Carbohydrate Polymer Technologies and Applications最新文献

{"title":"Mannan-rich Holocellulose nanofibers mechanically isolated from spent coffee grounds: Structure and properties","authors":"Noriko Kanai ,&nbsp;Kohei Yamada ,&nbsp;Chika Sumida ,&nbsp;Miyu Tanzawa ,&nbsp;Yuto Ito ,&nbsp;Toshiki Saito ,&nbsp;Risa Kimura ,&nbsp;Miwako Saito-Yamazaki ,&nbsp;Toshiyuki Oyama ,&nbsp;Akira Isogai ,&nbsp;Izuru Kawamura","doi":"10.1016/j.carpta.2024.100539","DOIUrl":"https://doi.org/10.1016/j.carpta.2024.100539","url":null,"abstract":"<div><p>Spent coffee grounds (SCGs) contain abundant polysaccharides consisting of mannose (29 %), galactose (11 %), and glucose (11 %) and are a promising source of holocellulose nanofibers (HCNFs). In this study, the mannan-rich HCNFs were isolated from the SCG holocellulose in the yield of 52 % SCGs using an ultrahigh-pressure wet jet mill. The HCNF was refined by passing the suspension through a high-pressure homogenizer with a 95 <em>μ</em>m nozzle 1–15 times. The 5-pass HCNFs were 2.4 nm wide and 0.7 <em>μ</em>m long with 143 viscosity-average degrees of polymerization and contained mannan I crystals (5–10 nm in size) on cellulose microfibrils. The delignification process in water at 75 °C based on the Wise method allowed recrystallization of mannan on a cellulose microfibril substrate. The once-freeze-dried HCNFs had 30–50 nm widths and were not fully nanofibrillated in water when shaking the HCNF/water suspensions but exhibited comparable viscosities to those prepared by mechanical milling. The SCGs-derived HCNFs have a high potential for application in the food industry.</p></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100539"},"PeriodicalIF":6.2,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666893924001191/pdfft?md5=ed8ac245f19be1965c69dad406a765de&pid=1-s2.0-S2666893924001191-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141487129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility study on the application of pectin as pollen adsorbent to prevent pollinosis","authors":"Chihiro Ozawa ,&nbsp;Chika Iwami ,&nbsp;Yayoi Kawano ,&nbsp;Takehisa Hanawa","doi":"10.1016/j.carpta.2024.100538","DOIUrl":"https://doi.org/10.1016/j.carpta.2024.100538","url":null,"abstract":"<div><p>In this study, to investigate the feasibility of applying pectin as an adsorbent to prevent pollinosis, we focused on the two types, low- and high‑methoxy pectin, and investigated its properties for application in nasal mucosa-protective preparations. A film adhesion test was performed, and the number of fluorescent microspheres (FMs) adsorbed to the pectin layer was prepared using a hydrophilic polyvinylidene fluoride filter, and mucin was quantified by fluorescence. High‑methoxy pectin (HMP) caught more FMs than low‑methoxy pectin (LMP)(<em>p</em> &lt; 0.05). The quartz crystal microbalance with dissipation monitoring revealed that HMP adhered more to mucin than LMP. The concentration of HMP did not affect adhesiveness. Furthermore, from evaluating the adsorbency of FMs on pectin and mucin, HMP shows higher adsorbency than LMP (<em>p</em> &lt; 0.05). From these results, it could be concluded that pectin with an HMP had stronger interactions with mucin than LMP and could be applied to the nasal formulation to prevent pollinosis.</p></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100538"},"PeriodicalIF":6.2,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266689392400118X/pdfft?md5=6a1c6aa5c28a1f8cd64b4b4701d997d6&pid=1-s2.0-S266689392400118X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141487132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of chitosan and its modified derivatives as vaccine adjuvant: A review","authors":"Girish Kumar ,&nbsp;Tarun Virmani ,&nbsp;Shashi Kiran Misra ,&nbsp;Ashwani Sharma ,&nbsp;Kamla Pathak","doi":"10.1016/j.carpta.2024.100537","DOIUrl":"https://doi.org/10.1016/j.carpta.2024.100537","url":null,"abstract":"<div><p>Chitosan is a natural polysaccharide that has earned much credit in the pharmaceutical field due to its broad implementation in drugs, antigens, and vaccine delivery. Owing to unique characteristics of biocompatibility, biodegradability, mucoadhesiveness, and