Electric birefringence of actin

Syoyu Kobayashi, Hiroshi Asai, Fumio Oosawa
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引用次数: 61

Abstract

The polymerization of actin was followed by the measurement of electric birefringence. Small elementary polymers of actin which appeared at very low salt concentrations or at the initial stage of polymerization showed a positive electric birefringence. They were oriented with the long axis parallel to the electric field. This was mainly due to a permanent dipole moment in the direction of the long axis, the magnitude of which was estimated from the Kerr constant to be about 75 Debye units per actin monomer. The elementary polymers grew into normal F-actin which showed a large negative electric birefringence. That is, F-actin filaments were oriented in the direction perpendicular to the field. Therefore, the electric structure of F-actin filaments was completely different from that of elementary small polymers. The negative electric birefringence of F-actin was cancelled by the addition of H-meromyosin.

肌动蛋白的电双折射
在肌动蛋白聚合后进行电双折射测量。在极低盐浓度或聚合初始阶段出现的肌动蛋白小的基本聚合物表现出正的电双折射。它们以平行于电场的长轴定向。这主要是由于长轴方向上的永久偶极矩,其大小由克尔常数估计为每个肌动蛋白单体约75德拜单位。基本聚合物生长成正常的f -肌动蛋白,表现出较大的负电双折射。也就是说,f -肌动蛋白丝在垂直于场的方向上定向。因此,f -肌动蛋白丝的电结构与基本小聚合物完全不同。F-actin的负电双折射被H-meromyosin的加入所抵消。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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