{"title":"Investigations on production of cow urine distillate using solar distillation system for cow shelter livelihoods","authors":"Vikrant P. Katekar","doi":"10.1016/j.clcb.2025.100143","DOIUrl":"10.1016/j.clcb.2025.100143","url":null,"abstract":"<div><div>Finance is the most pressing problem for cow shed managers and cow owners. Despite government intentions and donor money, cow owners and cow shed operators confront several challenges while managing cows in the shelters. Cows in shelters produce less milk but more urine, which may be used to generate income. Farmers and cow shelter operators may distil cow urine and sell it for medication and fertiliser. Considering this, the authors designed and tested a cost-effective, novel solar thermal hydrodistillation system for cow urine distillation. In addition, the effectiveness of extracted cow urine distillate was also studied. The distillate's most significant ingredient was phosphonic acid (81.1 %). It is critical antiviral medication cures cancer, HIV, and hepatitis B in prodrug formulations. Solar-powered cow urine distillate is odourless and drinkable without dilution. This distillate had 0.88 % ammonia and 2.25 % nitrogen, which was 80 % less than the market-based cow urine distillate. This investigation also showed that cow-urine distillate is anxiolytic and anti-depressant. A dosage of 40 % cow urine distillate is helpful in diabetes management. Cancer cells are reduced when cow urine distillate dose exceeds 10 %. It is clear that the solar thermal hydrodistillation system for cow urine distillation is helpful to cow owners and cow shed managers. Prepared cow urine distillate is useful for treating many life-threatening diseases.</div></div>","PeriodicalId":100250,"journal":{"name":"Cleaner and Circular Bioeconomy","volume":"10 ","pages":"Article 100143"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Optimization of production of detergent-compatible and thrombolytic protease by Aspergillus flavus AT grown on agrowastes using Taguchi technique cum nanoparticles supplementation","authors":"V.A. Ajayi, A. Lateef","doi":"10.1016/j.clcb.2025.100144","DOIUrl":"10.1016/j.clcb.2025.100144","url":null,"abstract":"<div><div>Microbial proteases are important industrial enzymes with diverse applications. However, their production is expensive due to the cost of media, thereby necessitating the search for cheap substrates for their production. In this study, novel optimization regimen was applied to increase protease production by a local strain of <em>Aspergillus flavus</em> by deploying the Taguchi technique with the incorporation of ZnONPs. The isolate produced protease in both SmF and SSF by utilizing groundnut peel (GP), groundnut shell (GS) and melon seed shell (MS) with titers in the range of 24.20–45.65 U/ml for SmF and 18.15–152.90 U/g for SSF, respectively. Its protease production was further optimized in the underutilized MS, which has not been reported for protease production until now. Taguchi cum nanosupplemention in SmF increased protease production from 37.40–185.25 U/ml as the global optimum with the inclusion of ZnONPs accounting for up to 10.74 % of the production. The improved production of protease was 4.95 fold compared with the unoptimized bioprocess. The protease was effective for blood stain removing and showed enhanced proteolytic activities of 254.72–329.10 U/ml corresponding to performance of 137.50–177.65 % in the presence of four detergents indicating high level of compatibility. The protease also dissolved blood clot by 91.30 %, thereby making it useful as thrombolytic agent. For the first time, we report the microbial production of protease using MS-based media and enhanced its production via incorporation of nanoparticles. Thus, the detergent-compatible protease produced in this study has industrial and biomedical applications.