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OMICS strategies: Revealing the enigma of salinity tolerance in mangroves OMICS 战略:揭示红树林耐受 SA- LINITY 的 ENIGMA 特性
Crop Design Pub Date : 2024-05-01 DOI: 10.1016/j.cropd.2024.100052
K. Henna Parveen, Jumana Muhammed, V.K. Sneha, P. Busheera, Anu Augustine
{"title":"OMICS strategies: Revealing the enigma of salinity tolerance in mangroves","authors":"K. Henna Parveen,&nbsp;Jumana Muhammed,&nbsp;V.K. Sneha,&nbsp;P. Busheera,&nbsp;Anu Augustine","doi":"10.1016/j.cropd.2024.100052","DOIUrl":"10.1016/j.cropd.2024.100052","url":null,"abstract":"<div><p>Salinity is a significant challenge for agriculture, negatively impacting soil health and crop yields worldwide. Coping with salinity stress is intricate due to its multifaceted nature, making it challenging to fully grasp. Mangroves, recognized for their salt tolerance, thrive in diverse salinity levels, spanning from freshwater to seawater. They play a vital role in coastal ecosystems, thriving in areas where many other plants struggle. For a thorough knowledge of the salinity stress signaling and tolerance mechanism in mangroves, a variety of “omics” techniques have been explored. Recent research has illuminated crucial pathways, transcription factors, microRNAs, and signaling components in mangroves exposed to salty conditions. This knowledge holds promise for developing salt-tolerant crop plants through genetic modification techniques, which can help address the increasing issue of soil salinity. Our review encompasses genomics and transcriptomics studies that identify crucial genes and pathways in mangroves' response to salinity. Since the transcriptome lacks a direct correlation with the protein expression dynamics, we have also emphasized mangrove proteomics and metabolomics studies. The review also outlines the different strategies that can be used to enhance the salinity tolerance of crops using mangroves as models.</p></div>","PeriodicalId":100341,"journal":{"name":"Crop Design","volume":"3 2","pages":"Article 100052"},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772899424000016/pdfft?md5=22d6ee653fdea040d2e86568bf24bab8&pid=1-s2.0-S2772899424000016-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140468290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular docking insights into nuclear factor Y (NF-Y) transcription factor and pyrabactin resistance 1 (PYL) receptor proteins reveal abiotic stress regulation in finger millet 核因子 Y(NF-Y)转录因子和吡拉菌素抗性 1(PYL)受体蛋白的分子对接研究揭示了指状黍中的非生物胁迫调控机制
Crop Design Pub Date : 2024-02-01 DOI: 10.1016/j.cropd.2023.100051
Varsha Rani , Vinay Kumar Singh , D.C. Joshi , Rajesh Singh , Dinesh Yadav
{"title":"Molecular docking insights into nuclear factor Y (NF-Y) transcription factor and pyrabactin resistance 1 (PYL) receptor proteins reveal abiotic stress regulation in finger millet","authors":"Varsha Rani ,&nbsp;Vinay Kumar Singh ,&nbsp;D.C. Joshi ,&nbsp;Rajesh Singh ,&nbsp;Dinesh Yadav","doi":"10.1016/j.cropd.2023.100051","DOIUrl":"10.1016/j.cropd.2023.100051","url":null,"abstract":"<div><p>Finger Millet (<em>Eleusine coracana -</em> (L.) Gaertn), is an important nutraceutical crop with the potential for imparting food and nutritional security. These plants have a comparatively higher tolerance for several abiotic stresses like drought, salinity, and heat. Several players including Transcription Factor (TF) like Nuclear Factor Y (NF-Y) might be associated with this enhanced level of tolerance. Further, it is unclear how phytohormones like Abscisic acid (ABA) regulate the expression of NF-Y, whether in ABA-dependent or ABA-Independent pathway. The interaction of PYL (Pyrabactin resistance1-like) receptor proteins with Nuclear Factor Y (NF-Y) Transcription Factor in the presence of phytohormones like abscisic acid (ABA) provides one insight related to the enhanced tolerance towards abiotic stresses under ABA-dependent signaling in finger millet crop. A total of three PYL receptors of finger millet designated as EcPYL1, EcPYL5, and EcPYL9 were retrieved in the finger millet genome. These receptors were modeled through the SWISS-MODEL