Brain Organoid and Systems Neuroscience Journal最新文献_第6页

Biomimetic in vitro heart platforms for drug development 用于药物开发的体外仿生心脏平台

Brain Organoid and Systems Neuroscience Journal Pub Date : 2022-02-16 DOI: 10.51335/organoid.2022.2.e1

Sungwoo Cho, S. Ko

引用次数: 0

Recent advances in liver organoids and their use in in vitro modeling of non-alcoholic fatty liver disease 肝类器官的研究进展及其在非酒精性脂肪性肝病体外模型中的应用

Brain Organoid and Systems Neuroscience Journal Pub Date : 2022-01-25 DOI: 10.51335/organoid.2022.2.e6

Kyun Yoo Chi, Jong-Hoon Kim

{"title":"Recent advances in liver organoids and their use in in vitro modeling of non-alcoholic fatty liver disease","authors":"Kyun Yoo Chi, Jong-Hoon Kim","doi":"10.51335/organoid.2022.2.e6","DOIUrl":"https://doi.org/10.51335/organoid.2022.2.e6","url":null,"abstract":"The liver is involved in physiological activities critical for survival. Chronic liver disease (CLD) frequently progresses to life-threatening liver failure, as evidenced by CLD patients’ high morbidity and mortality rates. Over the years, non-alcoholic fatty liver disease (NAFLD) has received significant attention as an etiology of CLD given its increasing prevalence and progression towards severe pathological conditions such as fibrosis. To answer the urgent need for effective therapeutics that treat CLD, an advanced cellular model, the liver organoid, is used to model and study the complex pathophysiology of NAFLD. Liver organoids recapitulate in vivo aspects of liver tissue such as 3-dimensional cell-cell and cell-extracellular matrix interactions and biomolecular gradients. Moreover, liver organoids can be readily generated from patient-specific liver tissues and induced pluripotent stem cells, enabling their use in a wide range of personalized clinical applications. In recent research, numerous attempts have been made to establish multicellular liver organoids capable of modeling disease phenotypes that involve parenchymal and non-parenchymal liver cells. In this review, we focus on recent advances in liver organoids and highlight the applicability of organoids for modeling NAFLD within the context of cellular sources and composition.","PeriodicalId":100198,"journal":{"name":"Brain Organoid and Systems Neuroscience Journal","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82565643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The application of intestinal organoids and their co-culture systems in the study of gastrointestinal diseases 肠道类器官及其共培养系统在胃肠道疾病研究中的应用

Brain Organoid and Systems Neuroscience Journal Pub Date : 2022-01-15 DOI: 10.51335/organoid.2022.2.e3

Panida Sittipo, Y. Lee

引用次数: 1

A novel method for the isolation of CD45-positive and CD45-negative cells from malignant pleural effusion 一种分离恶性胸腔积液cd45阳性和cd45阴性细胞的新方法

Brain Organoid and Systems Neuroscience Journal Pub Date : 2021-12-15 DOI: 10.51335/organoid.2021.1.e13

