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Chlorophylls of photosynthetic bacteria 光合细菌的叶绿素
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90089-0
Arne Jensen, Olav Aasmundrud , Kjell E. Eimhjellen
{"title":"Chlorophylls of photosynthetic bacteria","authors":"Arne Jensen,&nbsp;Olav Aasmundrud ,&nbsp;Kjell E. Eimhjellen","doi":"10.1016/0926-6577(64)90089-0","DOIUrl":"10.1016/0926-6577(64)90089-0","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The chlorophylls and the corresponding phaeophytins of 18 species of photosynthetic bacteria have been characterized by paper chromatography and by absorption spectra in visible light.</p></span></li><li><span>2.</span><span><p>2. Except for one species of Rhodopseudomonas which contained bacteriochlorophyll <em>b</em>, all the Thiorhodaceae (four) and the Athiorhodaceae (nine) species investigated as well as <em>Rhodomicrobium vannielii</em> had bacteriochlorophyll <em>a</em> as the only detectable chlorophyll.</p></span></li><li><span>3.</span><span><p>3. The chlorophyll-770 of Chlorobacteriaceae (four strains or species) was found to be spectroscopically and chromatographically identical with bacteriochlorophyll <em>a</em>.</p></span></li><li><span>4.</span><span><p>4. The chlorobium chlorophylls-650 and -660 were each separated by paper chromatography into three fractions. In the case of chlorobium chlorophyll-650 two of the fractions each gave rise to one phaeophytin, whereas the last fraction gave three phaeophytins. All phaeophytins had identical absorption spectra, but exhibited different <em>R</em><sub><em>F</em></sub>-values on paper chromatograms.</p></span></li><li><span>5.</span><span><p>5. In addition to the chlorophylls mentioned above, all the species of the Chlorobacteriaceae contained trace amounts of a hitherto undescribed chlorophyllous pigment.</p></span></li><li><span>6.</span><span><p>6. A revision of the current nomenclature for bacterial chlorophyll is proposed.</p></span></li></ul></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 3","pages":"Pages 466-479"},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90089-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23800716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 84
Studies on the surface and cytoplasmic membranes of Ehrlich ascites carcinoma cells 埃利希腹水癌细胞表面及细胞质膜的研究
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90104-4
Donald F. Hoelzl Wallach, Donna Ullrey
{"title":"Studies on the surface and cytoplasmic membranes of Ehrlich ascites carcinoma cells","authors":"Donald F. Hoelzl Wallach,&nbsp;Donna Ullrey","doi":"10.1016/0926-6577(64)90104-4","DOIUrl":"10.1016/0926-6577(64)90104-4","url":null,"abstract":"<div><p>A membrane fraction isolated from Ehrlich ascites carcinoma microsomes contians an ATP phosphohydrolas which is strongly stimulated by K<sup>+</sup> in the presence of Na<sup>+</sup>. The enzyme system has an absolute requirement for Na<sup>+</sup> and Mg<sup>2+</sup>, but a number of monovalent cations can simulate K<sup>+</sup>. Ca<sup>2+</sup>, ADP and ouabain are inhibitory. The enzyme is believed to be associated with plasma membrane fragments.</p><p>The quantitative relationships between enzyme activity and reactant concentration is presented and the probable relationship between ion transport in the whole cell and the alkali-cation-sensitive ATP phosphohydrolase is discussed.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 3","pages":"Pages 620-629"},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90104-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23802391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 35
Photoreduction of nicotinamide-adenine dinucleotide by a cell-free system from Chromatium 用无细胞系统从Chromatium中光还原烟酰胺-腺嘌呤二核苷酸
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90088-9
S.L. Hood
{"title":"Photoreduction of nicotinamide-adenine dinucleotide by a cell-free system from Chromatium","authors":"S.L. Hood","doi":"10.1016/0926-6577(64)90088-9","DOIUrl":"10.1016/0926-6577(64)90088-9","url":null,"abstract":"<div><p>A small particle fraction has been prepared from <em>Chromatium</em> which reduces NAD by both photo and dark reactions. The reductions are stimulated by the addition of two <em>Chromatium</em> enzymes, a flavoprotein and cyrstalline ferredoxin.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 3","pages":"Pages 461-465"},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90088-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23800715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Evidence of bacteriochlorophyll dimerization in the chlorophyll-protein complex from green photosynthetic bacteria 绿色光合细菌叶绿素-蛋白质复合体中细菌叶绿素二聚化的证据