using templates 5gwo and 3wg8 and docked with ABA. The best-docked protein-ligand complex PYL5-ABA (binding energy ΔG ​= ​-8.8 kcal mol<sup>-1</sup>) was found to be most stable at the 50ns MD simulation study. Further protein-protein interaction between PYL5 and NF-YA2/B3/C1 sub-family members showed a good interaction. This clearly indicates the possibility of the NF-Y-PYL module in the ABA transduction pathway, which performs a crucial role in the expression of stress-responsive genes. These studies reveal the intricate relationship between the ABA, PYL receptors of finger millet, and NF-Y transcription factor in regulating the stress-responsive genes and provide an insight into the abiotic stress tolerance mechanisms, which can be targeted for crop improvement.</p></div>","PeriodicalId":100341,"journal":{"name":"Crop Design","volume":"3 1","pages":"Article 100051"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772899423000290/pdfft?md5=377fe0f6fe5b520a892715bbc5d32b47&pid=1-s2.0-S2772899423000290-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139127488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genomic exploration and functional insights into the diversification of the Snf2 gene family in subgenomes of Triticum aestivum 小麦亚基因组中 Snf2 基因家族多样化的基因组探索和功能研究
Crop Design Pub Date : 2023-12-14 DOI: 10.1016/j.cropd.2023.100050
Muhammad Fahad , Chuanjia Liu , Yuxin Shen , Muhammad Sajid , Liang Wu
{"title":"Genomic exploration and functional insights into the diversification of the Snf2 gene family in subgenomes of Triticum aestivum","authors":"Muhammad Fahad ,&nbsp;Chuanjia Liu ,&nbsp;Yuxin Shen ,&nbsp;Muhammad Sajid ,&nbsp;Liang Wu","doi":"10.1016/j.cropd.2023.100050","DOIUrl":"10.1016/j.cropd.2023.100050","url":null,"abstract":"<div><p>Sucrose nonfermenting 2 (Snf2) family proteins function as the ATP-dependent catalytic engines of chromatin remodeling complexes, which harness ATP hydrolysis energy to alter chromatin structure and nucleosome positioning, enabling regulatory factor access to DNA. Plant genomes contain numerous Snf2 family proteins, several of which have been demonstrated to act as key developmental regulators at different stages in model plants like Arabidopsis and rice. Despite their vital roles, the <em>Snf2</em> genes in <em>Triticum aestivum</em> remain largely uncharacterized. Here, we report the identification of 112 wheat <em>Snf2</em> genes that were unevenly distributed across the 21 chromosomes, with 40 genes on the A subgenome, 33 on the B subgenome, and 39 on the D subgenome, and phylogenetically classified these <em>Snf2</em> genes into 18 subfamilies related to the 6 Snf2 groups in Arabidopsis. Evolutionary analysis revealed that purifying selection has largely driven the evolution of <em>Snf2</em> genes, acting as the primary selective force shaping the <em>Snf2</em> gene family in wheat, while segmental duplications have served as the main mechanism for expanding the gene family. All identified Snf2 proteins contained at least one Helicase_C and SNF2_N domain among 10 conserved domains, and their gene structures consisted of 3–38 exons. Tissue-specific expression analysis uncovered distinct expression patterns among <em>Snf2</em> gene family members, including some with enhanced reproductive tissue expression, while analysis under various abiotic and biotic stresses revealed differential regulation of specific family members in response to these conditions. Overall, these systematic analyses including identification, evolutionary relationships, and expression profiling provide valuable insights into the wheat Snf2 family while establishing a genomic framework to elucidate Snf2 functional roles in wheat growth, development, and stress responses.</p></div>","PeriodicalId":100341,"journal":{"name":"Crop Design","volume":"3 1","pages":"Article 100050"},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772899423000289/pdfft?md5=4b4ddd09e117ea839bd12764824bf75f&pid=1-s2.0-S2772899423000289-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139018768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Understanding the molecular breeding and omics approaches for finger millet (Eleusine coracana L.) improvement towards a global sustainable nutritional security 了解分子育种和分子生物学方法,改良小米(Eleusine coracana L.),实现全球可持续营养安全
