira Ghaly DMD MS","doi":"10.1016/j.dentre.2024.100138","DOIUrl":"10.1016/j.dentre.2024.100138","url":null,"abstract":"<div><h3>OBJECTIVES</h3><p>The goal of treatment is to establish a band of attached keratinized tissue to facilitate pain free oral hygiene and to eliminate the muscle attachment in order to help mitigate future recession.</p></div><div><h3>METHODS</h3><p>A 62yo white female presented with RT2 recession on teeth numbers 24,25. Due to extensive use of antiplatelet medication, the decision to limit surgical sites to one was made, ruling out free tissue grafts. The surgical plan consisted of preparing two partial thickness flaps and securing them over the deficient sites of 24,25. To maintain good adaptation of the flap to the periosteum, a 4.0 PTFE suture was used to secure the flaps by using the adjacent periosteum and teeth as anchors points. To obtain hemostasis, microfibrillar collagen was used directly on the site. The patient was followed up at one week and one month for post operative evaluation.</p></div><div><h3>RESULTS</h3><p>The evaluation at one month revealed elimination of the aberrant frenum and the re-establishment of a 4mm healthy band of attached keratinized tissue. During the healing there was no evidence of any flap necrosis as the flap was adequately secured against the periosteum.</p></div><div><h3>CONCLUSIONS</h3><p>Using the Periosteal-Tooth Anchor suture technique, the laterally positioned flaps were able to be secured in a manner that ensured survivability of the flaps and established a band of attached keratinized gingiva.</p></div><div><h3>IMPLICATIONS</h3><p>By informing clinicians of this suture technique, it will improve the predictability of these demanding and technique sensitive cases.</p></div>","PeriodicalId":100364,"journal":{"name":"Dentistry Review","volume":"4 3","pages":"Article 100138"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772559624000610/pdfft?md5=93815d5715d97c0ad620a1a634da938e&pid=1-s2.0-S2772559624000610-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do Silver Nanoparticles in Fixed Orthodontic Appliances Inhibit Bacterial Growth?","authors":"Kailyn Owens,&nbsp;Anasha Williamson,&nbsp;Ashley Christman RDH, BS","doi":"10.1016/j.dentre.2024.100120","DOIUrl":"10.1016/j.dentre.2024.100120","url":null,"abstract":"<div><h3>OBJECTIVES</h3><p>The purpose of this literature review is to aid in oral hygiene instruction for clinicians with patients undergoing orthodontic treatment.</p></div><div><h3>METHODS</h3><p>Specific criteria were required to formulate this literature review. Articles were accessed through PubMed and were peer-reviewed, original research that were published within the last five years, and relevant to the topic. Keywords for this research included orthodontics, fixed orthodontic appliances, orthodontic appliances, silver nanoparticles, silver coating, nanoparticles, bioreduction, oral bioreduction, and plaque reduction. A section from one published book was also referenced for background and comparative information. Filters for our search excluded articles posted before 2018, literature reviews, meta-analyses, or articles that were not peer reviewed. Four articles were selected to be included in this review.</p></div><div><h3>RESULTS</h3><p>Fabricated wires, brackets, and orthodontic bands were organized into control and experimental groups. In some groups, the metal was coated with silver nanoparticles, some had zinc oxide nanoparticles, and some were left uncoated. The appliances were exposed to oral bacteria for various amount of time and then tested to see which metals accumulated the most bacteria. In one study, a combination of silver and zinc-oxide nanoparticles produced the greatest bacterial inhibition. However, each study proved silver nanoparticle coated appliances resulted in the least amount of bacterial accumulation with all bacteria tested.</p></div><div><h3>CONCLUSIONS</h3><p>The reduction of plaque, bacteria, gingivitis, and bracket scars during and after orthodontic treatment is a direct result of silver nanoparticles and increases patient compliance to commit to an oral hygiene routine.</p></div><div><h3>IMPLICATIONS</h3><p>Silver nanoparticles can be implemented throughout all aspects of dentistry in toothpastes, mouthwashes, and other dentifrices. Hygienists, dental professionals, and patients can benefit from the antimicrobial effects of silver nanoparticles.