Cleaner Chemical Engineering最新文献

{"title":"Improved coloration of hemp fabrics via low-pressure argon plasma assisted surface modification","authors":"Kunal S. Bapat ,&nbsp;T.P. Kee ,&nbsp;S.J. Russell ,&nbsp;L. Lin","doi":"10.1016/j.clce.2024.100123","DOIUrl":"10.1016/j.clce.2024.100123","url":null,"abstract":"<div><p>Interest in hemp as a viable cellulosic fibre for clothing has increased, driven partly by its economic benefits and the importance of natural renewable materials in emerging circular economies. However, the coloration and chemical finishing of lignocellulosic fibres such as hemp typically require large quantities of water and chemicals. Argon plasma pretreatment provides a way of modulating the physical properties of hemp fibres to improve the coloration process without compromising other bulk properties such as tensile strength. Such plasma treatments may contribute to alleviating the negative environmental impacts associated with liquid pretreatments, heating, or the use of auxiliary chemicals. Dyeing of hemp fibres is particularly challenging due to its crystalline chemical structure. In this study, low-pressure argon plasma-assisted surface modification of woven hemp fabrics up to 600 s at 40 and 80 Hz was explored for enhanced dyeability, resulting in enhanced dye-fibre bonding. Fourier-transform infrared spectroscopy and Raman spectroscopy of argon plasma pretreated hemp fabrics produced no noticeable changes in the functional groups of the fibres, but a physiochemical modification was observed in terms of the density of polar groups. Scanning electron microscopy (SEM) images revealed marked morphological changes including nano-etching of the fibre surface at certain argon plasma process conditions. The pretreatment process increased fibre hydrophilicity, and enhanced reactivity of the surficial –OH groups towards fibre-reactive and vat dyes, resulting in higher colour strength in dyed woven hemp fabrics. Overall, we envisage such plasma pretreatments may impact positively on the material and energy efficiency of the hemp fabric dyeing process.</p></div>","PeriodicalId":100251,"journal":{"name":"Cleaner Chemical Engineering","volume":"10 ","pages":"Article 100123"},"PeriodicalIF":0.0,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772782324000081/pdfft?md5=404969b0c672df90975e0956492b9d6a&pid=1-s2.0-S2772782324000081-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyrolysis products of C2 hydrocarbons using thermal plasma","authors":"Yoshiya Matsukawa ,&nbsp;Aki Watanabe ,&nbsp;Yasuhiro Saito ,&nbsp;Yohsuke Matsushita ,&nbsp;Koki Era ,&nbsp;Takayuki Aoki ,&nbsp;Hideyuki Aoki","doi":"10.1016/j.clce.2024.100121","DOIUrl":"10.1016/j.clce.2024.100121","url":null,"abstract":"<div><p>Film-like carbon is expected to have various applications, and establishing a method for its mass production is highly desirable. Although there have been reports of obtaining film-like solid carbon using thermal plasma, knowledge about the growth behavior of this film-like carbon has not been sufficient. We analyzed the products and their growth behavior by pyrolysis experiments of C2 hydrocarbons with thermal plasma and investigated the pyrolysis behavior of C2 hydrocarbons with thermal plasma by numerical analysis. Solid products with different residence times were sampled from a sampling port in the reactor, observed by electron microscopy, and analyzed for crystallinity by Raman spectroscopy and X-ray diffraction. The solid products collected by a filter at the reactor outlet were measured by pyrolysis gas chromatography-mass spectrometer (GC/MS). The pyrolysis of acetylene yielded particulate carbon as in the pyrolysis in the electric furnace, whereas the pyrolysis of ethylene yielded a film-like carbon. The HRTEM image of ethylene pyrolysis products, however, shows lines indicating a stacked graphite structure of several tens of nanometers, indicating a different structure. In the pyrolysis GC/MS of ethylene pyrolysis products, various compounds were detected, whereas in the pyrolysis of acetylene, polycyclic aromatic hydrocarbons (PAHs) from three to seven rings were not detected. Reaction kinetic calculations using electron collision reactions were performed to examine the important reactions. The amount of ions produced tends to be larger for the pyrolysis of ethylene than for the pyrolysis of acetylene, indicating that the electron collision reaction is more likely to occur with ethylene in this calculation.