Biotechnology Notes最新文献_第7页

Substrate promiscuity of the NdmCDE N7-demethylase enzyme complex NdmCDE n7 -去甲基酶复合物的底物混杂性

Biotechnology Notes Pub Date : 2021-01-01 DOI: 10.1016/j.biotno.2021.05.001

Meredith B. Mock, Shuyuan Zhang, Brianna Pniak, Nicholas Belt, McKenna Witherspoon, Ryan M. Summers

{"title":"Substrate promiscuity of the NdmCDE N7-demethylase enzyme complex","authors":"Meredith B. Mock, Shuyuan Zhang, Brianna Pniak, Nicholas Belt, McKenna Witherspoon, Ryan M. Summers","doi":"10.1016/j.biotno.2021.05.001","DOIUrl":"10.1016/j.biotno.2021.05.001","url":null,"abstract":"<div>Methylxanthines, including caffeine and theophylline, are a class of natural and synthetic compounds with important roles in food, cosmetics, and medicine. These compounds are metabolized by bacteria using five enzymes from the Rieske non-heme iron oxygenase family, NdmABCDE. The NdmCDE complex is responsible for the N7-demethylation of 7-methylxanthine to xanthine and was originally described as being highly specific for 7-methylxanthine. Here, we report that the NdmCDE complex is also active toward theobromine, producing 3-methylxanthine due to N7-demethylation. Minimal activity was observed when the enzyme complex was tested with caffeine or paraxanthine, indicating that the presence of the N1-methyl group significantly inhibits N7-demethylase activity by NdmCDE. We also demonstrated positional promiscuity in the N3-demethylase, NdmB, which is able to carry out N1-demethylation of paraxanthine. The N1-demethylation by NdmB is limited to paraxanthine and was not observed when caffeine or theophylline were assayed. These newly discovered activities were observed when enzymes were overexpressed in E. coli and differ from results with purified enzymes assayed in vitro, indicating that they may behave differently in vivo. Furthermore, these results reveal promiscuity of bacterial N-demethylase enzymes that can be used to engineer new enzymes and bacterial strains for production of high-value methylxanthines.</div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"2 ","pages":"Pages 18-25"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biotno.2021.05.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92662513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Engineered probiotics modulate the endocannabinoid system 工程益生菌调节内源性大麻素系统

Biotechnology Notes Pub Date : 2021-01-01 DOI: 10.1016/j.biotno.2021.08.001

In Young Hwang , Hye Rim Kim , Ryan De Sotto , Matthew Wook Chang

引用次数: 7

Mapping QTLs for important agronomic traits in an Iniadi-derived immortal population of pearl millet 印度源珍珠粟不朽群体重要农艺性状的qtl定位

Biotechnology Notes Pub Date : 2021-01-01 DOI: 10.1016/j.biotno.2021.06.001

Sushil Kumar , Charles Thomas Hash , Govind Singh , T. Nepolean , Rakesh K. Srivastava

{"title":"Mapping QTLs for important agronomic traits in an Iniadi-derived immortal population of pearl millet","authors":"Sushil Kumar , Charles Thomas Hash , Govind Singh , T. Nepolean , Rakesh K. Srivastava","doi":"10.1016/j.biotno.2021.06.001","DOIUrl":"10.1016/j.biotno.2021.06.001","url":null,"abstract":"<div>Pearl millet [Pennisetum glaucum (L.) R. Br.] is a climate-hardy nutricereal and an essential staple for the people living in dry regions. Substantial improvement has been achieved for seed yield stability in pearl millet, the cultivable area under pearl millet reduces. Deployment of early-flowering, bold-seeded and dwarf genotypes in pearl millet is a vital breeding strategy to improve grain production and enhance the adaptability of pearl millet in low-input farms. Therefore, an experiment was performed for mapping agronomically important traits like flowering time (FT), plant height (PH), panicle length (PL), and 1000-grain weight (TGW) in 317 recombinant inbred line (RIL) population derived from ICMS 8511-S1-17-2-1-1-B-P03 × AIMP 92901-S1-183-2-2-B-08 cross. Broad-sense heritability estimates were high to very high, ranging from 0.52 (PL) to 0.86 (PH). FT showed a significant positive correlation with PH. A key QTL for FT was mapped on LG 1, 15 QTLs for PH scattered on 10 chromosomes, five QTLs for PL dispersed on four chromosomes, and two QTLs for TGW spanned linkage groups 3 and 7. One QTL on LG1 was common for FT and PH. Two major QTLs for PH, one each on LG4B/8 cM and LG7/110 cM were detected. The large effect QTL for TGW on LG7 had a phenotypic variance (R2) of 24.3%. The R2 for PH and PL ranged between 5.2 - 24.5% and 5.0–11.5%, respectively. The QTLs mapped for FT and other agronomic traits in the current study can be exploited to develop elite hybrid parental genotypes/cultivars through marker-assisted breeding and genomic selection.</div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"2 ","pages":"Pages 26-32"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biotno.2021.06.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89205898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Driving innovation in biotechnology with Private-Public Partnerships: A Singapore perspective 以公私伙伴关系推动生物技术创新:新加坡视角

