Brain Organoid and Systems Neuroscience Journal最新文献_第5页

Disease modeling in organoid cultures: a new tool for studying viruses 类器官培养中的疾病建模:研究病毒的新工具

Brain Organoid and Systems Neuroscience Journal Pub Date : 2022-07-25 DOI: 10.51335/organoid.2022.2.e15

Jaewon Cho, Eun-Hye Hong, Hyun-Jeong Ko

{"title":"Disease modeling in organoid cultures: a new tool for studying viruses","authors":"Jaewon Cho, Eun-Hye Hong, Hyun-Jeong Ko","doi":"10.51335/organoid.2022.2.e15","DOIUrl":"https://doi.org/10.51335/organoid.2022.2.e15","url":null,"abstract":"In vitro experiments have been widely used for more than a century to elucidate molecular mechanisms in cells and pathogen-host interactions, as well as for drug screening. Cell lines have been modified to reflect researchers’ specific purposes, and in vitro experiments have become fundamental for biological studies, with an ability to replace in vivo experiments. However, immortalized cell lines and cancer-derived cell lines have the limitation of losing their inherent properties, potentially resulting in changes in signaling pathways and cell metabolism. These limitations have made it necessary for researchers to find a novel way to overcome the limitations of cell lines. In recent years, organoids, which are 3-dimensional multicellular in vitro tissue constructs that fundamentally imitate in vivo organs, have been developed as alternative systems to study various aspects of organs. Herein, we review recent studies on the application of organoids in disease modeling, with a focus on intestine, lung, and tonsil organoids. These 3 organoids have been of utmost interest to researchers since their initial development. Most importantly, organoids are novel experimental models that can simulate in vivo organs and can therefore replace or support existing in vitro and in vivo models.","PeriodicalId":100198,"journal":{"name":"Brain Organoid and Systems Neuroscience Journal","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75351411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the cellular pathogenesis of COVID-19 symptoms using organoid technology 利用类器官技术了解COVID-19症状的细胞发病机制

Brain Organoid and Systems Neuroscience Journal Pub Date : 2022-06-25 DOI: 10.51335/organoid.2022.2.e16

Y. Che, Yong Jun Kim

引用次数: 0

Applications of human brain organoids 人脑类器官的应用

Brain Organoid and Systems Neuroscience Journal Pub Date : 2022-06-25 DOI: 10.51335/organoid.2022.2.e13

A. Muotri

引用次数: 1

Three-dimensional cardiac organoid formation accelerates the functional maturation of human induced pluripotent stem cell-derived cardiomyocytes 三维心脏类器官的形成加速了人诱导多能干细胞衍生的心肌细胞的功能成熟

Brain Organoid and Systems Neuroscience Journal Pub Date : 2022-05-25 DOI: 10.51335/organoid.2022.2.e14

Hanbyeol Lee, Jeong Suk Im, Daejin Choi, Jieun An, Subin Kim, Seunghee Yeon, Seulgi Yoon, Dong-Hun Woo

{"title":"Three-dimensional cardiac organoid formation accelerates the functional maturation of human induced pluripotent stem cell-derived cardiomyocytes","authors":"Hanbyeol Lee, Jeong Suk Im, Daejin Choi, Jieun An, Subin Kim, Seunghee Yeon, Seulgi Yoon, Dong-Hun Woo","doi":"10.51335/organoid.2022.2.e14","DOIUrl":"https://doi.org/10.51335/organoid.2022.2.e14","url":null,"abstract":"Human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (CMs) offer a promising source for heart regeneration, disease modeling, and drug screening. Recent developments in organoid technology have made it possible to study how hiPSC-derived CMs interact together, and this culture system mimics the tissue environment and behavior of the cardiac cells in our body. However, the similarities and differences between conventional 2-dimensional (2D) culture and 3-dimensional (3D) organoid culture systems for CM differentiation have been incompletely elucidated. To study how the individual microenvironment formed by each culture system affects the properties of CMs differentiated from hiPSCs, we conducted a comparative study between 2D monolayer and direct 3D cardiac organoid (hiCO) differentiation from hiPSCs throughout the sequential differentiation stages. Although identical differentiation cues were applied to hiPSCs, the 3D differentiation system strongly exhibited higher mesoderm commitment and cardiac induction than 2D monolayer differentiation. In the late stage of differentiation, the 3D hiCOs showed a higher frequency of a mature myofibrillar isoform switching in sarcomere structure of differentiated CMs than was observed in monolayer culture, although over 94% of cardiac troponin T-positive cells resulted at the end point of differentiation in both systems. Furthermore, the accelerated structural maturation in 3D hiCOs resulted in increased expression of cardiac-specific ion channel genes and Ca2+ transient properties, with a high signal amplitude and rapid contractility. The present study provides details surrounding the 2D and 3D culture methods for CM differentiation from iPSCs, and focuses on 3D cell culture as an improved strategy for approaching and applying cardiac maturation.","PeriodicalId":100198,"journal":{"name":"Brain Organoid and Systems Neuroscience Journal","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79147077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Standard operating protocol of hepatic organoid differentiation from human induced pluripotent stem cells 人诱导多能干细胞向肝类器官分化的标准操作规程