</p></div>","PeriodicalId":100251,"journal":{"name":"Cleaner Chemical Engineering","volume":"10 ","pages":"Article 100121"},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772782324000068/pdfft?md5=4d8f68eefc1a6b5fcc8be6d3652505e2&pid=1-s2.0-S2772782324000068-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flexible and synergistic methanol production via biomass gasification and natural gas reforming","authors":"Mohammad Ostadi ,&nbsp;Leslie Bromberg ,&nbsp;Guiyan Zang ,&nbsp;Daniel R. Cohn ,&nbsp;Emre Gençer","doi":"10.1016/j.clce.2024.100120","DOIUrl":"10.1016/j.clce.2024.100120","url":null,"abstract":"<div><p>Sustainable liquid fuels are essential for decarbonization of various means of transportation which are challenging to address through electrification or hydrogen use. A possible method for producing low-carbon liquid fuel is through the thermochemical biomass to liquid (BTL) process. In this study, we conduct a technoeconomic-environmental analysis of two processes which take advantage of integration of natural gas reforming and biomass gasification, with the objective of improving the economics. By integrating H₂-rich syngas (a mixture of H₂/CO) obtained from natural gas reforming with carbon-rich syngas from biomass gasification, we harness synergistic effects. This combination allows us to achieve the optimal H₂/CO ratio required for methanol synthesis, while also ensuring efficient carbon utilization. In the first design, natural gas is reformed in an autothermal reformer (ATR) to produce syngas. A Solid Oxide Electrolysis Cell (SOEC) is utilized to supply the O₂ for both gasification and reforming processes. The H₂ produced by the SOEC adjusts the H₂ content in the syngas before the methanol synthesis reactor. In the second design, natural gas is reformed in a gas-heated-reformer (GHR) before an ATR, while an Air Separation Unit (ASU) produces the O₂ for the process. As a benchmark, the economics and flexible operation of both processes are compared to a conventional BTL process. In addition, the techno-economic impact of operating in biomass-only or natural gas-only modes are investigated. For a 134 MW_th plant with 50 % of entering carbon from biomass, the levelized cost of methanol (LCOMeOH) of ATR+SOEC case is 34 % higher than the BTL reference case, while that of ATR+GHR case is 24 % lower than the BTL reference case. A lifecycle analysis (LCA) is conducted for these designs. Utilizing renewable electricity and 50 % biogenic carbon, the ATR+SOEC case emits 908 kgCO_2e /tonne MeOH for a 100-year Global Warming Potential (GWP), while the ATR+GHR case emits 721 kgCO_2e /tonne MeOH. For a 20-year GWP, these emissions are 1055 and 915 kgCO_2e /tonne MeOH, respectively. These emissions correspond to more than 50 % reduction in LCA emissions when compared to natural gas based LCA emissions.</p></div>","PeriodicalId":100251,"journal":{"name":"Cleaner Chemical Engineering","volume":"10 ","pages":"Article 100120"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772782324000056/pdfft?md5=a66e9e4ebf5394b1fbf1420dce8c099a&pid=1-s2.0-S2772782324000056-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142168265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrodes from carbonized grass clippings for bioelectrochemical systems","authors":"Alexander Langsdorf ,&nbsp;Michael Halim ,&nbsp;Marianne Volkmar ,&nbsp;Markus Stöckl ,&nbsp;Ralf Harnisch ,&nbsp;Peter Hahn ,&nbsp;Roland Ulber ,&nbsp;Dirk Holtmann","doi":"10.1016/j.clce.2024.100118","DOIUrl":"https://doi.org/10.1016/j.clce.2024.100118","url":null,"abstract":"<div><p>One major obstacle to the commercialization of electrobiotechnological systems is the cost of materials, including expensive electrodes. Smart recycling as well as the use of renewable resources can contribute to producing electrodes more ecologically and economically. Green waste is a biogenic residual material that occurs mainly in urban areas and is currently not recycled to a sufficient extent. Here we show the fabrication of electrodes from carbonized grass clippings and their application in microbial electrosynthesis as well as microbial fuel cells. While the electrodes cannot compete with established metal competitors for water electrolysis in microbial electrosynthesis, they perform comparably to commercial graphite electrodes in microbial fuel cells. With <em>Geobacter sulfurreducens</em>, a current response can be recorded for more than six weeks. To the best of our knowledge, this is the first time that carbonized green waste has been used as an electrode material for bioelectrochemical systems. This demonstrates the potential of carbonized biological materials as a raw material for electrodes and presents a recycling alternative for green waste.