Chem & Bio Engineering最新文献

{"title":"An MOF-Based Single-Molecule Propylene Nanotrap for Benchmark Propylene Capture from Ethylene.","authors":"Jia-Xin Wang, Teng-Fei Zhang, Jiyan Pei, Di Liu, Yu-Bo Wang, Xiao-Wen Gu, Guodong Qian, Bin Li","doi":"10.1021/cbe.4c00102","DOIUrl":"10.1021/cbe.4c00102","url":null,"abstract":"<p><p>Highly selective capture and separation of propylene (C₃H₆) from ethylene (C₂H₄) presents one of the most crucial processes to obtain pure C₂H₄ in the petrochemical industry. The separation performance of current physisorbents is commonly limited by insufficient C₃H₆ binding affinity, resulting in poor low-pressure C₃H₆ uptakes or inadequate C₃H₆/C₂H₄ selectivities. Herein, we realize a unique single-molecule C₃H₆ nanotrap in an ultramicroporous MOF material (Co(pyz)[Pd(CN)₄], ZJU-74a-Pd), exhibiting the benchmark C₃H₆ capture capacity at low-pressure regions. This MOF-based nanotrap features the sandwichlike strong multipoint binding sites and the perfect size match with C₃H₆ molecules, providing an ultrastrong C₃H₆ binding affinity with the maximal <i>Q</i> _st value (55.8 kJ mol^-1). This affords the nanotrap to exhibit one of the highest C₃H₆ uptakes at low pressures (60.5 and 103.8 cm³ cm^-3 at 0.01 and 0.1 bar) and record-high C₃H₆/C₂H₄ selectivity (23.4). Theoretical calculations reveal that the perfectly size-matched pore cavities combined with sandwichlike multibinding sites enable this single-molecule C₃H₆ nanotrap to maximize the C₃H₆ binding affinity, mainly accounting for its record low-pressure C₃H₆ capture capacity and selectivity. Breakthrough experiments further confirm its excellent separation capacity for actual 1/99 and 50/50 C₃H₆/C₂H₄ mixtures, affording the remarkably high pure C₂H₄ productivities of 17.1 and 3.4 mol kg^-1, respectively.</p>","PeriodicalId":100230,"journal":{"name":"Chem & Bio Engineering","volume":"1 11","pages":"952-959"},"PeriodicalIF":0.0,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11835284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143461489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NH₂-MIL-125 Nanosheets Prepared via Crystallization Kinetics Modulation for Ultrathin Membrane Fabrication.","authors":"Yanwei Sun, Jiahui Yan, Mingming Wu, Jie Jiang, Yi Liu","doi":"10.1021/cbe.4c00103","DOIUrl":"10.1021/cbe.4c00103","url":null,"abstract":"<p><p>Regulating both crystallographic orientation and thickness of titanium metal-organic framework (Ti-MOF) membranes remains a significant challenge. In this study, we pioneered the fabrication of uniform 29 nm thick NH₂-MIL-125 nanosheet seeds by employing crystallization kinetics modulation approach. Through innovating confined counter-diffusion-assisted epitaxial growth under single-mode microwave heating, a highly <i>c</i>-oriented 80 nm thick NH₂-MIL-125 membrane was prepared. Significant reduction in thickness endowed the membrane with unprecedented H₂ permeance (1350 GPU) along with considerable H₂/CO₂ selectivity (19.1), exceeding the performance benchmarks of state-of-the-art NH₂-MIL-125 membranes.</p>","PeriodicalId":100230,"journal":{"name":"Chem & Bio Engineering","volume":"1 10","pages":"855-862"},"PeriodicalIF":0.0,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11835268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143461453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR-Mediated rDNA Integration and Fluorescence Screening for Pathway Optimization in Pichia pastoris","authors":"Xiaojing Jiang,&nbsp;Mengxin Li,&nbsp;Zhijiao Wang,&nbsp;Cuifang Ye,&nbsp;Jucan Gao,&nbsp;Xiaowei Ai,&nbsp;Jingfei Bao,&nbsp;Jintao Cheng and Jiazhang Lian*,&nbsp;","doi":"10.1021/cbe.4c0010410.1021/cbe.4c00104","DOIUrl":"https://doi.org/10.1021/cbe.4c00104https://doi.org/10.1021/cbe.4c00104","url":null,"abstract":"<p >Gene dosage amplification is an effective strategy to improve the performance of heterologous genes and pathways. <i>Pichia pastoris</i> is an excellent recombinant