Brain Organoid and Systems Neuroscience Journal Pub Date : 2022-05-15 DOI: 10.51335/organoid.2022.2.e5

Min Jung Kim, Jaeseo Lee, Seon Ju Mun, M. Son, Jung-Hyun Kim

引用次数: 0

Progress, prospects, and limitations of organoid technology 类器官技术的进展、前景与局限性

Brain Organoid and Systems Neuroscience Journal Pub Date : 2022-04-25 DOI: 10.51335/organoid.2022.2.e9

Jin Hee Park, Do Gyeung Byeun, Jung Kyu Choi

引用次数: 2

Recent advances and challenges in organoid-on-a-chip technology 类器官芯片技术的最新进展与挑战

Brain Organoid and Systems Neuroscience Journal Pub Date : 2022-04-15 DOI: 10.51335/organoid.2022.2.e4

Intan Rosalina Suhito, Tae-Hyung Kim

{"title":"Recent advances and challenges in organoid-on-a-chip technology","authors":"Intan Rosalina Suhito, Tae-Hyung Kim","doi":"10.51335/organoid.2022.2.e4","DOIUrl":"https://doi.org/10.51335/organoid.2022.2.e4","url":null,"abstract":"Conventional 2-dimensional cell culture poorly mimics human-relevant models, which is considered a major challenge in biological research. Organoids are a recent breakthrough in 3-dimensional (3D) in vitro tissue engineering that better reflect the physiological, morphological, and functional properties of in vivo organs (e.g., brain, heart, kidney, lung, and liver). Consequently, organoids are extensively used in various impactful biomedical applications including organ development, disease modeling, and clinical drug testing. However, organoid technology still has several limitations, including low reproducibility, vascularization, limited nutrient uptake and distribution (affecting the level of organoid maturation), lack of standardization, and intra-clonal variability. Efforts have been made to overcome these shortcomings of organoid culture. Microfluidic technology has successfully facilitated the establishment of organoid-on-a-chip systems, which effectively improve the structural and physiological features of organoids in a controlled manner. This review discusses the recent advances and developments in organoid-on-a-chip technology. We hope that this study will motivate researchers to explore the possible engagement between microfluidic devices and self-assembled 3D cell cultures to leverage the enhanced quality of organoids, which will have favorable impacts on future tissue regeneration and regenerative therapies.","PeriodicalId":100198,"journal":{"name":"Brain Organoid and Systems Neuroscience Journal","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76090375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Fundamental signaling pathways for glioblastoma drug resistance and developing robust organoid models for drug discovery 胶质母细胞瘤耐药的基本信号通路和开发药物发现的强大类器官模型

Brain Organoid and Systems Neuroscience Journal Pub Date : 2022-03-25 DOI: 10.51335/organoid.2022.2.e8

Athira Pratap, Eun‐Jung Lim, I. Kwak, Byoung-San Moon

引用次数: 0

Emerging organoid-based platforms to study salivary gland hypofunction 新兴的基于器官的平台研究唾液腺功能减退

Brain Organoid and Systems Neuroscience Journal Pub Date : 2022-03-24 DOI: 10.51335/organoid.2022.2.e2

Yoojin Seo, Hyung-Sik Kim

引用次数: 0

Glioblastoma organoid technology: an emerging preclinical models for drug discovery 胶质母细胞瘤类器官技术:药物发现的新兴临床前模型

Brain Organoid and Systems Neuroscience Journal Pub Date : 2022-02-25 DOI: 10.51335/organoid.2022.2.e7

D. C. Batara, Shuchang Zhou, Moon-Chang Choi, Sung-Hak Kim

引用次数: 1