</p></div>","PeriodicalId":100251,"journal":{"name":"Cleaner Chemical Engineering","volume":"9 ","pages":"Article 100118"},"PeriodicalIF":0.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772782324000032/pdfft?md5=380d7566b129e6efac23e7b9c95628de&pid=1-s2.0-S2772782324000032-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140951629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of cashew nut shell liquid as biofuel blended in diesel: Optimisation of blends using additive acetone–butanol–ethanol (ABE (361))","authors":"Sabba Gwoda ,&nbsp;Jérémy Valette ,&nbsp;Sayon Sadio dit Sidibé ,&nbsp;Bruno Piriou ,&nbsp;Joël Blin ,&nbsp;Igor W.K. Ouédraogo","doi":"10.1016/j.clce.2024.100117","DOIUrl":"https://doi.org/10.1016/j.clce.2024.100117","url":null,"abstract":"<div><p>In this study, the feasibility of increasing the proportion of cashew nut shell liquid (CNSL)-based biofuels in diesel was assessed. Biofuel–diesel blends with different percentages of CNSL were prepared, and their physical properties, including the density, viscosity, and heating value, were determined. B10 (CNSL/diesel = 10:90 v/v) satisfied the diesel specifications without preheating, whereas B20 (20:80 v/v) and B30 (30:70 v/v) met the specifications only after preheating to 60 and 80 °C, respectively. To avoid preheating B20 and B30, an acetone/butanol/ethanol (ABE) mixture (30:60:10 v/v) was added to the fuel blends to improve their flow characteristics. The blends with CNSL/ABE (361)/diesel ratios of 20:10:70 and 30:30:40 (v/v) exhibited properties comparable to those of diesel and remained stable for one month of storage. These fuel blends allow up to 30 vol% CNSL and 30 vol% ABE (361) to be incorporated into diesel and can be used as alternative fuels in diesel engines.</p></div>","PeriodicalId":100251,"journal":{"name":"Cleaner Chemical Engineering","volume":"9 ","pages":"Article 100117"},"PeriodicalIF":0.0,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772782324000020/pdfft?md5=831a7311f9a014eef271d23b378a5fd3&pid=1-s2.0-S2772782324000020-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140823936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrothermal depolymerization of spent biomass for production of lactic acid and small aromatics","authors":"Jyoti ,&nbsp;Nishant Pandey ,&nbsp;Pooja Negi ,&nbsp;Mangat Singh ,&nbsp;Bhuwan B. Mishra","doi":"10.1016/j.clce.2024.100116","DOIUrl":"https://doi.org/10.1016/j.clce.2024.100116","url":null,"abstract":"<div><p>Lactic acid synthesis from lignocelluloses can respond to the environmental concerns associated with producing this important commodity chemical for various applications, e.g., food, cosmetics, pharmaceuticals, etc. Pretreatment of palmarosa <em>(Cymbopogon martinii)</em> biomass with 1.0 % HNO₃ solution at 121 °C temperature and 1.03 bar pressure for 60 min under an autoclave conditions demonstrated a highly selective conversion of biomass contained hemicellulose to xylose in ∼17 wt % yield. A further hydrolytic depolymerization of pretreated biomass to liquid products was carried out <em>via</em> processing in the presence of anthraquinone (5 wt % loading) as a catalyst under temperature (250 °C) and pressure (15–16 bar, where, 1 bar = 10⁵ Pa) in dilute NaOH (1.5 M) solution for 60 min to afford a hydrolysate rich in carboxylic acids and small aromatics. Solvent extraction method was applied successfully to separate the aromatic compounds from the aqueous phase. The carboxylic acids were formed as the product of cellulose oxidation with good selectivity (&gt;45 %) towards the DL-lactic acid (∼14 wt % yield with respect to substrate biomass). Small aromatics, predominantly phenol was identified as the product of depolymerized lignin.</p></div>","PeriodicalId":100251,"journal":{"name":"Cleaner Chemical Engineering","volume":"9 ","pages":"Article 100116"},"PeriodicalIF":0.0,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772782324000019/pdfft?md5=1282811658a993f8b710a2a46761ab1b&pid=1-s2.0-S2772782324000019-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140142011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances, challenges, and prospects of electrochemical water-splitting technologies for net-zero transition","authors":"Hasan Ozcan ,&nbsp;Rami S. El-Emam ,&nbsp;Selahattin Celik ,&nbsp;Bahman Amini Horri","doi":"10.1016/j.clce.2023.100115","DOIUrl":"https://doi.org/10.1016/j.clce.2023.100115","url":null,"abstract":"<div><p>This review paper presents