Biotechnology Notes Pub Date : 2021-01-01 DOI: 10.1016/j.biotno.2021.10.001

Hui Ling Lee , Konstantinos Vavitsas

引用次数: 2

The need for integrated systems biology approaches for biotechnological applications 对生物技术应用的综合系统生物学方法的需求

Biotechnology Notes Pub Date : 2021-01-01 DOI: 10.1016/j.biotno.2021.08.002

Kumar Selvarajoo

引用次数: 4

Strategy exploration for developing robust lyophilized cell-free systems 开发健壮的无细胞冻干系统的策略探索

Biotechnology Notes Pub Date : 2021-01-01 DOI: 10.1016/j.biotno.2021.08.004

Junzhu Yang , Yuntao Cui , Zhou Cao , Shengnan Ma, Yuan Lu

引用次数: 8

Directed evolution of VanR biosensor specificity in yeast 酵母中VanR生物传感器特异性的定向进化

Biotechnology Notes Pub Date : 2020-01-01 DOI: 10.1016/j.biotno.2020.01.002

Vasil D'Ambrosio , Subrata Pramanik , Kati Goroncy , Tadas Jakočiūnas , David Schönauer , Mehdi D. Davari , Ulrich Schwaneberg , Jay D. Keasling , Michael K. Jensen

{"title":"Directed evolution of VanR biosensor specificity in yeast","authors":"Vasil D'Ambrosio , Subrata Pramanik , Kati Goroncy , Tadas Jakočiūnas , David Schönauer , Mehdi D. Davari , Ulrich Schwaneberg , Jay D. Keasling , Michael K. Jensen","doi":"10.1016/j.biotno.2020.01.002","DOIUrl":"https://doi.org/10.1016/j.biotno.2020.01.002","url":null,"abstract":"<div>Allosterically regulated transcription factors (aTFs) based biosensors from prokaryotes have been widely used to screen large gene libraries, stabilize engineered microbes from evolutionary drifting, and for detection of soil pollutants, among many other applications. However, even though aTF-based biosensors have been established as successful tools for bioengineering and remediation, rational engineering of aTF small molecule-specificity have so far not been demonstrated, highlighting the need for a deeper understanding of the sequence-function relationships that govern aTF allostery. Here, by combining directed evolution of a naïve library of VanR, a vanillic acid transcriptional regulator from Caulobacter crescentus in yeast, followed by saturation mutagenesis of selected positions we identify residues required for vanillic acid responsiveness, while at the same time maintaining responsiveness to vanillin. Selected single-position VanR mutants show both complete repression of transcription in the absence of any ligand, complete loss of vanillic acid responsiveness, while still maintaining high derepression in the presence of vanillin. By computational ligand docking analyses we also discuss the structure-function relationship single mutations can have on aTF specificity, an attribute potentially accounting for the wide-spread arise of aTF members belonging to the GntR superfamily of transcriptional regulators.</div>","PeriodicalId":100186,"journal":{"name":"Biotechnology Notes","volume":"1 ","pages":"Pages 9-15"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biotno.2020.01.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91684845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Copper-tripeptide complexes for rapid inactivation of Bacillus subtilis endospores 铜-三肽复合物快速灭活枯草芽孢杆菌孢子内

Biotechnology Notes Pub Date : 2020-01-01 DOI: 10.1016/j.biotno.2020.03.002

Yonghao Cui, Kang Zhou, Kun-Lin Yang

引用次数: 1

Improving Spinach2-and Broccoli-based biosensors for single and double analytes 改进基于菠菜2和西兰花的单、双分析生物传感器

Biotechnology Notes Pub Date : 2020-01-01 DOI: 10.1016/j.biotno.2020.01.001

Shuo-Fu Yuan , Hal S. Alper

引用次数: 4

Directed evolution of VanR biosensor specificity in yeast 酵母中VanR生物传感器特异性的定向进化

Biotechnology Notes Pub Date : 2020-01-01 DOI: 10.1016/j.biotno.2020.01.002

Vasil D'ambrosio, S. Pramanik, Kati Goroncy, Tadas Jakočiūnas, David Schönauer, M. Davari, U. Schwaneberg, J. Keasling, M. K. Jensen

引用次数: 15