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Electric birefringence of actin 肌动蛋白的电双折射
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90096-8
Syoyu Kobayashi, Hiroshi Asai, Fumio Oosawa
{"title":"Electric birefringence of actin","authors":"Syoyu Kobayashi,&nbsp;Hiroshi Asai,&nbsp;Fumio Oosawa","doi":"10.1016/0926-6577(64)90096-8","DOIUrl":"10.1016/0926-6577(64)90096-8","url":null,"abstract":"<div><p>The polymerization of actin was followed by the measurement of electric birefringence. Small elementary polymers of actin which appeared at very low salt concentrations or at the initial stage of polymerization showed a positive electric birefringence. They were oriented with the long axis parallel to the electric field. This was mainly due to a permanent dipole moment in the direction of the long axis, the magnitude of which was estimated from the Kerr constant to be about 75 Debye units per actin monomer. The elementary polymers grew into normal F-actin which showed a large negative electric birefringence. That is, F-actin filaments were oriented in the direction perpendicular to the field. Therefore, the electric structure of F-actin filaments was completely different from that of elementary small polymers. The negative electric birefringence of F-actin was cancelled by the addition of H-meromyosin.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90096-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23802383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 61
Observations on the fluorescence emitted by slices of rat liver and avian salt gland 大鼠肝脏和鸟盐腺切片荧光观察
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90094-4
G.D.V. Van Rossum
{"title":"Observations on the fluorescence emitted by slices of rat liver and avian salt gland","authors":"G.D.V. Van Rossum","doi":"10.1016/0926-6577(64)90094-4","DOIUrl":"10.1016/0926-6577(64)90094-4","url":null,"abstract":"<div><p>The fluorescence emitted by slices of rat liver and sea-gull salt gland which were irradiated with light at 366 mμ, showed a peak at 468 mμ under aerobic conditions. The intensity of the fluorescence decreased during the early stages of incubation of 38°, due largely to the effects of the ultraviolet exciting radiation. Under aeobic conditions of incubation the fluorescence intensity fell by 40–60, and the remained relatively constant for a period of several hours; in anaerobic conditions the fall in intensity continued to lower levels. During the period in which the fluorescence of aerobic slices constant, quantitatively reproducible increases in tissue fluorescence were caused by the onset of tempory anoxia, by the addition of succinate to the medium and by the treatments which stimulated ion transport in the slices.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90094-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72908467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Studies on ion transport 离子输运研究
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90107-X
G.P. Brierley, E. Murer, R.L. O'Brien
{"title":"Studies on ion transport","authors":"G.P. Brierley,&nbsp;E. Murer,&nbsp;R.L. O'Brien","doi":"10.1016/0926-6577(64)90107-X","DOIUrl":"10.1016/0926-6577(64)90107-X","url":null,"abstract":"","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90107-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87197672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Potassium transport in Neurospora 钾在神经孢子虫中的转运
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90101-9
Carolyn W. Slayman , E.L. Tatum
{"title":"Potassium transport in Neurospora","authors":"Carolyn W. Slayman ,&nbsp;E.L. Tatum","doi":"10.1016/0926-6577(64)90101-9","DOIUrl":"10.1016/0926-6577(64)90101-9","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Wild-type <em>Neurospora crassa</em>, growing logarithmically in minimal medium, contains 180 ± 16 mM intracellular K<sup>+</sup> and 14 ± 2 mM <em>Na</em><sup>+</sup>. It requires K<sup>+</sup> but not Na<sup>+</sup> for growth. Over a wide range of extracellular K<sup>+</sup> concentrations (0.3 to 100 mM), the cells maintain a constant, high level of intracellular K<sup>+</sup> (180 mM) and rapid growth occurs. Below 0.3 mM, the internal K<sup>+</sup> concentration falls and growth is inhibited.</p></span></li><li><span>2.</span><span><p>2. When cells are harvested from logarithmic growth and resuspended in distilled water or in 1 mM sodium azide, they show no significant loss of internal K<sup>+</sup> for at least 1 h. Exposure to the polyene antibiotic nystatin (30 μg/ml) or the decapeptide antibiotic tyrocidine (20 μg/ml) causes most of the internal K<sup>+</sup> to be lost within 2 min. Both antibiotics are thought to attack the plasma membrane of sensitive organisms.</p></span></li><li><span>3.</span><span><p>3. Neurospora exchanges its intracellular K<sup>+</sup> quite rapidly for extracellular K<sup>+</sup> or Rb<sup>+</sup>, but only slowly for Na<sup>+</sup>.</p></span></li><li><span>4.</span><span><p>4. These experiments were undertaken to provide a backgroun for microelectrode studies with Neurospora and for the isolation of a K<sup>+</sup>-transport mutant.</p></span></li></ul></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90101-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81540387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 135
The inhibition of transport of glucose into human erythrocytes by 2,3,4,6-tetra[O-acethyl]-β-d-glucopyranose 2,3,4,6-四[o -乙基]-β-d-葡萄糖吡喃糖对葡萄糖转运到人红细胞的抑制作用
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90099-3