state-of-the-art electrolytic-based hydrogen production technologies capable of helping to achieve the “net-zero” targets. It covers the recent advances in electrochemical water-splitting technologies, considering their maturity, durability, and operational aspects related to their near-term deployment. This paper aims to critically assess electrochemical technologies compatible with renewables, nuclear, or other clean energy sources with a high potential to be applied for green hydrogen production at scale. It also discusses the techno-economic aspects as well as the technological readiness of the potential carbon-free hydrogen production routes based on electrochemical approaches. With a comprehensive survey of the recent literature and extensive insight into current developments, the issues associated with deploying the electrolytic water splitting technologies are discussed, along with a review of their market readiness level to play a potential role in the transition journey from fossil fuel-based-economy into hydrogen-driven energy infrastructure. In addition, the paper provides insight into the legal strategies and the governmental incentives required to reach the so-called “net-zero” emission plans globally to enable green hydrogen for deep decarbonization of the industrial, transportation, and residential sectors. Near-term projects to deploy large-scale clean hydrogen production announced globally are also listed with their expected contribution toward the zero-emission targets. The result of this review shows that wind and solar photovoltaic energies, as the two most preferred renewable sources, can be used to drive Proton Exchange Membrane (PEM) and Alkaline Electrolysers (AE) at a cost range of USD 2 - 3 /kg of H₂. This figure is expected to decrease by more than 40 % until 2030 if these electrolyzers can be deployed at a large scale.</p></div>","PeriodicalId":100251,"journal":{"name":"Cleaner Chemical Engineering","volume":"8 ","pages":"Article 100115"},"PeriodicalIF":0.0,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49723170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Yarrowia lipolytica potential for the biodegradation of poly(ethylene terephthalate) (PET) from mooring lines of Oil & Gas offshore platforms","authors":"Julio Cesar Soares Sales ,&nbsp;Alanna Medeiros Botelho ,&nbsp;Aparecida Selsiane Sousa Carvalho ,&nbsp;Luan Giudicelli ,&nbsp;Aline Machado de Castro ,&nbsp;Bernardo Dias Ribeiro ,&nbsp;Priscilla Filomena Fonseca Amaral ,&nbsp;Maria Alice Zarur Coelho","doi":"10.1016/j.clce.2023.100109","DOIUrl":"https://doi.org/10.1016/j.clce.2023.100109","url":null,"abstract":"<div><p>Poly(ethylene terephthalate) (PET) is a widely used polyester with several applications, including offshore platforms and mooring lines of the Oil &amp; Gas industry. Due to the widespread use of PET and the growing concern about its environmental impact, biotechnology emerges as a potential solution to mitigate this problem. This work aims to evaluate, for the first time, the potential of <em>Y. lipolytica</em> IMUFRJ 50682 in the biodegradation of PET from waste mooring lines of Oil &amp; Gas offshore platforms. In this study, the biodegradation of PET from mooring lines by the yeast <em>Yarrowia lipolytica</em> was investigated through submerged cultivation or solid-state fermentation. After fermentation was complete, the biodegradation of PET sampled from the culture medium was evidenced by an increase in transmittance in areas related to the ester group detected through Attenuated Total Reflectance Fourier-Transform Infrared Spectroscopy (ATR-FTIR) for both culture strategies and all media tested. Besides, the crystallinity of PET increased by 79% and 72% after submerged cultivation using CSL-G + DMSO medium (2% corn steep liquor, 2% glycerol, and 5% Dimethyl sulfoxide) and after solid-state fermentation, respectively. Scanning electron microscopy revealed the formation of scratches and erosions on the polymer's surface after the microbial cultivation. The presence of PET monomer, terephthalic acid (TPA) (up to 142 µmol L ⁻ ¹), and some intermediates, such as bis(2-hydroxyethyl) terephthalate (BHET) and mono-(hydroxyethyl) terephthalate (MHET) in the medium after <em>Y. lipolytica</em> growth in the presence of PET proves that the polymer is being degraded. These results suggest a good biotechnological potential of <em>Y. lipolytica</em> for the depolymerization of PET from mooring lines.