protein expression host with high efficiency in protein folding and glycosylation. However, the traditional iterative multicopy integration method typically faces challenges such as being time-consuming and having high cost and potential gene mutations. Accordingly, we established <u>C</u>RISPR-mediated <u>r</u>DNA <u>i</u>ntegration and fluorescence <u>s</u>creening for <u>p</u>athway <u>o</u>ptimization (CRISPO) for multicopy pathway integration in a single-step and antibiotic-free manner. With geraniol biosynthesis as a case study, we designed CRISPO based on the use of glycerol-induced and glucose-repressed promoters (CRISPOi) or strong constitutive promoters (CRISPOc) to drive the expression of the red fluorescent protein mCherry as the screening marker. We employed CRISPOi for stable strain construction by multicopy integration of the geraniol synthase encoding gene, achieving a 19.5-fold increase in geraniol production. We demonstrated CRISPOc for visualizing and determining the rate-limiting steps of the mevalonate pathway, with HMG1 and ERG12 identified as the major rate-limiting enzymes through two rounds of exploration. Ultimately, CRISPO enabled us to construct an engineered <i>P. pastoris</i> strain producing 1.66 g/L geraniol (with a total of 2.12 g/L monoterpenoids) and 6.27 g/L geraniol (with a total of 6.48 g/L monoterpenoids) in 24-well plates and 5 L fermenters, respectively, representing the highest titer and productivity of geraniol ever reported. CRISPO is an important addition to the synthetic biology toolbox for the construction and optimization of <i>P. pastoris</i> cell factories.</p>","PeriodicalId":100230,"journal":{"name":"Chem & Bio Engineering","volume":"1 11","pages":"940–951 940–951"},"PeriodicalIF":0.0,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbe.4c00104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143127503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Side Chain Crosslinked Anion Exchange Membrane for Acid Concentration by Electrodialysis","authors":"Haoyang He,&nbsp;Qian Chen,&nbsp;Rongqiang Fu,&nbsp;Zhaoming Liu,&nbsp;Liang Ge* and Tongwen Xu*,&nbsp;","doi":"10.1021/cbe.4c0009610.1021/cbe.4c00096","DOIUrl":"https://doi.org/10.1021/cbe.4c00096https://doi.org/10.1021/cbe.4c00096","url":null,"abstract":"<p >Electrodialysis (ED) technology for waste acid treatment has high economic efficiency and environmentally friendly advantages. The primary limitation of ED in the retrieval of low-concentration spent acids lies in the leakage of hydrogen ions through anion exchange membranes (AEMs) due to its extremely small size and high mobility. To address this issue, a series of AEMs named QPAB-<i>x</i> (<i>x</i> = 3, 5, 7, 10) were designed for acid concentration in ED process by increasing the membrane densities through in situ crosslinking in this study. The successful synthesis of polymers was confirmed through ¹H nuclear magnetic resonance hydrogen (¹H NMR) spectroscopy and Attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy. Furthermore, ATR-FTIR spectroscopy showed that the higher the side chain content, the higher the crosslinking degree of the membranes. X-ray photoelectron spectroscopy (XPS) was employed to characterize the effects of aqueous and acidic environments on QPAB membranes. The performance disparities between QPAB-<i>x</i> membranes in acidic and aqueous environments were examined separately. Subsequently, the influence of crosslinking degree on the acid-blocking capability of the membranes was thoroughly investigated by conducting ED acid-concentration experiments to monitor the hydrogen ions concentration process and determine the current efficiency and energy consumption of the QPAB-<i>x</i> membranes. Our experimental results demonstrated that QPAB-<i>x</i> membranes with higher cross-linking degrees have lower water content, especially the QPAB-10 membrane with an IEC of approximately 1.5 mmol g^–1 and a remarkably low water content of around 10%. This leads to a reduced H⁺ transfer number and excellent acid-blocking properties. Additionally, compared to commercial membrane A2, using the QPAB-10 membrane in the ED process resulted in a higher final H⁺ concentration in the concentrated chamber. Consequently, these synthesized membranes exhibit considerable promise in the field of ED acid recovery.