</p></div>","PeriodicalId":100364,"journal":{"name":"Dentistry Review","volume":"4 3","pages":"Article 100120"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772559624000439/pdfft?md5=c6b7415d334b63a4bbfcedc08db14471&pid=1-s2.0-S2772559624000439-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects Of Enamel Matrix Derivative On Periodontal Patients","authors":"Abbi Rushton,&nbsp;Maddie White,&nbsp;Krystal Herring RDH, MPH","doi":"10.1016/j.dentre.2024.100102","DOIUrl":"10.1016/j.dentre.2024.100102","url":null,"abstract":"<div><h3>OBJECTIVES</h3><p>The purpose of this literature review was to describe enamel matrix derivative (EMD) and how it benefits periodontal patients. EMD's rejuvenating abilities were identified as well as the different functions of EMD.</p></div><div><h3>METHODS</h3><p>Articles chosen for this literature review were randomized control trials that were peer reviewed and published within the last five years. PubMed was accessed through Augusta University's database for research. Keywords used to search included enamel matrix derivative, bone loss, periodontitis, enamel matrix proteins, gum disease, regeneration, therapy, and growth.</p></div><div><h3>RESULTS</h3><p>Studies chosen for this literature review all showed that EMD improves clinical attachment level. It is able to promote hard and soft tissue regeneration because it contains the same proteins that are involved in amelogenesis. These proteins stimulate fibroblasts, anti-inflammatory cytokines, and clotting factors. All these factors make EMD a great adjunct to periodontal surgical procedures. EMD is also able to reduce inflammation, promote wound healing, and stabilize blood clots.</p></div><div><h3>CONCLUSIONS</h3><p>Overall, EMD's ability to assist in the formation of new bone, cementum, and periodontal ligaments makes it an excellent treatment option for periodontally involved patients. It is frequently used with scaling and root planing, flap surgery, and the treatment of infrabony defects.</p></div>","PeriodicalId":100364,"journal":{"name":"Dentistry Review","volume":"4 3","pages":"Article 100102"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772559624000257/pdfft?md5=21c602bb1af224ea6d00b2b2e5169413&pid=1-s2.0-S2772559624000257-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis identifies CCDC86 as a new potential Head and Neck Squamous Cell Carcinoma biomarker","authors":"Daniel Lee,&nbsp;Jules Gouron,&nbsp;Jules Gouron,&nbsp;Henrique Shimaoka Chagas,&nbsp;Bidhan Bhandari,&nbsp;Sahar Emami Naeini,&nbsp;Babak Baban,&nbsp;Pablo Shimaoka Chagas,&nbsp;Evila Salles","doi":"10.1016/j.dentre.2024.100105","DOIUrl":"10.1016/j.dentre.2024.100105","url":null,"abstract":"<div><h3>OBJECTIVES</h3><p>Overall, this comprehensive analysis aims to evaluate the expression profile of CCDC86 in Head and Neck Squamous Cell Carcinoma, providing insights into its potential as a diagnostic biomarker.</p></div><div><h3>METHODS</h3><p>Initially, we conducted transcriptomic integration analysis to examine CCDC86 expression in HNSCC using various datasets (RNA-seq) obtained from the gene expression profiling interactive analysis database. We also established the diagnostic significance of CCDC86 and coregulated genes in HNSCC through the Kaplan–Meier Plotter.</p></div><div><h3>RESULTS</h3><p>Initially, we detected elevated levels of CCDC86 in HNSCC, which correlated with lower survival rates (p=&lt;0.001). We also identified seven genes coregulated with CCDC86 that were also overexpressed in HNSCC in comparison with normal tissues.</p></div><div><h3>CONCLUSIONS</h3><p>For the first time, our findings suggest that CCDC86 can be a new emerging biomarker with promising therapeutic values to HNSCC patients.</p></div><div><h3>IMPLICATIONS</h3><p>No further implications other than specified in conclusion.</p></div>","PeriodicalId":100364,"journal":{"name":"Dentistry Review","volume":"4 3","pages":"Article 100105"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772559624000282/pdfft?md5=38c1a1f72a7925c967bf13070598fd5e&pid=1-s2.0-S2772559624000282-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adenosine A2a receptor in Experimental Periodontitis","authors":"Nathalie Paladines,&nbsp;Ana Carolina Morandini DDS MDS PhD","doi":"10.1016/j.dentre.2024.100106","DOIUrl":"10.1016/j.dentre.2024.100106","url":null,"abstract":"<div><h3>OBJECTIVES</h3><p>Our previous work has shown the role of adenosine in mitochondrial health to regulate IL-1-mediated inflammation in human gingival fibroblasts, and the protective role of enzyme CD73, which is the final step to generate adenosine extracellularly, in mediating osteoclastogenesis and the hyper inflammatory response of gingival fibroblasts. Adenosine A2a receptor (A2aR) agonist (CGS21680) has been associated with anti-inflammatory effects in models of sepsis, articular chondrocyte inflammation, and oral mucositis in vitro as well as particle-induced inflammatory bone destruction in vivo. This study examined the effects of A2aR agonist CGS 21680 in a mouse model of ligature-induced periodontitis.