</p></div>","PeriodicalId":100251,"journal":{"name":"Cleaner Chemical Engineering","volume":"7 ","pages":"Article 100109"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49706272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the corrosion inhibition performance of snail slime on the exposed surface of mild steel in acidic environment","authors":"Ifeanyi Emmanuel Chukwunyere ,&nbsp;Titus Chinedu Egbosiuba","doi":"10.1016/j.clce.2023.100114","DOIUrl":"https://doi.org/10.1016/j.clce.2023.100114","url":null,"abstract":"<div><p>Corrosion of metals is a major issue in various industries and the use of organic materials, especially plant extracts, as alternatives to toxic chemicals in corrosion inhibitors is gaining attention. However, the potential of animal products as corrosion inhibitors has not been fully explored. This study investigates the corrosion inhibiting potential of snail slime (SS) for mild steel in 0.5 M H₂SO₄ solution using Gravimetric and electrochemical methods, including open circuit potential (OCP), potentiodynamic polarization (PDP), and electrochemical impedance spectroscopy (EIS). Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) were used for material characterization. The results indicate that snail slime has a high inhibition efficiency of up to 92.75%, with inhibition efficiency decreasing as temperature and immersion time increase but it increased with higher inhibitor concentrations. Adsorption studies showed that snail slime exhibited strong adherence to the Langmuir and Temkin adsorption isotherms, indicating spontaneous adsorption on mild steel. The inhibition mechanism shows typical physical adsorption and follows first-order kinetics. While the EIS analysis indicated a charge transfer-controlled corrosion process with the highest inhibition efficiency of 81.74%, the PDP analysis revealed that snail slime acted as a mixed-type inhibitor with the highest inhibition efficiency of 81.98%. FTIR reveals the functional groups responsible for the inhibition exhibited by snail slime inhibitors, including C<img>H, O<img>H, C<img>O-O, C<img>Cl, and N<img>H. SEM shows that the inhibition of corrosion is due to the formation of an insoluble stable protective film on the sample surface by an adsorption process. These findings suggest that snail slime has significant potential as an eco-friendly alternative to synthetic corrosion inhibitors in industrial applications.</p></div>","PeriodicalId":100251,"journal":{"name":"Cleaner Chemical Engineering","volume":"7 ","pages":"Article 100114"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49706284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a novel, efficient and fast method for colorimetric detection of multiple Salmonella serovars based on aptamer-nanoparticle biosensor","authors":"Verônica Wisineski Voltolini Neta ,&nbsp;Alexsandra Valério ,&nbsp;Karina Cesca ,&nbsp;Vendelino Oenning Neto ,&nbsp;Débora de Oliveira ,&nbsp;Dachamir Hotza","doi":"10.1016/j.clce.2023.100113","DOIUrl":"https://doi.org/10.1016/j.clce.2023.100113","url":null,"abstract":"<div><p>In this research, the stability, specificity, and sensibility of a colorimetric detection method based on gold nanoparticles conjugated with aptamers for detection of <em>Salmonella enterica</em> serovar <em>Typhimurium</em> was investigated. The synthesis of gold nanoparticles by citrate reduction and conjugation with aptamers were studied to obtain high stability over time and to hinder inaccurate results. The reaction conducted at initial pH 5.0 and molar ratio 3.5:1 Na3Ct to HAuACl₄ produced stable, monodisperse and spherical particles, with a mean particle diameter of 18 nm. Gold nanoparticles showed stability upon salt exposure limited to 120 mmol/L, which was enhanced with aptamer conjugation to 700 mmol/L. Experiments with different microorganisms confirmed the biosensor specificity to <em>Salmonella</em>, with a detection limit of 10³ CFU/mL for <em>S. Typhimurium,</em> as well as 10⁴ CFU/mL for S. <em>minnesota</em>, S. <em>heidelberg</em>, and <em>Salmonella</em> spp. The assays carried out with artificially contaminated samples and environmental samples resulted in a detection time and detection limit identical to those obtained with pure samples. Results confirm that the biosensor synthesized is a great alternative for detection methods, making it possible to improve time and efficiency detection in the routine inspections as well as reduce costs.</p></div>","PeriodicalId":100251,"journal":{"name":"Cleaner Chemical Engineering","volume":"7 ","pages":"Article 100113"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49760742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}