Decoding Infection and Transmission最新文献

{"title":"Public health significance, seroprevalence and associated risk factors of bovine brucellosis in selected dairy farms of North Shewa zone, Amhara region, Ethiopia","authors":"Gashaw Adane Erkyihun ,&nbsp;Fikru Regassa Gari ,&nbsp;Daniel Demissie Shewie ,&nbsp;Teferi Bentti ,&nbsp;Gezahegne Mamo Kassa","doi":"10.1016/j.dcit.2024.100027","DOIUrl":"10.1016/j.dcit.2024.100027","url":null,"abstract":"<div><h3>Objective</h3><div>Bovine brucellosis is a zoonotic disease that causes economic crises in dairy cattle production and poses public health impact. A cross sectional study was conducted between November 2021 to April 2022 to estimate the seroprevalence of bovine brucellosis, identify associated risk factors with bovine brucellosis and assess the knowledge, attitude and practice of dairy cattle owners on brucellosis.</div></div><div><h3>Methods</h3><div>A total of 426 blood samples of dairy cattle were screened with Rose Bengal Plate Test (RBPT) and positive samples were confirmed by complement fixation test (CFT). Risk factors associated with <em>Brucella</em> seropositivity and knowledge of respondents were assessed and data were analyzed using R software.</div></div><div><h3>Results</h3><div>The overall seroprevalence of bovine brucellosis was 0.47 % (95 % CI: −0.182%-1.121%) and 4.43 % (95 % CI: −1.77 % - 10.47 %) at individual and herd level respectively. Analysis of risk factors using logistic regression indicated buying stock replacement (OR = 33.83, <em>p</em> = 0.000301), abortion history (OR = 5.90, 0.00579) and bull mating service (OR = 6.43, <em>p</em> = 0.01338) were found to be significantly associated with the seropositivity of <em>Brucella</em> infection in the dairy cattle.</div></div><div><h3>Conclusion</h3><div>The presence of serological reactors with significant risk factors indicates previous infection of brucellosis in the cattle population. Hence, culling of <em>Brucella</em> antibody reactor cows, stock replacement through <em>Brucella</em> testing and improving community awareness are recommended.</div></div>","PeriodicalId":100358,"journal":{"name":"Decoding Infection and Transmission","volume":"2 ","pages":"Article 100027"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142653758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global, regional, and national burden of malaria, 1990–2021: Findings from the global burden of disease study 2021","authors":"Shun-Xian Zhang ,&nbsp;Guo-Bing Yang ,&nbsp;Jian Yang ,&nbsp;Fan-Na Wei ,&nbsp;Shan Lv ,&nbsp;Lei Duan ,&nbsp;Li-Guang Tian ,&nbsp;Mu-Xin Chen ,&nbsp;Qin Liu ,&nbsp;Yan Lu ,&nbsp;Ji-Chun Wang ,&nbsp;Shi-Zhu Li ,&nbsp;Xiao-Nong Zhou","doi":"10.1016/j.dcit.2024.100030","DOIUrl":"10.1016/j.dcit.2024.100030","url":null,"abstract":"<div><h3>Background</h3><div>Malaria is an infectious disease transmitted by mosquitoes and caused by Plasmodium parasites. This study analyzes data from the Global Burden of Disease (GBD) Study 2021 to systematically assess global epidemiological trends and the malaria burden from 1990 to 2021, providing scientific insights for malaria prevention and control.</div></div><div><h3>Methods</h3><div>Using GBD 2021 data, we analyzed trends in malaria incidence, prevalence, mortality, and disability-adjusted life years (DALYs), stratified by age, sex, regions, and Socio-demographic Index (SDI). A Bayesian age-period-cohort model was employed to predict the future burden of malaria, while the average annual percent change (AAPC) was calculated to describe overall trends in age-standardized rates (ASR) and counts from 1990 to 2021.</div></div><div><h3>Results</h3><div>From 1990 to 2021, significant decreases were observed in the global age-standardized incidence rate (ASIR, AAPC = −7.25%; 95% <em>CI</em>: −11.68%, −2.81%), prevalence rate (ASPR, AAPC = −14.42%; 95% <em>CI</em>: −15.99%, −12.84%), mortality rate (ASMR, AAPC = −0.06%; 95 % <em>CI</em>: −0.08%, −0.05%), and age-standardized DALY rate (AAPC = −4.86%; 95 % <em>CI</em>: −5.81%, −3.91%). However, no significant changes were found in ASIR for East Asia (AAPC = −0.30%; 95% <em>CI</em>: −0.96%, 0.37%), high-income Asia Pacific (AAPC = −0.03%; 95% <em>CI</em>: −0.07%, 0.01%), and Southern Sub-Saharan Africa (AAPC = 1.09%; 95% <em>CI</em>: −29.12%, 31.30%), while the remaining 14 regions showed a decline. The ASIR, ASPR, ASMR, and age-standardized DALY rates for malaria, as well as total cases and deaths, did not significantly differ between males and females across all age groups. Notably, the highest incidence, prevalence, and mortality rates were observed in children under five years of age. Between 1990 and 2021, the ASIR, ASPR, ASMR, and age-standardized DALY rate for malaria were inversely correlated with SDI levels.