</p>","PeriodicalId":100230,"journal":{"name":"Chem & Bio Engineering","volume":"1 7","pages":"647–657 647–657"},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbe.4c00096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142020321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycopolypeptide Coordinated Nanovaccine: Fabrication, Characterization, and Antitumor Immune Response","authors":"Yingying Song,&nbsp;Lin Teng,&nbsp;Yanzheng Chen and Chang-Ming Dong*,&nbsp;","doi":"10.1021/cbe.4c0008310.1021/cbe.4c00083","DOIUrl":"https://doi.org/10.1021/cbe.4c00083https://doi.org/10.1021/cbe.4c00083","url":null,"abstract":"<p >Cancer nanovaccine is a frontier immunotherapy strategy, in which the delivery carrier can protect antigen and adjuvant from degradation, increase blood circulation half-life, and improve antigen permeability and presentation, thus enhancing the security and potency of nanovaccine. To address the barriers of antigen delivery, we design and fabricate a kind of intracellular pH-sensitive glycopolypeptide coordinated nanovaccine (OVA-HPGM-Mn) with ∼30% loading capacity of ovalbumin (OVA). The nanovaccine OVA-HPGM-Mn could specifically deliver antigen to dendritic cells (DCs) and effectively escape from endolysosomes to cytoplasm after 6 h of incubation, while the blank counterpart HPGM-Mn acted as an adjuvant to promote DCs maturation and increase the percentage of maturated cells to 26.5% from 11.8% in vitro. Furthermore, the mannosylated polypeptide nanovaccine prolonged the retention time of OVA for 72 h to facilitate 29.5% DCs maturation in lymph nodes, activated 48.8% CD8⁺T cells in spleen, increased the CD8⁺/CD4⁺T cell ratio twice to 1.06, and upregulated the levels of pro-inflammatory cytokines including TNF-α, IFN-γ, and IL-6, thus inhibiting the tumor growth of ∼80%. Consequently, this work provides a versatile strategy for the fabrication of glycosylated polypeptide coordinated nanomaterials for antigen delivery and cancer immunotherapy.</p>","PeriodicalId":100230,"journal":{"name":"Chem & Bio Engineering","volume":"1 7","pages":"633–646 633–646"},"PeriodicalIF":0.0,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbe.4c00083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142020329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycopolypeptide Coordinated Nanovaccine: Fabrication, Characterization, and Antitumor Immune Response.","authors":"Yingying Song, Lin Teng, Yanzheng Chen, Chang-Ming Dong","doi":"10.1021/cbe.4c00083","DOIUrl":"10.1021/cbe.4c00083","url":null,"abstract":"<p><p>Cancer nanovaccine is a frontier immunotherapy strategy, in which the delivery carrier can protect antigen and adjuvant from degradation, increase blood circulation half-life, and improve antigen permeability and presentation, thus enhancing the security and potency of nanovaccine. To address the barriers of antigen delivery, we design and fabricate a kind of intracellular pH-sensitive glycopolypeptide coordinated nanovaccine (OVA-HPGM-Mn) with ∼30% loading capacity of ovalbumin (OVA). The nanovaccine OVA-HPGM-Mn could specifically deliver antigen to dendritic cells (DCs) and effectively escape from endolysosomes to cytoplasm after 6 h of incubation, while the blank counterpart HPGM-Mn acted as an adjuvant to promote DCs maturation and increase the percentage of maturated cells to 26.5% from 11.8% in vitro. Furthermore, the mannosylated polypeptide nanovaccine prolonged the retention time of OVA for 72 h to facilitate 29.5% DCs maturation in lymph nodes, activated 48.8% CD8⁺T cells in spleen, increased the CD8⁺/CD4⁺T cell ratio twice to 1.06, and upregulated the levels of pro-inflammatory cytokines including TNF-α, IFN-γ, and IL-6, thus inhibiting the tumor growth of ∼80%. Consequently, this work provides a versatile strategy for the fabrication of glycosylated polypeptide coordinated nanomaterials for antigen delivery and cancer immunotherapy.