Crop Design Pub Date : 2023-12-09 DOI: 10.1016/j.cropd.2023.100049
Salma Kayastha , Jyoti Prakash Sahoo , Manaswini Mahapatra , Narayan Panda
{"title":"Understanding the molecular breeding and omics approaches for finger millet (Eleusine coracana L.) improvement towards a global sustainable nutritional security","authors":"Salma Kayastha ,&nbsp;Jyoti Prakash Sahoo ,&nbsp;Manaswini Mahapatra ,&nbsp;Narayan Panda","doi":"10.1016/j.cropd.2023.100049","DOIUrl":"10.1016/j.cropd.2023.100049","url":null,"abstract":"<div><p>Finger millet (<em>Eleusine coracana</em> L.) has gained notable interest in recent times for its capacity to tackle global challenges related to nutritional security and sustainability. This review delves into the critical issue of global nutritional security by focusing on Finger millet, as an essential staple crop known for its rich nutraceutical content. However, due to the expanding global population and the imperative for sustainable food sources, there is an urgent need to elevate the nutritional content of crops such as Finger millet. This review also utilizes molecular breeding and omics methodologies, encompassing genomics, transcriptomics, proteomics, and metabolomics, to thoroughly examine the molecular dimensions involved in enhancing the nutraceutical content and other crucial agronomic traits in Finger millet. These cutting-edge techniques provide insights into the genetic makeup and expression of key genes related to nutrient accumulation and distribution during grain development, as well as for other important agronomic traits. Moreover, this review also emphasizes the importance of sustainable agricultural practices to ensure the long-term availability of nutrient-rich crops like Finger millet. However, by harnessing the power of genetics and advanced analytical genomic tools, researchers can pave the way for the development of Finger millet varieties, contributing to a more sustainable and nutritious food security for the growing world population.</p></div>","PeriodicalId":100341,"journal":{"name":"Crop Design","volume":"3 1","pages":"Article 100049"},"PeriodicalIF":0.0,"publicationDate":"2023-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772899423000277/pdfft?md5=ef3fc16e735afad0d2f10e2940258d96&pid=1-s2.0-S2772899423000277-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138625719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Controlled Environment Ecosystem: A plant growth system to combat climate change through soilless culture 可控环境生态系统:通过无土栽培应对气候变化的植物生长系统
Crop Design Pub Date : 2023-11-18 DOI: 10.1016/j.cropd.2023.100044
Avinash Sharma , Mainu Hazarika , Punabati Heisnam , Himanshu Pandey , V.S. Devadas , Mannu Wangsu
{"title":"Controlled Environment Ecosystem: A plant growth system to combat climate change through soilless culture","authors":"Avinash Sharma ,&nbsp;Mainu Hazarika ,&nbsp;Punabati Heisnam ,&nbsp;Himanshu Pandey ,&nbsp;V.S. Devadas ,&nbsp;Mannu Wangsu","doi":"10.1016/j.cropd.2023.100044","DOIUrl":"10.1016/j.cropd.2023.100044","url":null,"abstract":"<div><p>The variations in climatic factors can lead to morphological changes like male sterility, self-incompatibility, embryo abortions, poor seed setting, low nutrient content, growth retardation, yield loss and even crop failure of agricultural or horticultural crops under open land ecosystem. The traditional agricultural systems involve using a large quantity of water on a large arable land, along with a lot of agro chemicals, which can lead to leaching of nutrients and chemical residues into the soil and water bodies, insect competition, weed emergence, or soil erosion. The controlled environment plant growth chamber reserves 80 ​% land use, 90 ​% water use and runs off or translocates nutrients more efficiently than traditional agriculture. The controlled environment plant growth chamber utilizes standard nutrient diffusion for plant growth and regulates stable nutrient use efficiency, water