</div></div>","PeriodicalId":100250,"journal":{"name":"Cleaner and Circular Bioeconomy","volume":"10 ","pages":"Article 100144"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exploring the role of nature-based solutions and emerging technologies in advancing circular and sustainable agriculture: An opinionated review for environmental resilience","authors":"Eliakira Kisetu Nassary","doi":"10.1016/j.clcb.2025.100142","DOIUrl":"10.1016/j.clcb.2025.100142","url":null,"abstract":"<div><div>This review explores the role of Nature-based Solutions (NbS) and emerging technologies in advancing circular and sustainable agriculture, with a focus on enhancing environmental resilience. NbS, such as agroforestry and cover cropping, integrate ecological processes to improve sustainability, biodiversity conservation, and climate resilience. These practices enhance soil health, increase organic matter, improve soil structure, and promote nutrient availability, thereby mitigating erosion and fostering biodiversity. Additionally, they support critical ecosystem services like pollination and pest control, which are essential for climate change adaptation. However, the scalability of NbS is hindered by challenges, including high initial costs, knowledge gaps, and limited market access for organic products. While NbS can help mitigate environmental degradation, intensified agricultural practices in some cases may still lead to concerns like habitat loss and water pollution from agricultural runoff. Emerging technologies such as precision agriculture, genetic innovations, and drone technologies offer promising solutions to optimize resource use, enhance crop resilience, and facilitate real-time monitoring, supporting sustainable agricultural practices. The review calls for further research and policy efforts to address adoption barriers, overcome knowledge gaps, and mitigate environmental risks. Circular and sustainable agriculture can be advanced, offering significant benefits for environmental resilience and food security by integrating NbS and emerging technologies.</div></div>","PeriodicalId":100250,"journal":{"name":"Cleaner and Circular Bioeconomy","volume":"10 ","pages":"Article 100142"},"PeriodicalIF":0.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daphine Kamusingize , Esther Ronner , Godfrey Taulya , Rosa de Vos , Priver Namanya , Jerome Kubiriba , Katrien Descheemaeker
{"title":"Operationalising the East African bioeconomy strategy: An assessment of industrial readiness of Uganda's banana fibre sector","authors":"Daphine Kamusingize , Esther Ronner , Godfrey Taulya , Rosa de Vos , Priver Namanya , Jerome Kubiriba , Katrien Descheemaeker","doi":"10.1016/j.clcb.2025.100141","DOIUrl":"10.1016/j.clcb.2025.100141","url":null,"abstract":"<div><div>With SDG targets due in 2030, transitioning from a fossil-based to a green economy is urgent. Key to this transition is increased use of agricultural residues, e.g., banana pseudo-stems, as raw material for bio-based industries. However, a knowledge gap exists on the current level of Industrial Readiness (<em>IR</em>) of various bio-based value chains, beyond utilization options for such materials. We conducted a multi-dimensional sustainability assessment of the status of <em>IR</em> of Uganda's banana fibre sector and identified strategic requirements to sustainably upscale production and processing. Systematically, we collected and analysed data obtained through resource mapping, interviews and FGDs to: (i) map biomass flows; (ii) map relations among actors; (iii) assess readiness of farmers to supply raw material; and (iv) describe, from a stakeholders’ perspective, the factors relevant in advancing the industry towards readiness. Results indicate that banana fibre biomass flows followed a complex pathway from pseudo-stem production to marketing and waste disposal, in which fibre processors were the most influential actors. Out of 102 farmers assessed, 40 % were ready (with <em>ability</em>) to sell pseudo-stems. Yet, over 70 % expressed interest to sell on condition of substantial price-increase. To achieve <em>IR,</em> various aspects were at nascent status of readiness but require attention: markets; actor partnerships; public awareness; human, technical and infrastructural capacity; and sustainability issues. Ultimately, sectoral transformation to industrial level is timely. However, it requires joint action to expand markets, targeted investment including policy implementation, and negotiation of trade-offs among actors. Also, enhancing methods developed for future evaluation of similar industries.</div></div>","PeriodicalId":100250,"journal":{"name":"Cleaner and Circular Bioeconomy","volume":"10 ","pages":"Article 100141"},"PeriodicalIF":0.