less toxicity, chitosan holds promise as a vaccine adjuvant or vaccine delivery system. Furthermore, chitosan exhibits antiviral properties making it an ideal vaccine adjuvant and carrier for the delivery of vaccine. The presence of ridiculous functional groups in the parent structure of chitosan enables the creation of modified chitosan derivatives having improved physiochemical properties which provide enhanced vaccine adjuvant properties of chitosan. This review summarizes the potential of chitosan and its modified derivatives as an antiviral agent and vaccine adjuvant along with patents related to the application of chitosan as a vaccine adjuvant and vaccine carrier.</p></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100537"},"PeriodicalIF":6.2,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666893924001178/pdfft?md5=ef70fc6dec04696462382d303129f149&pid=1-s2.0-S2666893924001178-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141487130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanocellulose derived from agricultural biowaste by-products–Sustainable synthesis, biocompatibility, biomedical applications, and future perspectives: A review","authors":"Md. Meraj Ansari ,&nbsp;Yunji Heo ,&nbsp;Kyoungtag Do ,&nbsp;Mrinmoy Ghosh ,&nbsp;Young-Ok Son","doi":"10.1016/j.carpta.2024.100529","DOIUrl":"https://doi.org/10.1016/j.carpta.2024.100529","url":null,"abstract":"<div><p>Cellulose, a natural linear biopolymer composed of hierarchically arranged cellulose nanofibrils, presents a compelling avenue for sustainable nanocellulose synthesis from agricultural by-products. This innovative approach both mitigates organic waste and landfill disposal and unlocks the latent potential of nanocellulose, transforming agricultural residue into valuable resources. This paradigm shift towards sustainability resonates across diverse industrial sectors, particularly in biomedical research and development. In recent years, the remarkable attributes of nanocellulose, including its biocompatibility, low cytotoxicity, and exceptional water-holding capacity for cell immobilization, have propelled its adoption in various medical applications. From drug delivery systems to wound healing, tissue engineering, and antimicrobial treatments, nanocellulose has emerged as a versatile biomaterial. Moreover, the strategic integration of nanocellulose into composites and its structural functionalization enable customizing its properties for specific functions, further expanding its utility. This comprehensive review explores prominent types of nanocellulose—including cellulose nanocrystals, cellulose nanofibrils, and microbial or bacterial cellulose—elucidating their biomedical applications. This review underscores the sustainability principles underpinning its utilization by exploring the cellulose sources derived from biowaste and industrial processes for nanocellulose production. As a crucial component in a wide array of biomedical materials, nanocellulose both drives innovation and propels the advancement of biomedicine toward sustainability.</p></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100529"},"PeriodicalIF":6.2,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666893924001099/pdfft?md5=3ed02a08dd06df7da0398b1223b6e33d&pid=1-s2.0-S2666893924001099-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141487131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low molecular weight chitosan (∼20 kDa) exhibits in vivo anti-hyperglycemic effects through AKT/PI3K/FOXO pathway","authors":"Priyanka D․ N․,&nbsp;Harish Prashanth K․ V․","doi":"10.1016/j.carpta.2024.100534","DOIUrl":"https://doi.org/10.1016/j.carpta.2024.100534","url":null,"abstract":"<div><p>Diabetes, a metabolic syndrome, is a leading global cause of morbidity and mortality. Dietary polysaccharide intervention besides medication, helps in diabetes through managing blood glucose levels. The present study elucidates the anti-hyperglycemic mechanism of water-soluble LMWC (∼20 kDa), the chitosan derivative, in type-1 diabetic (T1DM) male Wistar rats induced by streptozotocin (STZ, 36 mg/kg b.w.). LMWC1 (100 mg/kg b.w.) and LMWC2 (250 mg/kg b.w.) were