Jimin Choi, Jeong Uk Lim, Wooil Kim, Dong Woo Lee, S. Kwon, Sang-Hyun Lee

{"title":"A novel method for the isolation of CD45-positive and CD45-negative cells from malignant pleural effusion","authors":"Jimin Choi, Jeong Uk Lim, Wooil Kim, Dong Woo Lee, S. Kwon, Sang-Hyun Lee","doi":"10.51335/organoid.2021.1.e13","DOIUrl":"https://doi.org/10.51335/organoid.2021.1.e13","url":null,"abstract":"Background: Patient-derived malignant pleural effusion (MPE) samples can be used to identify a patient-specific drug combination since MPE samples are readily available and cost-effective tumor cell sources. However, the isolation of target cancer cells from MPE has been inefficient because MPE samples contain a complex mixture of immune cells, non-cancerous cells, and cancer cells. Hence, new methods need to be developed to effectively isolate target cancer cells from MPE samples that can be used for 3-dimensional (3D) cell culture. Patient-derived in vitro 3D tumor models are expected to facilitate more precise drug treatment.Methods: MPE samples were obtained from Seoul St. Mary’s Hospital, The Catholic University of Korea with consent from patients previously diagnosed with lung adenocarcinoma. We isolated target cells from MPE samples using 2 different Percoll-gradient centrifugation methods.Results: The use of 40% and 75% Percoll-gradient centrifugation led to a clearer separation of CD45-positive (CD45pos) and CD45-negative (CD45neg) cells than the traditional 44% and 67% Percoll-gradient centrifugation method.Conclusion: Our findings strongly suggest that the 40% and 75% Percoll-gradient centrifugation method is more useful for the isolation of CD45pos or CD45neg cells than the previously described Percoll-gradient centrifugation method. Furthermore, our novel method was useful for the isolation of MPE-derived target cancer cells that can be used to construct in vitro patient-specific 3D tumor models.","PeriodicalId":100198,"journal":{"name":"Brain Organoid and Systems Neuroscience Journal","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85946479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bronchioalveolar organoids as a tool to study transforming growth factor-β and cigarette smoke–induced lung pathology 细支气管肺泡类器官作为研究转化生长因子-β和香烟引起的肺部病理的工具

Brain Organoid and Systems Neuroscience Journal Pub Date : 2021-11-15 DOI: 10.51335/organoid.2021.1.e12

S. Jeong, Jung-Hyun Kim, Roya Rasaei, Seok-Ho Hong

{"title":"Bronchioalveolar organoids as a tool to study transforming growth factor-β and cigarette smoke–induced lung pathology","authors":"S. Jeong, Jung-Hyun Kim, Roya Rasaei, Seok-Ho Hong","doi":"10.51335/organoid.2021.1.e12","DOIUrl":"https://doi.org/10.51335/organoid.2021.1.e12","url":null,"abstract":"Respiratory medicine has high barriers to new drug development, with fewer approved new treatments and candidate drugs and a higher failure rate than other common disease fields. Most of the major candidate drugs identified in preclinical animal studies fail in the clinical setting because of differences between animal models and humans. Therefore, the rapid development of 3-dimensional (3D) organoid-based disease models that recapitulate human pathological development has attracted increasing attention in drug development and personalized medicine. In the present study, we generated bronchoalveolar organoids (BAOs) from human pluripotent stem cells (hPSCs) and assessed their potential as a pulmonary disease model. Derived BAOs contained the expected spectrum of differentiated cells, including alveolar progenitors, type 1 and 2 alveolar epithelial cells, basal cells, secretory cells, ciliated cells, and mesenchymal cells. When the BAOs were exposed to transforming growth factor-beta, both fibrosis- and inflammation-related transcripts were significantly upregulated compared to the control. In addition, the exposure of BAOs to cigarette smoking extract induced increased levels of nitric oxide in a dose-dependent manner, as well as upregulating oxidative stress-related and pro-inflammatory genes. These findings suggest that hPSC-derived BAOs could be a promising platform for modeling pulmonary fibrosis and chronic obstructive pulmonary disease and testing drug efficacy.","PeriodicalId":100198,"journal":{"name":"Brain Organoid and Systems Neuroscience Journal","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82886737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell transcriptomics of lung organoids 肺类器官的单细胞转录组学

Brain Organoid and Systems Neuroscience Journal Pub Date : 2021-10-19 DOI: 10.51335/ORGANOID.2021.1.E9

Y. Song, Man Ryul Lee

引用次数: 0

Strategies for vascularization in kidney organoids 肾类器官血管化策略

Brain Organoid and Systems Neuroscience Journal Pub Date : 2021-08-15 DOI: 10.51335/organoid.2021.1.e14

SeonJu Park, Yong kyun Kim

引用次数: 0

Trends in the global organoid technology and industry: from organogenesis in a dish to the commercialization of organoids 全球类器官技术和产业的趋势:从培养皿中的器官生成到类器官的商业化

