Biosensors最新文献_第6页

Quantifying Plant Signaling Pathways by Integrating Luminescence-Based Biosensors and Mathematical Modeling 通过整合发光生物传感器和数学建模量化植物信号通路

Biosensors Pub Date : 2024-08-05 DOI: 10.3390/bios14080378

Shakeel Ahmed, Syed Muhammad Zaigham Abbas Naqvi, Fida Hussain, Muhammad Awais, Yongzhe Ren, Junfeng Wu, Hao Zhang, Yiheng Zang, Jiandong Hu

{"title":"Quantifying Plant Signaling Pathways by Integrating Luminescence-Based Biosensors and Mathematical Modeling","authors":"Shakeel Ahmed, Syed Muhammad Zaigham Abbas Naqvi, Fida Hussain, Muhammad Awais, Yongzhe Ren, Junfeng Wu, Hao Zhang, Yiheng Zang, Jiandong Hu","doi":"10.3390/bios14080378","DOIUrl":"https://doi.org/10.3390/bios14080378","url":null,"abstract":"Plants have evolved intricate signaling pathways, which operate as networks governed by feedback to deal with stressors. Nevertheless, the sophisticated molecular mechanisms underlying these routes still need to be comprehended, and experimental validation poses significant challenges and expenses. Consequently, computational hypothesis evaluation gains prominence in understanding plant signaling dynamics. Biosensors are genetically modified to emit light when exposed to a particular hormone, such as abscisic acid (ABA), enabling quantification. We developed computational models to simulate the relationship between ABA concentrations and bioluminescent sensors utilizing the Hill equation and ordinary differential equations (ODEs), aiding better hypothesis development regarding plant signaling. Based on simulation results, the luminescence intensity was recorded for a concentration of 47.646 RLUs for 1.5 μmol, given the specified parameters and model assumptions. This method enhances our understanding of plant signaling pathways at the cellular level, offering significant benefits to the scientific community in a cost-effective manner. The alignment of these computational predictions with experimental results emphasizes the robustness of our approach, providing a cost-effective means to validate mathematical models empirically. The research intended to correlate the bioluminescence of biosensors with plant signaling and its mathematical models for quantified detection of specific plant hormone ABA.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141939803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sesame Detection in Food Using DNA-Functionalized Gold Nanoparticles: A Sensitive, Rapid, and Cost-Effective Colorimetric Approach 利用 DNA 功能化金纳米粒子检测食品中的芝麻：灵敏、快速、经济的比色法

Biosensors Pub Date : 2024-08-03 DOI: 10.3390/bios14080377

Pablo Llano-Suárez, Adrián Sánchez-Visedo, Inmaculada Ortiz-Gómez, María Teresa Fernández-Argüelles, Marta Prado, José Manuel Costa-Fernández, Ana Soldado

{"title":"Sesame Detection in Food Using DNA-Functionalized Gold Nanoparticles: A Sensitive, Rapid, and Cost-Effective Colorimetric Approach","authors":"Pablo Llano-Suárez, Adrián Sánchez-Visedo, Inmaculada Ortiz-Gómez, María Teresa Fernández-Argüelles, Marta Prado, José Manuel Costa-Fernández, Ana Soldado","doi":"10.3390/bios14080377","DOIUrl":"https://doi.org/10.3390/bios14080377","url":null,"abstract":"Food safety control is a key issue in the food and agriculture industries. For such purposes, developing miniaturized analytical methods is critical for enabling the rapid and sensitive detection of food supplements, allergens, and pollutants. Here, a novel bioanalytical methodology based on DNA-functionalized gold nanoparticles (AuNPs) and colorimetric detection was developed to detect the presence of sesame (a major allergen) through sesame seed DNA as a target, in food samples. The presence of sesame DNA induces controlled nanoparticle aggregation/desegregation, resulting in a color change (from blue to red) proportional to sesame DNA concentration. The incorporation of multicomponent nucleic acid enzymes (MNAzymes) in this strategy has been carried out to perform an isothermal signal amplification strategy to improve the sensitivity of detection. Also, open-source software for color analysis was used to ensure an unbiased visual color-change detection, enhancing detection accuracy and sensitivity and opening the possibility of performing a simple and decentralized analyte detection. The method successfully detected the presence of sesame DNA in sesame seed, sesame oil, olive oil, and sunflower oil. In brief, the developed approach constitutes a simple and affordable alternative to perform a highly sensitive detection of DNA in food without complex methodologies or the requirement of expensive instrumentation.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"133 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141886111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impedance Characteristics of Microfluidic Channels and Integrated Coplanar Parallel Electrodes as Design Parameters for Whole-Channel Analysis in Organ-on-Chip Micro-Systems 微流控通道和集成共面平行电极的阻抗特性是片上器官微系统全通道分析的设计参数