use efficiency, photosynthetic assimilation product, metabolic use efficiency, climatic factors, greenhouse gases emission, carbon sequestration, carbon foot print with the mechanistic model with machine learning examines critical or non critical levels, dynamics and influences of water, climate and nutrient in controlled environment system. The controlled environment plant growth chamber enables production of outstanding physical and chemical quality enriched plants. The controlled environment witnesses the combat against climate change and the restoration or conservation of natural resources, as well as the generation of research, employment, and industrial development. More comparative investigations are required to understand and justify climate combating with major plants in controlled environment ecosystem and natural environment ecosystem with or without soil.</p></div>","PeriodicalId":100341,"journal":{"name":"Crop Design","volume":"3 1","pages":"Article 100044"},"PeriodicalIF":0.0,"publicationDate":"2023-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772899423000228/pdfft?md5=b9597ed4041e88364ab3fb2038fa6c73&pid=1-s2.0-S2772899423000228-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139291324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aggregation of chromosome axis proteins on the chromatin and in the nucleoplasm of Brassica oleracea meiocytes 甘蓝减数细胞染色质和核质中染色体轴蛋白的聚集
Crop Design Pub Date : 2023-11-01 DOI: 10.1016/j.cropd.2023.100038
Wenbo Shan, Christophe Lambing
{"title":"Aggregation of chromosome axis proteins on the chromatin and in the nucleoplasm of Brassica oleracea meiocytes","authors":"Wenbo Shan,&nbsp;Christophe Lambing","doi":"10.1016/j.cropd.2023.100038","DOIUrl":"10.1016/j.cropd.2023.100038","url":null,"abstract":"<div><p>Meiotic recombination is essential for the generation of genetic diversity in natural and breeding context. The chromosome axis comprises cohesin, HORMA-domain containing proteins and coiled coil proteins and is crucial for the establishment of meiotic recombination. These proteins form a complex during meiosis of <em>Brassica oleracea</em> but information about their respective localisation and dynamic on meiotic chromosomes remain sparse. Our study reveals that the HORMA-protein ASY1 aggregates on the chromatin and forms domains of high and low abundances. The regions enriched for ASY1 are also highly enriched for the axis proteins ASY3, SMC3 and SCC3, although to varying degrees between leptotene and pachytene stages. At later stages, when most DNA double strand breaks are repaired and the chromosome axis disassemble, ASY1, ASY3, SCC3 and SMC3 co-localise and form large aggregates on the discontinuous axis structures. As the axis structures reduce in length, we found that all four axis proteins relocalise in the nucleoplasm and further aggregates. Moreover, we found that ZYP1, the transverse filament of the synaptonemal complex, forms numerous chromosomic aggregates that are sometimes associated with MLH1 and can form ectopic synaptic interactions. Overall, our study indicates that axis proteins have a high propensity to aggregate. This property is important for assembling the chromosome axis but the association of axis proteins with the chromatin must be tightly regulated to limit polycomplex formation.</p></div>","PeriodicalId":100341,"journal":{"name":"Crop Design","volume":"2 2","pages":"Article 100038"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772899423000162/pdfft?md5=8869f99de0ef25c531c8a25a9aa32271&pid=1-s2.0-S2772899423000162-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76553371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent progresses in plant single-cell transcriptomics 植物单细胞转录组学研究进展
Crop Design Pub Date : 2023-11-01 DOI: 10.1016/j.cropd.2023.100041
Dihuai Zheng , Jiwei Xu , Yaqian Lu , Hongyu Chen , Qinjie Chu , Longjiang Fan