L. Lacko, M. Burger
{"title":"The inhibition of transport of glucose into human erythrocytes by 2,3,4,6-tetra[O-acethyl]-β-d-glucopyranose","authors":"L. Lacko,&nbsp;M. Burger","doi":"10.1016/0926-6577(64)90099-3","DOIUrl":"10.1016/0926-6577(64)90099-3","url":null,"abstract":"<div><p><span><math><mtext>2,3,4,6-</mtext><mtext>tetra</mtext><mtext>[O-</mtext><mtext>acetyl</mtext><mtext>]-β-</mtext><mtext>d</mtext><mtext>-</mtext><mtext>glucopyranose</mtext></math></span> penetrated into human erythrocytes following a transport mechanism different from that of glucose. However, TAG interacted with the glucose-carrier ssytem, inhibiting the transport of glucose to a variable extent, depending on experimental conditions.</p><p>Comparison of the inhibitory effect of TAG on exchange and non-exchange transport showed that it affected a suppression of glucose transport similar to that obtained by lowering the temperature.</p><p>The presence of subtances in the matrix of the membrane which regulate the movement of carriers, interacting similarly to TAG, is tentatively suggested.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90099-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23802386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optical absorption of the porphyrin free radical formed in a reversible photochemical reaction 光吸收卟啉自由基形成的可逆光化学反应
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90112-3
D. Mauzerall , G. Feher
{"title":"Optical absorption of the porphyrin free radical formed in a reversible photochemical reaction","authors":"D. Mauzerall ,&nbsp;G. Feher","doi":"10.1016/0926-6577(64)90112-3","DOIUrl":"10.1016/0926-6577(64)90112-3","url":null,"abstract":"","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90112-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23802397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 20
Transport of neutral, dibasic and N-methyl-substituted amino acids by rat intestine 中性、二碱性和n -甲基取代氨基酸在大鼠肠内的转运
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90100-7
P. Reed Larsen, Joan E. Ross, Donald F. Tapley
{"title":"Transport of neutral, dibasic and N-methyl-substituted amino acids by rat intestine","authors":"P. Reed Larsen,&nbsp;Joan E. Ross,&nbsp;Donald F. Tapley","doi":"10.1016/0926-6577(64)90100-7","DOIUrl":"10.1016/0926-6577(64)90100-7","url":null,"abstract":"<div><p>The ability of everted sacs of rat small intestine to transport neutral, dibasic and <em>N</em>-methyl-substituted amino acids has been determined. The data obtained support the suggestion that three separate amino acid-transport systems are present in rat intestine.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90100-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23802387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 84
The macromolecular parameters of ascites tumor cell H-RNA 腹水肿瘤细胞H-RNA的大分子参数
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90105-6
S.N. Timasheff
{"title":"The macromolecular parameters of ascites tumor cell H-RNA","authors":"S.N. Timasheff","doi":"10.1016/0926-6577(64)90105-6","DOIUrl":"10.1016/0926-6577(64)90105-6","url":null,"abstract":"<div><p>A concerted analysis of sedimentation, light scattering and small-angle X-ray-scattering data on high-molecular-weight Ehrlich ascites tumor cell RNA has been carried out. The three sets of data have been found to be in quantitative agreement with a compact structure consisting of short double-helical roldlike subunits, which account for 90% of the RNA mass.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90105-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23803754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Biochimica et biophysica acta, vol. 88 (1964) 生物化学与生物物理学报》，第 88 卷（1964 年）
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90117-2
{"title":"Biochimica et biophysica acta, vol. 88 (1964)","authors":"","doi":"10.1016/0926-6577(64)90117-2","DOIUrl":"https://doi.org/10.1016/0926-6577(64)90117-2","url":null,"abstract":"","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90117-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136455413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Photodimerization of urocanic acid in vitro and in vivo 尿尿酸在体外和体内的光二聚化
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90092-0
J.H. Anglin Jr., Mark Allen Everett
{"title":"Photodimerization of urocanic acid in vitro and in vivo","authors":"J.H. Anglin Jr.,&nbsp;Mark Allen Everett","doi":"10.1016/0926-6577(64)90092-0","DOIUrl":"10.1016/0926-6577(64)90092-0","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Irradition of urocanic acid or urocanate frozen in aqueous solution with 280–320-mμ light produced a photodimer which was isolated by gradient elution from an anion-exchange resin.</p></span></li><li><span>2.</span><span><p>2. A similar substance was shown to be present in dilute acid extracts of guinea-pig epidermis after irradiation with 280–320-mμ light.</p></span></li><li><span>3.</span><span><p>3. The photodimer formed with 280–320-mμ light when irradiated in solution with 254-mμ light was converted to urocanic acid and smaller amounts of several other products.</p></span></li><li><span>4.</span><span><p>4. Irradiation of crystalline urocanic acid or its solutions does not produce an accumulation of the photodimer.</p></span></li></ul></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90092-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23802379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
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