Brain Organoid and Systems Neuroscience Journal Pub Date : 2021-07-10 DOI: 10.51335/organoid.2021.1.e11

Hanbyeol Lee, Jeong Suk Im, Daejin Choi, Dong-Hun Woo

{"title":"Trends in the global organoid technology and industry: from organogenesis in a dish to the commercialization of organoids","authors":"Hanbyeol Lee, Jeong Suk Im, Daejin Choi, Dong-Hun Woo","doi":"10.51335/organoid.2021.1.e11","DOIUrl":"https://doi.org/10.51335/organoid.2021.1.e11","url":null,"abstract":"Animal models have been standard methods for non-clinical research in drug development for decades. However, many drugs that have shown satisfactory results in non-clinical studies have failed in the clinical stage, presumably because animal data are not fully convertible to human data. Human organoid technology has recently been considered as an alternative to existing non-clinical testing methods, and it could potentially serve a role as a bridge from non-clinical to clinical trials, compensating for the current limitations arising from non-clinical animal models. For this reason, organoid technology is being utilized in various fields of research including academic studies, disease modeling, drug screening, biobanks, and regenerative medicine. In addition, as organoid technology progressively develops, it has been combined with bioengineering to develop applications from manufacturing to drug evaluation platforms, which is leading to a demand for commercialization of organoids for researchers. In accordance with this global trend, the organoid industry continues to grow throughout the world, and organoid research and the market for organoids have been boosted by the demand for efficient and rapid drug development in response to the coronavirus disease 2019 pandemic. In this review, we discuss recent global trends in organoid research, based on tissue types and applications, as well as the organoid market and its prospects.","PeriodicalId":100198,"journal":{"name":"Brain Organoid and Systems Neuroscience Journal","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82224679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Engineered models for studying blood-brain-barrier-associated brain physiology and pathology 用于研究血脑屏障相关的脑生理学和病理学的工程模型

Brain Organoid and Systems Neuroscience Journal Pub Date : 2021-06-19 DOI: 10.51335/ORGANOID.2021.1.E10

H. Kim

引用次数: 2

Optimizing a three-dimensional spheroid clearing method for the imaging-based evaluation of cardiotoxicity 优化一种三维球体清除方法，用于基于成像的心脏毒性评估

Brain Organoid and Systems Neuroscience Journal Pub Date : 2021-05-30 DOI: 10.51335/organoid.2021.1.e7

Ji Hye Park, Jaemeun Lee, Sun-Hyun Park, Ki-Suk Kim

{"title":"Optimizing a three-dimensional spheroid clearing method for the imaging-based evaluation of cardiotoxicity","authors":"Ji Hye Park, Jaemeun Lee, Sun-Hyun Park, Ki-Suk Kim","doi":"10.51335/organoid.2021.1.e7","DOIUrl":"https://doi.org/10.51335/organoid.2021.1.e7","url":null,"abstract":"Toxicity evaluation based on two-dimensional cell culture shows differences from clinical results and has the disadvantage of not accurately reflecting cell-to-cell cross-signaling. Since almost all cells in the human body are arranged in a three-dimensional structure and constitute a tissue, the in vitro reproduction of three-dimensional tissues composed of human cells can be used as effective models for drug development and toxicity evaluation. The clearing technique improves image resolution and can be used to generate three-dimensional bio-images throughout the organized structure, improving the efficiency of toxicity evaluation for disease models using spheroids. Herein, we report the first optical spheroid clearing protocol for an image-based toxicity prediction model. In our results, spheroid clearing significantly increased fluorescence intensity and enabled image-based toxicity prediction. We propose that this spheroid clearing method can be utilized for image-based cardiotoxicity evaluation. Furthermore, we also present the possibility that our protocol can be utilized for patient-tailored toxicity prediction.","PeriodicalId":100198,"journal":{"name":"Brain Organoid and Systems Neuroscience Journal","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81953895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0