{"title":"Recent progresses in plant single-cell transcriptomics","authors":"Dihuai Zheng ,&nbsp;Jiwei Xu ,&nbsp;Yaqian Lu ,&nbsp;Hongyu Chen ,&nbsp;Qinjie Chu ,&nbsp;Longjiang Fan","doi":"10.1016/j.cropd.2023.100041","DOIUrl":"10.1016/j.cropd.2023.100041","url":null,"abstract":"<div><p>High-throughput sequencing technologies at single-cell resolution have great potential to reveal a new landscape of plant cells. Single-cell/nucleus RNA (scRNA/snRNA), single-cell/nucleus assay for transposase accessible chromatin (scATAC/snATAC) and spatial transcriptome sequencing have been applied in multiple plant tissues. Consequently, a significant increase in publications on plant single-cell transcriptomics was seen in the recent two years. In this review, we will summarize the advantages and weaknesses of these single-cell sequencing approaches, offer a glimpse of their developments in cell biology, bioinformatic tools and databases in the latest two years.</p></div>","PeriodicalId":100341,"journal":{"name":"Crop Design","volume":"2 2","pages":"Article 100041"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772899423000198/pdfft?md5=571167657c9e3bfdceb6a0720390134d&pid=1-s2.0-S2772899423000198-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87953820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nanotechnology: A promising field in enhancing abiotic stress tolerance in plants 纳米技术:提高植物非生物抗逆性的一个有前途的领域
Crop Design Pub Date : 2023-11-01 DOI: 10.1016/j.cropd.2023.100037
Mawuli K. Azameti, Abdul-Wahab M. Imoro
{"title":"Nanotechnology: A promising field in enhancing abiotic stress tolerance in plants","authors":"Mawuli K. Azameti,&nbsp;Abdul-Wahab M. Imoro","doi":"10.1016/j.cropd.2023.100037","DOIUrl":"10.1016/j.cropd.2023.100037","url":null,"abstract":"<div><p>Heavy metals, drought, salinity, cold stress, and heat stress are some of the main abiotic stresses that adversely affect plant growth and crop productivity generally. For crop production systems to be sustainable in the face of abiotic environmental constraints, effective strategies must be used in conjunction with technological advancement to achieve this goal. In recent times, the emergence of nanotechnology as an intriguing field of study with application potential in the field of agriculture needs to be given the necessary attention to address some of the abiotic stresses. Due to their environmental friendliness, affordability, special physicochemical properties, and increased plant productivity, the use of nanoparticles (NPs) as nano fertilizers has enormous potential and much interest has developed in the field in recent times. Abiotic stress management involves NPs, according to several studies. In order to create a practical and environmentally responsible plan for the long-term sustainability of agriculture, this review focuses on the effects of abiotic stress on plants, synthesis, and the roles of NPs in managing abiotic stresses, and future prospects.</p></div>","PeriodicalId":100341,"journal":{"name":"Crop Design","volume":"2 2","pages":"Article 100037"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772899423000150/pdfft?md5=3337ca41800ad04c948740e2fda3d34e&pid=1-s2.0-S2772899423000150-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72578338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Phenotypic and molecular evaluation of Arachis hypogaea L. against foliar fungal diseases 花生抗叶面真菌病害的表型和分子评价
Crop Design Pub Date : 2023-11-01 DOI: 10.1016/j.cropd.2023.100036
Sunil Yadav , Sushma Tiwari , Manoj Kumar Tripathi , Neha Gupta , Sangeeta Singh , Niraj Tripathi
{"title":"Phenotypic and molecular evaluation of Arachis hypogaea L. against foliar fungal diseases","authors":"Sunil Yadav ,&nbsp;Sushma Tiwari ,&nbsp;Manoj Kumar Tripathi ,&nbsp;Neha Gupta ,&nbsp;Sangeeta Singh ,&nbsp;Niraj Tripathi","doi":"10.1016/j.cropd.2023.100036","DOIUrl":"10.1016/j.cropd.2023.100036","url":null,"abstract":"<div><p>Groundnut improvement tasks are generally engrossed to breed foliar fungal disease-resistant varieties. Early and late leaf spot diseases of groundnuts cause significant loss in yield and quality of groundnut seeds. Simple Sequence Repeat (SSR) markers represent high polymorphic amplification, while Inter-simple sequence (ISSR) is being used widely for diversity assessment due to their large size and reproducible results. The current investigation was