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Advanced nano-enhanced bioplastics for smart food packaging: Enhancing functionalities and sustainability","authors":"Dilip Kumar Chandra , Awanish Kumar, Chinmaya Mahapatra","doi":"10.1016/j.clcb.2025.100140","DOIUrl":"10.1016/j.clcb.2025.100140","url":null,"abstract":"<div><div>Plastics have dominated food packaging due to their cost-effectiveness, lightweight characteristics, and durability. However, their permeability to gases and moisture has adversely affected food quality and worsened climate change through emissions and waste. Bioplastics have emerged as sustainable alternatives, offering biodegradability and biobased origins, but have initially grappled with higher production costs and suboptimal mechanical and barrier properties compared to conventional plastics. This review evaluates the limitations of biopolymer-based bioplastics, including inadequate mechanical strength and high hydrophilicity leading to water absorption, have necessitated functional additives. And explores recent innovations reveal that embedding nanoparticle—such as zinc oxide (ZnO), silver nanoparticles (AgNPs), titanium dioxide (TiO₂), copper nanoparticles (CuNPs), reduced graphene oxide (RGO), and nanoclays—into bioplastics has yielded materials with improved tensile strength, Young's modulus, thermal stability, and reduced water vapor permeability. The integration of nanomaterials into bioplastics has augmented the physicomechanical properties and conferred advanced antimicrobial efficacy, UV photoprotection, and tunable biodegradability. For instance, ZnO and TiO₂ nanoparticles have significantly improved mechanical robustness and barrier performance, while RGO and CuNPs have exhibited potent antimicrobial activity. These nano-enhanced bioplastics has addressed deficiencies in conventional bioplastics and expanded their use in food packaging and biomedicine. This review highlights progress in biopolymer-based bioplastics through nanoparticle incorporation, emphasizing their enhanced attributes and promoting sustainable solutions.</div></div>","PeriodicalId":100250,"journal":{"name":"Cleaner and Circular Bioeconomy","volume":"10 ","pages":"Article 100140"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143311060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ramanujam Srinivasan Vethathirri , Ezequiel Santillan , Yissue Woo , Sara Swa Thi , Hui Yi Hoon , Stefan Wuertz
{"title":"Towards sustainable aquafeeds: Safe and consistent microbial protein grown on food-processing wastewater","authors":"Ramanujam Srinivasan Vethathirri , Ezequiel Santillan , Yissue Woo , Sara Swa Thi , Hui Yi Hoon , Stefan Wuertz","doi":"10.1016/j.clcb.2025.100139","DOIUrl":"10.1016/j.clcb.2025.100139","url":null,"abstract":"<div><div>Microbial community-based single cell protein (SCP) holds promise as a sustainable source of protein in livestock feed; yet its feed-safety and consistency in composition and production when using variable real-world wastewater has not been investigated. Here, the effect of heterogeneity in soybean-processing wastewater on SCP quality was tested using four replicate sequencing batch reactors over 92 days. The microbial community-based SCP grown on soybean wastewater demonstrated high consistency, with replicates showing similar patterns of biomass growth and protein accumulation. The dry microbial biomass exhibited a protein content of 39.8 ± 5.8 %, and the yield was 17.7 ± 1.7 g dry weight/g soluble total Kjeldahl nitrogen (sTKN). <em>Azospirillum</em>, a nitrogen-fixing bacterium, was the prevalent SCP-producing genus in all replicates at a relative abundance of 40.6 ± 5.1 %. The organism was not detected in wastewater, where <em>Lactococcus</em> and <em>Weissella</em> dominated. SCP contained essential amino acids to supplement conventional animal diets and was deemed safe for fish due to the very low abundance of fish-pathogen-like sequences (< 0.009 %) via metabarcoding. This study demonstrates the consistency of microbial community-based SCP derived from food-processing wastewater and addresses feed safety through pathogen screening, highlighting its potential to substitute protein in traditional animal feed and contribute to sustainable aquaculture practices.</div></div>","PeriodicalId":100250,"journal":{"name":"Cleaner and Circular Bioeconomy","volume":"10 ","pages":"Article 100139"},"PeriodicalIF":0.