</div></div><div><h3>Conclusions</h3><div>Although global malaria burden has steadily declined, malaria remains a serious public health threat, particularly in sub-Saharan Africa and Southeast Asia, especially among children under five. To address the continuing threat of malaria, immediate implementation of stronger and more effective global control strategies is essential.</div></div>","PeriodicalId":100358,"journal":{"name":"Decoding Infection and Transmission","volume":"2 ","pages":"Article 100030"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142701200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing bioinformatics for the development of a promising multi-epitope vaccine against tuberculosis: The ZL9810L vaccine","authors":"Li Zhuang ,&nbsp;Yilu Zhao ,&nbsp;Ling Yang ,&nbsp;Linsheng Li ,&nbsp;Zhaoyang Ye ,&nbsp;Awais Ali ,&nbsp;Yajing An ,&nbsp;Ruizi Ni ,&nbsp;Syed Luqman Ali ,&nbsp;Wenping Gong","doi":"10.1016/j.dcit.2024.100026","DOIUrl":"10.1016/j.dcit.2024.100026","url":null,"abstract":"<div><h3>Background</h3><div>Tuberculosis (TB) is a global infectious disease posing a serious threat to human health, caused by <em>Mycobacterium tuberculosis</em> (MTB) infection. Although vaccination is the most effective way to prevent and control TB, the existing Bacillus Calmette–Guérin (BCG) vaccine exhibits limited protective efficacy in adult pulmonary TB. Therefore, developing a novel TB vaccine is an urgent need.</div></div><div><h3>Methods</h3><div>This study designed a novel multi-epitope vaccine (MEV) to combat MTB infection, utilizing bioinformatics and immunoinformatics approaches. Dominant epitopes of helper T lymphocytes (HTL), cytotoxic T lymphocytes (CTL), and B lymphocytes were selected from antigens of MTB to construct the MEV. To enhance the targeting and immunogenicity of the vaccine, toll like receptor (TLR) agonists and PADRE helper peptides were incorporated into the design. In addition, comprehensive predictions have been made on the physicochemical properties, three-dimensional structure, spatial conformation, and molecular interactions with TLRs of MEVs. These evaluations also extend to the exploration of their immunological characteristics.</div></div><div><h3>Results</h3><div>A novel MEV named ZL9810L was successfully constructed, based on 8 HTL epitopes, 9 CTL epitopes, 10 B lymphocyte epitopes, Toll-like receptor agonists, and auxiliary peptides. Bioinformatics analysis demonstrated that the ZL9810L vaccine has excellent immunogenicity and antigenicity, non-toxicity, and non-sensitization capability, capable of significantly inducing a strong immune response and solubility, with scores of 2.21451, 0.8913, and 0.455 respectively. Moreover, the global population coverage of HLA class I and II allele genes by the ZL9810L vaccine reached 72.89 % and 81.49 %, respectively. Molecular docking analysis indicated good binding capacities of the ZL9810L vaccine to TLR2 and TLR4 receptors, with binding energies of −1028.5 kcal/mol and −1018.8 kcal/mol respectively. Immune simulation predicted that the vaccine could effectively activate innate and adaptive immune cells, including NK cells, macrophages, B lymphocytes, and T lymphocytes.</div></div><div><h3>Conclusion</h3><div>As a candidate for a novel TB vaccine, the ZL9810L vaccine exhibits significant immunogenicity and antigenicity, with no toxicity or allergenicity. The ZL9810L vaccine designed in this study provides a new vaccine candidate for TB control and prevention.