Phenotypic and molecular evaluation of Arachis hypogaea L. against foliar fungal diseases

Crop Design Pub Date : 2023-11-01 DOI:10.1016/j.cropd.2023.100036

Sunil Yadav , Sushma Tiwari , Manoj Kumar Tripathi , Neha Gupta , Sangeeta Singh , Niraj Tripathi

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Abstract

Groundnut improvement tasks are generally engrossed to breed foliar fungal disease-resistant varieties. Early and late leaf spot diseases of groundnuts cause significant loss in yield and quality of groundnut seeds. Simple Sequence Repeat (SSR) markers represent high polymorphic amplification, while Inter-simple sequence (ISSR) is being used widely for diversity assessment due to their large size and reproducible results. The current investigation was carried out with 96 groundnut genotypes for morpho-physiological characterization, disease indexing for early and late leaf spot diseases under field conditions and by employing SSR and ISSR markers for diversity assessment. In correlation analysis, kernel weight was found positively and highly significantly correlated with harvest index and pod weight (r = 0.906) at 1% level of significance. While, pod weight is negatively correlated with early leaf spot at 30 days (r = -0.401) and at 45 days (r = -0.410), late leaf spot at 70 days (r = -0.342) and at 85 days (r = -0.309) at 1% significant level. With the use of SSR markers, a total 16 alleles were recognized with a mean of 3.20 alleles per locus. The gene multiplicity differed from 0.6074 to 0.6491 for the markers Seq5D05 and PM137 respectively with an average of 0.6289. A total 18 alleles were produced by ISSR markers with an average of 3.6 alleles per locus. In our study, the gene diversity varied from 0.5877 to 0.6625 for the markers ISSR3 and ISSR21 respectively with an average of 0.6164. Diverse group of groundnut germplasm has been identified on the basis of SSR and ISSR markers along with morphological characterization.

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花生抗叶面真菌病害的表型和分子评价

花生改良任务通常集中在培育叶面真菌抗病品种上。花生早、晚叶斑病对花生种子的产量和品质造成重大损失。简单序列重复(SSR)标记具有较高的多态性扩增率，而简单序列间(ISSR)标记因其体积大、结果可重复性好而被广泛用于多样性评价。本研究利用96个花生基因型进行形态生理鉴定，田间条件下对早、晚叶斑病进行病害指标鉴定，并利用SSR和ISSR标记进行多样性评价。相关分析发现，籽粒重与收获指数、荚果重在1%的显著水平上呈极显著正相关(r = 0.906)。荚果重与30 d早斑(r = -0.401)、45 d晚斑(r = -0.410)、70 d晚斑(r = -0.342)、85 d晚斑(r = -0.309)呈1%显著负相关。利用SSR标记，共识别出16个等位基因，平均每个位点有3.20个等位基因。Seq5D05和PM137的基因多样性差异为0.6074 ~ 0.6491，平均为0.6289。ISSR标记共产生18个等位基因，平均每个位点产生3.6个等位基因。在我们的研究中，ISSR3和ISSR21的基因多样性范围分别为0.5877 ~ 0.6625，平均为0.6164。通过对花生种质资源的SSR和ISSR标记和形态鉴定，初步确定了花生种质资源的不同类群。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Crop Design

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