carried out with 96 groundnut genotypes for morpho-physiological characterization, disease indexing for early and late leaf spot diseases under field conditions and by employing SSR and ISSR markers for diversity assessment. In correlation analysis, kernel weight was found positively and highly significantly correlated with harvest index and pod weight (r ​= ​0.906) at 1% level of significance. While, pod weight is negatively correlated with early leaf spot at 30 days (r ​= ​-0.401) and at 45 days (r ​= ​-0.410), late leaf spot at 70 days (r ​= ​-0.342) and at 85 days (r ​= ​-0.309) at 1% significant level. With the use of SSR markers, a total 16 alleles were recognized with a mean of 3.20 alleles per locus. The gene multiplicity differed from 0.6074 to 0.6491 for the markers Seq5D05 and PM137 respectively with an average of 0.6289. A total 18 alleles were produced by ISSR markers with an average of 3.6 alleles per locus. In our study, the gene diversity varied from 0.5877 to 0.6625 for the markers ISSR3 and ISSR21 respectively with an average of 0.6164. Diverse group of groundnut germplasm has been identified on the basis of SSR and ISSR markers along with morphological characterization.</p></div>","PeriodicalId":100341,"journal":{"name":"Crop Design","volume":"2 2","pages":"Article 100036"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772899423000149/pdfft?md5=7371306fa461810cb3e988ed68db7849&pid=1-s2.0-S2772899423000149-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75724266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multivariate analysis of garlic (Allium sativum L.) germplasm in response to cold tolerance regimes 大蒜(Allium sativum L.)种质对耐寒性响应的多变量分析
Crop Design Pub Date : 2023-11-01 DOI: 10.1016/j.cropd.2023.100042
Jalil Ahmad , Haiping Wang , Jiangping Song , Shamim Umer , Xiaohui Zhang , Wenlong Yang , Xixiang Li
{"title":"Multivariate analysis of garlic (Allium sativum L.) germplasm in response to cold tolerance regimes","authors":"Jalil Ahmad ,&nbsp;Haiping Wang ,&nbsp;Jiangping Song ,&nbsp;Shamim Umer ,&nbsp;Xiaohui Zhang ,&nbsp;Wenlong Yang ,&nbsp;Xixiang Li","doi":"10.1016/j.cropd.2023.100042","DOIUrl":"https://doi.org/10.1016/j.cropd.2023.100042","url":null,"abstract":"<div><p>Low temperature is a major environmental constraint that limits crop productivity. In this investigation, 256 diverse garlic germplasm were tested for their cold tolerance at the seedling stage by being exposed to natural low-temperature stress −10∼-15 ​°C for the lowest at night for eight days. Several plant development indicators, as well as the cold index (CI), were studied. The findings showed a significant range of CI among these accessions, ranging from 16.98 to 70.38. All germplasms were divided into five groups according to their CI and different grades of tolerance to low-temperature stress. Four highly tolerant and eight low temperature-tolerant germplasm were screened out. Multivariate analysis of the acquired phenomic data using principal component analysis (PCA) addressed sufficient variability, i.e., 70.5% revealed a significant influence of low-temperature stress on growth and bulb attributes. PCA and cluster analysis classified accessions into three groups representing high diversity, providing feasibility for their use in breeding programs. In many phenotypic variables, different germplasm responded differently to low-temperature stress. Furthermore, an exceptionally significantly negative correlation was observed between CI and agronomic traits (PH, LL, LW, RHL) and initiation of bulb traits (Bulb height, width, weight)<em>.</em> This study provides a sustainable solution and useful resources for the garlic low temperature tolerant genetic enhancement.</p></div>","PeriodicalId":100341,"journal":{"name":"Crop Design","volume":"2 2","pages":"Article 100042"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772899423000204/pdfft?md5=ccad90c74f344e0e87074095f6c0efcd&pid=1-s2.0-S2772899423000204-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92059884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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