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The potential of agri-food waste to solve construction's environmental problems: A review","authors":"Margarida Soares , Luciana Faria , Tiago Miranda , Eduardo Pereira , Cândida Vilarinho , Joana Carvalho","doi":"10.1016/j.clcb.2025.100138","DOIUrl":"10.1016/j.clcb.2025.100138","url":null,"abstract":"<div><div>The transition from a linear to a circular economy became essential for the dissociation between economic development and increasing resource consumption. The management of agri-food waste requires special attention, as a large part is deposited in landfills or incinerated. It is essential to explore innovative solutions that allow the application of agri-food waste in different sectors. Official data stated that every year, around 500 million tons of waste are generated globally in the agricultural sector (Yaashikaa, Senthil Kumar, and Varjani 2022). Conversely, the construction industry is resource intensive and therefore causes serious and diverse environmental impacts, from the depletion of natural resources to the generation of great amounts of waste. It is therefore urgent to search for solutions to reduce these impacts through the implementation of the circular economy. Several studies have demonstrated the potential of using agri-food residues as products for building materials, some of which improve their durability and their physical and mechanical properties. The use of these wastes/by-products enables the construction of more sustainable buildings, contributing to the reduction of the environmental impact associated with both agri-food and construction sectors. This work aims to develop a systematic literature review to identify, evaluate, and integrate the current success cases and applications, as well as relevant evidence on this subject, to identify promising approaches and trends for future agri-food /construction integrated development toward sustainability and circularity. To achieve this, 50 research papers in this context, published between 2020 and 2023, were analyzed and discussed.</div></div>","PeriodicalId":100250,"journal":{"name":"Cleaner and Circular Bioeconomy","volume":"10 ","pages":"Article 100138"},"PeriodicalIF":0.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143094558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biodegradability of tomato stem-reinforced composites: Towards a virtuous approach to local and circular waste upcycling","authors":"Estelle Bonnin , Méline Calatraba , Xavier Gabrion , Camille Alvarado , Coralie Buffet , Arnaud Day , Lèna Brionne , Alain Bourmaud , Johnny Beaugrand","doi":"10.1016/j.clcb.2025.100136","DOIUrl":"10.1016/j.clcb.2025.100136","url":null,"abstract":"<div><div>The current method of producing tomatoes in greenhouses uses petro-sourced plastic accessories that contaminate plant waste when the greenhouses are emptied. For this reason, this study aims to develop a biodegradable material to replace plastic accessories. To evaluate the feasibility of using tomato byproduct as reinforcements in a range of biobased and biodegradable thermoplastic materials, the compound degradability was investigated though biochemical and imaging approaches. The first set of experiments carried out on the tomato stem showed that the enzymatic degradation by a mixture of cellulases and pectinases efficiently removed constitutive biopolymers, and that the average size and the polydispersity decreased during treatment. The largest particles became more irregular, highlighting the enzyme-recalcitrant domains. When compounded with different matrix polymers (PBS, PBAT/PHA or PBAT/PLA), tomato stem particles remained susceptible to enzymatic degradation. Tomography analysis showed that all the degraded samples exhibited a large increase in porosity, the largest increase being observed in the PLA-containing specimens.</div><div>This fully circular approach from waste to useful compounds for horticulture and market gardening is a promising way of upcycling tomato biomass, compatible with end-of-life composting.</div></div>","PeriodicalId":100250,"journal":{"name":"Cleaner and Circular Bioeconomy","volume":"10 ","pages":"Article 100136"},"PeriodicalIF":0.0,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143094555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Babajide S. Kosemani , Ayoola A. Babalola , Titus A. Ilori