administered orally on alternative days for 4 weeks. LMWC lowered fasting blood glucose levels and improved symptoms like polyphagia, polydipsia, polyuria, and serum insulin levels in a dose-dependent manner. It also ameliorated hyperglycemia &amp; dyslipidemia by mitigating the levels of pro-inflammatory factors and oxidative stress levels in the pancreas and liver. Additionally, LMWC increased the activity of key proteins involved in insulin signaling in these organs through the AKT/PI3K/FOXO pathway, enhancing insulin secretion and improving glucose metabolism in T1DM rats. This contributed to improved hepatic glycogen synthesis and suppression of gluconeogenesis in T1DM rats. In summary, LMWC could be an effective antidiabetic supplement, offering insights into dietary treatment for diabetes management.</p></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100534"},"PeriodicalIF":6.2,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666893924001142/pdfft?md5=fcd5abbd370cc5a44d5f1394b7fcfbeb&pid=1-s2.0-S2666893924001142-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141438941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of silver nanoparticles (AgNPs) synthesized using polyphenolic compounds from Phyllanthus emblica L. and their impact on cytotoxicity in human cell lines","authors":"Azhagu Madhavan Sivalingam ,&nbsp;Arjun Pandian","doi":"10.1016/j.carpta.2024.100535","DOIUrl":"https://doi.org/10.1016/j.carpta.2024.100535","url":null,"abstract":"<div><p>The <em>Phyllanthus emblica</em> L. highly medicinal value used for jaundice, anticancer, antioxidant, antiviral, antimicrobial etc., activities so for that current research explored the application of nanotechnology in developing anticancer strategies through the synthesis of silver nanoparticles (AgNPs) using natural extracts from leaves. Various characterization techniques were employed to assess the anticancer properties against the human MCF-7 Breast Cancer Cell Line. This study in UV‒visible spectroscopy AgNPs detected at 436 nm, FT-IR analysis indicated the presence of specific functional groups with high peak intensity at 3105 cm⁻¹. In SEM measurements revealed synthesized AgNPs at 25.4 nm, TEM exposed perfectly round particles with a lattice spacing of 0.295 nm, and XRD analysis revealed reflections at (111), (200), (220), and (310) with corresponding 2θ values of 39.1°, 46.1°, 68.7°, and 79.2°, respectively, along with the observation of (311). EDX analysis confirmed the composite nature of quartz with AgNPs at a 50:50 ratio. Atomic force microscopy (AFM) images of AgNPs 30 nm in size. Furthermore, breast cancer one of the major disease, targeted application of our study focused anticancer activity against MCF-7 breast cancer cell line, observed that plant extract exhibits 92% cancer cell death at 500 µg/ml, showed potential anticancer activity against breast cancer cells. In flowcytometry cancer cells phase distribution was observed at AgNPs (100 to 200 µg/ml). Prolonged exposure to the extract led to cell death and arrested the apoptotic cell death cycle after 24 hours. Research has also evaluated the potential of nanoparticles to inhibit cancer progression, revealing promising prospects for enhancing anticancer therapies. During the treatment against cancer cells observed cell deaths, concluded that green, nanotechnology potential tool for modern world.</p></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100535"},"PeriodicalIF":6.2,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666893924001154/pdfft?md5=dc385c684413016e6b218740ed8cd3ec&pid=1-s2.0-S2666893924001154-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141434060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the mechanical strength and tuning the optical properties of reinforced PVA films: The effects of graphene oxide, cellulose nanocrystals, and PVA molecular weight","authors":"Duc-Ninh Lam ,&nbsp;Nhung Tuyet Thi Nguyen ,&nbsp;Doan Van Hong Thien ,&nbsp;Chanh-Nghiem Nguyen ,&nbsp;Chun-Jen Huang ,&nbsp;Dan-Thuy Van-Pham","doi":"10.1016/j.carpta.2024.100533","DOIUrl":"https://doi.org/10.1016/j.carpta.2024.100533","url":null,"abstract":"<div><p>This study investigates