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90113-5
John M. Olson
{"title":"Evidence of bacteriochlorophyll dimerization in the chlorophyll-protein complex from green photosynthetic bacteria","authors":"John M. Olson","doi":"10.1016/0926-6577(64)90113-5","DOIUrl":"10.1016/0926-6577(64)90113-5","url":null,"abstract":"","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 3","pages":"Pages 660-662"},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90113-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23802398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Inhibition of greening of heat-bleached Euglena by streptomycin 链霉素对热漂绿藻的抑制作用
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90114-7
John L. Mego
{"title":"Inhibition of greening of heat-bleached Euglena by streptomycin","authors":"John L. Mego","doi":"10.1016/0926-6577(64)90114-7","DOIUrl":"10.1016/0926-6577(64)90114-7","url":null,"abstract":"","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 3","pages":"Pages 663-665"},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90114-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23802399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Influence of cysteine on the photochemical decomposition of thymidyl-(5′-3′)-bromodeoxyuridine 半胱氨酸对胸苷基-(5 ' -3 ')-溴脱氧尿苷光化学分解的影响
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90090-7
A. Haug
{"title":"Influence of cysteine on the photochemical decomposition of thymidyl-(5′-3′)-bromodeoxyuridine","authors":"A. Haug","doi":"10.1016/0926-6577(64)90090-7","DOIUrl":"10.1016/0926-6577(64)90090-7","url":null,"abstract":"<div><p>Irradiating a solution of thymidyl-(5′-3′)-bromodeoxyuridine with monochromatic, ultraviolet light yields one main photo-product, an intramolecular dimer, containing no Br and a cyclobutene ring structure. In the presence of cysteine and ultraviolet radiation the dimer is further decomposed to a photo-product characterized by an end-absorption spectrum. This compound contains one S atom per dinucleotide and cannot be reverted by irradiation at 2400 Å. The quantum yields of this sulfhydryl addition are measured as a function of wavelength. The reaction kinetics and the biological implication of this process, which is possibly radio-protective, are discussed.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 3","pages":"Pages 480-486"},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90090-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87755988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Volume changes in isolated myofibrils 离体肌原纤维的体积变化
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90095-6
R.J. Baskin
{"title":"Volume changes in isolated myofibrils","authors":"R.J. Baskin","doi":"10.1016/0926-6577(64)90095-6","DOIUrl":"10.1016/0926-6577(64)90095-6","url":null,"abstract":"<div><p>Volume changes in glycerol-extracted myofibrils were studied. The effect of pH, ion content and ATP concentration on the change in volume was determined. A volume change greater than that attributed to ATP dephosphorylation was observed. The magnitude of the volume change of the entire system was 10<sup>−3</sup> cm<sup>3</sup> whereas that due to dephosphorylation was 10<sup>−6</sup> cm<sup>3</sup>. The ratio of ATPase activity in a volume decrease to that in a volume increase was found to be approx. 10:1. The total amount of volume decrease measured approx. 0.028 cm<sup>3</sup>/g of muscle with a fresh myo fibril preparation. The volume increase which was observed upon the addition of large amounts of ATP amounted to approx. 0.036 cm<sup>3</sup>/g of muscle. The relationship between the volume changes and molecular reorganization of the myofibril proteins was discussed. It was concluded that either a large configurational change occurs in the myofibril proteins during an ATP-induced contraction or relaxation, or that the volume changes are related to the dissociation and association of actin and myosin filaments</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 3","pages":"Pages 517-527"},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90095-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23802382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Electric birefringence of actin 肌动蛋白的电双折射
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90096-8
Syoyu Kobayashi, Hiroshi Asai, Fumio Oosawa