</p>","PeriodicalId":100230,"journal":{"name":"Chem & Bio Engineering","volume":"1 7","pages":"633-646"},"PeriodicalIF":0.0,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11835264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143461411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Antibacterial Polymer Coatings Synthesized via Chemical Vapor Deposition","authors":"Haonian Shu,&nbsp;Pengyu Chen and Rong Yang*,&nbsp;","doi":"10.1021/cbe.4c0004310.1021/cbe.4c00043","DOIUrl":"https://doi.org/10.1021/cbe.4c00043https://doi.org/10.1021/cbe.4c00043","url":null,"abstract":"<p >Biofouling is a major issue across various industries ranging from healthcare to the production of food and water and transportation. Biofouling is often induced or mediated by environmental microbes, such as bacteria. Therefore, developing antibacterial coatings has been an essential focus of recent research on functional polymer thin films. To achieve high film quality, vapor-phase techniques represent promising alternatives to traditional solution-based methods, especially for the design and synthesis of antibacterial polymer coatings, as they enable highly uniform, chemically precise, and substrate-independent coatings. This Perspective examines the potential of vapor-phase polymerization techniques to create novel antibacterial polymer coatings. Current advancements in the design of antifouling, bactericidal, antibiofilm, and multifunctional coatings via vapor-phase techniques are organized based on their action mechanisms and design principles. The opportunities and challenges associated with implementing vapor-phase polymerization for developing antibacterial coatings are highlighted.</p>","PeriodicalId":100230,"journal":{"name":"Chem & Bio Engineering","volume":"1 6","pages":"516–534 516–534"},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbe.4c00043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141959193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering Interphasial Chemistry for Zn Anodes in Aqueous Zinc Ion Batteries","authors":"Boyuan Zhu,&nbsp;Jiahao Tang,&nbsp;Zhenjie Yao,&nbsp;Junjie Cui,&nbsp;Yangrui Hou,&nbsp;Jiarong Chen,&nbsp;Li Tang,&nbsp;Yongsheng Fu,&nbsp;Wenyao Zhang* and Junwu Zhu*,&nbsp;","doi":"10.1021/cbe.4c00053","DOIUrl":"10.1021/cbe.4c00053","url":null,"abstract":"<p >Aqueous zinc ion batteries (AZIBs) have emerged as promising candidates for large-scale energy storage systems during post lithium-ion era, drawing attention for their environmental-friendliness, cost-effectiveness, high safety, and minimal manufacturing constraints. However, the long-standing roadblock to their commercialization lies in the dendrite growth and parasitic reactions (hydrogen evolution reaction and water-induced corrosion) of the metallic zinc anode, which strongly depends on the complicated interphasial chemistries. This review, with a focus on optimizing the zinc anode/electrolyte interphase, begins by elucidating the intrinsic factor of zinc ions’ migration, diffusion, nucleation, electro-crystallization, and growth of the zinc nucleus in AZIBs, along with the underlying scientific principles. Then the electrochemical theories pertinent to the plating behavior of the interphase is systematically clarified, thereby enriching the understanding of how anode structure and electrolyte design principles relate to the electrode interphase. Accordingly, the rational strategies emphasizing structural engineering of the zinc anode and electrolyte have been summarized and discussed in detail. The mechanisms, advances, drawbacks, and future outlook of these strategies are analyzed for the purpose of fabricating a chemically and electrochemically stable interphase. Finally, the challenging perspectives and major directions of zinc anode are proposed. This review is expected to shed light on developing high-performance Zn anodes for use in sustainable AZIBs.