the synergy of varying concentrations of graphene oxide (GO) and cellulose nanocrystals (CNCs), and poly(vinyl alcohol) (PVA) molecular weight (MW) on the UV protection, transparency, and tensile strength (TS) of PVA/GO/CNC films. GO and CNCs were sustainably synthesized from graphite and office waste paper. PVA MW ranged from 20 to 130 kDa for broad applicability. The maximum percent of TS improvement (POTSI) reached 40% under 65% relative humidity, influenced more by CNC and GO concentrations than PVA MW. GO significantly impacted the films' optical properties. For low PVA MW (20 kDa), the optimized film (1.00 wt% GO, 3.52 wt% CNC) achieved excellent UVA (82.3%) and UVB (91.7%) barriers, acceptable transparency (43.9%), and a POTSI of 33.5%. For high PVA MW (130 kDa), the optimized film (0.6 wt% GO, 1.86 wt% CNC) exhibited higher transparency (50.8%) but reduced UVA (69.2%) and UVB (77.9%) barriers, and a POTSI of 36.5%. UV barriers can be controllably improved by increasing GO concentration, followed by CNC adjustment to preserve TS. Conversely, transparency can be enhanced by reducing GO and CNC concentrations, with some compromise in UV protection. These findings guide the optimization of PVA/GO/CNC films for enhanced performance in the composite industry.</p></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100533"},"PeriodicalIF":6.2,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666893924001130/pdfft?md5=ecd1bfcb9566d728f587e79ed9562d57&pid=1-s2.0-S2666893924001130-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan-derivative nanoparticles loaded with dihydromyricetin: Characterization, antibacterial and antioxidant activities","authors":"Chenmiao Zhang,&nbsp;Shijiao Guo,&nbsp;Lihua Ye,&nbsp;Jinjin Tong ,&nbsp;Linshu Jiang","doi":"10.1016/j.carpta.2024.100532","DOIUrl":"10.1016/j.carpta.2024.100532","url":null,"abstract":"<div><p>Bovine mastitis is a global problem and is mainly treated with antimicrobial agents that can significantly affect milk quality and threaten human health. The mammary gland can be damaged by the accumulation of reactive oxygen species (ROS), particularly during the perinatal period. Dihydromyricetin (DHM) has a wide range of pharmacological activities and is considered safe. However, its poor solubility, absorption, and bioavailability have limited its application. To address this issue, we designed and synthesized new chitosan-derivative nanoparticles (HACC-NPs) loaded with DHM, which have the potential to overcome its bioabsorption problems and enhance its antibacterial and antioxidant effects. We used the emulsification/crosslinking method to load DHM into chitosan-derivative nanoparticles (DHM/HACC-NPs); then, SEM, TEM and FTIR analyses were used to show a denser, more tightly packed matrix for the DHM/HACC-NPs, which were successfully prepared. The size (176.4 nm), polydispersity index (PDI, 0.21), and absolute value of the zeta potential (+26.3 mV) confirmed the stability of the DHM/HACC-NPs. More importantly, DHM/HACC-NPs had a stronger antibacterial effect on <em>S. aureus</em> (MIC = 0.52 mg/mL and MBC = 1.02 mg/mL) than on <em>E. coli</em> (MIC = 1.02 mg/mL and MBC = 2.05 mg/mL). Notably, DHM/HACC-NPs were concentration-dependent scavengers of DPPH and hydroxyl radicals. In summary, DHM/HACC-NPs exhibited superior biocompatibility and antimicrobial and antioxidant activities, offering new insights into the development of a robust, udder-stable drug platform as a promising strategy for treating dairy cow mastitis.</p></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100532"},"PeriodicalIF":0.