</p></div><div><h3>METHODS</h3><p>Mature adult mice (C57Bl/6J) underwent ligature placement on the maxillary second molar to induce periodontitis. The unligated contralateral molar tooth served as an internal control. For the following 8 days, the mice received intra-peritoneal injections of A2aR agonist (CGS21680, 0.1 mg/Kg) or a saline control. After 8 days, gingival tissues and maxillae were harvested. The maxillae underwent micro-CT analysis to measure alveolar bone loss and the gingival tissues were processed for protein analysis through immunoblot.</p></div><div><h3>RESULTS</h3><p>Micro-CT analysis demonstrated mice that received A2aR agonist had significantly less bone loss compared to the control group. Protein analysis of gingival tissue showed periodontitis induced higher IL-1 levels compared to the unligated side and decreased IL-1b in A2aR-treated mice compared to control animals.</p></div><div><h3>CONCLUSIONS</h3><p>In conclusion, A2aR-treated mice were protected from ligature-induced periodontitis and displayed less gingival IL-1 levels.</p></div>","PeriodicalId":100364,"journal":{"name":"Dentistry Review","volume":"4 3","pages":"Article 100106"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772559624000294/pdfft?md5=783b39d6bf4d59da002b5862bd709b25&pid=1-s2.0-S2772559624000294-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Will CAD/CAM Technology Increase the Effectiveness of Clinicians?","authors":"Shantell Boyd,&nbsp;Sonyetta Lorenzo-Jenkins,&nbsp;Trina Hutto RDH, MS, EdD","doi":"10.1016/j.dentre.2024.100116","DOIUrl":"10.1016/j.dentre.2024.100116","url":null,"abstract":"<div><h3>OBJECTIVES</h3><p>CAD/CAM stands for computer-aided design/computer-aided manufacturing. It is a digital system that employs CAM to translate designs from CAD into tangible goods. CAD/CAM intraoral scanners aim to make practitioners more effective in dentistry.</p></div><div><h3>METHODS</h3><p>The criteria selection is based on a study conducted with research articles. These articles were included and/or excluded based on the following criteria: articles should be peer reviewed, not published before 2018 and not literature reviews or meta-analysis. The database used to conduct this literature review was PubMed through Augusta University's online library database. The keywords used to find relevant articles were CAD/CAM, computer-aided design, computer-aided manufacturing, computer-assisted manufacturing, accuracy, intraoral scanner, high resolution, anxiety, stress, fear, nervousness, dental phobia, time distribution, time administration, effective planning, and time management.</p></div><div><h3>RESULTS</h3><p>To demonstrate that CAD/CAM improves clinician accuracy and efficacy, three separate studies were carried out. In the first study, an intraoral scanner is used to examine the accuracy of digital impressions at the edge of crown preparation using different scanning methodologies. A mandibular first molar was created entirely of ceramic on a typodont. Three settings—high solution, ordinary resolution, and combination resolution—were used to get the desired impression. Study two was carried out. Five skilled doctors and five less skilled clinicians were randomly allocated to build interim single crowns using digital sextant imaging. Research 3 found that using CAD/CAM would improve implant placement when comparing free-hand surgery to using the s-CAIS surgical guide for insertion.</p></div><div><h3>CONCLUSIONS</h3><p>Any intraoral scanner's accuracy is entirely dependent on the machine's hardware and is tuned for default scans. Compared to a traditional workflow, a single crown made with CAD/CAM needed less fabrication time and produced a better fit. competent to materialize dental implants, removable prosthodontics, veneers, crowns, inlays, onlays, and full mouth rehabilitation.</p></div><div><h3>IMPLICATIONS</h3><p>This stage of the scanning process is undesired because it requires more time to digitally trim away the preparation margin and set up a High-resolution mode in the program. Additionally, the increasing volume of computer data required to create scans makes this step necessary. Research II In a traditional workflow, less experienced Clinicians took longer to make temporary crowns. Study 3: To avoid errors resulting from provider omission, clinicians need to be trained in the proper use of CAD/CAM.