</div></div>","PeriodicalId":100358,"journal":{"name":"Decoding Infection and Transmission","volume":"2 ","pages":"Article 100026"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142573511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efforts of rabies elimination with a One Health approach and SARE tools in Ethiopia","authors":"Gashaw Adane Erkyihun ,&nbsp;Sisay Getachew Deressa ,&nbsp;Wubishet Zewdie Wakene ,&nbsp;Fikru Regassa Gari","doi":"10.1016/j.dcit.2024.100028","DOIUrl":"10.1016/j.dcit.2024.100028","url":null,"abstract":"<div><div>Rabies is a viral zoonosis which affects nearly every region of the world. Once symptoms appear, the disease is 100 % fatal, although it is 100 % preventable through vaccination. Human rabies is typically caused by virus transmitted from the bite of an infected dog. Rabies results in significant economic costs and human fatalities, with children being disproportionately affected. Numerous countries have embraced a global goal to eliminate dog-mediated human rabies by 2030. In line with this, Ethiopia has integrated a global prevention and elimination strategy into its national program, and is implementing various interventions. During 2022 and 2023, the Ministry of Agriculture, in partnership with One Health collaborators, vaccinated over 350,000 domestic dogs. Documenting these efforts and best practices is essential for sharing ongoing control and elimination experiences with other countries. This review outlines the burden and impact of rabies, the current status of the Ethiopian 2030 rabies control and elimination program, and its achievements and challenges. The review also highlights the current state of stakeholder collaboration and coordination efforts.</div></div>","PeriodicalId":100358,"journal":{"name":"Decoding Infection and Transmission","volume":"2 ","pages":"Article 100028"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142653757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent malaria outbreak in the USA: Risk of and response to malaria reintroduction in non-endemic regions","authors":"Liwang Cui,&nbsp;Kami Kim","doi":"10.1016/j.dcit.2024.100018","DOIUrl":"https://doi.org/10.1016/j.dcit.2024.100018","url":null,"abstract":"<div><p>The emergence of locally transmitted <em>Plasmodium vivax</em> malaria in Florida from May to July 2023 underscores the persistent threat of malaria reintroduction in non-endemic regions. The increasing instances of imported malaria associated with international travel, alongside the existence of competent local vectors, emphasize the critical need for public health authorities in non-endemic countries to remain vigilant and well-prepared to sustain a malaria-free status.</p></div>","PeriodicalId":100358,"journal":{"name":"Decoding Infection and Transmission","volume":"2 ","pages":"Article 100018"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949924024000028/pdfft?md5=a01a4f9c6ad56e8750976a379ea660a6&pid=1-s2.0-S2949924024000028-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140320408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress of exosomes in diagnosis of tuberculosis","authors":"Yuheng Liu ,&nbsp;Yao Liao ,&nbsp;Ji Wu ,&nbsp;ZiFeng Zhu ,&nbsp;DingHao Li ,&nbsp;Lifu Wang","doi":"10.1016/j.dcit.2024.100020","DOIUrl":"10.1016/j.dcit.2024.100020","url":null,"abstract":"<div><div>Tuberculosis (TB) is a globally prevalent infectious disease caused by <em>Mycobacterium tuberculosis</em>. A majority of TB patients develop a latent infection, making disease diagnosis more challenging. However, current diagnostic methods are not able to accurately identify TB patients and those with latent TB. Therefore, there is a need to develop new methods for the accurate diagnosis of TB. Exosomes, which are extracellular vesicles with a diameter of 40∼160 nm, contain a variety of proteins, lipids, nucleic acids, and play a crucial role in intercellular communication. Recent research suggests that exosomes can be used for disease diagnosis, and exosome-derived substances such as proteins, miRNAs show great potential as disease biomarkers. This study aims to review the progress of exosomes in diagnosing TB and proposes new ideas for exploring exosomal biomarkers of TB.