Biosensors Pub Date : 2024-08-01 DOI: 10.3390/bios14080374

Crystal E. Rapier, Srikanth Jagadeesan, Gad D. Vatine, Hadar Ben-Yoav

{"title":"Impedance Characteristics of Microfluidic Channels and Integrated Coplanar Parallel Electrodes as Design Parameters for Whole-Channel Analysis in Organ-on-Chip Micro-Systems","authors":"Crystal E. Rapier, Srikanth Jagadeesan, Gad D. Vatine, Hadar Ben-Yoav","doi":"10.3390/bios14080374","DOIUrl":"https://doi.org/10.3390/bios14080374","url":null,"abstract":"Microfluidics have revolutionized cell culture by allowing for precise physical and chemical environmental control. Coupled with electrodes, microfluidic cell culture can be activated or have its changes sensed in real-time. We used our previously developed reliable and stable microfluidic device for cell growth and monitoring to design, fabricate, and characterize a whole-channel impedance-based sensor and used it to systematically assess the electrical and electrochemical influences of microfluidic channel boundaries coupled with varying electrode sizes, distances, coatings, and cell coverage. Our investigation includes both theoretical and experimental approaches to investigate how design parameters and insulating boundary conditions change impedance characteristics. We examined the system with various solutions using a frequency range of 0.5 Hz to 1 MHz and a modulation voltage of 50 mV. The results show that impedance is directly proportional to electrode distance and inversely proportional to electrode coating, area, and channel size. We also demonstrate that electrode spacing is a dominant factor contributing to impedance. In the end, we summarize all the relationships found and comment on the appropriateness of using this system to investigate barrier cells in blood vessel models and organ-on-a-chip devices. This fundamental study can help in the careful design of microfluidic culture constructs and models that require channel geometries and impedance-based biosensing.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141886113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorogenic RNA-Based Biosensors of Small Molecules: Current Developments, Uses, and Perspectives 基于荧光 RNA 的小分子生物传感器：当前发展、用途和前景

Biosensors Pub Date : 2024-08-01 DOI: 10.3390/bios14080376

Janine Kehrli, Claire Husser, Michael Ryckelynck

引用次数: 0

Recent Advances in Bacterial Detection Using Surface-Enhanced Raman Scattering 利用表面增强拉曼散射检测细菌的最新进展

Biosensors Pub Date : 2024-08-01 DOI: 10.3390/bios14080375

Manal Hassan, Yiping Zhao, Susu M. Zughaier

引用次数: 0

Label-Free Surface-Enhanced Raman Spectroscopy with Machine Learning for the Diagnosis of Thyroid Cancer by Using Fine-Needle Aspiration Liquid Samples 利用细针抽吸液样本进行无标记表面增强拉曼光谱与机器学习以诊断甲状腺癌

Biosensors Pub Date : 2024-07-31 DOI: 10.3390/bios14080372

Lili Gao, Siyi Wu, Puwasit Wongwasuratthakul, Zhou Chen, Wei Cai, Qinyu Li, Linley Li LIN