</p>","PeriodicalId":100230,"journal":{"name":"Chem & Bio Engineering","volume":"1 5","pages":"381–413"},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbe.4c00053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141349700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances in Metal-Free Peptide Stapling Strategies","authors":"Wanglin Zhan,&nbsp;Hongliang Duan and Chengxi Li*,&nbsp;","doi":"10.1021/cbe.3c0012310.1021/cbe.3c00123","DOIUrl":"https://doi.org/10.1021/cbe.3c00123https://doi.org/10.1021/cbe.3c00123","url":null,"abstract":"<p >Protein–protein interactions (PPIs) pose challenges for intervention through small molecule drugs, protein drugs, and linear peptides due to inherent limitations such as inappropriate size, poor stability, and limited membrane penetrance. The emergence of stapled α-helical peptides presents a promising avenue as potential competitors for inhibiting PPIs, demonstrating enhanced structural stability and increased tolerance to proteolytic enzymes. This review aims to provide an overview of metal-free stapling strategies involving two identical natural amino acids, two different natural amino acids, non-natural amino acids, and multicomponent reactions. The primary objective is to delineate comprehensive peptide stapling approaches and foster innovative ideation among readers by accentuating methodologies published within the past five years and elucidating evolving trends in stapled peptides.</p>","PeriodicalId":100230,"journal":{"name":"Chem & Bio Engineering","volume":"1 7","pages":"593–605 593–605"},"PeriodicalIF":0.0,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbe.3c00123","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142020328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradable Poly(amino acid)–Bismuth Nanotheranostic Agents for CT/MR Imaging and Photothermal–Chemodynamic Synergistic Therapy","authors":"Fengfeng Xiao,&nbsp;Yongkang Liu,&nbsp;Yanhong Su,&nbsp;Xu He,&nbsp;Ligong Lu,&nbsp;Meixiao Zhan,&nbsp;Liewei Wen*,&nbsp;Yunlu Dai* and Bing Liu*,&nbsp;","doi":"10.1021/cbe.4c00078","DOIUrl":"10.1021/cbe.4c00078","url":null,"abstract":"<p >Clearly delineating the tumor foci based on multimodal imaging techniques and precisely guiding the minimally invasive therapy are pivotal to completely remove tumors, especially for early micro-tumor lesions. Nevertheless, single-mode imaging techniques are difficult to accurately visualize the tumor region, and the mono-therapeutic strategy is hardly a complete removal of the tumor. In this study, we prepare a biodegradable amphiphilic polymer containing poly(aspartic acid). It is further self-assembled with Bi³⁺ and ultrasmall Fe₃O₄ to form a multifunctional nanocomplex (Bi/Fe₃O₄@P3), which served as a CT/MRI dual-imaging contrast agent and enhanced the photothermal/chemodynamic synergistic therapy. In addition, to enhance the photothermal efficiency, the thermal stress also elevated the level of intracellular H₂O₂, which would facilitate the Fenton reaction between Bi³⁺/Fe²⁺ and H₂O₂ and improve the chemodynamic therapy (CDT) efficacy. Particularly, Bi/Fe₃O₄@P3 would concurrently deplete the abundant intracellular GSH through the coordination of Bi³⁺ with GSH to further potentiate the PTT/CDT synergistic tumoricidal efficacy. Therefore, our study was expected to provide a promising theranostic nano-agent and potential comprehensive therapeutic strategy for microtumors.</p>","PeriodicalId":100230,"journal":{"name":"Chem & Bio Engineering","volume":"1 5","pages":"448–460"},"PeriodicalIF":0.0,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbe.4c00078","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141373875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}