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666893924001129/pdfft?md5=857975fd93cf20942e05301e32cac19a&pid=1-s2.0-S2666893924001129-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141395359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan nanoparticle encapsulation of celery seed essential oil: Antifungal activity and defense mechanisms against cucumber powdery mildew","authors":"Hajar Soleimani ,&nbsp;Reza Mostowfizadeh-Ghalamfarsa ,&nbsp;Afsaneh Zarei","doi":"10.1016/j.carpta.2024.100531","DOIUrl":"10.1016/j.carpta.2024.100531","url":null,"abstract":"<div><p>The objective of the current study was to explore the antifungal potential of celery seed essential oil (CSEO) in a nano-carrier system against <em>Podosphaera fusca,</em> the causal agent of cucumber powdery mildew. We assessed the physicochemical characterization of CSEO-loaded chitosan nanoparticles (CSEO-LCNPs) using dynamic light scattering, field emission scanning electron microscopy, and Fourier transform infrared spectroscopy. The successful loading of various concentrations of CSEO into chitosan nanoparticles (1:0, 1:0.25, 1:0.5, 1:0.75, and 1:1) has been verified. The particles exhibited a spherical shape, with an average diameter of 113 nm, and a negatively charged surface with a zeta potential of -31.5 mV. The Korsmeyer-Peppas model well described the release behavior of CSEO from nanoparticles. Additionally, we examined the defense mechanism, as well as biochemical/molecular responses following foliar treatment with CSEO-LCNPs. <em>In vivo</em>, results revealed that CSEO-LCNPs foliar application on the cucumber seedlings inoculated with <em>P. fusca</em> significantly reduced the disease severity of powdery mildew. Treatment with CSEO-LCNPs led to an increase in phenolic and flavonoid compounds, chlorophyll concentration, enzyme activities, and gene transcriptions in inoculated cucumber seedlings. In our study, we observed a positive correlation between antioxidant compounds and the defense-associated enzymes as well as their corresponding gene expressions. Our study underscores the potential of CSEO-LCNPs as an eco-friendly and effective solution for managing cucumber powdery mildew.</p></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100531"},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666893924001117/pdfft?md5=ecf600aa82484fd29b24821e3bf84eb8&pid=1-s2.0-S2666893924001117-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141399059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant protection of a polysaccharide produced by Chaetomium globosum CGMCC 6882 on H2O2-challenged HepG2 cells","authors":"Zichao Wang ,&nbsp;Yi Zheng ,&nbsp;Wenzhuo Lu ,&nbsp;Jinchu Yang ,&nbsp;Yingjie Feng ,&nbsp;Zhitao Li ,&nbsp;Na Li ,&nbsp;Yahui Yang ,&nbsp;Qi Wang ,&nbsp;Lemei An","doi":"10.1016/j.carpta.2024.100530","DOIUrl":"https://doi.org/10.1016/j.carpta.2024.100530","url":null,"abstract":"<div><p>Effective utilization of waste kitchen oil (WKO) is important to environmental protection and economic development. Presently, an endophytic fungus <em>Chaetomium globosum</em> CGMCC 6882 polysaccharide (CGP-WKO) was successfully produced through submerged fermentation with the sole carbon source of WKO. CGP-WKO had a yield of 1.53 ± 0.27 g/L and contained 95.85 % ± 3.02 % carbohydrate and 2.94 % ± 0.62 % protein. Structural feature analysis indicated that CGP-WKO contained glucose, glucosamine, mannose, rhamnose, galactose, fructose, and glucuronic acid in a molar ratio of 30.38: 1.34: 32.22: 9.68: 1.59: 0.62: 3.73. The weight-averaged molecular weight of CGP-WKO was 26.64 kDa, and its polydispersity was 1.48. Moreover, antioxidant capacity detection <em>in vitro</em> demonstrated that when the concentration of CGP-WKO was 1.0 mg/mL, its scavenging effects against 1,1-diphenyl-2-picrylhydrazyl; 2,2′-azinobis-di-(3-ethyl-benzothiazolin-6-sulfonic acid) diammonium salt; and hydroxyl and superoxide radicals were 45.89 % ± 1.89 %, 55.89 % ± 1.12 %, 29.02 % ± 2.61 %, and 52.76 % ± 2.12 %, respectively; and the IC₅₀ values were 6.4612, 0.8888, 13.8585, and 0.9398 mg/mL, respectively. CGP-WKO increased the content of superoxide dismutase, catalase, and glutathione peroxidase and reduced malondialdehyde content in H₂O₂-challenged HepG2 cells. Overall, the present work indicated that CGP-WKO can be used as an antioxidant agent in the food, cosmetics, and pharmaceutical industries.</p></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100530"},"PeriodicalIF":0.0,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666893924001105/pdfft?md5=ce2064058354f59ff9fb5f6a45a987f5&pid=1-s2.0-S2666893924001105-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141323307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}