</div></div>","PeriodicalId":100358,"journal":{"name":"Decoding Infection and Transmission","volume":"2 ","pages":"Article 100020"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949924024000041/pdfft?md5=2794c105989f0210e6c44780cb75d5cd&pid=1-s2.0-S2949924024000041-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The prevalence, diagnosis, and dissemination of mcr-1 in colistin resistance: Progress and challenge","authors":"Jun Feng ,&nbsp;Zhen Xu ,&nbsp;Yuan Zhuang ,&nbsp;Mingxiang Liu ,&nbsp;Jiayuan Luo ,&nbsp;Yitong Wu ,&nbsp;Yong Chen ,&nbsp;Min Chen","doi":"10.1016/j.dcit.2023.100007","DOIUrl":"https://doi.org/10.1016/j.dcit.2023.100007","url":null,"abstract":"<div><p>Considered as the last defense line against Gram-negative bacteria, colistin is particularly effective against multiple drug-resistant bacteria such as multi-drug resistance (MDR) and carbapenem-resistant <em>Enterobacteriaceae</em> (CRE). However, since the plasmid-mediated mobile colistin resistance-1 (<em>mcr-1</em>) was first reported in China in 2015, it has been widely detected from humans, animals, and the environment across 47 countries/regions worldwide by 2019, which has attracted the attention of the World Health Organization and global government departments, who formulated emergency strategies and interventions to contain its emergence and spread. At present, three kinds of colistin for clinical injection have been adopted in clinic in China. Due to drug pressure screening, the risk with Gram-negative bacteria developing resistance to colistin may corresponding increase. Therefore, rapid, accurate and sensitive detection methods are particularly needed. This review summarizes the prevalence of <em>mcr-1</em>, in addition to a diverse assortment of different detection techniques of colistin resistance, and analyzes the application scenarios, advantages, and challenges of different technologies, in order to provide a scientific evidence-based proof for the rapid detection of colistin resistance.</p></div>","PeriodicalId":100358,"journal":{"name":"Decoding Infection and Transmission","volume":"1 ","pages":"Article 100007"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949924023000071/pdfft?md5=61c26d50a9ff064c3550394341a47742&pid=1-s2.0-S2949924023000071-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138570696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of directly acting antiviral drugs in HCV patients with HIV in liver transplantation: A meta-analysis","authors":"Tian Zeng ,&nbsp;Peng Huang ,&nbsp;Weilong Tan ,&nbsp;Zepei Feng ,&nbsp;Jianguo Shao ,&nbsp;Xueshan Xia ,&nbsp;Chao Shen ,&nbsp;Liqin Qian ,&nbsp;Bingqing Wang ,&nbsp;Zhengjie Li ,&nbsp;Chuanlong Zhu ,&nbsp;Yun Zhang ,&nbsp;Ming Yue","doi":"10.1016/j.dcit.2023.100005","DOIUrl":"https://doi.org/10.1016/j.dcit.2023.100005","url":null,"abstract":"<div><h3>Background</h3><p>HCV/HIV co-infections were initially a contentious consideration for liver transplantation, primarily due to their suboptimal response to interferon-based treatments and unfavorable post-transplantation outcomes. The potential concern in this patient group arises from drug–drug interactions between DAAs and ARVs, with data on the effectiveness and safety of DAAs in this demographic primarily derived from isolated case studies. This extensive review assesses the safety and efficacy of DAAs in liver transplants for individuals with concurrent HIV and HCV infections.</p></div><div><h3>Methods</h3><p>Conducting a systematic search across multiple databases until April 2023, our primary focus was the evaluation of outcomes, specifically the proportion of sustained virologic responses at week 12 following therapy (SVR12). To gauge publication bias, we scrutinized funnel plots and conducted Egger tests.</p></div><div><h3>Results</h3><p>Nine studies encompassed a participant pool of 269 individuals, with a statistical estimate of SVR12 at 92% (95% CI: 88–95). Subgroup analysis showed that the ratio of binding SVR12 of genotype (GT) 1a was 97% (95% CI: 87–100), while that of GT3 was 100% (95% CI: 92–100); 88% (95%CI: 80–95) for pre-transplant treatments; and 95% (95%CI: 91–99) for post-transplant treatments subgroup. A total of 8 patients died during SVR12 completion while 269 had a survival rate of 99% (95% CI 97–100). After one year of follow-up, four studies recorded a 98% survival rate (95% CI 94–100). Egger's test did not reveal any publication bias.</p></div><div><h3>Conclusion</h3><p>Administration of DAAs during liver transplantation for HCV patients with HIV infections has a high efficacy and safety. Early consideration of HCV therapy should be the goal for all liver transplant recipients.