{"title":"Label-Free Surface-Enhanced Raman Spectroscopy with Machine Learning for the Diagnosis of Thyroid Cancer by Using Fine-Needle Aspiration Liquid Samples","authors":"Lili Gao, Siyi Wu, Puwasit Wongwasuratthakul, Zhou Chen, Wei Cai, Qinyu Li, Linley Li LIN","doi":"10.3390/bios14080372","DOIUrl":"https://doi.org/10.3390/bios14080372","url":null,"abstract":"The incidence of thyroid cancer is increasing worldwide. Fine-needle aspiration (FNA) cytology is widely applied with the use of extracted biological cell samples, but current FNA cytology is labor-intensive, time-consuming, and can lead to the risk of false-negative results. Surface-enhanced Raman spectroscopy (SERS) combined with machine learning algorithms holds promise for cancer diagnosis. In this study, we develop a label-free SERS liquid biopsy method with machine learning for the rapid and accurate diagnosis of thyroid cancer by using thyroid FNA washout fluids. These liquid supernatants are mixed with silver nanoparticle colloids, and dispersed in quartz capillary for SERS measurements to discriminate between healthy and malignant samples. We collect Raman spectra of 36 thyroid FNA samples (18 malignant and 18 benign) and compare four classification models: Principal Component Analysis–Linear Discriminant Analysis (PCA-LDA), Random Forest (RF), Support Vector Machine (SVM), and Convolutional Neural Network (CNN). The results show that the CNN algorithm is the most precise, with a high accuracy of 88.1%, sensitivity of 87.8%, and the area under the receiver operating characteristic curve of 0.953. Our approach is simple, convenient, and cost-effective. This study indicates that label-free SERS liquid biopsy assisted by deep learning models holds great promise for the early detection and screening of thyroid cancer.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141864333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fire up Biosensor Technology to Assess the Vitality of Trees after Wildfires 用生物传感器技术评估野火后树木的生命力

Biosensors Pub Date : 2024-07-31 DOI: 10.3390/bios14080373

Eleftherios Touloupakis, Isabela Calegari Moia, Raffaella Margherita Zampieri, Claudia Cocozza, Niccolò Frassinelli, Enrico Marchi, Cristiano Foderi, Tiziana Di Lorenzo, Negar Rezaie, Valerio Giorgio Muzzini, Maria Laura Traversi, Alessio Giovannelli

{"title":"Fire up Biosensor Technology to Assess the Vitality of Trees after Wildfires","authors":"Eleftherios Touloupakis, Isabela Calegari Moia, Raffaella Margherita Zampieri, Claudia Cocozza, Niccolò Frassinelli, Enrico Marchi, Cristiano Foderi, Tiziana Di Lorenzo, Negar Rezaie, Valerio Giorgio Muzzini, Maria Laura Traversi, Alessio Giovannelli","doi":"10.3390/bios14080373","DOIUrl":"https://doi.org/10.3390/bios14080373","url":null,"abstract":"The development of tools to quickly identify the fate of damaged trees after a stress event such as a wildfire is of great importance. In this context, an innovative approach to assess irreversible physiological damage in trees could help to support the planning of management decisions for disturbed sites to restore biodiversity, protect the environment and understand the adaptations of ecosystem functionality. The vitality of trees can be estimated by several physiological indicators, such as cambium activity and the amount of starch and soluble sugars, while the accumulation of ethanol in the cambial cells and phloem is considered an alarm sign of cell death. However, their determination requires time-consuming laboratory protocols, making the approach impractical in the field. Biosensors hold considerable promise for substantially advancing this field. The general objective of this review is to define a system for quantifying the plant vitality in forest areas exposed to fire. This review describes recent electrochemical biosensors that can detect plant molecules, focusing on biosensors for glucose, fructose, and ethanol as indicators of tree vitality.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141864335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brillouin Biosensing of Viscoelasticity across Phase Transitions in Ovine Cornea 布里渊生物传感技术检测绵羊角膜相变过程中的粘弹性