</p></div>","PeriodicalId":100364,"journal":{"name":"Dentistry Review","volume":"4 3","pages":"Article 100116"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772559624000397/pdfft?md5=a191bbe4010f9853c50246d4491764a0&pid=1-s2.0-S2772559624000397-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142168723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benign Myoepithelial Neoplasms of Salivary Glands: Clinicopathologic Profile and Literature Review","authors":"Christopher Hollingsworth,&nbsp;Rafik A. Abdelsayed DDS, BDS, MS,&nbsp;Mohammed Bindakhil DDS, MS","doi":"10.1016/j.dentre.2024.100109","DOIUrl":"10.1016/j.dentre.2024.100109","url":null,"abstract":"<div><h3>OBJECTIVES</h3><p>This article aims to expand the literature on myoepitheliomas by describing several cases with an emphasis on histological presentation, clinical appearance, and demographics. To the best of our knowledge, this article is the largest single-center case series of myoepitheliomas.</p></div><div><h3>METHODS</h3><p>A retrospective search for myoepithelioma salivary gland tumors in the oral and maxillofacial pathology archive at The Dental College of Georgia was performed. Patient demographics and the clinicopathologic features were collected and analyzed. Additionally, a comprehensive literature review of benign myoepithelioma of the salivary glands was conducted. The database PubMed was searched from (1/1/2008-to-9/1/2023) using the search terms: “salivary gland”, “myoepithelioma.” Demographic and clinicopathologic data were extracted and analyzed. Descriptive analysis was performed using SPSS Statistics Version 26 (IBM Corporation, NY). Categorical data was analyzed through the Fisher exact test. Results were considered statistically significant at (P &lt; .05).</p></div><div><h3>RESULTS</h3><p>Institutional Case Series: The retrospective search revealed 13 cases of myoepithelial salivary gland tumors—9 benign myoepitheliomas &amp; 4 malignant myoepithelial tumors (included for comparison). Tumor morphology was analyzed—epithelioid pattern, 6 cases (4 benign, 2 malignant); spindle cell pattern, 6 cases (4 benign, 2 malignant); plasmacytoid pattern, 7 cases (6 benign, 1 malignant). The characteristics of connective tissue trauma were also analyzed. The stroma was myxoid in 2 cases (1 benign, 1 malignant), fibrinous in 5 cases (4 benign, 1 malignant), and necrotic in 2 cases (2 malignant). The difference in reported clinicopathologic characteristics was not statistically significant, except for the presence of mitosis (p=.001).</p></div><div><h3>CONCLUSIONS</h3><p>Rare salivary gland tumors, especially those arising in atypical locations with ambiguous morphological patterns, present a unique diagnostic challenge. Due to myoepithelioma's ability to manifest in the various glandular structures of the head and neck, dental professionals and physicians who encounter head and neck pathology must recognize this entity as part of their differential diagnosis when clinically appropriate.</p></div><div><h3>IMPLICATIONS</h3><p>This project is targeted at clinicians and pathologists who encounter and manage head and neck pathology. Due to the rarity of this tumor class, there are many case reports, but no case series or reviews of this magnitude providing a clear profile of this neoplasm. The value of this article lies in the scope of our data set and the clinicopathologic overview it provides. It will contribute to improved differential diagnoses and, therefore, improved management of this condition.</p></div>","PeriodicalId":100364,"journal":{"name":"Dentistry Review","volume":"4 3","pages":"Article 100109"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772559624000324/pdfft?md5=25a81d3acb99ee4fed373e31ba28577d&pid=1-s2.0-S2772559624000324-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142168735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immediate Effect of Biomimetic Agents on Caries-Affected Dentin Bond Strength","authors":"Gabriela de Alencar Pinto Magalhes,&nbsp;Rafael Rocha Pacheco,&nbsp;Regina Maria Puppi-Rontani","doi":"10.1016/j.dentre.2024.100142","DOIUrl":"10.1016/j.dentre.2024.100142","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>The purpose of this study was to evaluate the effect of the P11-4 Self-Assembly Peptide and Dentin Matrix Protein 1 (DMP-1) on the microtensile bond strength (μTBS) to Caries-Affected Dentin (CAD) after 24h.