</p></div>","PeriodicalId":100358,"journal":{"name":"Decoding Infection and Transmission","volume":"1 ","pages":"Article 100005"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949924023000058/pdfft?md5=d05d48f565fa7cbefb97fc40c6aafd08&pid=1-s2.0-S2949924023000058-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134656657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new scoring system to predict tuberculosis among South-Tunisian patients with lymphocytic meningitis","authors":"Houda Ben Ayed ,&nbsp;Makram Koubaa ,&nbsp;Sirine Chtourou ,&nbsp;Khaoula Rekik ,&nbsp;Fatma Hammami ,&nbsp;Chakib Marrekchi ,&nbsp;Jamel Damak ,&nbsp;Mounir Ben Jemaa","doi":"10.1016/j.dcit.2023.100003","DOIUrl":"https://doi.org/10.1016/j.dcit.2023.100003","url":null,"abstract":"<div><h3>Background</h3><p>Death and poor outcome due to Tuberculous meningitis (TBM) is greatly influenced by the delayed treatment initiation, which often occurs in lymphocytic meningitis (LM). This study aimed to propose an easy-to-use clinical prediction score that can accurately diagnose TBM among LM patients.</p></div><div><h3>Methods</h3><p>It was a prospective cohort study including all patients with LM hospitalized at the infectious diseases department in Southern Tunisia from 2008 to 2022 were included.</p></div><div><h3>Results</h3><p>Among 290 LM patients, 105 cases (36.2%) had TBM. By multivariate analysis, age ≥60 years, rural origin, symptom duration ≥5days, thrill, deteriorated general conditions, a hospital stay ≥ 10 days, complicated forms, blood white cells count &lt;4000/mm3, CSF/blood glucose ratio&lt;0.5, hydrocephalus and arachnoiditis were independent factors of TBM. Individual diagnosis indexes from 1 to 3 points were attributed to these factors to produce an overall score ranging from 0 to 16 points. At a cut-off of 7, the predictive score had a receiver operating characteristic (ROC) area of 0.94, a sensitivity, a specificity and a diagnosis accuracy of respectively 81%, 91.4%, and 87.5%.</p></div><div><h3>Conclusions</h3><p>This original study proposed a novel scoring system that can reliably identify patients with TBM at hospital admission and could be easily used in clinical practice.</p></div>","PeriodicalId":100358,"journal":{"name":"Decoding Infection and Transmission","volume":"1 ","pages":"Article 100003"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49727505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Observation of intestinal flora diversity with the parasites infection process in a nonlethal malaria model of BALB/c mice induced by Plasmodium yoelii 17XNL strain","authors":"Wei Guan ,&nbsp;Daoxiu Xu ,&nbsp;Shuguo Yang ,&nbsp;Yanqing Zhao ,&nbsp;Yiting Xie ,&nbsp;Min Lin ,&nbsp;Yaqun Liu ,&nbsp;Yuzhong Zheng ,&nbsp;Jian Li","doi":"10.1016/j.dcit.2023.100004","DOIUrl":"https://doi.org/10.1016/j.dcit.2023.100004","url":null,"abstract":"<div><p>Gut flora plays an important role in infectious diseases such as malaria, but few studies are conducted in the associated filed of murine malaria infected with <em>Plasmodium yoelii</em> 17XNL (Py 17XNL). In this study, the alteration of intestinal flora composition in BALB/c mice infected with Py 17XNL was detected. The kinetics of parasitemia was assessed at 1, 6, 9, 15, 25, and 28 days postinfection (dpi). The survival percentage was calculated to assess the mortality. The parasitemia reached the highest degree at 9 dpi and almost eliminated at 25 dpi. Interestingly, the morbidity was severe at 9 dpi, but it almost recovered at 28 dpi. Regarding the gut microbiota, the gut microbiota from BALB/c mice was examined by sequencing the V4 region of the 16S rRNA through the Illumina MiSeq platform. The results revealed the apparent variation of operational taxonomic unit (OTU) clustering, relative abundance of microbial composition, alpha and beta diversity among the seven groups, with an increase of the alpha diversity and a decrease of beta diversity at 9 and 15 dpi, which recovered at 28 dpi. The LEfSe analysis selected potential biomarkers at genus and species levels, such as <em>Lactobacillus gasseri</em> in Py9 and Py15 groups, <em>Pseudomonas veronii</em> in Py25 group, and <em>Lactobacillus intestinalis</em> and <em>Psychrobacter</em> in Py28 group. This study offers a new insight for investigating the effect of gut microbiota on the occurrence and development of malaria, and also provides new ideas for the treatment and prevention of malaria.</p></div>","PeriodicalId":100358,"journal":{"name":"Decoding Infection and Transmission","volume":"1 ","pages":"Article 100004"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49765941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}