Ribosome purification from Escherichia coli by ultracentrifugation

Abstract

With more and more researchers conducting extensive research on all aspects of ribosomes, how to extract ribosomes with good effect and high activity has become a fundamental problem. In this article, Escherichia coli A19, MRE600, and JE28 cells often mentioned in the literature and ordinary E. coli BL21(DE3) cells were used to extract ribosomes by ultracentrifugation. The purpose was to study whether the ultracentrifugation method can be applied to extract effective ribosomes, and whether the ribosome extracts from different cells were different. The extracted ribosomes were validated by RNA electrophoresis, SDS-PAGE, PURE system, and mass spectrometry. The validation experiment results showed that ribosomes from these four cells had different effects. The success of the experiment confirmed that effective ribosomes could be extracted from E. coli by ultracentrifugation, which laid a good foundation for researchers to carry out further applications on ribosomes.