{"title":"Electric birefringence of actin","authors":"Syoyu Kobayashi,&nbsp;Hiroshi Asai,&nbsp;Fumio Oosawa","doi":"10.1016/0926-6577(64)90096-8","DOIUrl":"10.1016/0926-6577(64)90096-8","url":null,"abstract":"<div><p>The polymerization of actin was followed by the measurement of electric birefringence. Small elementary polymers of actin which appeared at very low salt concentrations or at the initial stage of polymerization showed a positive electric birefringence. They were oriented with the long axis parallel to the electric field. This was mainly due to a permanent dipole moment in the direction of the long axis, the magnitude of which was estimated from the Kerr constant to be about 75 Debye units per actin monomer. The elementary polymers grew into normal F-actin which showed a large negative electric birefringence. That is, F-actin filaments were oriented in the direction perpendicular to the field. Therefore, the electric structure of F-actin filaments was completely different from that of elementary small polymers. The negative electric birefringence of F-actin was cancelled by the addition of H-meromyosin.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 3","pages":"Pages 528-540"},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90096-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23802383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 61
Observations on the fluorescence emitted by slices of rat liver and avian salt gland 大鼠肝脏和鸟盐腺切片荧光观察
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90094-4
G.D.V. Van Rossum
{"title":"Observations on the fluorescence emitted by slices of rat liver and avian salt gland","authors":"G.D.V. Van Rossum","doi":"10.1016/0926-6577(64)90094-4","DOIUrl":"10.1016/0926-6577(64)90094-4","url":null,"abstract":"<div><p>The fluorescence emitted by slices of rat liver and sea-gull salt gland which were irradiated with light at 366 mμ, showed a peak at 468 mμ under aerobic conditions. The intensity of the fluorescence decreased during the early stages of incubation of 38°, due largely to the effects of the ultraviolet exciting radiation. Under aeobic conditions of incubation the fluorescence intensity fell by 40–60, and the remained relatively constant for a period of several hours; in anaerobic conditions the fall in intensity continued to lower levels. During the period in which the fluorescence of aerobic slices constant, quantitatively reproducible increases in tissue fluorescence were caused by the onset of tempory anoxia, by the addition of succinate to the medium and by the treatments which stimulated ion transport in the slices.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 3","pages":"Pages 507-516"},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90094-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72908467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
The inhibition of transport of glucose into human erythrocytes by 2,3,4,6-tetra[O-acethyl]-β-d-glucopyranose 2,3,4,6-四[o -乙基]-β-d-葡萄糖吡喃糖对葡萄糖转运到人红细胞的抑制作用
Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-11-29 DOI: 10.1016/0926-6577(64)90099-3
L. Lacko, M. Burger
{"title":"The inhibition of transport of glucose into human erythrocytes by 2,3,4,6-tetra[O-acethyl]-β-d-glucopyranose","authors":"L. Lacko,&nbsp;M. Burger","doi":"10.1016/0926-6577(64)90099-3","DOIUrl":"10.1016/0926-6577(64)90099-3","url":null,"abstract":"<div><p><span><math><mtext>2,3,4,6-</mtext><mtext>tetra</mtext><mtext>[O-</mtext><mtext>acetyl</mtext><mtext>]-β-</mtext><mtext>d</mtext><mtext>-</mtext><mtext>glucopyranose</mtext></math></span> penetrated into human erythrocytes following a transport mechanism different from that of glucose. However, TAG interacted with the glucose-carrier ssytem, inhibiting the transport of glucose to a variable extent, depending on experimental conditions.</p><p>Comparison of the inhibitory effect of TAG on exchange and non-exchange transport showed that it affected a suppression of glucose transport similar to that obtained by lowering the temperature.</p><p>The presence of subtances in the matrix of the membrane which regulate the movement of carriers, interacting similarly to TAG, is tentatively suggested.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 3","pages":"Pages 564-569"},"PeriodicalIF":0.0,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90099-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23802386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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