</p></div><div><h3>METHODS</h3><p>Dentin slices were sectioned from 48 human third molars, randomly divided into six groups (n = 8): sound dentin [C+]; CAD [C]; CAD with DMP-1 [DMP-1] at 1μg/ml; CAD with P11-4 [P11-4] at 1 μg/ml; CAD with P11-4 [1P11-4] at 1mg/ml; and CAD with a combination of DMP-1 at 1 μg/ml and P11-4 at 0.5 μg/ml [DMP-1/P11-4]. Artificial caries lesions were created on the dentin surfaces of CAD groups (S.mutans, UA159). After selective caries removal, the respective treatments were applied. A 50μL dose of each biomineralization agent was used on the DAC for 5 minutes, and excess removed. Subsequently, 50μL of a supersaturated calcium and phosphate solution was added for 1 minute. The bonding steps followed manufacturer's guidelines (Adper™ Singlebond2, 3M™ Oral Care), and a resin composite layer was added. Specimens were kept in Simulated Body Fluid solution (SBF) for 24 hours at 37ÅãC, simulating pulpal pressure. Sectioned beams (1mm2) were submitted to μTBS test. Group comparisons utilized one-way ANOVA, Tukey post hoc tests, and Dunnett's test against control groups, with pre-set α = 0.05. Chi-square test was conducted on the failure pattern (CI = 95%).</p></div><div><h3>RESULTS</h3><p>C+ presented the highest μTBS values (p&lt;0.05). 1P11-4 and the DMP-1/P11-4 exhibited significantly higher μTBS compared to C- (p&lt;0.05). DMP-1 and P11-4 were similar to C- (p&lt;0.05). The experimental groups showed a decrease in premature failure rates and an increase in mixed failure compared to C- (p &lt; 0.001).</p></div><div><h3>CONCLUSIONS</h3><p>1P11-4 and DMP-1/ P11-4 enhanced bond strength to CAD, with 1P11-4 achieving approximately 60% of μ-TBS values of the C+. Biomimetic analogues improved immediate bond strength between adhesive/composite and CAD.</p></div><div><h3>IMPLICATIONS</h3><p>Minimally Invasive Dentistry advocates for selective caries removal, involving the removal of infected dentin, which is necrotic and unable to remineralize, while preserving the affected dentin at the bottom, which has the potential for remineralization. However, adhesion to affected dentin is often poor. In this study, biomimetic agents were used as a surface treatment to remineralize caries-affected dentin, aiming to improve immediate bond strength.</p></div>","PeriodicalId":100364,"journal":{"name":"Dentistry Review","volume":"4 3","pages":"Article 100142"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772559624000658/pdfft?md5=99ff76b5d287ff05badb5fe66cbe472c&pid=1-s2.0-S2772559624000658-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142168838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictors of standardized in-service examination performance and residency outcomes in a graduate periodontics program.","authors":"Dr. Han S Kim DMD,&nbsp;Thomas Johnson","doi":"10.1016/j.dentre.2024.100129","DOIUrl":"10.1016/j.dentre.2024.100129","url":null,"abstract":"<div><h3>OBJECTIVES</h3><p>The American Academy of Periodontology (AAP) annually offers an extensive multiple-choice in-service examination to graduate periodontics programs across North America. The objectives of this study were to assess the influence of learner- and education-related factors on AAP In-service Examination (AIE) performance and determine whether AIE scores predict residency outcomes.</p></div><div><h3>METHODS</h3><p>AIE scores from 10 periodontics residency classes at a single center were recorded and compared against a panel of learner- and education-related variables using multiple linear regression models. Defined residency outcome measures potentially related to general cognitive ability and knowledge in periodontics and implant dentistry were analyzed against AIE scores using binomial logistic regression.</p></div><div><h3>RESULTS</h3><p>No evaluated learner- or education-related variable was a statistically significant predictor of AIE score in this study sample. Likewise, AIE score was not a statistically significant predictor of any assessed residency outcome.</p></div><div><h3>CONCLUSIONS</h3><p>The AAP has performed a tremendous service to periodontics residents and programs by marshaling the leadership and expertise necessary to offer a professionally constructed assessment instrument. However, in the current study sample, no relationship could be identified between AIE score and any outcome, including first-attempt board certification.</p></div><div><h3>IMPLICATIONS</h3><p>The AAP In-service Committee appears well situated to provide additional leadership focusing on exam implementation, which may enhance AIE value in competency decision making.</p></div>","PeriodicalId":100364,"journal":{"name":"Dentistry Review","volume":"4 3","pages":"Article 100129"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S277255962400052X/pdfft?md5=46ac73c376e836ba76da5005855e8866&pid=1-s2.0-S277255962400052X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}