Biosensors Pub Date : 2024-07-30 DOI: 10.3390/bios14080371

Chingis Kharmyssov, Zhandos Utegulov

{"title":"Brillouin Biosensing of Viscoelasticity across Phase Transitions in Ovine Cornea","authors":"Chingis Kharmyssov, Zhandos Utegulov","doi":"10.3390/bios14080371","DOIUrl":"https://doi.org/10.3390/bios14080371","url":null,"abstract":"Noninvasive in situ monitoring of viscoelastic characteristics of corneal tissue at elevated temperatures is pivotal for mechanical property-informed refractive surgery techniques, including thermokeratoplasty and photorefractive keratectomy, requiring precise thermal modifications of the corneal structure during these surgical procedures. This study harnesses Brillouin light scattering spectroscopy as a biosensing platform to noninvasively probe the viscoelastic properties of ovine corneas across a temperature range of 25–64 °C. By submerging the tissue samples in silicone oil, consistent hydration and immiscibility are maintained, allowing for their accurate sensing of temperature-dependent mechanical behaviors. We identify significant phase transitions in the corneal tissue, particularly beyond 40 °C, likely due to collagen unfolding, marking the beginning of thermal destabilization. A subsequent transition, observed beyond 60 °C, correlates with collagen denaturation. These phase transformations highlight the cornea’s sensitivity to both physiologically reversible and irreversible viscoelastic changes induced by mild to high temperatures. Our findings underscore the potential of the Brillouin biosensing technique for real-time diagnostics of corneal biomechanics during refractive surgeries to attain optimized therapeutic outcomes.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141864341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using AuNPs-DNA Walker with Fluorophores Detects the Hepatitis Virus Rapidly 利用带有荧光团的 AuNPs-DNA Walker 快速检测肝炎病毒

Biosensors Pub Date : 2024-07-29 DOI: 10.3390/bios14080370

Baining Sun, Chenxiang Zheng, Dun Pan, Leer Shen, Wan Zhang, Xiaohua Chen, Yanqin Wen, Yongyong Shi

{"title":"Using AuNPs-DNA Walker with Fluorophores Detects the Hepatitis Virus Rapidly","authors":"Baining Sun, Chenxiang Zheng, Dun Pan, Leer Shen, Wan Zhang, Xiaohua Chen, Yanqin Wen, Yongyong Shi","doi":"10.3390/bios14080370","DOIUrl":"https://doi.org/10.3390/bios14080370","url":null,"abstract":"Viral hepatitis is a systemic infectious diseases caused by various hepatitis viruses, primarily leading to liver damage. It is widely prevalent worldwide, with hepatitis viruses categorized into five types: hepatitis A, B, C, D, and E, based on their etiology. Currently, the detection of hepatitis viruses relies on methods such as enzyme-linked immunosorbent assay (ELISA), immunoelectron microscopy to observe and identify viral particles, and in situ hybridization to detect viral DNA in tissues. However, these methods have limitations, including low sensitivity, high error rates in results, and potential false negative reactions due to occult serum infection conditions. To address these challenges, we have designed an AuNPs-DNA walker method that uses gold nanoparticles (AuNPs) and complementary DNA strands for detecting viral DNA fragments through a colorimetric assay and fluorescence detection. The DNA walker, attached to gold nanoparticles, comprises a long walking strand with a probe sequence bound and stem-loop structural strands featuring a modified fluorescent molecule at the 3′ end, which contains the DNAzyme structural domain. Upon the addition of virus fragments, the target sequence binds to the probe chains. Subsequently, the long walking strand is released and continuously hybridizes with the stem-loop structural strand. The DNAzyme undergoes hydrolytical cleavage by Mg2+, breaking the stem-loop structural strand into linear single strands. As a result of these structural changes, the negative charge density in the solution decreases, weakening spatial repulsion and rapidly reducing the stability of the DNA walker. This leads to aggregation upon the addition of a high-salt solution, accompanied by a color change. Virus typing can be performed through fluorescence detection. The innovative method can detect DNA/RNA fragments with high specificity for the target sequence, reaching concentrations as low as 1 nM. Overall, our approach offers a more convenient and reliable method for the detection of hepatitis viruses.","PeriodicalId":100185,"journal":{"name":"Biosensors","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141864337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a NIR Iridium(III) Complex-Based Probe for the Selective Detection of Iron(II) Ions 开发用于选择性检测铁(II)离子的近红外铱(III)络合物探针

Biosensors Pub Date : 2024-07-29 DOI: 10.3390/bios14080369

Wanyi Wang, Zixi Zhang, Jingqi Liu, Lingtan Kong, Wanhe Wang, Chung-Hang Leung, Jing Wang

引用次数: 0