{"title":"Development and optimization of small- scale inverter-powered incubator for egg hatchability system","authors":"Babajide S. Kosemani , Ayoola A. Babalola , Titus A. Ilori","doi":"10.1016/j.clcb.2025.100137","DOIUrl":"10.1016/j.clcb.2025.100137","url":null,"abstract":"<div><div>An indigenous small-scale inverter powered incubator for egg hatchability system was developed. The aim of this study was to produce egg hatchability system using locally available materials for small and medium scale farmers. The major components of the systems include incubation box, heating system, heat circulation system and egg turning mechanism. The Box-Behnken design (BBD) of Response surface methodology was used to study the influence of input variables and optimize the incubation conditions. Temperature (36, 37.5, and 39 °C), humidity (50, 55, and 60%), and air flow (0.2, 0.3, and 0.4 m/s) were input variables and hatchability was the output variable. Regression model for the hatchability system was developed, and the optimum incubation condition was determined and tested to validate the model. The results showed that hatchability of the incubator ranges from 55 to 98.4%. The developed regression model adequately described the hatchability. The input variables significantly influenced the performance responses. The optimum incubating temperature, humidity, and airflow were 37.08°C, 57.57%, and 0.25 m/s, respectively. The predicted optimum hatchability obtained under these incubating conditions was 99.15%. The experimental (test) hatchability values obtained at the optimal incubation conditions during validation was 98.70%. The test value being relatively close to the predicted value of responses, with percentage error values less than 10%, indicates that the difference between the experimental (test) data and the predicted data is within the acceptable range, confirming the suitability of the optimal incubating conditions produced by the RSM. These findings suggested that the developed automatic electric incubator can be used to produce chicks commercially.</div></div>","PeriodicalId":100250,"journal":{"name":"Cleaner and Circular Bioeconomy","volume":"10 ","pages":"Article 100137"},"PeriodicalIF":0.0,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143094554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gabriela Solidario de Souza Benatti , Antônio Márcio Buainain , Pedro Gilberto Cavalcante Filho , Ricardo Vargas-Carpintero , Lotte Asveld , Patricia Osseweijer
{"title":"Macaw palm (Acrocomia spp.): An opportunity for including smallholders in Brazil's biodiesel production","authors":"Gabriela Solidario de Souza Benatti , Antônio Márcio Buainain , Pedro Gilberto Cavalcante Filho , Ricardo Vargas-Carpintero , Lotte Asveld , Patricia Osseweijer","doi":"10.1016/j.clcb.2025.100134","DOIUrl":"10.1016/j.clcb.2025.100134","url":null,"abstract":"<div><div>Promoting inclusive development through public policies is a complex task that presents different challenges and even controversies. The National Program for Biodiesel Production and Use (PNPB) in Brazil is one example of an effort to promote sustainable development within an inclusive strategy with good intentions but many challenges. One of the PNPB goals is to diversify feedstocks for biodiesel production, and oils from the <em>Acrocomia spp.</em> palm genus have the potential to meet this goal. As acrocomia's value chain is under development in Brazil, particularly with a focus on the macaúba palm (<em>Acrocomia aculeata</em>), and in different regions of Latin America, we state that it is possible to develop it with the inclusion of smallholder farmers. In this sense, this paper focuses on analyzing the challenges and opportunities for smallholder productive inclusion in the value chain. Our main question is: How can the participation of smallholders in the cultivation of macaw palm be promoted? The answer to this question is based on literature and field research carried out by the authors. Our study finds opportunities for inclusion, but also four main challenges to be considered with caution: i) the shift from agroextractivism to commercial plantations, ii) the limited acreage available to some small farmers, iii) the slow pace in generating economic results, and iv) negative past experiences with other crops. We emphasize the importance of development strategies that offer incentives, mitigate risks, and guarantee greater security in decision-making for those involved. The available literature about the acrocomia value chain focuses mainly on technical and agronomic aspects, with few outputs on inclusion. In this sense, this paper calls attention to the development of the novel biobased value chains from the acrocomia palm without leaving social responsibility behind.</div></div>","PeriodicalId":100250,"journal":{"name":"Cleaner and Circular Bioeconomy","volume":"10 ","pages":"Article 100134"},"PeriodicalIF":0.0,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143094556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}